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P02563

- MYH6_RAT

UniProt

P02563 - MYH6_RAT

Protein

Myosin-6

Gene

Myh6

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 2 (01 Oct 1989)
      Previous versions | rss
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    Functioni

    Muscle contraction.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi177 – 1848ATP

    GO - Molecular functioni

    1. ATP binding Source: RGD
    2. calcium-dependent ATPase activity Source: RGD
    3. identical protein binding Source: IntAct
    4. microfilament motor activity Source: RGD
    5. protein heterodimerization activity Source: RGD
    6. protein homodimerization activity Source: RGD

    GO - Biological processi

    1. ATP catabolic process Source: RGD
    2. muscle contraction Source: RGD
    3. response to heat Source: RGD

    Keywords - Molecular functioni

    Motor protein, Muscle protein, Myosin

    Keywords - Ligandi

    Actin-binding, ATP-binding, Calmodulin-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Myosin-6
    Alternative name(s):
    Myosin heavy chain 6
    Myosin heavy chain, cardiac muscle alpha isoform
    Short name:
    MyHC-alpha
    Gene namesi
    Name:Myh6
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi62029. Myh6.

    Subcellular locationi

    Cytoplasmmyofibril
    Note: Thick filaments of the myofibrils.

    GO - Cellular componenti

    1. cytoplasm Source: RGD
    2. mitochondrion Source: RGD
    3. muscle myosin complex Source: RGD
    4. myofibril Source: RGD
    5. myosin filament Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cytoplasm, Thick filament

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 19381938Myosin-6PRO_0000123404Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei128 – 1281N6,N6,N6-trimethyllysineSequence Analysis
    Cross-linki205 – 205Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
    Cross-linki206 – 206Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
    Cross-linki213 – 213Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
    Cross-linki1280 – 1280Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
    Cross-linki1538 – 1538Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
    Cross-linki1570 – 1570Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

    Keywords - PTMi

    Isopeptide bond, Methylation, Ubl conjugation

    Proteomic databases

    PaxDbiP02563.
    PRIDEiP02563.

    PTM databases

    PhosphoSiteiP02563.

    Expressioni

    Gene expression databases

    GenevestigatoriP02563.

    Interactioni

    Subunit structurei

    Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself6EBI-6122328,EBI-6122328

    Protein-protein interaction databases

    DIPiDIP-41048N.
    IntActiP02563. 1 interaction.
    MINTiMINT-348910.
    STRINGi10116.ENSRNOP00000023301.

    Structurei

    3D structure databases

    ProteinModelPortaliP02563.
    SMRiP02563. Positions 839-964.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini84 – 779696Myosin motorAdd
    BLAST
    Domaini782 – 81130IQPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni656 – 67823Actin-bindingAdd
    BLAST
    Regioni758 – 77215Actin-bindingAdd
    BLAST
    Regioni789 – 80618Calmodulin-bindingBy similarityAdd
    BLAST
    Regioni815 – 83218Calmodulin-bindingBy similarityAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili842 – 19381097Sequence AnalysisAdd
    BLAST

    Domaini

    The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).

    Sequence similaritiesi

    Contains 1 IQ domain.PROSITE-ProRule annotation
    Contains 1 myosin motor domain.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG5022.
    HOGENOMiHOG000173959.
    HOVERGENiHBG004704.
    InParanoidiP02563.
    PhylomeDBiP02563.

    Family and domain databases

    Gene3Di4.10.270.10. 1 hit.
    InterProiIPR000048. IQ_motif_EF-hand-BS.
    IPR027401. Myosin-like_IQ_dom.
    IPR001609. Myosin_head_motor_dom.
    IPR004009. Myosin_N.
    IPR002928. Myosin_tail.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00063. Myosin_head. 1 hit.
    PF02736. Myosin_N. 1 hit.
    PF01576. Myosin_tail_1. 1 hit.
    [Graphical view]
    PRINTSiPR00193. MYOSINHEAVY.
    SMARTiSM00015. IQ. 2 hits.
    SM00242. MYSc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS50096. IQ. 1 hit.
    PS51456. MYOSIN_MOTOR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P02563-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTDAQMADFG AARYLRKSEK ERLEAQTRPF DIRTECFVPD DKEEYVKAKI     50
    VSREGGKVTA ETENGKTVTV KEDQVMQQNP PKFDKIEDMA MLTFLHEPAV 100
    LYNLKERYAA WMIYTYSGLF CVTVNPYKWL PVYNAEVVAA YRGKKRSEAP 150
    PHIFSISDNA YQYMLTDREN QSILITGESG AGKTVNTKRV IQYFASIAAI 200
    GDRSKKDNPN ANKGTLEDQI IQANPALEAF GNAKTVRNDN SSRFGKFIRI 250
    HFGATGKLAS ADIETYLLEK SRVIFQLKAE RNYHIFYQIL SNKKPELLDM 300
    LLVTNNPYDY AFVSQGEVSV ASIDDSEELL ATDSAFDVLG FTAEEKAGVY 350
    KLTGAIMHYG NMKFKQKQRE EQAEPDGTED ADKSAYLMGL NSADLLKGLC 400
    HPRVKVGNEY VTKGQSVQQV YYSIGALAKS VYEKMFNWMV TRINATLETK 450
    QPRQYFIGVL DIAGFEIFDF NSFEQLCINF TNEKLQQFFN HHMFVLEQEE 500
    YKKEGIEWEF IDFGMDLQAC IDLIEKPMGI MSILEEECMF PKATDMTFKA 550
    KLYDNHLGKS NNFQKPRNVK GKQEAHFSLV HYAGTVDYNI LGWLEKNKDP 600
    LNETVVGLYQ KSSLKLMATL FSTYASADTG DSGKGKGGKK KGSSFQTVSA 650
    LHRENLNKLM TNLRTTHPHF VRCIIPNERK APGVMDNPLV MHQLRCNGVL 700
    EGIRICRKGF PNRILYGDFR QRYRILNPAA IPEGQFIDSG KGAEKLLGSL 750
    DIDHNQYKFG HTKVFFKAGL LGLLEEMRDE RLSRIITRIQ AQARGQLMRI 800
    EFKKMVERRD ALLVIQWNIR AFMGVKNWPW MKLYFKIKPL LKSAETEKEM 850
    ANMKEEFGRV KDALEKSEAR RKELEEKMVS LLQEKNDLQL QVQAEQDNLA 900
    DAEERCDQLI KNKIQLEAKV KEMTERLEDE EEMNAELTAK KRKLEDECSE 950
    LKKDIDDLEL TLAKVEKEKH ATENKVKNLT EEMAGLDEII AKLTKEKKAL 1000
    QEAHQQALDD LQAEEDKVNT LTKSKVKLEQ QVDDLEGSLE QEKKVRMDLE 1050
    RAKRKLEGDL KLTQESIMDL ENDKLQLEEK LKKKEFDISQ QNSKIEDEQA 1100
    LALQLQKKLK ENQARIEELE EELEAERTAR AKVEKLRSDL TRELEEISER 1150
    LEEAGGATSV QIEMNKKREA EFQKMRRDLE EATLQHEATA AALRKKHADS 1200
    VAELGEQIDN LQRVKQKLEK EKSEFKLELD DVTSHMEQII KAKANLEKVS 1250
    RTLEDQANEY RVKLEEAQRS LNDFTTQRAK LQTENGELAR QLEEKEALIW 1300
    QLTRGKLSYT QQMEDLKRQL EEEGKAKNAL AHALQSARHD CDLLREQYEE 1350
    EMEAKAELQR VLSKANSEVA QWRTKYETDA IQRTEELEEA KKKLAQRLQD 1400
    AEEAVEAVNA KCSSLEKTKH RLQNEIEDLM VDVERSNAAA AALDKKQRNF 1450
    DKILAEWKQK YEESQSELES SQKEARSLST ELFKLKNAYE ESLEHLETFK 1500
    RENKNLQEEI SDLTEQLGEG GKNVHELEKI RKQLEVEKLE LQSALEEAEA 1550
    SLEHEEGKIL RAQLEFNQIK AEIERKLAEK DEEMEQAKRN HLRVVDSLQT 1600
    SLDAETRSRN EALRVKKKME GDLNEMEIQL SQANRIASEA QKHLKNAQAH 1650
    LKDTQLQLDD AVRANDDLKE NIAIVERRNT LLQAELEELR AVVEQTERSR 1700
    KLAEQELIET SERVQLLHSQ NTSLINQKKK MDADLSQLQT EVEEAVQECR 1750
    NAEEKAKKAI TDAAMMAEEL KKEQDTSAHL ERMKKNMEQT IKDLQHRLDE 1800
    AEQIALKGGK KQLQKLEARV RELENELEAE QKRNAESVKG MRKSERRIKE 1850
    LTYQTEEDKK NLVRLQDLVD KLQLKVKAYK RQAEEAEEQA NTNLSKFRKV 1900
    QHELDEAEER ADIAESQVNK LRAKSRDIGA KQKMHDEE 1938
    Length:1,938
    Mass (Da):223,508
    Last modified:October 1, 1989 - v2
    Checksum:iD7BD33FC2B19E3C2
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti13 – 131R → AP(PubMed:6585819)Curated
    Sequence conflicti46 – 461V → A in AAA41648. (PubMed:6585819)Curated
    Sequence conflicti51 – 522VS → AP in AAA41648. (PubMed:6585819)Curated
    Sequence conflicti87 – 871E → Q in AAA41648. (PubMed:6585819)Curated
    Sequence conflicti109 – 1091Missing in AAA41648. (PubMed:6585819)Curated
    Sequence conflicti1566 – 15661F → FF(PubMed:7045682)Curated
    Sequence conflicti1575 – 15751R → S in AAA41653. (PubMed:7045682)Curated
    Sequence conflicti1721 – 17211N → T(PubMed:7045682)Curated
    Sequence conflicti1852 – 18521T → N in AAA41653. (PubMed:7045682)Curated
    Sequence conflicti1870 – 18701D → N in AAA41653. (PubMed:7045682)Curated
    Sequence conflicti1934 – 19341M → I(PubMed:7045682)Curated
    Sequence conflicti1934 – 19341M → I(PubMed:6241892)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X15938 mRNA. Translation: CAA34064.1.
    K01464 Genomic DNA. Translation: AAA41648.1.
    J00751 mRNA. Translation: AAA41653.1.
    M32697 mRNA. Translation: AAA41658.1.
    PIRiS06005.
    UniGeneiRn.54399.

    Genome annotation databases

    UCSCiRGD:62029. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X15938 mRNA. Translation: CAA34064.1 .
    K01464 Genomic DNA. Translation: AAA41648.1 .
    J00751 mRNA. Translation: AAA41653.1 .
    M32697 mRNA. Translation: AAA41658.1 .
    PIRi S06005.
    UniGenei Rn.54399.

    3D structure databases

    ProteinModelPortali P02563.
    SMRi P02563. Positions 839-964.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-41048N.
    IntActi P02563. 1 interaction.
    MINTi MINT-348910.
    STRINGi 10116.ENSRNOP00000023301.

    PTM databases

    PhosphoSitei P02563.

    Proteomic databases

    PaxDbi P02563.
    PRIDEi P02563.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:62029. rat.

    Organism-specific databases

    RGDi 62029. Myh6.

    Phylogenomic databases

    eggNOGi COG5022.
    HOGENOMi HOG000173959.
    HOVERGENi HBG004704.
    InParanoidi P02563.
    PhylomeDBi P02563.

    Gene expression databases

    Genevestigatori P02563.

    Family and domain databases

    Gene3Di 4.10.270.10. 1 hit.
    InterProi IPR000048. IQ_motif_EF-hand-BS.
    IPR027401. Myosin-like_IQ_dom.
    IPR001609. Myosin_head_motor_dom.
    IPR004009. Myosin_N.
    IPR002928. Myosin_tail.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00063. Myosin_head. 1 hit.
    PF02736. Myosin_N. 1 hit.
    PF01576. Myosin_tail_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00193. MYOSINHEAVY.
    SMARTi SM00015. IQ. 2 hits.
    SM00242. MYSc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS50096. IQ. 1 hit.
    PS51456. MYOSIN_MOTOR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete nucleotide sequence of full length cDNA for rat alpha cardiac myosin heavy chain."
      Kraft R., Bravo-Zehnder M., Taylor D., Leinwand L.A.
      Nucleic Acids Res. 17:7527-7528(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Heart.
    2. "Full-length rat alpha and beta cardiac myosin heavy chain sequences. Comparisons suggest a molecular basis for functional differences."
      McNally E.M., Kraft R., Bravo-Zehnder M., Taylor D., Leinwand L.A.
      J. Mol. Biol. 210:665-671(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISCUSSION OF SEQUENCE.
    3. "Cardiac alpha- and beta-myosin heavy chain genes are organized in tandem."
      Mahdavi V., Chambers A.P., Nadal-Ginard B.
      Proc. Natl. Acad. Sci. U.S.A. 81:2626-2630(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-167.
    4. "Molecular characterization of two myosin heavy chain genes expressed in the adult heart."
      Mahdavi V., Periasamy M., Nadal-Ginard B.
      Nature 297:659-664(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1512-1938.
    5. "Cardiac myosin heavy chain isozymic transitions during development and under pathological conditions are regulated at the level of mRNA availability."
      Mahdavi V., Lompre A.M., Chambers A.P., Nadal-Ginard B.
      Eur. Heart J. 5:181-191(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE OF 1872-1938.
      Strain: Wistar.
      Tissue: Heart.

    Entry informationi

    Entry nameiMYH6_RAT
    AccessioniPrimary (citable) accession number: P02563
    Secondary accession number(s): Q63351
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: October 1, 1989
    Last modified: October 1, 2014
    This is version 130 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The cardiac alpha isoform is a 'fast' ATPase myosin, while the beta isoform is a 'slow' ATPase.

    Caution

    Represents a conventional myosin. This protein should not be confused with the unconventional myosin-6 (MYO6).Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3