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Protein

Myosin-6

Gene

Myh6

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Muscle contraction.

Miscellaneous

The cardiac alpha isoform is a 'fast' ATPase myosin, while the beta isoform is a 'slow' ATPase.

Caution

Represents a conventional myosin. This protein should not be confused with the unconventional myosin-6 (MYO6).Curated

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi177 – 184ATP8

GO - Molecular functioni

  • actin filament binding Source: InterPro
  • ATP binding Source: RGD
  • calcium-dependent ATPase activity Source: RGD
  • calmodulin binding Source: UniProtKB-KW
  • identical protein binding Source: IntAct
  • microfilament motor activity Source: RGD
  • microtubule binding Source: InterPro
  • microtubule motor activity Source: InterPro
  • protein heterodimerization activity Source: RGD
  • protein homodimerization activity Source: RGD

GO - Biological processi

  • aging Source: RGD
  • cellular response to 3,3',5-triiodo-L-thyronine Source: RGD
  • microtubule-based movement Source: InterPro
  • muscle contraction Source: RGD
  • response to heat Source: RGD

Keywordsi

Molecular functionActin-binding, Calmodulin-binding, Motor protein, Muscle protein, Myosin
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin-6
Alternative name(s):
Myosin heavy chain 6
Myosin heavy chain, cardiac muscle alpha isoform
Short name:
MyHC-alpha
Gene namesi
Name:Myh6
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi62029 Myh6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Thick filament

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001234041 – 1938Myosin-6Add BLAST1938

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei128N6,N6,N6-trimethyllysineSequence analysis1
Modified residuei378PhosphothreonineCombined sources1
Modified residuei416PhosphoserineCombined sources1
Modified residuei1089PhosphoserineCombined sources1
Modified residuei1138PhosphoserineCombined sources1
Modified residuei1260PhosphotyrosineCombined sources1
Modified residuei1270PhosphoserineCombined sources1
Modified residuei1276PhosphothreonineCombined sources1
Modified residuei1283PhosphothreonineCombined sources1
Modified residuei1308PhosphoserineCombined sources1
Modified residuei1309PhosphotyrosineCombined sources1
Modified residuei1310PhosphothreonineCombined sources1
Modified residuei1511PhosphoserineCombined sources1
Modified residuei1514PhosphothreonineCombined sources1
Modified residuei1680PhosphothreonineCombined sources1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

PaxDbiP02563
PeptideAtlasiP02563
PRIDEiP02563

PTM databases

iPTMnetiP02563
PhosphoSitePlusiP02563

Interactioni

Subunit structurei

Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).

Binary interactionsi

WithEntry#Exp.IntActNotes
itself6EBI-6122328,EBI-6122328

GO - Molecular functioni

  • actin filament binding Source: InterPro
  • calmodulin binding Source: UniProtKB-KW
  • identical protein binding Source: IntAct
  • microtubule binding Source: InterPro
  • protein heterodimerization activity Source: RGD
  • protein homodimerization activity Source: RGD

Protein-protein interaction databases

DIPiDIP-41048N
IntActiP02563, 2 interactors
MINTiP02563
STRINGi10116.ENSRNOP00000023301

Structurei

3D structure databases

ProteinModelPortaliP02563
SMRiP02563
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 80Myosin N-terminal SH3-likePROSITE-ProRule annotationAdd BLAST50
Domaini84 – 779Myosin motorPROSITE-ProRule annotationAdd BLAST696
Domaini782 – 811IQPROSITE-ProRule annotationAdd BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni656 – 678Actin-bindingAdd BLAST23
Regioni758 – 772Actin-bindingAdd BLAST15
Regioni789 – 806Calmodulin-bindingBy similarityAdd BLAST18
Regioni815 – 832Calmodulin-bindingBy similarityAdd BLAST18

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili842 – 1938Sequence analysisAdd BLAST1097

Domaini

The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).Curated

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0161 Eukaryota
COG5022 LUCA
HOGENOMiHOG000173959
HOVERGENiHBG004704
InParanoidiP02563
PhylomeDBiP02563

Family and domain databases

Gene3Di2.30.30.360, 1 hit
3.40.850.10, 3 hits
4.10.270.10, 1 hit
InterProiView protein in InterPro
IPR000048 IQ_motif_EF-hand-BS
IPR036961 Kinesin_motor_dom_sf
IPR001609 Myosin_head_motor_dom
IPR027401 Myosin_IQ_contain_sf
IPR004009 Myosin_N
IPR008989 Myosin_S1_N
IPR002928 Myosin_tail
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00063 Myosin_head, 1 hit
PF02736 Myosin_N, 1 hit
PF01576 Myosin_tail_1, 1 hit
PRINTSiPR00193 MYOSINHEAVY
SMARTiView protein in SMART
SM00242 MYSc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS50096 IQ, 1 hit
PS51456 MYOSIN_MOTOR, 1 hit
PS51844 SH3_LIKE, 1 hit

Sequencei

Sequence statusi: Complete.

P02563-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDAQMADFG AARYLRKSEK ERLEAQTRPF DIRTECFVPD DKEEYVKAKI
60 70 80 90 100
VSREGGKVTA ETENGKTVTV KEDQVMQQNP PKFDKIEDMA MLTFLHEPAV
110 120 130 140 150
LYNLKERYAA WMIYTYSGLF CVTVNPYKWL PVYNAEVVAA YRGKKRSEAP
160 170 180 190 200
PHIFSISDNA YQYMLTDREN QSILITGESG AGKTVNTKRV IQYFASIAAI
210 220 230 240 250
GDRSKKDNPN ANKGTLEDQI IQANPALEAF GNAKTVRNDN SSRFGKFIRI
260 270 280 290 300
HFGATGKLAS ADIETYLLEK SRVIFQLKAE RNYHIFYQIL SNKKPELLDM
310 320 330 340 350
LLVTNNPYDY AFVSQGEVSV ASIDDSEELL ATDSAFDVLG FTAEEKAGVY
360 370 380 390 400
KLTGAIMHYG NMKFKQKQRE EQAEPDGTED ADKSAYLMGL NSADLLKGLC
410 420 430 440 450
HPRVKVGNEY VTKGQSVQQV YYSIGALAKS VYEKMFNWMV TRINATLETK
460 470 480 490 500
QPRQYFIGVL DIAGFEIFDF NSFEQLCINF TNEKLQQFFN HHMFVLEQEE
510 520 530 540 550
YKKEGIEWEF IDFGMDLQAC IDLIEKPMGI MSILEEECMF PKATDMTFKA
560 570 580 590 600
KLYDNHLGKS NNFQKPRNVK GKQEAHFSLV HYAGTVDYNI LGWLEKNKDP
610 620 630 640 650
LNETVVGLYQ KSSLKLMATL FSTYASADTG DSGKGKGGKK KGSSFQTVSA
660 670 680 690 700
LHRENLNKLM TNLRTTHPHF VRCIIPNERK APGVMDNPLV MHQLRCNGVL
710 720 730 740 750
EGIRICRKGF PNRILYGDFR QRYRILNPAA IPEGQFIDSG KGAEKLLGSL
760 770 780 790 800
DIDHNQYKFG HTKVFFKAGL LGLLEEMRDE RLSRIITRIQ AQARGQLMRI
810 820 830 840 850
EFKKMVERRD ALLVIQWNIR AFMGVKNWPW MKLYFKIKPL LKSAETEKEM
860 870 880 890 900
ANMKEEFGRV KDALEKSEAR RKELEEKMVS LLQEKNDLQL QVQAEQDNLA
910 920 930 940 950
DAEERCDQLI KNKIQLEAKV KEMTERLEDE EEMNAELTAK KRKLEDECSE
960 970 980 990 1000
LKKDIDDLEL TLAKVEKEKH ATENKVKNLT EEMAGLDEII AKLTKEKKAL
1010 1020 1030 1040 1050
QEAHQQALDD LQAEEDKVNT LTKSKVKLEQ QVDDLEGSLE QEKKVRMDLE
1060 1070 1080 1090 1100
RAKRKLEGDL KLTQESIMDL ENDKLQLEEK LKKKEFDISQ QNSKIEDEQA
1110 1120 1130 1140 1150
LALQLQKKLK ENQARIEELE EELEAERTAR AKVEKLRSDL TRELEEISER
1160 1170 1180 1190 1200
LEEAGGATSV QIEMNKKREA EFQKMRRDLE EATLQHEATA AALRKKHADS
1210 1220 1230 1240 1250
VAELGEQIDN LQRVKQKLEK EKSEFKLELD DVTSHMEQII KAKANLEKVS
1260 1270 1280 1290 1300
RTLEDQANEY RVKLEEAQRS LNDFTTQRAK LQTENGELAR QLEEKEALIW
1310 1320 1330 1340 1350
QLTRGKLSYT QQMEDLKRQL EEEGKAKNAL AHALQSARHD CDLLREQYEE
1360 1370 1380 1390 1400
EMEAKAELQR VLSKANSEVA QWRTKYETDA IQRTEELEEA KKKLAQRLQD
1410 1420 1430 1440 1450
AEEAVEAVNA KCSSLEKTKH RLQNEIEDLM VDVERSNAAA AALDKKQRNF
1460 1470 1480 1490 1500
DKILAEWKQK YEESQSELES SQKEARSLST ELFKLKNAYE ESLEHLETFK
1510 1520 1530 1540 1550
RENKNLQEEI SDLTEQLGEG GKNVHELEKI RKQLEVEKLE LQSALEEAEA
1560 1570 1580 1590 1600
SLEHEEGKIL RAQLEFNQIK AEIERKLAEK DEEMEQAKRN HLRVVDSLQT
1610 1620 1630 1640 1650
SLDAETRSRN EALRVKKKME GDLNEMEIQL SQANRIASEA QKHLKNAQAH
1660 1670 1680 1690 1700
LKDTQLQLDD AVRANDDLKE NIAIVERRNT LLQAELEELR AVVEQTERSR
1710 1720 1730 1740 1750
KLAEQELIET SERVQLLHSQ NTSLINQKKK MDADLSQLQT EVEEAVQECR
1760 1770 1780 1790 1800
NAEEKAKKAI TDAAMMAEEL KKEQDTSAHL ERMKKNMEQT IKDLQHRLDE
1810 1820 1830 1840 1850
AEQIALKGGK KQLQKLEARV RELENELEAE QKRNAESVKG MRKSERRIKE
1860 1870 1880 1890 1900
LTYQTEEDKK NLVRLQDLVD KLQLKVKAYK RQAEEAEEQA NTNLSKFRKV
1910 1920 1930
QHELDEAEER ADIAESQVNK LRAKSRDIGA KQKMHDEE
Length:1,938
Mass (Da):223,508
Last modified:October 1, 1989 - v2
Checksum:iD7BD33FC2B19E3C2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti13R → AP (PubMed:6585819).Curated1
Sequence conflicti46V → A in AAA41648 (PubMed:6585819).Curated1
Sequence conflicti51 – 52VS → AP in AAA41648 (PubMed:6585819).Curated2
Sequence conflicti87E → Q in AAA41648 (PubMed:6585819).Curated1
Sequence conflicti109Missing in AAA41648 (PubMed:6585819).Curated1
Sequence conflicti1566F → FF (PubMed:7045682).Curated1
Sequence conflicti1575R → S in AAA41653 (PubMed:7045682).Curated1
Sequence conflicti1721N → T (PubMed:7045682).Curated1
Sequence conflicti1852T → N in AAA41653 (PubMed:7045682).Curated1
Sequence conflicti1870D → N in AAA41653 (PubMed:7045682).Curated1
Sequence conflicti1934M → I (PubMed:7045682).Curated1
Sequence conflicti1934M → I (PubMed:6241892).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15938 mRNA Translation: CAA34064.1
K01464 Genomic DNA Translation: AAA41648.1
J00751 mRNA Translation: AAA41653.1
M32697 mRNA Translation: AAA41658.1
PIRiS06005
UniGeneiRn.225886
Rn.54399

Genome annotation databases

UCSCiRGD:62029 rat

Similar proteinsi

Entry informationi

Entry nameiMYH6_RAT
AccessioniPrimary (citable) accession number: P02563
Secondary accession number(s): Q63351
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: October 1, 1989
Last modified: May 23, 2018
This is version 155 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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