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P02563 (MYH6_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Myosin-6
Alternative name(s):
Myosin heavy chain 6
Myosin heavy chain, cardiac muscle alpha isoform
Short name=MyHC-alpha
Gene names
Name:Myh6
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length1938 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Muscle contraction.

Subunit structure

Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).

Subcellular location

Cytoplasmmyofibril. Note: Thick filaments of the myofibrils.

Domain

The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.

Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).

Miscellaneous

The cardiac alpha isoform isa 'fast' ATPase myosin, while the beta isoform isa 'slow' ATPase.

Sequence similarities

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.

Contains 1 IQ domain.

Contains 1 myosin motor domain.

Caution

Represents a conventional myosin. This protein should not be confused with the unconventional myosin-6 (MYO6).

Ontologies

Keywords
   Cellular componentCytoplasm
Thick filament
   DomainCoiled coil
   LigandActin-binding
ATP-binding
Calmodulin-binding
Nucleotide-binding
   Molecular functionMotor protein
Muscle protein
Myosin
   PTMIsopeptide bond
Methylation
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processATP catabolic process

Inferred from mutant phenotype PubMed 15471982. Source: RGD

muscle contraction

Inferred from mutant phenotype PubMed 15020307PubMed 15471982. Source: RGD

response to heat

Inferred from expression pattern PubMed 22285644. Source: RGD

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 9359883. Source: RGD

mitochondrion

Inferred from direct assay PubMed 20533907. Source: RGD

muscle myosin complex

Inferred from direct assay PubMed 3693405. Source: RGD

myofibril

Inferred from direct assay PubMed 10651160. Source: RGD

myosin filament

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from direct assay PubMed 10651160. Source: RGD

calcium-dependent ATPase activity

Inferred from mutant phenotype PubMed 10651160. Source: RGD

identical protein binding

Inferred from physical interaction PubMed 22728135. Source: IntAct

microfilament motor activity

Inferred from mutant phenotype PubMed 15020307. Source: RGD

protein heterodimerization activity

Inferred from direct assay PubMed 3693405. Source: RGD

protein homodimerization activity

Inferred from direct assay PubMed 3693405. Source: RGD

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself6EBI-6122328,EBI-6122328

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 19381938Myosin-6
PRO_0000123404

Regions

Domain84 – 779696Myosin motor
Domain782 – 81130IQ
Nucleotide binding177 – 1848ATP
Region656 – 67823Actin-binding
Region758 – 77215Actin-binding
Region789 – 80618Calmodulin-binding By similarity
Region815 – 83218Calmodulin-binding By similarity
Coiled coil842 – 19381097 Potential

Amino acid modifications

Modified residue1281N6,N6,N6-trimethyllysine Potential
Cross-link205Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Cross-link206Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Cross-link213Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Cross-link1280Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Cross-link1538Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Cross-link1570Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Experimental info

Sequence conflict131R → AP Ref.3
Sequence conflict461V → A in AAA41648. Ref.3
Sequence conflict51 – 522VS → AP in AAA41648. Ref.3
Sequence conflict871E → Q in AAA41648. Ref.3
Sequence conflict1091Missing in AAA41648. Ref.3
Sequence conflict15661F → FF Ref.4
Sequence conflict15751R → S in AAA41653. Ref.4
Sequence conflict17211N → T Ref.4
Sequence conflict18521T → N in AAA41653. Ref.4
Sequence conflict18701D → N in AAA41653. Ref.4
Sequence conflict19341M → I Ref.4
Sequence conflict19341M → I Ref.5

Sequences

Sequence LengthMass (Da)Tools
P02563 [UniParc].

Last modified October 1, 1989. Version 2.
Checksum: D7BD33FC2B19E3C2

FASTA1,938223,508
        10         20         30         40         50         60 
MTDAQMADFG AARYLRKSEK ERLEAQTRPF DIRTECFVPD DKEEYVKAKI VSREGGKVTA 

        70         80         90        100        110        120 
ETENGKTVTV KEDQVMQQNP PKFDKIEDMA MLTFLHEPAV LYNLKERYAA WMIYTYSGLF 

       130        140        150        160        170        180 
CVTVNPYKWL PVYNAEVVAA YRGKKRSEAP PHIFSISDNA YQYMLTDREN QSILITGESG 

       190        200        210        220        230        240 
AGKTVNTKRV IQYFASIAAI GDRSKKDNPN ANKGTLEDQI IQANPALEAF GNAKTVRNDN 

       250        260        270        280        290        300 
SSRFGKFIRI HFGATGKLAS ADIETYLLEK SRVIFQLKAE RNYHIFYQIL SNKKPELLDM 

       310        320        330        340        350        360 
LLVTNNPYDY AFVSQGEVSV ASIDDSEELL ATDSAFDVLG FTAEEKAGVY KLTGAIMHYG 

       370        380        390        400        410        420 
NMKFKQKQRE EQAEPDGTED ADKSAYLMGL NSADLLKGLC HPRVKVGNEY VTKGQSVQQV 

       430        440        450        460        470        480 
YYSIGALAKS VYEKMFNWMV TRINATLETK QPRQYFIGVL DIAGFEIFDF NSFEQLCINF 

       490        500        510        520        530        540 
TNEKLQQFFN HHMFVLEQEE YKKEGIEWEF IDFGMDLQAC IDLIEKPMGI MSILEEECMF 

       550        560        570        580        590        600 
PKATDMTFKA KLYDNHLGKS NNFQKPRNVK GKQEAHFSLV HYAGTVDYNI LGWLEKNKDP 

       610        620        630        640        650        660 
LNETVVGLYQ KSSLKLMATL FSTYASADTG DSGKGKGGKK KGSSFQTVSA LHRENLNKLM 

       670        680        690        700        710        720 
TNLRTTHPHF VRCIIPNERK APGVMDNPLV MHQLRCNGVL EGIRICRKGF PNRILYGDFR 

       730        740        750        760        770        780 
QRYRILNPAA IPEGQFIDSG KGAEKLLGSL DIDHNQYKFG HTKVFFKAGL LGLLEEMRDE 

       790        800        810        820        830        840 
RLSRIITRIQ AQARGQLMRI EFKKMVERRD ALLVIQWNIR AFMGVKNWPW MKLYFKIKPL 

       850        860        870        880        890        900 
LKSAETEKEM ANMKEEFGRV KDALEKSEAR RKELEEKMVS LLQEKNDLQL QVQAEQDNLA 

       910        920        930        940        950        960 
DAEERCDQLI KNKIQLEAKV KEMTERLEDE EEMNAELTAK KRKLEDECSE LKKDIDDLEL 

       970        980        990       1000       1010       1020 
TLAKVEKEKH ATENKVKNLT EEMAGLDEII AKLTKEKKAL QEAHQQALDD LQAEEDKVNT 

      1030       1040       1050       1060       1070       1080 
LTKSKVKLEQ QVDDLEGSLE QEKKVRMDLE RAKRKLEGDL KLTQESIMDL ENDKLQLEEK 

      1090       1100       1110       1120       1130       1140 
LKKKEFDISQ QNSKIEDEQA LALQLQKKLK ENQARIEELE EELEAERTAR AKVEKLRSDL 

      1150       1160       1170       1180       1190       1200 
TRELEEISER LEEAGGATSV QIEMNKKREA EFQKMRRDLE EATLQHEATA AALRKKHADS 

      1210       1220       1230       1240       1250       1260 
VAELGEQIDN LQRVKQKLEK EKSEFKLELD DVTSHMEQII KAKANLEKVS RTLEDQANEY 

      1270       1280       1290       1300       1310       1320 
RVKLEEAQRS LNDFTTQRAK LQTENGELAR QLEEKEALIW QLTRGKLSYT QQMEDLKRQL 

      1330       1340       1350       1360       1370       1380 
EEEGKAKNAL AHALQSARHD CDLLREQYEE EMEAKAELQR VLSKANSEVA QWRTKYETDA 

      1390       1400       1410       1420       1430       1440 
IQRTEELEEA KKKLAQRLQD AEEAVEAVNA KCSSLEKTKH RLQNEIEDLM VDVERSNAAA 

      1450       1460       1470       1480       1490       1500 
AALDKKQRNF DKILAEWKQK YEESQSELES SQKEARSLST ELFKLKNAYE ESLEHLETFK 

      1510       1520       1530       1540       1550       1560 
RENKNLQEEI SDLTEQLGEG GKNVHELEKI RKQLEVEKLE LQSALEEAEA SLEHEEGKIL 

      1570       1580       1590       1600       1610       1620 
RAQLEFNQIK AEIERKLAEK DEEMEQAKRN HLRVVDSLQT SLDAETRSRN EALRVKKKME 

      1630       1640       1650       1660       1670       1680 
GDLNEMEIQL SQANRIASEA QKHLKNAQAH LKDTQLQLDD AVRANDDLKE NIAIVERRNT 

      1690       1700       1710       1720       1730       1740 
LLQAELEELR AVVEQTERSR KLAEQELIET SERVQLLHSQ NTSLINQKKK MDADLSQLQT 

      1750       1760       1770       1780       1790       1800 
EVEEAVQECR NAEEKAKKAI TDAAMMAEEL KKEQDTSAHL ERMKKNMEQT IKDLQHRLDE 

      1810       1820       1830       1840       1850       1860 
AEQIALKGGK KQLQKLEARV RELENELEAE QKRNAESVKG MRKSERRIKE LTYQTEEDKK 

      1870       1880       1890       1900       1910       1920 
NLVRLQDLVD KLQLKVKAYK RQAEEAEEQA NTNLSKFRKV QHELDEAEER ADIAESQVNK 

      1930 
LRAKSRDIGA KQKMHDEE 

« Hide

References

[1]"Complete nucleotide sequence of full length cDNA for rat alpha cardiac myosin heavy chain."
Kraft R., Bravo-Zehnder M., Taylor D., Leinwand L.A.
Nucleic Acids Res. 17:7527-7528(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Heart.
[2]"Full-length rat alpha and beta cardiac myosin heavy chain sequences. Comparisons suggest a molecular basis for functional differences."
McNally E.M., Kraft R., Bravo-Zehnder M., Taylor D., Leinwand L.A.
J. Mol. Biol. 210:665-671(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: DISCUSSION OF SEQUENCE.
[3]"Cardiac alpha- and beta-myosin heavy chain genes are organized in tandem."
Mahdavi V., Chambers A.P., Nadal-Ginard B.
Proc. Natl. Acad. Sci. U.S.A. 81:2626-2630(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-167.
[4]"Molecular characterization of two myosin heavy chain genes expressed in the adult heart."
Mahdavi V., Periasamy M., Nadal-Ginard B.
Nature 297:659-664(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1512-1938.
[5]"Cardiac myosin heavy chain isozymic transitions during development and under pathological conditions are regulated at the level of mRNA availability."
Mahdavi V., Lompre A.M., Chambers A.P., Nadal-Ginard B.
Eur. Heart J. 5:181-191(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 1872-1938.
Strain: Wistar.
Tissue: Heart.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X15938 mRNA. Translation: CAA34064.1.
K01464 Genomic DNA. Translation: AAA41648.1.
J00751 mRNA. Translation: AAA41653.1.
M32697 mRNA. Translation: AAA41658.1.
PIRS06005.
UniGeneRn.54399.

3D structure databases

ProteinModelPortalP02563.
SMRP02563. Positions 839-964.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-41048N.
IntActP02563. 1 interaction.
MINTMINT-348910.
STRING10116.ENSRNOP00000023301.

PTM databases

PhosphoSiteP02563.

Proteomic databases

PaxDbP02563.
PRIDEP02563.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCRGD:62029. rat.

Organism-specific databases

RGD62029. Myh6.

Phylogenomic databases

eggNOGCOG5022.
HOGENOMHOG000173959.
HOVERGENHBG004704.
InParanoidP02563.
PhylomeDBP02563.

Gene expression databases

GenevestigatorP02563.

Family and domain databases

Gene3D4.10.270.10. 1 hit.
InterProIPR000048. IQ_motif_EF-hand-BS.
IPR027401. Myosin-like_IQ_dom.
IPR001609. Myosin_head_motor_dom.
IPR004009. Myosin_N.
IPR002928. Myosin_tail.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00063. Myosin_head. 1 hit.
PF02736. Myosin_N. 1 hit.
PF01576. Myosin_tail_1. 1 hit.
[Graphical view]
PRINTSPR00193. MYOSINHEAVY.
SMARTSM00015. IQ. 2 hits.
SM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS50096. IQ. 1 hit.
PS51456. MYOSIN_MOTOR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMYH6_RAT
AccessionPrimary (citable) accession number: P02563
Secondary accession number(s): Q63351
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: October 1, 1989
Last modified: July 9, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families