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P02563

- MYH6_RAT

UniProt

P02563 - MYH6_RAT

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Protein

Myosin-6

Gene
Myh6
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Muscle contraction.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi177 – 1848ATP

GO - Molecular functioni

  1. ATP binding Source: RGD
  2. calcium-dependent ATPase activity Source: RGD
  3. identical protein binding Source: IntAct
  4. microfilament motor activity Source: RGD
  5. protein heterodimerization activity Source: RGD
  6. protein homodimerization activity Source: RGD

GO - Biological processi

  1. ATP catabolic process Source: RGD
  2. muscle contraction Source: RGD
  3. response to heat Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Muscle protein, Myosin

Keywords - Ligandi

Actin-binding, ATP-binding, Calmodulin-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin-6
Alternative name(s):
Myosin heavy chain 6
Myosin heavy chain, cardiac muscle alpha isoform
Short name:
MyHC-alpha
Gene namesi
Name:Myh6
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi62029. Myh6.

Subcellular locationi

Cytoplasmmyofibril
Note: Thick filaments of the myofibrils.

GO - Cellular componenti

  1. cytoplasm Source: RGD
  2. mitochondrion Source: RGD
  3. muscle myosin complex Source: RGD
  4. myofibril Source: RGD
  5. myosin filament Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Thick filament

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 19381938Myosin-6PRO_0000123404Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei128 – 1281N6,N6,N6-trimethyllysine Reviewed prediction
Cross-linki205 – 205Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Cross-linki206 – 206Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Cross-linki213 – 213Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Cross-linki1280 – 1280Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Cross-linki1538 – 1538Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Cross-linki1570 – 1570Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Keywords - PTMi

Isopeptide bond, Methylation, Ubl conjugation

Proteomic databases

PaxDbiP02563.
PRIDEiP02563.

PTM databases

PhosphoSiteiP02563.

Expressioni

Gene expression databases

GenevestigatoriP02563.

Interactioni

Subunit structurei

Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).

Binary interactionsi

WithEntry#Exp.IntActNotes
itself6EBI-6122328,EBI-6122328

Protein-protein interaction databases

DIPiDIP-41048N.
IntActiP02563. 1 interaction.
MINTiMINT-348910.
STRINGi10116.ENSRNOP00000023301.

Structurei

3D structure databases

ProteinModelPortaliP02563.
SMRiP02563. Positions 839-964.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini84 – 779696Myosin motorAdd
BLAST
Domaini782 – 81130IQAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni656 – 67823Actin-bindingAdd
BLAST
Regioni758 – 77215Actin-bindingAdd
BLAST
Regioni789 – 80618Calmodulin-binding By similarityAdd
BLAST
Regioni815 – 83218Calmodulin-binding By similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili842 – 19381097 Reviewed predictionAdd
BLAST

Domaini

The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).

Sequence similaritiesi

Contains 1 IQ domain.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG5022.
HOGENOMiHOG000173959.
HOVERGENiHBG004704.
InParanoidiP02563.
PhylomeDBiP02563.

Family and domain databases

Gene3Di4.10.270.10. 1 hit.
InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR027401. Myosin-like_IQ_dom.
IPR001609. Myosin_head_motor_dom.
IPR004009. Myosin_N.
IPR002928. Myosin_tail.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00063. Myosin_head. 1 hit.
PF02736. Myosin_N. 1 hit.
PF01576. Myosin_tail_1. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00015. IQ. 2 hits.
SM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50096. IQ. 1 hit.
PS51456. MYOSIN_MOTOR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P02563-1 [UniParc]FASTAAdd to Basket

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MTDAQMADFG AARYLRKSEK ERLEAQTRPF DIRTECFVPD DKEEYVKAKI     50
VSREGGKVTA ETENGKTVTV KEDQVMQQNP PKFDKIEDMA MLTFLHEPAV 100
LYNLKERYAA WMIYTYSGLF CVTVNPYKWL PVYNAEVVAA YRGKKRSEAP 150
PHIFSISDNA YQYMLTDREN QSILITGESG AGKTVNTKRV IQYFASIAAI 200
GDRSKKDNPN ANKGTLEDQI IQANPALEAF GNAKTVRNDN SSRFGKFIRI 250
HFGATGKLAS ADIETYLLEK SRVIFQLKAE RNYHIFYQIL SNKKPELLDM 300
LLVTNNPYDY AFVSQGEVSV ASIDDSEELL ATDSAFDVLG FTAEEKAGVY 350
KLTGAIMHYG NMKFKQKQRE EQAEPDGTED ADKSAYLMGL NSADLLKGLC 400
HPRVKVGNEY VTKGQSVQQV YYSIGALAKS VYEKMFNWMV TRINATLETK 450
QPRQYFIGVL DIAGFEIFDF NSFEQLCINF TNEKLQQFFN HHMFVLEQEE 500
YKKEGIEWEF IDFGMDLQAC IDLIEKPMGI MSILEEECMF PKATDMTFKA 550
KLYDNHLGKS NNFQKPRNVK GKQEAHFSLV HYAGTVDYNI LGWLEKNKDP 600
LNETVVGLYQ KSSLKLMATL FSTYASADTG DSGKGKGGKK KGSSFQTVSA 650
LHRENLNKLM TNLRTTHPHF VRCIIPNERK APGVMDNPLV MHQLRCNGVL 700
EGIRICRKGF PNRILYGDFR QRYRILNPAA IPEGQFIDSG KGAEKLLGSL 750
DIDHNQYKFG HTKVFFKAGL LGLLEEMRDE RLSRIITRIQ AQARGQLMRI 800
EFKKMVERRD ALLVIQWNIR AFMGVKNWPW MKLYFKIKPL LKSAETEKEM 850
ANMKEEFGRV KDALEKSEAR RKELEEKMVS LLQEKNDLQL QVQAEQDNLA 900
DAEERCDQLI KNKIQLEAKV KEMTERLEDE EEMNAELTAK KRKLEDECSE 950
LKKDIDDLEL TLAKVEKEKH ATENKVKNLT EEMAGLDEII AKLTKEKKAL 1000
QEAHQQALDD LQAEEDKVNT LTKSKVKLEQ QVDDLEGSLE QEKKVRMDLE 1050
RAKRKLEGDL KLTQESIMDL ENDKLQLEEK LKKKEFDISQ QNSKIEDEQA 1100
LALQLQKKLK ENQARIEELE EELEAERTAR AKVEKLRSDL TRELEEISER 1150
LEEAGGATSV QIEMNKKREA EFQKMRRDLE EATLQHEATA AALRKKHADS 1200
VAELGEQIDN LQRVKQKLEK EKSEFKLELD DVTSHMEQII KAKANLEKVS 1250
RTLEDQANEY RVKLEEAQRS LNDFTTQRAK LQTENGELAR QLEEKEALIW 1300
QLTRGKLSYT QQMEDLKRQL EEEGKAKNAL AHALQSARHD CDLLREQYEE 1350
EMEAKAELQR VLSKANSEVA QWRTKYETDA IQRTEELEEA KKKLAQRLQD 1400
AEEAVEAVNA KCSSLEKTKH RLQNEIEDLM VDVERSNAAA AALDKKQRNF 1450
DKILAEWKQK YEESQSELES SQKEARSLST ELFKLKNAYE ESLEHLETFK 1500
RENKNLQEEI SDLTEQLGEG GKNVHELEKI RKQLEVEKLE LQSALEEAEA 1550
SLEHEEGKIL RAQLEFNQIK AEIERKLAEK DEEMEQAKRN HLRVVDSLQT 1600
SLDAETRSRN EALRVKKKME GDLNEMEIQL SQANRIASEA QKHLKNAQAH 1650
LKDTQLQLDD AVRANDDLKE NIAIVERRNT LLQAELEELR AVVEQTERSR 1700
KLAEQELIET SERVQLLHSQ NTSLINQKKK MDADLSQLQT EVEEAVQECR 1750
NAEEKAKKAI TDAAMMAEEL KKEQDTSAHL ERMKKNMEQT IKDLQHRLDE 1800
AEQIALKGGK KQLQKLEARV RELENELEAE QKRNAESVKG MRKSERRIKE 1850
LTYQTEEDKK NLVRLQDLVD KLQLKVKAYK RQAEEAEEQA NTNLSKFRKV 1900
QHELDEAEER ADIAESQVNK LRAKSRDIGA KQKMHDEE 1938
Length:1,938
Mass (Da):223,508
Last modified:October 1, 1989 - v2
Checksum:iD7BD33FC2B19E3C2
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti13 – 131R → AP1 Publication
Sequence conflicti46 – 461V → A in AAA41648. 1 Publication
Sequence conflicti51 – 522VS → AP in AAA41648. 1 Publication
Sequence conflicti87 – 871E → Q in AAA41648. 1 Publication
Sequence conflicti109 – 1091Missing in AAA41648. 1 Publication
Sequence conflicti1566 – 15661F → FF1 Publication
Sequence conflicti1575 – 15751R → S in AAA41653. 1 Publication
Sequence conflicti1721 – 17211N → T1 Publication
Sequence conflicti1852 – 18521T → N in AAA41653. 1 Publication
Sequence conflicti1870 – 18701D → N in AAA41653. 1 Publication
Sequence conflicti1934 – 19341M → I1 Publication
Sequence conflicti1934 – 19341M → I1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X15938 mRNA. Translation: CAA34064.1.
K01464 Genomic DNA. Translation: AAA41648.1.
J00751 mRNA. Translation: AAA41653.1.
M32697 mRNA. Translation: AAA41658.1.
PIRiS06005.
UniGeneiRn.54399.

Genome annotation databases

UCSCiRGD:62029. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X15938 mRNA. Translation: CAA34064.1 .
K01464 Genomic DNA. Translation: AAA41648.1 .
J00751 mRNA. Translation: AAA41653.1 .
M32697 mRNA. Translation: AAA41658.1 .
PIRi S06005.
UniGenei Rn.54399.

3D structure databases

ProteinModelPortali P02563.
SMRi P02563. Positions 839-964.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-41048N.
IntActi P02563. 1 interaction.
MINTi MINT-348910.
STRINGi 10116.ENSRNOP00000023301.

PTM databases

PhosphoSitei P02563.

Proteomic databases

PaxDbi P02563.
PRIDEi P02563.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:62029. rat.

Organism-specific databases

RGDi 62029. Myh6.

Phylogenomic databases

eggNOGi COG5022.
HOGENOMi HOG000173959.
HOVERGENi HBG004704.
InParanoidi P02563.
PhylomeDBi P02563.

Gene expression databases

Genevestigatori P02563.

Family and domain databases

Gene3Di 4.10.270.10. 1 hit.
InterProi IPR000048. IQ_motif_EF-hand-BS.
IPR027401. Myosin-like_IQ_dom.
IPR001609. Myosin_head_motor_dom.
IPR004009. Myosin_N.
IPR002928. Myosin_tail.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00063. Myosin_head. 1 hit.
PF02736. Myosin_N. 1 hit.
PF01576. Myosin_tail_1. 1 hit.
[Graphical view ]
PRINTSi PR00193. MYOSINHEAVY.
SMARTi SM00015. IQ. 2 hits.
SM00242. MYSc. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS50096. IQ. 1 hit.
PS51456. MYOSIN_MOTOR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete nucleotide sequence of full length cDNA for rat alpha cardiac myosin heavy chain."
    Kraft R., Bravo-Zehnder M., Taylor D., Leinwand L.A.
    Nucleic Acids Res. 17:7527-7528(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Heart.
  2. "Full-length rat alpha and beta cardiac myosin heavy chain sequences. Comparisons suggest a molecular basis for functional differences."
    McNally E.M., Kraft R., Bravo-Zehnder M., Taylor D., Leinwand L.A.
    J. Mol. Biol. 210:665-671(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISCUSSION OF SEQUENCE.
  3. "Cardiac alpha- and beta-myosin heavy chain genes are organized in tandem."
    Mahdavi V., Chambers A.P., Nadal-Ginard B.
    Proc. Natl. Acad. Sci. U.S.A. 81:2626-2630(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-167.
  4. "Molecular characterization of two myosin heavy chain genes expressed in the adult heart."
    Mahdavi V., Periasamy M., Nadal-Ginard B.
    Nature 297:659-664(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1512-1938.
  5. "Cardiac myosin heavy chain isozymic transitions during development and under pathological conditions are regulated at the level of mRNA availability."
    Mahdavi V., Lompre A.M., Chambers A.P., Nadal-Ginard B.
    Eur. Heart J. 5:181-191(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 1872-1938.
    Strain: Wistar.
    Tissue: Heart.

Entry informationi

Entry nameiMYH6_RAT
AccessioniPrimary (citable) accession number: P02563
Secondary accession number(s): Q63351
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: October 1, 1989
Last modified: July 9, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The cardiac alpha isoform is a 'fast' ATPase myosin, while the beta isoform is a 'slow' ATPase.

Caution

Represents a conventional myosin. This protein should not be confused with the unconventional myosin-6 (MYO6).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi