Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P02563

- MYH6_RAT

UniProt

P02563 - MYH6_RAT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Myosin-6

Gene

Myh6

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Muscle contraction.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi177 – 1848ATP

GO - Molecular functioni

  1. ATP binding Source: RGD
  2. calcium-dependent ATPase activity Source: RGD
  3. identical protein binding Source: IntAct
  4. microfilament motor activity Source: RGD
  5. protein heterodimerization activity Source: RGD
  6. protein homodimerization activity Source: RGD

GO - Biological processi

  1. ATP catabolic process Source: RGD
  2. muscle contraction Source: RGD
  3. response to heat Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Muscle protein, Myosin

Keywords - Ligandi

Actin-binding, ATP-binding, Calmodulin-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin-6
Alternative name(s):
Myosin heavy chain 6
Myosin heavy chain, cardiac muscle alpha isoform
Short name:
MyHC-alpha
Gene namesi
Name:Myh6
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi62029. Myh6.

Subcellular locationi

Cytoplasmmyofibril
Note: Thick filaments of the myofibrils.

GO - Cellular componenti

  1. cytoplasm Source: RGD
  2. mitochondrion Source: RGD
  3. muscle myosin complex Source: RGD
  4. myofibril Source: RGD
  5. myosin filament Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Thick filament

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 19381938Myosin-6PRO_0000123404Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei128 – 1281N6,N6,N6-trimethyllysineSequence Analysis
Cross-linki205 – 205Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki206 – 206Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki213 – 213Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki1280 – 1280Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki1538 – 1538Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki1570 – 1570Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Keywords - PTMi

Isopeptide bond, Methylation, Ubl conjugation

Proteomic databases

PaxDbiP02563.
PRIDEiP02563.

PTM databases

PhosphoSiteiP02563.

Expressioni

Gene expression databases

GenevestigatoriP02563.

Interactioni

Subunit structurei

Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).

Binary interactionsi

WithEntry#Exp.IntActNotes
itself6EBI-6122328,EBI-6122328

Protein-protein interaction databases

DIPiDIP-41048N.
IntActiP02563. 1 interaction.
MINTiMINT-348910.
STRINGi10116.ENSRNOP00000023301.

Structurei

3D structure databases

ProteinModelPortaliP02563.
SMRiP02563. Positions 839-964.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini84 – 779696Myosin motorAdd
BLAST
Domaini782 – 81130IQPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni656 – 67823Actin-bindingAdd
BLAST
Regioni758 – 77215Actin-bindingAdd
BLAST
Regioni789 – 80618Calmodulin-bindingBy similarityAdd
BLAST
Regioni815 – 83218Calmodulin-bindingBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili842 – 19381097Sequence AnalysisAdd
BLAST

Domaini

The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).

Sequence similaritiesi

Contains 1 IQ domain.PROSITE-ProRule annotation
Contains 1 myosin motor domain.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG5022.
HOGENOMiHOG000173959.
HOVERGENiHBG004704.
InParanoidiP02563.
PhylomeDBiP02563.

Family and domain databases

Gene3Di4.10.270.10. 1 hit.
InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR027401. Myosin-like_IQ_dom.
IPR001609. Myosin_head_motor_dom.
IPR004009. Myosin_N.
IPR002928. Myosin_tail.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00063. Myosin_head. 1 hit.
PF02736. Myosin_N. 1 hit.
PF01576. Myosin_tail_1. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00015. IQ. 2 hits.
SM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50096. IQ. 1 hit.
PS51456. MYOSIN_MOTOR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P02563-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTDAQMADFG AARYLRKSEK ERLEAQTRPF DIRTECFVPD DKEEYVKAKI
60 70 80 90 100
VSREGGKVTA ETENGKTVTV KEDQVMQQNP PKFDKIEDMA MLTFLHEPAV
110 120 130 140 150
LYNLKERYAA WMIYTYSGLF CVTVNPYKWL PVYNAEVVAA YRGKKRSEAP
160 170 180 190 200
PHIFSISDNA YQYMLTDREN QSILITGESG AGKTVNTKRV IQYFASIAAI
210 220 230 240 250
GDRSKKDNPN ANKGTLEDQI IQANPALEAF GNAKTVRNDN SSRFGKFIRI
260 270 280 290 300
HFGATGKLAS ADIETYLLEK SRVIFQLKAE RNYHIFYQIL SNKKPELLDM
310 320 330 340 350
LLVTNNPYDY AFVSQGEVSV ASIDDSEELL ATDSAFDVLG FTAEEKAGVY
360 370 380 390 400
KLTGAIMHYG NMKFKQKQRE EQAEPDGTED ADKSAYLMGL NSADLLKGLC
410 420 430 440 450
HPRVKVGNEY VTKGQSVQQV YYSIGALAKS VYEKMFNWMV TRINATLETK
460 470 480 490 500
QPRQYFIGVL DIAGFEIFDF NSFEQLCINF TNEKLQQFFN HHMFVLEQEE
510 520 530 540 550
YKKEGIEWEF IDFGMDLQAC IDLIEKPMGI MSILEEECMF PKATDMTFKA
560 570 580 590 600
KLYDNHLGKS NNFQKPRNVK GKQEAHFSLV HYAGTVDYNI LGWLEKNKDP
610 620 630 640 650
LNETVVGLYQ KSSLKLMATL FSTYASADTG DSGKGKGGKK KGSSFQTVSA
660 670 680 690 700
LHRENLNKLM TNLRTTHPHF VRCIIPNERK APGVMDNPLV MHQLRCNGVL
710 720 730 740 750
EGIRICRKGF PNRILYGDFR QRYRILNPAA IPEGQFIDSG KGAEKLLGSL
760 770 780 790 800
DIDHNQYKFG HTKVFFKAGL LGLLEEMRDE RLSRIITRIQ AQARGQLMRI
810 820 830 840 850
EFKKMVERRD ALLVIQWNIR AFMGVKNWPW MKLYFKIKPL LKSAETEKEM
860 870 880 890 900
ANMKEEFGRV KDALEKSEAR RKELEEKMVS LLQEKNDLQL QVQAEQDNLA
910 920 930 940 950
DAEERCDQLI KNKIQLEAKV KEMTERLEDE EEMNAELTAK KRKLEDECSE
960 970 980 990 1000
LKKDIDDLEL TLAKVEKEKH ATENKVKNLT EEMAGLDEII AKLTKEKKAL
1010 1020 1030 1040 1050
QEAHQQALDD LQAEEDKVNT LTKSKVKLEQ QVDDLEGSLE QEKKVRMDLE
1060 1070 1080 1090 1100
RAKRKLEGDL KLTQESIMDL ENDKLQLEEK LKKKEFDISQ QNSKIEDEQA
1110 1120 1130 1140 1150
LALQLQKKLK ENQARIEELE EELEAERTAR AKVEKLRSDL TRELEEISER
1160 1170 1180 1190 1200
LEEAGGATSV QIEMNKKREA EFQKMRRDLE EATLQHEATA AALRKKHADS
1210 1220 1230 1240 1250
VAELGEQIDN LQRVKQKLEK EKSEFKLELD DVTSHMEQII KAKANLEKVS
1260 1270 1280 1290 1300
RTLEDQANEY RVKLEEAQRS LNDFTTQRAK LQTENGELAR QLEEKEALIW
1310 1320 1330 1340 1350
QLTRGKLSYT QQMEDLKRQL EEEGKAKNAL AHALQSARHD CDLLREQYEE
1360 1370 1380 1390 1400
EMEAKAELQR VLSKANSEVA QWRTKYETDA IQRTEELEEA KKKLAQRLQD
1410 1420 1430 1440 1450
AEEAVEAVNA KCSSLEKTKH RLQNEIEDLM VDVERSNAAA AALDKKQRNF
1460 1470 1480 1490 1500
DKILAEWKQK YEESQSELES SQKEARSLST ELFKLKNAYE ESLEHLETFK
1510 1520 1530 1540 1550
RENKNLQEEI SDLTEQLGEG GKNVHELEKI RKQLEVEKLE LQSALEEAEA
1560 1570 1580 1590 1600
SLEHEEGKIL RAQLEFNQIK AEIERKLAEK DEEMEQAKRN HLRVVDSLQT
1610 1620 1630 1640 1650
SLDAETRSRN EALRVKKKME GDLNEMEIQL SQANRIASEA QKHLKNAQAH
1660 1670 1680 1690 1700
LKDTQLQLDD AVRANDDLKE NIAIVERRNT LLQAELEELR AVVEQTERSR
1710 1720 1730 1740 1750
KLAEQELIET SERVQLLHSQ NTSLINQKKK MDADLSQLQT EVEEAVQECR
1760 1770 1780 1790 1800
NAEEKAKKAI TDAAMMAEEL KKEQDTSAHL ERMKKNMEQT IKDLQHRLDE
1810 1820 1830 1840 1850
AEQIALKGGK KQLQKLEARV RELENELEAE QKRNAESVKG MRKSERRIKE
1860 1870 1880 1890 1900
LTYQTEEDKK NLVRLQDLVD KLQLKVKAYK RQAEEAEEQA NTNLSKFRKV
1910 1920 1930
QHELDEAEER ADIAESQVNK LRAKSRDIGA KQKMHDEE
Length:1,938
Mass (Da):223,508
Last modified:October 1, 1989 - v2
Checksum:iD7BD33FC2B19E3C2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti13 – 131R → AP(PubMed:6585819)Curated
Sequence conflicti46 – 461V → A in AAA41648. (PubMed:6585819)Curated
Sequence conflicti51 – 522VS → AP in AAA41648. (PubMed:6585819)Curated
Sequence conflicti87 – 871E → Q in AAA41648. (PubMed:6585819)Curated
Sequence conflicti109 – 1091Missing in AAA41648. (PubMed:6585819)Curated
Sequence conflicti1566 – 15661F → FF(PubMed:7045682)Curated
Sequence conflicti1575 – 15751R → S in AAA41653. (PubMed:7045682)Curated
Sequence conflicti1721 – 17211N → T(PubMed:7045682)Curated
Sequence conflicti1852 – 18521T → N in AAA41653. (PubMed:7045682)Curated
Sequence conflicti1870 – 18701D → N in AAA41653. (PubMed:7045682)Curated
Sequence conflicti1934 – 19341M → I(PubMed:7045682)Curated
Sequence conflicti1934 – 19341M → I(PubMed:6241892)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X15938 mRNA. Translation: CAA34064.1.
K01464 Genomic DNA. Translation: AAA41648.1.
J00751 mRNA. Translation: AAA41653.1.
M32697 mRNA. Translation: AAA41658.1.
PIRiS06005.
UniGeneiRn.54399.

Genome annotation databases

UCSCiRGD:62029. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X15938 mRNA. Translation: CAA34064.1 .
K01464 Genomic DNA. Translation: AAA41648.1 .
J00751 mRNA. Translation: AAA41653.1 .
M32697 mRNA. Translation: AAA41658.1 .
PIRi S06005.
UniGenei Rn.54399.

3D structure databases

ProteinModelPortali P02563.
SMRi P02563. Positions 839-964.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-41048N.
IntActi P02563. 1 interaction.
MINTi MINT-348910.
STRINGi 10116.ENSRNOP00000023301.

PTM databases

PhosphoSitei P02563.

Proteomic databases

PaxDbi P02563.
PRIDEi P02563.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:62029. rat.

Organism-specific databases

RGDi 62029. Myh6.

Phylogenomic databases

eggNOGi COG5022.
HOGENOMi HOG000173959.
HOVERGENi HBG004704.
InParanoidi P02563.
PhylomeDBi P02563.

Gene expression databases

Genevestigatori P02563.

Family and domain databases

Gene3Di 4.10.270.10. 1 hit.
InterProi IPR000048. IQ_motif_EF-hand-BS.
IPR027401. Myosin-like_IQ_dom.
IPR001609. Myosin_head_motor_dom.
IPR004009. Myosin_N.
IPR002928. Myosin_tail.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00063. Myosin_head. 1 hit.
PF02736. Myosin_N. 1 hit.
PF01576. Myosin_tail_1. 1 hit.
[Graphical view ]
PRINTSi PR00193. MYOSINHEAVY.
SMARTi SM00015. IQ. 2 hits.
SM00242. MYSc. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS50096. IQ. 1 hit.
PS51456. MYOSIN_MOTOR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete nucleotide sequence of full length cDNA for rat alpha cardiac myosin heavy chain."
    Kraft R., Bravo-Zehnder M., Taylor D., Leinwand L.A.
    Nucleic Acids Res. 17:7527-7528(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Heart.
  2. "Full-length rat alpha and beta cardiac myosin heavy chain sequences. Comparisons suggest a molecular basis for functional differences."
    McNally E.M., Kraft R., Bravo-Zehnder M., Taylor D., Leinwand L.A.
    J. Mol. Biol. 210:665-671(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISCUSSION OF SEQUENCE.
  3. "Cardiac alpha- and beta-myosin heavy chain genes are organized in tandem."
    Mahdavi V., Chambers A.P., Nadal-Ginard B.
    Proc. Natl. Acad. Sci. U.S.A. 81:2626-2630(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-167.
  4. "Molecular characterization of two myosin heavy chain genes expressed in the adult heart."
    Mahdavi V., Periasamy M., Nadal-Ginard B.
    Nature 297:659-664(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1512-1938.
  5. "Cardiac myosin heavy chain isozymic transitions during development and under pathological conditions are regulated at the level of mRNA availability."
    Mahdavi V., Lompre A.M., Chambers A.P., Nadal-Ginard B.
    Eur. Heart J. 5:181-191(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 1872-1938.
    Strain: Wistar.
    Tissue: Heart.

Entry informationi

Entry nameiMYH6_RAT
AccessioniPrimary (citable) accession number: P02563
Secondary accession number(s): Q63351
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: October 1, 1989
Last modified: October 1, 2014
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The cardiac alpha isoform is a 'fast' ATPase myosin, while the beta isoform is a 'slow' ATPase.

Caution

Represents a conventional myosin. This protein should not be confused with the unconventional myosin-6 (MYO6).Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3