P02561 (TPM4_HORSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tropomyosin alpha-4 chain

Alternative name(s):
Platelet beta tropomyosin
Tropomyosin-4

Gene names

Name:

TPM4

Organism

Equus caballus (Horse) [Reference proteome]

Taxonomic identifier

9796 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Perissodactyla › Equidae › Equus › Equus

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. Binds calcium.
Subunit structure	Heterodimer of an alpha and a beta chain.
Subcellular location	Cytoplasm › cytoskeleton.
Domain	The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.
Miscellaneous	This protein can span only six actin monomers.
Sequence similarities	Belongs to the tropomyosin family.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Domain	Coiled coil
Ligand	Actin-binding Calcium Metal-binding
Molecular function	Muscle protein
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 248	247	Tropomyosin alpha-4 chain		PRO_0000205634
Regions
	Coiled coil	2 – 248	247	By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	177	1	N6-acetyllysine By similarity
	Modified residue	215	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P02561 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 5D5CDF6BF76BB2A8	FASTA	248	28,523
	10 20 30 40 50 60 MAGLNSLEAV KRKIQALQQQ ADEAEDRAQG LQRELDGERE RREKAEGDVA ALNRRIQLVE 70 80 90 100 110 120 EELDRAQERL ATALQKLEEA EKAADESERG MKVIENRAMK DEEKMEIQEM QLKEAKHIAE 130 140 150 160 170 180 EADRKYEEVA RKLVILEGEL ERAEERAEVS ELKCGDLEEE LKNVTNNLKS LEAASEKYSE 190 200 210 220 230 240 KEDKYEEEIK LLSDKLKEAE TRAEFAERTV AKLEKTIDDL EEKLAQAKEE NVGLHQTLDQ TLDELNCI

References

[1]	"Amino acid sequence of equine platelet tropomyosin. Correlation with interaction properties." Lewis W.G., Cote G.P., Mak A.S., Smillie L.B. FEBS Lett. 156:269-273(1983) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-248.

Cross-references

Sequence databases
PIR	TMHOBP. A02984.
3D structure databases
ProteinModelPortal	P02561.
SMR	P02561. Positions 5-248.
ModBase	Search...
Protein-protein interaction databases
STRING	9796.ENSECAP00000019820.
Proteomic databases
PRIDE	P02561.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	NOG304012.
HOGENOM	HOG000231522.
HOVERGEN	HBG107404.
InParanoid	P02561.
OrthoDB	EOG4TXBSM.
Family and domain databases
InterPro	IPR000533. Tropomyosin. [Graphical view]
Pfam	PF00261. Tropomyosin. 1 hit. [Graphical view]
PRINTS	PR00194. TROPOMYOSIN.
PROSITE	PS00326. TROPOMYOSIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPM4_HORSE

Accession

Primary (citable) accession number: P02561

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program