Tubulin beta chain - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P02557 (TBB_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 120. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Tubulin beta chain

Alternative name(s):
Beta-tubulin

Gene names

Name:	TUB2
Ordered Locus Names:	YFL037W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	457 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
Subunit structure	Dimer of alpha and beta chains.
Subcellular location	Cytoplasm › cytoskeleton.
Miscellaneous	Rhizoxin, an antibiotic that exhibits potent anti-mitotic activity against most eukaryotic cells except for yeasts, binds to beta tubulin.
Sequence similarities	Belongs to the tubulin family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Cellular component	Cytoplasm Cytoskeleton Microtubule
Ligand	GTP-binding Nucleotide-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	homologous chromosome segregation Traceable author statement. Source: SGD mitotic sister chromatid segregation Traceable author statement. Source: SGD nuclear migration involved in conjugation with cellular fusion Traceable author statement. Source: SGD protein polymerization Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasmic microtubule Traceable author statement. Source: SGD kinetochore microtubule Traceable author statement. Source: SGD nuclear microtubule Traceable author statement. Source: SGD polar microtubule Traceable author statement. Source: SGD spindle pole body Inferred from direct assay. Source: SGD
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct structural constituent of cytoskeleton Traceable author statement. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RBL2	P48606	2	EBI-18986,EBI-18991

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 457

457

Tubulin beta chain

PRO_0000048443

Regions

Nucleotide binding

140 – 146

GTP Potential

Amino acid modifications

Modified residue

278

Phosphoserine Ref.6

Modified residue

280

Phosphoserine Ref.6

Experimental info

Mutagenesis

100

V → N: Becomes sensitive to rhizoxin.

Sequence conflict

T → A in CAA24603. Ref.1

Sequence conflict

C → Y in CAA24603. Ref.1

Sequence conflict

G → W in CAA24603. Ref.1

Sequence conflict

152

I → F in CAA24603. Ref.1

Sequence conflict

156

R → K in CAA24603. Ref.1

Sequence conflict

159

F → L in CAA24603. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P02557 [UniParc].

Last modified November 1, 1995. Version 2.
Checksum: 68EBEA7D7A5B8EA1
Show »

FASTA

457

50,923

        10         20         30         40         50         60 
MREIIHISTG QCGNQIGAAF WETICGEHGL DFNGTYHGHD DIQKERLNVY FNEASSGKWV 

        70         80         90        100        110        120 
PRSINVDLEP GTIDAVRNSA IGNLFRPDNY IFGQSSAGNV WAKGHYTEGA ELVDSVMDVI 

       130        140        150        160        170        180 
RREAEGCDSL QGFQITHSLG GGTGSGMGTL LISKIREEFP DRMMATFSVL PSPKTSDTVV 

       190        200        210        220        230        240 
EPYNATLSVH QLVEHSDETF CIDNEALYDI CQRTLKLNQP SYGDLNNLVS SVMSGVTTSL 

       250        260        270        280        290        300 
RYPGQLNSDL RKLAVNLVPF PRLHFFMVGY APLTAIGSQS FRSLTVPELT QQMFDAKNMM 

       310        320        330        340        350        360 
AAADPRNGRY LTVAAFFRGK VSVKEVEDEM HKVQSKNSDY FVEWIPNNVQ TAVCSVAPQG 

       370        380        390        400        410        420 
LDMAATFIAN STSIQELFKR VGDQFSAMFK RKAFLHWYTS EGMDELEFSE AESNMNDLVS 

       430        440        450 
EYQQYQEATV EDDEEVDENG DFGAPQNQDE PITENFE

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of the beta-tubulin gene from yeast and demonstration of its essential function in vivo." Neff N.F., Thomas J.H., Grisafi P., Botstein D. Cell 33:211-219(1983) [PubMed: 6380751] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"A yeast gene encoding a protein homologous to the human c-has/bas proto-oncogene product." Gallwitz D., Donath C., Sander C. Nature 306:704-707(1983) [PubMed: 6318115] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-8.
[5]	"Molecular basis for determining the sensitivity of eucaryotes to the antimitotic drug rhizoxin." Takahashi M., Matsumoto S., Iwasaki S., Yahara I. Mol. Gen. Genet. 222:169-175(1990) [PubMed: 2274023] [Abstract] Cited for: ANTIBIOTIC RESISTANCE TO RHIZOXIN.
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-278 AND SER-280, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	V01296 Genomic DNA. Translation: CAA24603.1. D50617 Genomic DNA. Translation: BAA09202.1. X00209 Genomic DNA. Translation: CAA25035.1. BK006940 Genomic DNA. Translation: DAA12403.1.
PIR	UBBYB. S56217.
RefSeq	NP_116616.1. NM_001179929.1.
3D structure databases
ProteinModelPortal	P02557.
SMR	P02557. Positions 2-427.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2340N.
IntAct	P02557. 268 interactions.
MINT	MINT-545919.
STRING	P02557.
Proteomic databases
PeptideAtlas	P02557.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YFL037W; YFL037W; YFL037W.
GeneID	850506.
KEGG	sce:YFL037W.
NMPDR	fig\|4932.3.peg.2241.
Organism-specific databases
CYGD	YFL037w.
SGD	S000001857. TUB2.
Phylogenomic databases
eggNOG	fuNOG05200.
GeneTree	EFGT00050000001868.
HOGENOM	HBG750007.
OMA	ACIFRGP.
OrthoDB	EOG4B2X5K.
Gene expression databases
ArrayExpress	P02557.
Genevestigator	P02557.
GermOnline	YFL037W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013838. Beta-tubulin_BS. IPR002453. Beta_tubulin. IPR008280. Tub_FtsZ_C. IPR000217. Tubulin. IPR018316. Tubulin/FtsZ_2-layer-sand-dom. IPR023123. Tubulin_C. IPR017975. Tubulin_CS. IPR003008. Tubulin_FtsZ_GTPase. [Graphical view]
Gene3D	G3DSA:3.30.1330.20. Tubulin/FtsZ_2-layer-sand-dom. 1 hit. G3DSA:1.10.287.600. Tubulin_C. 1 hit. G3DSA:3.40.50.1440. Tubulin_FtsZ. 1 hit.
KO	K07375.
Pfam	PF00091. Tubulin. 1 hit. PF03953. Tubulin_C. 1 hit. [Graphical view]
PRINTS	PR01163. BETATUBULIN. PR01161. TUBULIN.
SMART	SM00864. Tubulin. 1 hit. SM00865. Tubulin_C. 1 hit. [Graphical view]
SUPFAM	SSF55307. Tub_FtsZ_C. 1 hit. SSF52490. Tubulin_FtsZ. 1 hit.
PROSITE	PS00227. TUBULIN. 1 hit. PS00228. TUBULIN_B_AUTOREG. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	966212.

Entry information

Entry name

TBB_YEAST

Accession

Primary (citable) accession number: P02557
Secondary accession number(s): D6VTJ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	November 1, 1995
Last modified:	December 14, 2011
This is version 120 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VI

Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents