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Protein

Neurofilament light polypeptide

Gene

NEFL

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-BTA-438066. Unblocking of NMDA receptor, glutamate binding and activation.
R-BTA-442729. CREB phosphorylation through the activation of CaMKII.
R-BTA-442982. Ras activation uopn Ca2+ infux through NMDA receptor.
R-BTA-5673001. RAF/MAP kinase cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
Neurofilament light polypeptide
Short name:
NF-L
Alternative name(s):
68 kDa neurofilament protein
Micro glutamic acid-rich protein
Neurofilament triplet L protein
Gene namesi
Name:NEFL
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 8

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Intermediate filament

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000637862 – 555Neurofilament light polypeptideAdd BLAST554

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserine1 Publication1
Modified residuei23Asymmetric dimethylarginine; alternateBy similarity1
Modified residuei23Omega-N-methylarginine; alternateBy similarity1
Modified residuei30Omega-N-methylarginineBy similarity1
Modified residuei43PhosphotyrosineBy similarity1
Modified residuei56Phosphoserine1 Publication1
Modified residuei67Phosphoserine1 Publication1
Modified residuei103PhosphoserineBy similarity1
Modified residuei473Phosphoserine1 Publication1
Modified residuei532PhosphothreonineBy similarity1

Post-translational modificationi

O-glycosylated.By similarity
Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.By similarity
Ubiquitinated in the presence of TRIM2 and UBE2D1.By similarity

Keywords - PTMi

Acetylation, Glycoprotein, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP02548.
PeptideAtlasiP02548.
PRIDEiP02548.

PTM databases

iPTMnetiP02548.

Expressioni

Gene expression databases

BgeeiENSBTAG00000021949.

Interactioni

Subunit structurei

Interacts with ARHGEF28. Interacts with TRIM2.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiP02548. 1 interactor.
STRINGi9913.ENSBTAP00000029264.

Structurei

3D structure databases

ProteinModelPortaliP02548.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 93HeadAdd BLAST92
Regioni94 – 397RodAdd BLAST304
Regioni94 – 125Coil 1AAdd BLAST32
Regioni126 – 138Linker 1Add BLAST13
Regioni139 – 234Coil 1BAdd BLAST96
Regioni235 – 253Linker 12Add BLAST19
Regioni254 – 272Coil 2AAdd BLAST19
Regioni273 – 281Linker 29
Regioni282 – 397Coil 2BAdd BLAST116
Regioni398 – 555TailAdd BLAST158
Regioni398 – 444Tail, subdomain AAdd BLAST47
Regioni445 – 555Tail, subdomain B (acidic)Add BLAST111

Domaini

The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.

Sequence similaritiesi

Belongs to the intermediate filament family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IGME. Eukaryota.
ENOG410XPTM. LUCA.
GeneTreeiENSGT00830000128228.
HOGENOMiHOG000230977.
HOVERGENiHBG013015.
InParanoidiP02548.
KOiK04572.
OMAiRSFPTYY.
OrthoDBiEOG091G12MK.
TreeFamiTF330122.

Family and domain databases

InterProiIPR001664. IF.
IPR006821. Intermed_filament_DNA-bd.
IPR018039. Intermediate_filament_CS.
IPR027692. NF-L.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 1 hit.
PTHR23239:SF22. PTHR23239:SF22. 1 hit.
PfamiPF00038. Filament. 1 hit.
PF04732. Filament_head. 1 hit.
[Graphical view]
SMARTiSM01391. Filament. 1 hit.
[Graphical view]
PROSITEiPS00226. IF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02548-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSFSYEPYY STSYKRRYVE TPRVHISSVR SGYSTARSAY SSYSAPVSSS
60 70 80 90 100
LSVRRSYSSS SGSLMPSLES LDLSQVAAIS NDLKSIRTQE KAQLQDLNDR
110 120 130 140 150
FASFIERVHE LEQQNKVLEA ELLVLRQKHS EPSRFRALYE QEIRDLRLAA
160 170 180 190 200
EDATNEKQAL QGEREGLEET LRNLQARYEE EVLSREDAEG RLMEARKGAD
210 220 230 240 250
EAALARAELE KRIDSLMDEI AFLKKVHEEE IAELQAQIQY AQISVEMDVS
260 270 280 290 300
SKPDLSAALK DIRAQYEKLA AKNMQNAEEW FKSRFTVLTE SAAKNTDAVR
310 320 330 340 350
AAKDEVSESR RLLKAKTLEI EACRGMNEAL EKQLQELEDK QNADISAMQD
360 370 380 390 400
TINKLENELR TTKSEMARYL KEYQDLLNVK MALDIEIAAY RKLLEGEETR
410 420 430 440 450
LSFTSVGSLT TGYTQSSQVF GRSAYGGLQT SSYLMSARSF PSYYTSHVQE
460 470 480 490 500
EQIEVEETIE AAKAEEAKDE PPSEGEAEEE EKEKEEAEAE AEAEAEAEAE
510 520 530 540 550
EEEGAQEEEA AKEDAEEAKE EEGGEGEEAE ETKEAEEEEK KDEGAGEEQA

TKKKD
Length:555
Mass (Da):62,646
Last modified:January 23, 2007 - v3
Checksum:iD772A27EE1F1DCD3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti495 – 501Missing AA sequence (PubMed:3884373).Curated7
Sequence conflicti510A → AEA AA sequence (PubMed:3884373).Curated1

Mass spectrometryi

Molecular mass is 62600 Da from positions 2 - 555. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U83919 mRNA. Translation: AAB41543.1.
BC118240 mRNA. Translation: AAI18241.1.
PIRiJW0094.
RefSeqiNP_776546.1. NM_174121.1.
UniGeneiBt.89013.

Genome annotation databases

EnsembliENSBTAT00000029264; ENSBTAP00000029264; ENSBTAG00000021949.
GeneIDi281348.
KEGGibta:281348.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U83919 mRNA. Translation: AAB41543.1.
BC118240 mRNA. Translation: AAI18241.1.
PIRiJW0094.
RefSeqiNP_776546.1. NM_174121.1.
UniGeneiBt.89013.

3D structure databases

ProteinModelPortaliP02548.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP02548. 1 interactor.
STRINGi9913.ENSBTAP00000029264.

PTM databases

iPTMnetiP02548.

Proteomic databases

PaxDbiP02548.
PeptideAtlasiP02548.
PRIDEiP02548.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000029264; ENSBTAP00000029264; ENSBTAG00000021949.
GeneIDi281348.
KEGGibta:281348.

Organism-specific databases

CTDi4747.

Phylogenomic databases

eggNOGiENOG410IGME. Eukaryota.
ENOG410XPTM. LUCA.
GeneTreeiENSGT00830000128228.
HOGENOMiHOG000230977.
HOVERGENiHBG013015.
InParanoidiP02548.
KOiK04572.
OMAiRSFPTYY.
OrthoDBiEOG091G12MK.
TreeFamiTF330122.

Enzyme and pathway databases

ReactomeiR-BTA-438066. Unblocking of NMDA receptor, glutamate binding and activation.
R-BTA-442729. CREB phosphorylation through the activation of CaMKII.
R-BTA-442982. Ras activation uopn Ca2+ infux through NMDA receptor.
R-BTA-5673001. RAF/MAP kinase cascade.

Gene expression databases

BgeeiENSBTAG00000021949.

Family and domain databases

InterProiIPR001664. IF.
IPR006821. Intermed_filament_DNA-bd.
IPR018039. Intermediate_filament_CS.
IPR027692. NF-L.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 1 hit.
PTHR23239:SF22. PTHR23239:SF22. 1 hit.
PfamiPF00038. Filament. 1 hit.
PF04732. Filament_head. 1 hit.
[Graphical view]
SMARTiSM01391. Filament. 1 hit.
[Graphical view]
PROSITEiPS00226. IF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNFL_BOVIN
AccessioniPrimary (citable) accession number: P02548
Secondary accession number(s): P79127, Q17QQ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 144 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

NF-L is the most abundant of the three neurofilament proteins and, like the other nonepithelial intermediate filament proteins, it can form homopolymeric 10-nm filaments.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.