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Protein

Neurofilament light polypeptide

Gene

NEFL

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.

Miscellaneous

NF-L is the most abundant of the three neurofilament proteins and, like the other nonepithelial intermediate filament proteins, it can form homopolymeric 10-nm filaments.

GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-BTA-438066 Unblocking of NMDA receptor, glutamate binding and activation
R-BTA-442729 CREB phosphorylation through the activation of CaMKII
R-BTA-442982 Ras activation upon Ca2+ influx through NMDA receptor
R-BTA-5673001 RAF/MAP kinase cascade

Names & Taxonomyi

Protein namesi
Recommended name:
Neurofilament light polypeptide
Short name:
NF-L
Alternative name(s):
68 kDa neurofilament protein
Micro glutamic acid-rich protein
Neurofilament triplet L protein
Gene namesi
Name:NEFL
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 8

Organism-specific databases

VGNCiVGNC:31986 NEFL

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Intermediate filament

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000637862 – 555Neurofilament light polypeptideAdd BLAST554

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserine1 Publication1
Modified residuei23Asymmetric dimethylarginine; alternateBy similarity1
Modified residuei23Omega-N-methylarginine; alternateBy similarity1
Modified residuei30Omega-N-methylarginineBy similarity1
Modified residuei43PhosphotyrosineBy similarity1
Modified residuei56Phosphoserine1 Publication1
Modified residuei67Phosphoserine1 Publication1
Modified residuei103PhosphoserineBy similarity1
Modified residuei473Phosphoserine1 Publication1
Modified residuei532PhosphothreonineBy similarity1

Post-translational modificationi

O-glycosylated.By similarity
Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.By similarity
Ubiquitinated in the presence of TRIM2 and UBE2D1.By similarity

Keywords - PTMi

Acetylation, Glycoprotein, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP02548
PeptideAtlasiP02548
PRIDEiP02548

PTM databases

iPTMnetiP02548

Expressioni

Gene expression databases

BgeeiENSBTAG00000021949

Interactioni

Subunit structurei

Interacts with ARHGEF28. Interacts with TRIM2.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiP02548, 1 interactor
STRINGi9913.ENSBTAP00000029264

Structurei

3D structure databases

ProteinModelPortaliP02548
SMRiP02548
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini90 – 401IF rodPROSITE-ProRule annotationAdd BLAST312

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 93HeadAdd BLAST92
Regioni94 – 125Coil 1AAdd BLAST32
Regioni126 – 138Linker 1Add BLAST13
Regioni139 – 234Coil 1BAdd BLAST96
Regioni235 – 253Linker 12Add BLAST19
Regioni254 – 272Coil 2AAdd BLAST19
Regioni273 – 281Linker 29
Regioni282 – 397Coil 2BAdd BLAST116
Regioni398 – 555TailAdd BLAST158
Regioni398 – 444Tail, subdomain AAdd BLAST47
Regioni445 – 555Tail, subdomain B (acidic)Add BLAST111

Domaini

The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.

Sequence similaritiesi

Belongs to the intermediate filament family.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IGME Eukaryota
ENOG410XPTM LUCA
GeneTreeiENSGT00910000143989
HOGENOMiHOG000230977
HOVERGENiHBG013015
InParanoidiP02548
KOiK04572
OMAiEMDVSSK
OrthoDBiEOG091G12MK
TreeFamiTF330122

Family and domain databases

InterProiView protein in InterPro
IPR001664 IF
IPR018039 IF_conserved
IPR039008 IF_rod_dom
IPR006821 Intermed_filament_DNA-bd
IPR027692 NF-L
PANTHERiPTHR23239 PTHR23239, 1 hit
PTHR23239:SF22 PTHR23239:SF22, 1 hit
PfamiView protein in Pfam
PF00038 Filament, 1 hit
PF04732 Filament_head, 1 hit
SMARTiView protein in SMART
SM01391 Filament, 1 hit
PROSITEiView protein in PROSITE
PS00226 IF_ROD_1, 1 hit
PS51842 IF_ROD_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02548-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSFSYEPYY STSYKRRYVE TPRVHISSVR SGYSTARSAY SSYSAPVSSS
60 70 80 90 100
LSVRRSYSSS SGSLMPSLES LDLSQVAAIS NDLKSIRTQE KAQLQDLNDR
110 120 130 140 150
FASFIERVHE LEQQNKVLEA ELLVLRQKHS EPSRFRALYE QEIRDLRLAA
160 170 180 190 200
EDATNEKQAL QGEREGLEET LRNLQARYEE EVLSREDAEG RLMEARKGAD
210 220 230 240 250
EAALARAELE KRIDSLMDEI AFLKKVHEEE IAELQAQIQY AQISVEMDVS
260 270 280 290 300
SKPDLSAALK DIRAQYEKLA AKNMQNAEEW FKSRFTVLTE SAAKNTDAVR
310 320 330 340 350
AAKDEVSESR RLLKAKTLEI EACRGMNEAL EKQLQELEDK QNADISAMQD
360 370 380 390 400
TINKLENELR TTKSEMARYL KEYQDLLNVK MALDIEIAAY RKLLEGEETR
410 420 430 440 450
LSFTSVGSLT TGYTQSSQVF GRSAYGGLQT SSYLMSARSF PSYYTSHVQE
460 470 480 490 500
EQIEVEETIE AAKAEEAKDE PPSEGEAEEE EKEKEEAEAE AEAEAEAEAE
510 520 530 540 550
EEEGAQEEEA AKEDAEEAKE EEGGEGEEAE ETKEAEEEEK KDEGAGEEQA

TKKKD
Length:555
Mass (Da):62,646
Last modified:January 23, 2007 - v3
Checksum:iD772A27EE1F1DCD3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti495 – 501Missing AA sequence (PubMed:3884373).Curated7
Sequence conflicti510A → AEA AA sequence (PubMed:3884373).Curated1

Mass spectrometryi

Molecular mass is 62600 Da from positions 2 - 555. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U83919 mRNA Translation: AAB41543.1
BC118240 mRNA Translation: AAI18241.1
PIRiJW0094
RefSeqiNP_776546.1, NM_174121.1
UniGeneiBt.89013

Genome annotation databases

EnsembliENSBTAT00000029264; ENSBTAP00000029264; ENSBTAG00000021949
GeneIDi281348
KEGGibta:281348

Similar proteinsi

Entry informationi

Entry nameiNFL_BOVIN
AccessioniPrimary (citable) accession number: P02548
Secondary accession number(s): P79127, Q17QQ0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 152 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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