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P02548

- NFL_BOVIN

UniProt

P02548 - NFL_BOVIN

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Protein

Neurofilament light polypeptide

Gene
NEFL
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.

GO - Molecular functioni

  1. identical protein binding Source: UniProtKB
  2. protein C-terminus binding Source: UniProtKB
  3. structural constituent of cytoskeleton Source: UniProtKB

GO - Biological processi

  1. anterograde axon cargo transport Source: UniProtKB
  2. axon transport of mitochondrion Source: UniProtKB
  3. intermediate filament bundle assembly Source: AgBase
  4. intermediate filament organization Source: AgBase
  5. locomotion Source: AgBase
  6. microtubule cytoskeleton organization Source: AgBase
  7. negative regulation of neuron apoptotic process Source: AgBase
  8. neurofilament bundle assembly Source: UniProtKB
  9. neurofilament cytoskeleton organization Source: AgBase
  10. neuromuscular process controlling balance Source: AgBase
  11. neuron projection morphogenesis Source: AgBase
  12. peripheral nervous system axon regeneration Source: AgBase
  13. positive regulation of axonogenesis Source: AgBase
  14. regulation of axon diameter Source: AgBase
  15. retrograde axon cargo transport Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Neurofilament light polypeptide
Short name:
NF-L
Alternative name(s):
68 kDa neurofilament protein
Micro glutamic acid-rich protein
Neurofilament triplet L protein
Gene namesi
Name:NEFL
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 8

Subcellular locationi

GO - Cellular componenti

  1. axon Source: AgBase
  2. intermediate filament Source: AgBase
  3. neurofilament Source: AgBase
Complete GO annotation...

Keywords - Cellular componenti

Intermediate filament

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 555554Neurofilament light polypeptidePRO_0000063786Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei43 – 431Phosphotyrosine By similarity
Modified residuei56 – 561Phosphoserine1 Publication
Modified residuei67 – 671Phosphoserine1 Publication
Modified residuei473 – 4731Phosphoserine1 Publication

Post-translational modificationi

O-glycosylated By similarity.
Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization By similarity.1 Publication
Ubiquitinated in the presence of TRIM2 and UBE2D1 By similarity.

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP02548.
PRIDEiP02548.

Interactioni

Subunit structurei

Interacts with ARHGEF28 By similarity. Interacts with TRIM2 By similarity.

Protein-protein interaction databases

IntActiP02548. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP02548.
SMRiP02548. Positions 88-124, 324-396.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 9392HeadAdd
BLAST
Regioni94 – 397304RodAdd
BLAST
Regioni94 – 12532Coil 1AAdd
BLAST
Regioni126 – 13813Linker 1Add
BLAST
Regioni139 – 23496Coil 1BAdd
BLAST
Regioni235 – 25319Linker 12Add
BLAST
Regioni254 – 27219Coil 2AAdd
BLAST
Regioni273 – 2819Linker 2
Regioni282 – 397116Coil 2BAdd
BLAST
Regioni398 – 555158TailAdd
BLAST
Regioni398 – 44447Tail, subdomain AAdd
BLAST
Regioni445 – 555111Tail, subdomain B (acidic)Add
BLAST

Domaini

The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG145720.
GeneTreeiENSGT00750000117235.
HOGENOMiHOG000230977.
HOVERGENiHBG013015.
InParanoidiP02548.
KOiK04572.
OMAiARNMQNA.
OrthoDBiEOG7FV3Q8.
TreeFamiTF330122.

Family and domain databases

InterProiIPR001664. IF.
IPR006821. Intermed_filament_DNA-bd.
IPR018039. Intermediate_filament_CS.
IPR027692. NF-L.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 1 hit.
PTHR23239:SF22. PTHR23239:SF22. 1 hit.
PfamiPF00038. Filament. 1 hit.
PF04732. Filament_head. 1 hit.
[Graphical view]
PROSITEiPS00226. IF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02548-1 [UniParc]FASTAAdd to Basket

« Hide

MSSFSYEPYY STSYKRRYVE TPRVHISSVR SGYSTARSAY SSYSAPVSSS    50
LSVRRSYSSS SGSLMPSLES LDLSQVAAIS NDLKSIRTQE KAQLQDLNDR 100
FASFIERVHE LEQQNKVLEA ELLVLRQKHS EPSRFRALYE QEIRDLRLAA 150
EDATNEKQAL QGEREGLEET LRNLQARYEE EVLSREDAEG RLMEARKGAD 200
EAALARAELE KRIDSLMDEI AFLKKVHEEE IAELQAQIQY AQISVEMDVS 250
SKPDLSAALK DIRAQYEKLA AKNMQNAEEW FKSRFTVLTE SAAKNTDAVR 300
AAKDEVSESR RLLKAKTLEI EACRGMNEAL EKQLQELEDK QNADISAMQD 350
TINKLENELR TTKSEMARYL KEYQDLLNVK MALDIEIAAY RKLLEGEETR 400
LSFTSVGSLT TGYTQSSQVF GRSAYGGLQT SSYLMSARSF PSYYTSHVQE 450
EQIEVEETIE AAKAEEAKDE PPSEGEAEEE EKEKEEAEAE AEAEAEAEAE 500
EEEGAQEEEA AKEDAEEAKE EEGGEGEEAE ETKEAEEEEK KDEGAGEEQA 550
TKKKD 555
Length:555
Mass (Da):62,646
Last modified:January 23, 2007 - v3
Checksum:iD772A27EE1F1DCD3
GO

Mass spectrometryi

Molecular mass is 62600 Da from positions 2 - 555. Determined by MALDI. 1 Publication

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti495 – 5017Missing AA sequence 1 Publication
Sequence conflicti510 – 5101A → AEA AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U83919 mRNA. Translation: AAB41543.1.
BC118240 mRNA. Translation: AAI18241.1.
PIRiJW0094.
RefSeqiNP_776546.1. NM_174121.1.
UniGeneiBt.89013.

Genome annotation databases

EnsembliENSBTAT00000029264; ENSBTAP00000029264; ENSBTAG00000021949.
GeneIDi281348.
KEGGibta:281348.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U83919 mRNA. Translation: AAB41543.1 .
BC118240 mRNA. Translation: AAI18241.1 .
PIRi JW0094.
RefSeqi NP_776546.1. NM_174121.1.
UniGenei Bt.89013.

3D structure databases

ProteinModelPortali P02548.
SMRi P02548. Positions 88-124, 324-396.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P02548. 1 interaction.

Proteomic databases

PaxDbi P02548.
PRIDEi P02548.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000029264 ; ENSBTAP00000029264 ; ENSBTAG00000021949 .
GeneIDi 281348.
KEGGi bta:281348.

Organism-specific databases

CTDi 4747.

Phylogenomic databases

eggNOGi NOG145720.
GeneTreei ENSGT00750000117235.
HOGENOMi HOG000230977.
HOVERGENi HBG013015.
InParanoidi P02548.
KOi K04572.
OMAi ARNMQNA.
OrthoDBi EOG7FV3Q8.
TreeFami TF330122.

Miscellaneous databases

NextBioi 20805356.

Family and domain databases

InterProi IPR001664. IF.
IPR006821. Intermed_filament_DNA-bd.
IPR018039. Intermediate_filament_CS.
IPR027692. NF-L.
[Graphical view ]
PANTHERi PTHR23239. PTHR23239. 1 hit.
PTHR23239:SF22. PTHR23239:SF22. 1 hit.
Pfami PF00038. Filament. 1 hit.
PF04732. Filament_head. 1 hit.
[Graphical view ]
PROSITEi PS00226. IF. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Hill W.D., Zhang L., Balin B.J., Sprinkle T.J.
    Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Holstein.
    Tissue: Brain.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Basal ganglia.
  3. "Brain micro glutamic acid-rich protein is the C-terminal endpiece of the neurofilament 68-kDa protein as determined by the primary sequence."
    Isobe T., Okuyama T.
    FEBS Lett. 182:389-392(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 469-555.
  4. "Identification of endogenous phosphorylation sites of bovine medium and low molecular weight neurofilament proteins by tandem mass spectrometry."
    Trimpin S., Mixon A.E., Stapels M.D., Kim M.Y., Spencer P.S., Deinzer M.L.
    Biochemistry 43:2091-2105(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION AT SER-2, PHOSPHORYLATION AT SER-56; SER-67 AND SER-473, MASS SPECTROMETRY.

Entry informationi

Entry nameiNFL_BOVIN
AccessioniPrimary (citable) accession number: P02548
Secondary accession number(s): P79127, Q17QQ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 123 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

NF-L is the most abundant of the three neurofilament proteins and, like the other nonepithelial intermediate filament proteins, it can form homopolymeric 10-nm filaments.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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