P02548 (NFL_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Neurofilament light polypeptide
Short name=NF-L

Alternative name(s):
68 kDa neurofilament protein
Micro glutamic acid-rich protein
Neurofilament triplet L protein

Gene names

Name:

NEFL

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	555 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
Subunit structure	Interacts with ARHGEF28 By similarity. Interacts with TRIM2 By similarity.
Domain	The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.
Post-translational modification	O-glycosylated By similarity. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization By similarity. Ref.4 Ubiquitinated in the presence of TRIM2 and UBE2D1 By similarity.
Miscellaneous	NF-L is the most abundant of the three neurofilament proteins and, like the other nonepithelial intermediate filament proteins, it can form homopolymeric 10-nm filaments.
Sequence similarities	Belongs to the intermediate filament family.
Mass spectrometry	Molecular mass is 62600 Da from positions 2 - 555. Determined by MALDI. Ref.4

Ontologies

Keywords
Cellular component	Intermediate filament
Domain	Coiled coil
PTM	Acetylation Glycoprotein Phosphoprotein Ubl conjugation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	anterograde axon cargo transport Inferred from sequence or structural similarity. Source: UniProtKB axon transport of mitochondrion Inferred from sequence or structural similarity. Source: UniProtKB locomotion Inferred from sequence or structural similarity. Source: AgBase microtubule cytoskeleton organization Inferred from sequence or structural similarity. Source: AgBase negative regulation of neuron apoptotic process Inferred from sequence or structural similarity. Source: AgBase neurofilament bundle assembly Inferred from sequence or structural similarity. Source: UniProtKB neuromuscular process controlling balance Inferred from sequence or structural similarity. Source: AgBase peripheral nervous system axon regeneration Inferred from sequence or structural similarity. Source: AgBase positive regulation of axonogenesis Inferred from sequence or structural similarity. Source: AgBase regulation of axon diameter Inferred from sequence or structural similarity. Source: AgBase retrograde axon cargo transport Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	neurofilament Inferred from sequence or structural similarity. Source: AgBase
Molecular_function	identical protein binding Inferred from sequence or structural similarity. Source: UniProtKB protein C-terminus binding Inferred from sequence or structural similarity. Source: UniProtKB structural constituent of cytoskeleton Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 555	554	Neurofilament light polypeptide		PRO_0000063786
Regions
	Region	2 – 93	92	Head
	Region	94 – 397	304	Rod
	Region	94 – 125	32	Coil 1A
	Region	126 – 138	13	Linker 1
	Region	139 – 234	96	Coil 1B
	Region	235 – 253	19	Linker 12
	Region	254 – 272	19	Coil 2A
	Region	273 – 281	9	Linker 2
	Region	282 – 397	116	Coil 2B
	Region	398 – 555	158	Tail
	Region	398 – 444	47	Tail, subdomain A
	Region	445 – 555	111	Tail, subdomain B (acidic)
Amino acid modifications
	Modified residue	2	1	N-acetylserine Ref.4
	Modified residue	43	1	Phosphotyrosine By similarity
	Modified residue	56	1	Phosphoserine Ref.4
	Modified residue	67	1	Phosphoserine Ref.4
	Modified residue	425	1	Phosphotyrosine By similarity
	Modified residue	433	1	Phosphotyrosine By similarity
	Modified residue	473	1	Phosphoserine Ref.4
Experimental info
	Sequence conflict	495 – 501	7	Missing AA sequence Ref.3
	Sequence conflict	510	1	A → AEA AA sequence Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P02548 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: D772A27EE1F1DCD3	FASTA	555	62,646
	10 20 30 40 50 60 MSSFSYEPYY STSYKRRYVE TPRVHISSVR SGYSTARSAY SSYSAPVSSS LSVRRSYSSS 70 80 90 100 110 120 SGSLMPSLES LDLSQVAAIS NDLKSIRTQE KAQLQDLNDR FASFIERVHE LEQQNKVLEA 130 140 150 160 170 180 ELLVLRQKHS EPSRFRALYE QEIRDLRLAA EDATNEKQAL QGEREGLEET LRNLQARYEE 190 200 210 220 230 240 EVLSREDAEG RLMEARKGAD EAALARAELE KRIDSLMDEI AFLKKVHEEE IAELQAQIQY 250 260 270 280 290 300 AQISVEMDVS SKPDLSAALK DIRAQYEKLA AKNMQNAEEW FKSRFTVLTE SAAKNTDAVR 310 320 330 340 350 360 AAKDEVSESR RLLKAKTLEI EACRGMNEAL EKQLQELEDK QNADISAMQD TINKLENELR 370 380 390 400 410 420 TTKSEMARYL KEYQDLLNVK MALDIEIAAY RKLLEGEETR LSFTSVGSLT TGYTQSSQVF 430 440 450 460 470 480 GRSAYGGLQT SSYLMSARSF PSYYTSHVQE EQIEVEETIE AAKAEEAKDE PPSEGEAEEE 490 500 510 520 530 540 EKEKEEAEAE AEAEAEAEAE EEEGAQEEEA AKEDAEEAKE EEGGEGEEAE ETKEAEEEEK 550 KDEGAGEEQA TKKKD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Hill W.D., Zhang L., Balin B.J., Sprinkle T.J. Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Holstein. Tissue: Brain.
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Basal ganglia.
[3]	"Brain micro glutamic acid-rich protein is the C-terminal endpiece of the neurofilament 68-kDa protein as determined by the primary sequence." Isobe T., Okuyama T. FEBS Lett. 182:389-392(1985) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 469-555.
[4]	"Identification of endogenous phosphorylation sites of bovine medium and low molecular weight neurofilament proteins by tandem mass spectrometry." Trimpin S., Mixon A.E., Stapels M.D., Kim M.Y., Spencer P.S., Deinzer M.L. Biochemistry 43:2091-2105(2004) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION AT SER-2, PHOSPHORYLATION AT SER-56; SER-67 AND SER-473, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U83919 mRNA. Translation: AAB41543.1. BC118240 mRNA. Translation: AAI18241.1.
IPI	IPI00717196.
PIR	JW0094.
RefSeq	NP_776546.1. NM_174121.1.
UniGene	Bt.89013.
3D structure databases
ProteinModelPortal	P02548.
SMR	P02548. Positions 88-124, 324-396.
ModBase	Search...
Protein-protein interaction databases
IntAct	P02548. 1 interaction.
Proteomic databases
PaxDb	P02548.
PRIDE	P02548.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000029264; ENSBTAP00000029264; ENSBTAG00000021949.
GeneID	281348.
KEGG	bta:281348.
Organism-specific databases
CTD	4747.
Phylogenomic databases
eggNOG	NOG145720.
GeneTree	ENSGT00690000102043.
HOGENOM	HOG000230977.
HOVERGEN	HBG013015.
InParanoid	P02548.
KO	K04572.
OMA	PRVHISS.
OrthoDB	EOG42Z4QC.
Family and domain databases
InterPro	IPR016044. F. IPR001664. IF. IPR006821. Intermed_filament_DNA-bd. IPR018039. Intermediate_filament_CS. [Graphical view]
PANTHER	PTHR23239. PTHR23239. 1 hit.
Pfam	PF00038. Filament. 1 hit. PF04732. Filament_head. 1 hit. [Graphical view]
PROSITE	PS00226. IF. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20805356.

Entry information

Entry name

NFL_BOVIN

Accession

Primary (citable) accession number: P02548
Secondary accession number(s): P79127, Q17QQ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 113 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program