Reviewed, UniProtKB/Swiss-Prot P02526 (CRGB_BOVIN)

Names and origin

Protein names

Recommended name:
    Gamma-crystallin B
Alternative name(s):
    Gamma-B-crystallin
    Gamma-crystallin II

Gene names

Name:

CRYGB

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	175 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Crystallins are the dominant structural components of the vertebrate eye lens.
Domain	Has a two-domain beta-structure, folded into four very similar Greek key motifs.
Sequence similarities	Belongs to the beta/gamma-crystallin family. Contains 4 beta/gamma crystallin 'Greek key' domains.

Ontologies

Keywords
Cellular component	Eye lens protein
Domain	Repeat
PTM	Glycation Glycoprotein Oxidation Phosphoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Molecular function	structural constituent of eye lens Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed

Chain

2 – 175

174

Gamma-crystallin B

PRO_0000057581

Regions

Domain

2 – 40

39

Beta/gamma crystallin 'Greek key' 1

Domain

41 – 83

43

Beta/gamma crystallin 'Greek key' 2

Domain

89 – 129

41

Beta/gamma crystallin 'Greek key' 3

Domain

130 – 172

43

Beta/gamma crystallin 'Greek key' 4

Region

84 – 88

5

Connecting peptide

Sites

Site

69

1

Susceptible to oxidation By similarity

Site

70

1

Susceptible to oxidation By similarity

Amino acid modifications

Modified residue

63

1

Phosphotyrosine By similarity

Modified residue

66

1

Phosphotyrosine By similarity

Glycosylation

3

1

N-linked (Glc) (glycation); in vitro

Disulfide bond

19 ↔ 23

Experimental info

Sequence conflict

120

1

T → S in CAA25518. Ref.2

Secondary structure

1

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175

Helix Strand Turn

Details...

Beta strand

3 – 9

7

Helix

10 – 12

3

Beta strand

13 – 19

7

Turn

27 – 29

3

Beta strand

34 – 41

8

Beta strand

43 – 48

6

Turn

49 – 51

3

Beta strand

52 – 58

7

Beta strand

60 – 65

6

Helix

66 – 69

4

Beta strand

72 – 74

3

Beta strand

78 – 81

4

Beta strand

90 – 96

7

Turn

97 – 99

3

Beta strand

100 – 106

7

Helix

113 – 117

5

Beta strand

124 – 130

7

Beta strand

132 – 137

6

Turn

138 – 140

3

Beta strand

141 – 147

7

Beta strand

149 – 152

4

Helix

155 – 158

4

Beta strand

167 – 170

4

Sequences

	Sequence		Length	Mass (Da)
	P02526-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 0CF283DA837CC593	FASTA	175	21,097
	10 20 30 40 50 60 MGKITFYEDR GFQGHCYECS SDCPNLQPYF SRCNSIRVDS GCWMLYERPN YQGHQYFLRR 70 80 90 100 110 120 GDYPDYQQWM GFNDSIRSCR LIPQHTGTFR MRIYERDDFR GQMSEITDDC PSLQDRFHLT 130 140 150 160 170 EVHSLNVLEG SWVLYEMPSY RGRQYLLRPG EYRRYLDWGA MNAKVGSLRR VMDFY

References

[1]	"cDNA clones encoding bovine gamma-crystallins." Hay R.E., Woods W.D., Church R.L., Petrash J.M. Biochem. Biophys. Res. Commun. 146:332-338(1987) [PubMed: 3606621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Complete nucleotide sequence of a cDNA derived from calf lens gamma-crystallin mRNA: presence of Alu I-like DNA sequences." Bhat S.P., Spector A. DNA 3:287-295(1984) [PubMed: 6092016] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lens.
[3]	Wistow G. Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lens.
[4]	"Physicochemical characterization of gamma-crystallins from bovine lens -- hydrodynamic and biochemical properties." Chiou S.H., Azari P., Himmel M.E. J. Protein Chem. 7:67-80(1988) [PubMed: 3255364] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-26.
[5]	"The disulfide content of calf gamma-crystallin." McDermott M.J., Gawinowicz-Kolks M.A., Chiesa R., Spector A. Arch. Biochem. Biophys. 262:609-619(1988) [PubMed: 3364984] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-26, DISULFIDE BOND.
[6]	"Identification of the glycation site of lens gamma B-crystallin by fast atom bombardment tandem mass spectrometry." Smith J.B., Hanson S.R., Cerny R.L., Zhao H.R., Abraham E.C. Anal. Biochem. 243:186-189(1996) [PubMed: 8954545] [Abstract] Cited for: GLYCATION AT LYS-3, MASS SPECTROMETRY.
[7]	"The molecular structure and stability of the eye lens: X-ray analysis of gamma-crystallin II." Blundell T.L., Lindley P., Miller L., Moss D., Slingsby C., Tickle I., Turnell B., Wistow G. Nature 289:771-777(1981) [PubMed: 7464942] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS), SEQUENCE REVISION.
[8]	"X-ray analysis of the eye lens protein gamma-II crystallin at 1.9-A resolution." Wistow G., Turnell B., Summers L., Slingsby C., Moss D., Miller L., Lindley P., Blundell T.L. J. Mol. Biol. 170:175-202(1983) [PubMed: 6631960] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
[9]	"Structure of the bovine eye lens protein gammaB (gammaII)-crystallin at 1.47 A." Najmudin S., Nalini V., Dreissen H.P.C., Slingsby C., Blundell T.L., Moss D.S., Lindley P.F. Acta Crystallogr. D 49:223-233(1993) [PubMed: 15299528] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.47 ANGSTROMS).
[10]	"An eye lens protein-water structure: 1.2-A resolution structure of gammaB-crystallin at 150 K." Kumaraswamy V.S., Lindley P.F., Slingsby C., Glover I.D. Acta Crystallogr. D 52:611-622(1996) [PubMed: 15299624] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.2 ANGSTROMS).
[11]	"X-ray structures of three interface mutants of gammaB-crystallin from bovine eye lens." Palme S., Jaenicke R., Slingsby C. Protein Sci. 7:611-618(1998) [PubMed: 9541393] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS).
[12]	"Unusual domain pairing in a mutant of bovine lens gammaB-crystallin." Palme S., Jaenicke R., Slingsby C. J. Mol. Biol. 279:1053-1059(1998) [PubMed: 9642083] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF MUTANT ALA-57.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X01036 mRNA. Translation: CAA25518.1.
M16894 mRNA. Translation: AAA30476.1.
EF208024 mRNA. Translation: ABM97504.1.

PIR

CYBOG. A29655.

RefSeq

NP_001013612.1.

UniGene

Bt.399

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1AMM	X-ray	1.20	A	1-175	[»]
1DSL	X-ray	1.55	A	88-175	[»]
1GAM	X-ray	2.60	A/B	88-173	[»]
1GCS	X-ray	2.00	A	1-175	[»]
1I5I	X-ray	2.40	A	1-175	[»]
4GCR	X-ray	1.47	A	1-175	[»]

ModBase

Search...

Genome annotation databases

Ensembl

ENSBTAG00000021770. Bos taurus. [Contig view]

GeneID

281720.

KEGG

bta:281720.

Phylogenomic databases

HOVERGEN

P02526.

Family and domain databases

InterPro

IPR001064. Crystallin.
[Graphical view]

Gene3D

G3DSA:2.60.20.10. Crystallin. 2 hits.

Pfam

PF00030. Crystall. 2 hits.
[Graphical view]

PRINTS

PR01367. BGCRYSTALLIN.

SMART

SM00247. XTALbg. 2 hits.
[Graphical view]

PROSITE

PS50915. CRYSTALLIN_BETA_GAMMA. 4 hits.
[Graphical view]

ProtoNet

Search...

Other Resources

LinkHub

P02526.

Entry information

Entry name

CRGB_BOVIN

Accession

Primary (citable) accession number: P02526
Secondary accession number(s): A2TJU8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	November 4, 2008
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)