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Protein

Beta-crystallin B3

Gene

Crybb3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Crystallins are the dominant structural components of the vertebrate eye lens.

GO - Molecular functioni

  • structural constituent of eye lens Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Eye lens protein

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-crystallin B3
Alternative name(s):
Beta-B3 crystallin
Cleaved into the following chain:
Gene namesi
Name:Crybb3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi61980. Crybb3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 211211Beta-crystallin B3PRO_0000423201Add
BLAST
Initiator methionineiRemoved; alternate1 Publication
Chaini2 – 211210Beta-crystallin B3, N-terminally processedPRO_0000057562Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei2 – 21N-acetylalanine; in Beta-crystallin B3, N-terminally processed1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP02524.

PTM databases

PhosphoSiteiP02524.

Expressioni

Gene expression databases

GenevisibleiP02524. RN.

Interactioni

Subunit structurei

Homo/heterodimer, or complexes of higher-order. The structure of beta-crystallin oligomers seems to be stabilized through interactions between the N-terminal arms (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000067156.

Structurei

3D structure databases

ProteinModelPortaliP02524.
SMRiP02524. Positions 22-202.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 6340Beta/gamma crystallin 'Greek key' 1PROSITE-ProRule annotationAdd
BLAST
Domaini64 – 10845Beta/gamma crystallin 'Greek key' 2PROSITE-ProRule annotationAdd
BLAST
Domaini114 – 15542Beta/gamma crystallin 'Greek key' 3PROSITE-ProRule annotationAdd
BLAST
Domaini156 – 19843Beta/gamma crystallin 'Greek key' 4PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 2322N-terminal armAdd
BLAST
Regioni109 – 1135Connecting peptide
Regioni200 – 21112C-terminal armAdd
BLAST

Domaini

Has a two-domain beta-structure, folded into four very similar Greek key motifs.

Sequence similaritiesi

Belongs to the beta/gamma-crystallin family.Curated
Contains 4 beta/gamma crystallin 'Greek key' domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IJYD. Eukaryota.
ENOG410Y7P1. LUCA.
GeneTreeiENSGT00760000118812.
HOVERGENiHBG003364.
InParanoidiP02524.
OMAiFGGRKME.
OrthoDBiEOG091G0JIZ.
PhylomeDBiP02524.

Family and domain databases

InterProiIPR001064. Beta/gamma_crystallin.
IPR033115. CRYBB3.
IPR011024. G_crystallin-rel.
[Graphical view]
PANTHERiPTHR11818:SF13. PTHR11818:SF13. 1 hit.
PfamiPF00030. Crystall. 2 hits.
[Graphical view]
PRINTSiPR01367. BGCRYSTALLIN.
SMARTiSM00247. XTALbg. 2 hits.
[Graphical view]
SUPFAMiSSF49695. SSF49695. 1 hit.
PROSITEiPS50915. CRYSTALLIN_BETA_GAMMA. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02524-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEQHGAPEQ AAASKSHGGL GGSYKVTVYE LENFQGKRCE LSAECPNLTE
60 70 80 90 100
SLLQKVGSIQ VESGPWLAFE RRAFRGEQFV LEKGDYPRWD AWSSSRRSDI
110 120 130 140 150
LLSLRPLHID GPDHKLHLFE NPAFSGRKME IVDDDVPSLW AHGFQDRVAS
160 170 180 190 200
IRVINGTWVG YEFPGYRGRQ YVFERGEFRH WNEWDANQPQ LQSVRRIRDQ
210
KWHKRGCFLS S
Length:211
Mass (Da):24,334
Last modified:January 23, 2007 - v5
Checksum:iD3B694BF858A94DB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti14 – 141S → N in CAA29328 (PubMed:3879970).Curated
Sequence conflicti88 – 881R → A in CAA29328 (PubMed:3879970).Curated

Mass spectrometryi

Molecular mass is 24271.5 Da from positions 2 - 211. Determined by ESI. Variant Leu-42.1 Publication
Molecular mass is 24245.7 Da from positions 2 - 211. Determined by ESI. Variant Ser-42.1 Publication

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti42 – 421S → L.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05899 mRNA. Translation: CAA29328.1.
AF287304 mRNA. Translation: AAF98363.1.
PIRiA02926. CYRTB3.
RefSeqiNP_113878.1. NM_031690.1.
XP_006249634.1. XM_006249572.1.
UniGeneiRn.19693.

Genome annotation databases

EnsembliENSRNOT00000071328; ENSRNOP00000067156; ENSRNOG00000047673.
GeneIDi64349.
KEGGirno:64349.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05899 mRNA. Translation: CAA29328.1.
AF287304 mRNA. Translation: AAF98363.1.
PIRiA02926. CYRTB3.
RefSeqiNP_113878.1. NM_031690.1.
XP_006249634.1. XM_006249572.1.
UniGeneiRn.19693.

3D structure databases

ProteinModelPortaliP02524.
SMRiP02524. Positions 22-202.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000067156.

PTM databases

PhosphoSiteiP02524.

Proteomic databases

PaxDbiP02524.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000071328; ENSRNOP00000067156; ENSRNOG00000047673.
GeneIDi64349.
KEGGirno:64349.

Organism-specific databases

CTDi1417.
RGDi61980. Crybb3.

Phylogenomic databases

eggNOGiENOG410IJYD. Eukaryota.
ENOG410Y7P1. LUCA.
GeneTreeiENSGT00760000118812.
HOVERGENiHBG003364.
InParanoidiP02524.
OMAiFGGRKME.
OrthoDBiEOG091G0JIZ.
PhylomeDBiP02524.

Miscellaneous databases

PROiP02524.

Gene expression databases

GenevisibleiP02524. RN.

Family and domain databases

InterProiIPR001064. Beta/gamma_crystallin.
IPR033115. CRYBB3.
IPR011024. G_crystallin-rel.
[Graphical view]
PANTHERiPTHR11818:SF13. PTHR11818:SF13. 1 hit.
PfamiPF00030. Crystall. 2 hits.
[Graphical view]
PRINTSiPR01367. BGCRYSTALLIN.
SMARTiSM00247. XTALbg. 2 hits.
[Graphical view]
SUPFAMiSSF49695. SSF49695. 1 hit.
PROSITEiPS50915. CRYSTALLIN_BETA_GAMMA. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCRBB3_RAT
AccessioniPrimary (citable) accession number: P02524
Secondary accession number(s): P70522, Q9ET12
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 115 of the entry and version 5 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.