50S ribosomal protein L15 - Escherichia coli (strain K12)

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P02413 (RL15_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 124. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
50S ribosomal protein L15

Gene names

Name:	rplO
Ordered Locus Names:	b3301, JW3263

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	144 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This protein binds the 5S rRNA. It is required for the late stages of subunit assembly, and is essential for 5S rRNA assembly onto the ribosome. HAMAP MF_01341_B
Subunit structure	Part of the 50S ribosomal subunit. Contacts the 5S rRNA. Ref.5
Sequence similarities	Belongs to the ribosomal protein L15P family.
Mass spectrometry	Molecular mass is 14980.1 Da from positions 1 - 144. Determined by MALDI. Ref.6

Ontologies

Keywords
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: InterPro
Cellular component	large ribosomal subunit Inferred from electronic annotation. Source: InterPro
Molecular function	rRNA binding Inferred from electronic annotation. Source: UniProtKB-KW structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 144

144

50S ribosomal protein L15 HAMAP MF_01341_B

PRO_0000104721

Secondary structure

144

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P02413 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 53D14CD948815FD9
Show »

FASTA

144

14,980

        10         20         30         40         50         60 
MRLNTLSPAE GSKKAGKRLG RGIGSGLGKT GGRGHKGQKS RSGGGVRRGF EGGQMPLYRR 

        70         80         90        100        110        120 
LPKFGFTSRK AAITAEIRLS DLAKVEGGVV DLNTLKAANI IGIQIEFAKV ILAGEVTTPV 

       130        140 
TVRGLRVTKG ARAAIEAAGG KIEE

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The primary structure of protein L15 located at the peptidyltransferase center of Escherichia coli ribosomes." Giorginis S., Chen R. FEBS Lett. 84:347-350(1977) [PubMed: 340263] [Abstract] Cited for: PROTEIN SEQUENCE. Strain: K.
[2]	"The spc ribosomal protein operon of Escherichia coli: sequence and cotranscription of the ribosomal protein genes and a protein export gene." Cerretti D.P., Dean D., Davis G.R., Bedwell D.M., Nomura M. Nucleic Acids Res. 11:2599-2616(1983) [PubMed: 6222285] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Incorporation of six additional proteins to complete the assembly map of the 50 S subunit from Escherichia coli ribosomes." Herold M., Nierhaus K.H. J. Biol. Chem. 262:8826-8833(1987) [PubMed: 3298242] [Abstract] Cited for: ASSEMBLY MAP OF THE 50S SUBUNIT. Strain: K12.
[6]	"Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry." Arnold R.J., Reilly J.P. Anal. Biochem. 269:105-112(1999) [PubMed: 10094780] [Abstract] Cited for: MASS SPECTROMETRY. Strain: K12 / ATCC 25404 / DSM 5698 / NCIMB 11290.
[7]	"Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement." Gao H., Sengupta J., Valle M., Korostelev A., Eswar N., Stagg S.M., Van Roey P., Agrawal R.K., Harvey S.C., Sali A., Chapman M.S., Frank J. Cell 113:789-801(2003) [PubMed: 12809609] [Abstract] Cited for: STRUCTURE BY ELECTRON MICROSCOPY (11.50 ANGSTROMS). Strain: MRE-600.
[8]	"Structures of the bacterial ribosome at 3.5 A resolution." Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W., Vila-Sanjurjo A., Holton J.M., Cate J.H.D. Science 310:827-834(2005) [PubMed: 16272117] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.46 ANGSTROMS) OF 2 DIFFERENT RIBOSOME STRUCTURES. Strain: MRE-600.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X01563 Genomic DNA. Translation: CAA25724.1.
U18997 Genomic DNA. Translation: AAA58098.1.
U00096 Genomic DNA. Translation: AAC76326.1.
AP009048 Genomic DNA. Translation: BAE77990.1.

PIR

R5EC15. A02794.

RefSeq

NP_417760.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1P85	electron microscopy	12.30	J	1-144	[»]
1P86	electron microscopy	11.50	J	1-144	[»]
1VS6	X-ray	3.46	L	1-144	[»]
1VS8	X-ray	3.46	L	1-144	[»]
1VT2	X-ray	3.30	L	1-144	[»]
2AW4	X-ray	3.46	L	1-144	[»]
2AWB	X-ray	3.46	L	1-144	[»]
2GYA	electron microscopy	15.00	J	4-143	[»]
2GYC	electron microscopy	15.00	J	4-143	[»]
2I2T	X-ray	3.22	L	1-144	[»]
2I2V	X-ray	3.22	L	1-144	[»]
2J28	electron microscopy	8.00	L	1-144	[»]
2QAM	X-ray	3.21	L	1-144	[»]
2QAO	X-ray	3.21	L	1-144	[»]
2QBA	X-ray	3.54	L	1-144	[»]
2QBC	X-ray	3.54	L	1-144	[»]
2QBE	X-ray	3.30	L	1-144	[»]
2QBG	X-ray	3.30	L	1-144	[»]
2QBI	X-ray	4.00	L	1-144	[»]
2QBK	X-ray	4.00	L	1-144	[»]
2QOV	X-ray	3.93	L	1-144	[»]
2QOX	X-ray	3.93	L	1-144	[»]
2QOZ	X-ray	3.50	L	1-144	[»]
2QP1	X-ray	3.50	L	1-144	[»]
2RDO	electron microscopy	9.10	L	1-144	[»]
2VHM	X-ray	3.74	L	1-144	[»]
2VHN	X-ray	3.74	L	1-144	[»]
2WWQ	electron microscopy	5.80	L	2-144	[»]
2Z4L	X-ray	4.45	L	1-144	[»]
2Z4N	X-ray	4.45	L	1-144	[»]
3BBX	electron microscopy	10.00	L	1-144	[»]
3DF2	X-ray	3.50	L	1-144	[»]
3DF4	X-ray	3.50	L	1-144	[»]
3E1B	electron microscopy	-	E	1-144	[»]
3E1D	electron microscopy	-	E	1-144	[»]
3FIK	electron microscopy	6.70	L	2-144	[»]
3I1N	X-ray	3.19	L	1-144	[»]
3I1P	X-ray	3.19	L	1-144	[»]
3I1R	X-ray	3.81	L	1-144	[»]
3I1T	X-ray	3.81	L	1-144	[»]
3I20	X-ray	3.71	L	1-144	[»]
3I22	X-ray	3.71	L	1-144	[»]
3IZT	electron microscopy	-	M	1-144	[»]
3IZU	electron microscopy	-	M	1-144	[»]
3J01	electron microscopy	-	L	1-144	[»]
3KCR	electron microscopy	-	L	1-144	[»]
3OAS	X-ray	3.25	L	2-144	[»]
3OAT	X-ray	3.25	L	2-144	[»]
3OFC	X-ray	3.19	L	2-144	[»]
3OFD	X-ray	3.19	L	2-144	[»]
3OFQ	X-ray	3.10	L	2-144	[»]
3OFR	X-ray	3.10	L	2-144	[»]
3OFZ	X-ray	3.29	L	2-144	[»]
3OG0	X-ray	3.29	L	2-144	[»]
3ORB	X-ray	3.30	L	1-144	[»]
3R8S	X-ray	3.00	L	2-144	[»]
3R8T	X-ray	3.00	L	2-144	[»]

ProteinModelPortal

P02413.

SMR

P02413. Positions 2-144.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-10752N.

IntAct

P02413. 77 interactions.

MINT

MINT-1260355.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000000709; EBESCP00000000709; EBESCG00000000591.
EBESCT00000016489; EBESCP00000015780; EBESCG00000015549.

GeneID

947798.

GenomeReviews

Gene locus JW3263 in contig AP009048_GR.
Gene locus b3301 in contig U00096_GR.

KEGG

ecj:JW3263.
eco:b3301.

PATRIC

32122034. VBIEscCol129921_3394.

Organism-specific databases

EchoBASE

EB0869.

EcoGene

EG10876. rplO.

Phylogenomic databases

eggNOG

COG0200.

GeneTree

EBGT00050000011243.

HOGENOM

HBG727142.

OMA

RKGRGHA.

PhylomeDB

P02413.

ProtClustDB

PRK05592.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10876-MONOMER.

Gene expression databases

Genevestigator

P02413.

Family and domain databases

HAMAP

MF_01341_B. Ribosomal_L15_B.
[Tree]

InterPro

IPR005749. Ribosomal_L15_bac-type.
IPR001196. Ribosomal_L15_CS.
IPR021131. Ribosomal_L18e/L15P.
[Graphical view]

K02876.

PANTHER

PTHR12934. Ribosom_L15_bac. 1 hit.

Pfam

PF00828. Ribosomal_L18e. 1 hit.
[Graphical view]

SUPFAM

SSF52080. Ribosomal_L18e/L15. 1 hit.

TIGRFAMs

TIGR01071. RplO_bact. 1 hit.

PROSITE

PS00475. RIBOSOMAL_L15. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RL15_ECOLI

Accession

Primary (citable) accession number: P02413
Secondary accession number(s): Q2M6W6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	January 25, 2012
This is version 124 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Ribosomal proteins

Ribosomal proteins families and list of entries

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents