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Reviewed, UniProtKB/Swiss-Prot P02413 (RL15_ECOLI)

Last modified June 16, 2009. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    50S ribosomal protein L15
Gene names
Name: rplO
Ordered Locus Names: b3301, JW3263
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length144 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

This protein binds the 5S rRNA. It is required for the late stages of subunit assembly, and is essential for 5S rRNA assembly onto the ribosome. HAMAP MF_01341

Subunit structure

Part of the 50S ribosomal subunit. Contacts the 5S rRNA. Ref.5

Sequence similarities

Belongs to the ribosomal protein L15P family.

Mass spectrometry

Molecular mass is 14980.1 Da from positions 1 - 144. Determined by MALDI. Ref.6

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 14414450S ribosomal protein L15 HAMAP MF_01341
PRO_0000104721

Secondary structure

.............. 144
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P02413-1 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 53D14CD948815FD9

FASTA14414,980
        10         20         30         40         50         60 
MRLNTLSPAE GSKKAGKRLG RGIGSGLGKT GGRGHKGQKS RSGGGVRRGF EGGQMPLYRR 

        70         80         90        100        110        120 
LPKFGFTSRK AAITAEIRLS DLAKVEGGVV DLNTLKAANI IGIQIEFAKV ILAGEVTTPV 

       130        140 
TVRGLRVTKG ARAAIEAAGG KIEE 

« Hide

References

« Hide 'large scale' references
[1]"The primary structure of protein L15 located at the peptidyltransferase center of Escherichia coli ribosomes."
Giorginis S., Chen R.
FEBS Lett. 84:347-350(1977) [PubMed: 340263] [Abstract]
Cited for: PROTEIN SEQUENCE.
Strain: K.
[2]"The spc ribosomal protein operon of Escherichia coli: sequence and cotranscription of the ribosomal protein genes and a protein export gene."
Cerretti D.P., Dean D., Davis G.R., Bedwell D.M., Nomura M.
Nucleic Acids Res. 11:2599-2616(1983) [PubMed: 6222285] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Incorporation of six additional proteins to complete the assembly map of the 50 S subunit from Escherichia coli ribosomes."
Herold M., Nierhaus K.H.
J. Biol. Chem. 262:8826-8833(1987) [PubMed: 3298242] [Abstract]
Cited for: ASSEMBLY MAP OF THE 50S SUBUNIT.
Strain: K12.
[6]"Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry."
Arnold R.J., Reilly J.P.
Anal. Biochem. 269:105-112(1999) [PubMed: 10094780] [Abstract]
Cited for: MASS SPECTROMETRY.
Strain: K12 / ATCC 25404 / DSM 5698 / NCIMB 11290.
[7]"Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement."
Gao H., Sengupta J., Valle M., Korostelev A., Eswar N., Stagg S.M., Van Roey P., Agrawal R.K., Harvey S.C., Sali A., Chapman M.S., Frank J.
Cell 113:789-801(2003) [PubMed: 12809609] [Abstract]
Cited for: STRUCTURE BY ELECTRON MICROSCOPY (11.50 ANGSTROMS).
Strain: MRE-600.
[8]"Structures of the bacterial ribosome at 3.5 A resolution."
Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W., Vila-Sanjurjo A., Holton J.M., Cate J.H.D.
Science 310:827-834(2005) [PubMed: 16272117] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.46 ANGSTROMS) OF 2 DIFFERENT RIBOSOME STRUCTURES.
Strain: MRE-600.
+Additional computationally mapped references.

Cross-references

Sequence databases

X01563 Genomic DNA. Translation: CAA25724.1.
U18997 Genomic DNA. Translation: AAA58098.1.
U00096 Genomic DNA. Translation: AAC76326.1.
AP009048 Genomic DNA. Translation: BAE77990.1.
PIRR5EC15. A02794.
RefSeqAP_004489.1.
NP_417760.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1P85electron microscopy12.30J1-144[»]
1P86electron microscopy11.50J1-144[»]
1VS6X-ray3.46L1-144[»]
1VS8X-ray3.46L1-144[»]
2AW4X-ray3.46L1-144[»]
2AWBX-ray3.46L1-144[»]
2GYAelectron microscopy2.00J4-143[»]
2GYCelectron microscopy2.00J4-143[»]
2I2TX-ray3.22L1-144[»]
2I2VX-ray3.22L1-144[»]
2J28electron microscopy8.00L1-144[»]
2QAMX-ray3.21L1-144[»]
2QAOX-ray3.21L1-144[»]
2QBAX-ray3.54L1-144[»]
2QBCX-ray3.54L1-144[»]
2QBEX-ray3.30L1-144[»]
2QBGX-ray3.30L1-144[»]
2QBIX-ray4.00L1-144[»]
2QBKX-ray4.00L1-144[»]
2QOVX-ray3.93L1-144[»]
2QOXX-ray3.93L1-144[»]
2QOZX-ray3.50L1-144[»]
2QP1X-ray3.50L1-144[»]
2RDOelectron microscopy9.10L1-144[»]
2VHMX-ray3.74L1-144[»]
2VHNX-ray3.74L1-144[»]
2Z4LX-ray4.45L1-144[»]
2Z4NX-ray4.45L1-144[»]
3BBXelectron microscopy10.00L1-144[»]
3DF2X-ray3.50L1-144[»]
3DF4X-ray3.50L1-144[»]
3FIKelectron microscopy-L2-144[»]
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:10752N.

Genome annotation databases

GeneID947798.
GenomeReviewsGene locus JW3263 in contig AP009048_GR.
Gene locus b3301 in contig U00096_GR.
KEGGecj:JW3263.
eco:b3301.

Organism-specific databases

EchoBASEEB0869.
EcoGeneEG10876. rplO.
CMRSearch...

Phylogenomic databases

HOGENOMP02413.
OMAP02413. SVKPGFE.

Enzyme and pathway databases

BioCycEcoCyc:EG10876-MON.

Family and domain databases

HAMAPMF_01341.
[Tree]
InterProIPR001196. Ribosomal_L15.
IPR005749. Ribosomal_L15_bac-type.
[Graphical view]
PANTHERPTHR12934. Ribosom_L15_bac. 1 hit.
PfamPF00256. L15. 1 hit.
PF01305. Ribosomal_L15. 1 hit.
[Graphical view]
TIGRFAMsTIGR01071. rplO_bact. 1 hit.
PROSITEPS00475. RIBOSOMAL_L15. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRL15_ECOLI
AccessionPrimary (citable) accession number: P02413
Secondary accession number(s): Q2M6W6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: June 16, 2009
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Ribosomal proteins

Ribosomal proteins families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents