Reviewed, UniProtKB/Swiss-Prot P02413 (RL15_ECOLI)

Names and origin

Protein names

Recommended name:
    50S ribosomal protein L15

Gene names

Name:	rplO
Ordered Locus Names:	b3301, JW3263

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	144 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This protein binds the 5S rRNA. It is required for the late stages of subunit assembly, and is essential for 5S rRNA assembly onto the ribosome. HAMAP MF_01341
Subunit structure	Part of the 50S ribosomal subunit. Contacts the 5S rRNA. Ref.5
Sequence similarities	Belongs to the ribosomal protein L15P family.
Mass spectrometry	Molecular mass is 14980.1 Da from positions 1 - 144. Determined by MALDI. Ref.6

Ontologies

Keywords
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Cellular component	large ribosomal subunit Inferred from electronic annotation. Source: InterPro
Molecular function	rRNA binding Inferred from electronic annotation. Source: HAMAP structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 144

144

50S ribosomal protein L15 HAMAP MF_01341

PRO_0000104721

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

144

Helix Strand Turn

Details...

Helix

33 – 35

3

Helix

39 – 41

3

Beta strand

42 – 45

4

Beta strand

47 – 49

3

Beta strand

83 – 86

4

Beta strand

97 – 99

3

Helix

129 – 132

4

Sequences

	Sequence		Length	Mass (Da)
	P02413-1 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 53D14CD948815FD9	FASTA	144	14,980
	10 20 30 40 50 60 MRLNTLSPAE GSKKAGKRLG RGIGSGLGKT GGRGHKGQKS RSGGGVRRGF EGGQMPLYRR 70 80 90 100 110 120 LPKFGFTSRK AAITAEIRLS DLAKVEGGVV DLNTLKAANI IGIQIEFAKV ILAGEVTTPV 130 140 TVRGLRVTKG ARAAIEAAGG KIEE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The primary structure of protein L15 located at the peptidyltransferase center of Escherichia coli ribosomes." Giorginis S., Chen R. FEBS Lett. 84:347-350(1977) [PubMed: 340263] [Abstract] Cited for: PROTEIN SEQUENCE. Strain: K.
[2]	"The spc ribosomal protein operon of Escherichia coli: sequence and cotranscription of the ribosomal protein genes and a protein export gene." Cerretti D.P., Dean D., Davis G.R., Bedwell D.M., Nomura M. Nucleic Acids Res. 11:2599-2616(1983) [PubMed: 6222285] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Incorporation of six additional proteins to complete the assembly map of the 50 S subunit from Escherichia coli ribosomes." Herold M., Nierhaus K.H. J. Biol. Chem. 262:8826-8833(1987) [PubMed: 3298242] [Abstract] Cited for: ASSEMBLY MAP OF THE 50S SUBUNIT. Strain: K12.
[6]	"Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry." Arnold R.J., Reilly J.P. Anal. Biochem. 269:105-112(1999) [PubMed: 10094780] [Abstract] Cited for: MASS SPECTROMETRY. Strain: K12 / ATCC 25404 / DSM 5698 / NCIMB 11290.
[7]	"Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement." Gao H., Sengupta J., Valle M., Korostelev A., Eswar N., Stagg S.M., Van Roey P., Agrawal R.K., Harvey S.C., Sali A., Chapman M.S., Frank J. Cell 113:789-801(2003) [PubMed: 12809609] [Abstract] Cited for: STRUCTURE BY ELECTRON MICROSCOPY (11.50 ANGSTROMS). Strain: MRE-600.
[8]	"Structures of the bacterial ribosome at 3.5 A resolution." Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W., Vila-Sanjurjo A., Holton J.M., Cate J.H.D. Science 310:827-834(2005) [PubMed: 16272117] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.46 ANGSTROMS) OF 2 DIFFERENT RIBOSOME STRUCTURES. Strain: MRE-600.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X01563 Genomic DNA. Translation: CAA25724.1.
U18997 Genomic DNA. Translation: AAA58098.1.
U00096 Genomic DNA. Translation: AAC76326.1.
AP009048 Genomic DNA. Translation: BAE77990.1.

PIR

R5EC15. A02794.

RefSeq

AP_004489.1.
NP_417760.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1P85	electron microscopy	12.30	J	1-144	[»]
1P86	electron microscopy	11.50	J	1-144	[»]
1VS6	X-ray	3.46	L	1-144	[»]
1VS8	X-ray	3.46	L	1-144	[»]
2AW4	X-ray	3.46	L	1-144	[»]
2AWB	X-ray	3.46	L	1-144	[»]
2GYA	electron microscopy	2.00	J	4-143	[»]
2GYC	electron microscopy	2.00	J	4-143	[»]
2I2T	X-ray	3.22	L	1-144	[»]
2I2V	X-ray	3.22	L	1-144	[»]
2J28	electron microscopy	8.00	L	1-144	[»]
2QAM	X-ray	3.21	L	1-144	[»]
2QAO	X-ray	3.21	L	1-144	[»]
2QBA	X-ray	3.54	L	1-144	[»]
2QBC	X-ray	3.54	L	1-144	[»]
2QBE	X-ray	3.30	L	1-144	[»]
2QBG	X-ray	3.30	L	1-144	[»]
2QBI	X-ray	4.00	L	1-144	[»]
2QBK	X-ray	4.00	L	1-144	[»]
2QOV	X-ray	3.93	L	1-144	[»]
2QOX	X-ray	3.93	L	1-144	[»]
2QOZ	X-ray	3.50	L	1-144	[»]
2QP1	X-ray	3.50	L	1-144	[»]
2RDO	electron microscopy	9.10	L	1-144	[»]
2VHM	X-ray	3.74	L	1-144	[»]
2VHN	X-ray	3.74	L	1-144	[»]
2Z4L	X-ray	4.45	L	1-144	[»]
2Z4N	X-ray	4.45	L	1-144	[»]
3BBX	electron microscopy	10.00	L	1-144	[»]
3DF2	X-ray	3.50	L	1-144	[»]
3DF4	X-ray	3.50	L	1-144	[»]
3FIK	electron microscopy	-	L	2-144	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:10752N.

Genome annotation databases

GeneID

947798.

GenomeReviews

Gene locus JW3263 in contig AP009048_GR.
Gene locus b3301 in contig U00096_GR.

KEGG

ecj:JW3263.
eco:b3301.

Organism-specific databases

EchoBASE

EB0869.

EcoGene

EG10876. rplO.

CMR

Search...

Phylogenomic databases

HOGENOM

P02413.

OMA

P02413. SVKPGFE.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10876-MON.

Family and domain databases

HAMAP

MF_01341.
[Tree]

InterPro

IPR001196. Ribosomal_L15.
IPR005749. Ribosomal_L15_bac-type.
[Graphical view]

PANTHER

PTHR12934. Ribosom_L15_bac. 1 hit.

Pfam

PF00256. L15. 1 hit.
PF01305. Ribosomal_L15. 1 hit.
[Graphical view]

TIGRFAMs

TIGR01071. rplO_bact. 1 hit.

PROSITE

PS00475. RIBOSOMAL_L15. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RL15_ECOLI

Accession

Primary (citable) accession number: P02413
Secondary accession number(s): Q2M6W6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	June 16, 2009
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)