60S ribosomal protein L28 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P02406 (RL28_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 116. History...

Clusters with 100%, 90%, 50% identity |

Documents (5) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
60S ribosomal protein L28

Alternative name(s):
L27a
L29
RP44
RP62
YL24

Gene names

Name:	RPL28
Synonyms:	CYH2
Ordered Locus Names:	YGL103W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	149 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.7
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ribosomal protein L15P family.

Ontologies

Keywords
Biological process	Antibiotic resistance Cycloheximide resistance
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translation Traceable author statement Ref.7. Source: SGD response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW response to cycloheximide Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytosolic large ribosomal subunit Traceable author statement Ref.7. Source: SGD nucleus Inferred from mutant phenotype Ref.6. Source: SGD
Molecular_function	RNA binding Inferred from direct assay PubMed 6337137. Source: SGD structural constituent of ribosome Traceable author statement Ref.7. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.8

Chain

2 – 149

148

60S ribosomal protein L28

PRO_0000104904

Regions

Motif

7 – 13

Nuclear localization signal Ref.6

Motif

24 – 30

Nuclear localization signal Ref.6

Natural variations

Natural variant

Q → E Confers resistance to cycloheximide, an inhibitor of polypeptide elongation. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P02406 [UniParc].

Last modified October 5, 2010. Version 3.
Checksum: B34C325C01E14ACB
Show »

FASTA

149

16,722

        10         20         30         40         50         60 
MPSRFTKTRK HRGHVSAGKG RIGKHRKHPG GRGMAGGQHH HRINMDKYHP GYFGKVGMRY 

        70         80         90        100        110        120 
FHKQQAHFWK PVLNLDKLWT LIPEDKRDQY LKSASKETAP VIDTLAAGYG KILGKGRIPN 

       130        140 
VPVIVKARFV SKLAEEKIRA AGGVVELIA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cycloheximide resistance in yeast: the gene and its protein." Kaufer N.F., Fried H.M., Schwindinger W.F., Jasin M., Warner J.R. Nucleic Acids Res. 11:3123-3135(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT GLU-38.
[2]	"Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII." Rieger M., Brueckner M., Schaefer M., Mueller-Auer S. Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Transcriptional elements of the yeast ribosomal protein gene CYH2." Schwindinger W.F., Warner J.R. J. Biol. Chem. 262:5690-5695(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-16.
[6]	"Characterization of nuclear localizing sequences derived from yeast ribosomal protein L29." Underwood M.R., Fried H.M. EMBO J. 9:91-99(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEAR LOCALIZATION SIGNAL.
[7]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[8]	"The action of N-terminal acetyltransferases on yeast ribosomal proteins." Arnold R.J., Polevoda B., Reilly J.P., Sherman F. J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract] Cited for: CLEAVAGE OF INITIATOR METHIONINE.
[9]	"Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions." Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J. Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 6-148, ELECTRON MICROSCOPY.
[10]	"Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation." Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J. EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING, ELECTRON MICROSCOPY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X01573 Genomic DNA. Translation: CAA25729.1.
Z72625 Genomic DNA. Translation: CAA96808.1.
M19490 Genomic DNA. Translation: AAA35002.1.
BK006941 Genomic DNA. Translation: DAA08004.1.

PIR

R6BY29. A02782.

RefSeq

NP_011412.1. NM_001180968.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K5Y	model	-	V	6-148	[»]
1S1I	electron microscopy	11.70	V	2-149	[»]
3IZS	electron microscopy	-	O	1-149	[»]
3O58	X-ray	4.00	Y	1-149	[»]
3O5H	X-ray	4.00	Y	1-149	[»]
3U5E	X-ray	3.00	a	1-149	[»]
3U5I	X-ray	3.00	a	1-149	[»]
4B6A	electron microscopy	8.10	a	1-149	[»]

ProteinModelPortal

P02406.

SMR

P02406. Positions 2-149.

ModBase

Search...

Protein-protein interaction databases

IntAct

P02406. 28 interactions.

MINT

MINT-1324980.

STRING

4932.YGL103W.

Proteomic databases

PaxDb

P02406.

PeptideAtlas

P02406.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YGL103W; YGL103W; YGL103W.

GeneID

852775.

KEGG

sce:YGL103W.

Organism-specific databases

SGD

S000003071. RPL28.

Phylogenomic databases

eggNOG

COG0200.

GeneTree

ENSGT00390000005534.

HOGENOM

HOG000231263.

K02900.

OMA

HHHRTNL.

OrthoDB

EOG47WRZ6.

Gene expression databases

Genevestigator

P02406.

GermOnline

YGL103W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR001196. Ribosomal_L15_CS.
IPR021131. Ribosomal_L18e/L15P.
[Graphical view]

Pfam

PF00828. Ribosomal_L18e. 1 hit.
[Graphical view]

SUPFAM

SSF52080. Ribosomal_L18e/L15. 1 hit.

PROSITE

PS00475. RIBOSOMAL_L15. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P02406.

NextBio

972248.

Entry information

Entry name

RL28_YEAST

Accession

Primary (citable) accession number: P02406
Secondary accession number(s): D6VU43

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	October 5, 2010
Last modified:	April 3, 2013
This is version 116 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents