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P02406

- RL28_YEAST

UniProt

P02406 - RL28_YEAST

Protein

60S ribosomal protein L28

Gene

RPL28

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 3 (05 Oct 2010)
      Previous versions | rss
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    Functioni

    GO - Molecular functioni

    1. RNA binding Source: SGD
    2. structural constituent of ribosome Source: SGD

    GO - Biological processi

    1. cytoplasmic translation Source: SGD
    2. response to antibiotic Source: UniProtKB-KW
    3. response to cycloheximide Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Keywords - Biological processi

    Antibiotic resistance, Cycloheximide resistance

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30602-MONOMER.
    ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    60S ribosomal protein L28
    Alternative name(s):
    L27a
    L29
    RP44
    RP62
    YL24
    Gene namesi
    Name:RPL28
    Synonyms:CYH2
    Ordered Locus Names:YGL103W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    SGDiS000003071. RPL28.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytosolic large ribosomal subunit Source: SGD
    2. nucleus Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 14914860S ribosomal protein L28PRO_0000104904Add
    BLAST

    Proteomic databases

    MaxQBiP02406.
    PaxDbiP02406.
    PeptideAtlasiP02406.

    Expressioni

    Gene expression databases

    GenevestigatoriP02406.

    Interactioni

    Subunit structurei

    Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

    Protein-protein interaction databases

    BioGridi33147. 120 interactions.
    IntActiP02406. 32 interactions.
    MINTiMINT-1324980.
    STRINGi4932.YGL103W.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1K5Ymodel-V6-148[»]
    1S1Ielectron microscopy11.70V2-149[»]
    1VW8electron microscopy6.10b1-149[»]
    1VWUelectron microscopy6.10b1-149[»]
    3IZSelectron microscopy-O1-149[»]
    3J65electron microscopy8.70N1-149[»]
    3O58X-ray4.00Y1-149[»]
    3O5HX-ray4.00Y1-149[»]
    3U5EX-ray3.00a1-149[»]
    3U5IX-ray3.00a1-149[»]
    4B6Aelectron microscopy8.10a1-149[»]
    4BYNelectron microscopy4.30a2-149[»]
    4BYUelectron microscopy6.60a2-149[»]
    4CUWelectron microscopy3.70a1-149[»]
    ProteinModelPortaliP02406.
    SMRiP02406. Positions 2-149.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP02406.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi7 – 137Nuclear localization signal1 Publication
    Motifi24 – 307Nuclear localization signal1 Publication

    Sequence similaritiesi

    Belongs to the ribosomal protein L15P family.Curated

    Phylogenomic databases

    eggNOGiCOG0200.
    GeneTreeiENSGT00390000005534.
    HOGENOMiHOG000231263.
    KOiK02900.
    OMAiLWTLIPE.
    OrthoDBiEOG7C5MN5.

    Family and domain databases

    HAMAPiMF_01341. Ribosomal_L15.
    InterProiIPR001196. Ribosomal_L15_CS.
    IPR021131. Ribosomal_L18e/L15P.
    [Graphical view]
    PfamiPF00828. Ribosomal_L18e. 1 hit.
    [Graphical view]
    SUPFAMiSSF52080. SSF52080. 1 hit.
    PROSITEiPS00475. RIBOSOMAL_L15. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P02406-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPSRFTKTRK HRGHVSAGKG RIGKHRKHPG GRGMAGGQHH HRINMDKYHP    50
    GYFGKVGMRY FHKQQAHFWK PVLNLDKLWT LIPEDKRDQY LKSASKETAP 100
    VIDTLAAGYG KILGKGRIPN VPVIVKARFV SKLAEEKIRA AGGVVELIA 149
    Length:149
    Mass (Da):16,722
    Last modified:October 5, 2010 - v3
    Checksum:iB34C325C01E14ACB
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti38 – 381Q → E Confers resistance to cycloheximide, an inhibitor of polypeptide elongation. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X01573 Genomic DNA. Translation: CAA25729.1.
    Z72625 Genomic DNA. Translation: CAA96808.1.
    M19490 Genomic DNA. Translation: AAA35002.1.
    BK006941 Genomic DNA. Translation: DAA08004.1.
    PIRiA02782. R6BY29.
    RefSeqiNP_011412.1. NM_001180968.1.

    Genome annotation databases

    EnsemblFungiiYGL103W; YGL103W; YGL103W.
    GeneIDi852775.
    KEGGisce:YGL103W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X01573 Genomic DNA. Translation: CAA25729.1 .
    Z72625 Genomic DNA. Translation: CAA96808.1 .
    M19490 Genomic DNA. Translation: AAA35002.1 .
    BK006941 Genomic DNA. Translation: DAA08004.1 .
    PIRi A02782. R6BY29.
    RefSeqi NP_011412.1. NM_001180968.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1K5Y model - V 6-148 [» ]
    1S1I electron microscopy 11.70 V 2-149 [» ]
    1VW8 electron microscopy 6.10 b 1-149 [» ]
    1VWU electron microscopy 6.10 b 1-149 [» ]
    3IZS electron microscopy - O 1-149 [» ]
    3J65 electron microscopy 8.70 N 1-149 [» ]
    3O58 X-ray 4.00 Y 1-149 [» ]
    3O5H X-ray 4.00 Y 1-149 [» ]
    3U5E X-ray 3.00 a 1-149 [» ]
    3U5I X-ray 3.00 a 1-149 [» ]
    4B6A electron microscopy 8.10 a 1-149 [» ]
    4BYN electron microscopy 4.30 a 2-149 [» ]
    4BYU electron microscopy 6.60 a 2-149 [» ]
    4CUW electron microscopy 3.70 a 1-149 [» ]
    ProteinModelPortali P02406.
    SMRi P02406. Positions 2-149.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33147. 120 interactions.
    IntActi P02406. 32 interactions.
    MINTi MINT-1324980.
    STRINGi 4932.YGL103W.

    Proteomic databases

    MaxQBi P02406.
    PaxDbi P02406.
    PeptideAtlasi P02406.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGL103W ; YGL103W ; YGL103W .
    GeneIDi 852775.
    KEGGi sce:YGL103W.

    Organism-specific databases

    SGDi S000003071. RPL28.

    Phylogenomic databases

    eggNOGi COG0200.
    GeneTreei ENSGT00390000005534.
    HOGENOMi HOG000231263.
    KOi K02900.
    OMAi LWTLIPE.
    OrthoDBi EOG7C5MN5.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30602-MONOMER.
    Reactomei REACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Miscellaneous databases

    EvolutionaryTracei P02406.
    NextBioi 972248.
    PROi P02406.

    Gene expression databases

    Genevestigatori P02406.

    Family and domain databases

    HAMAPi MF_01341. Ribosomal_L15.
    InterProi IPR001196. Ribosomal_L15_CS.
    IPR021131. Ribosomal_L18e/L15P.
    [Graphical view ]
    Pfami PF00828. Ribosomal_L18e. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52080. SSF52080. 1 hit.
    PROSITEi PS00475. RIBOSOMAL_L15. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cycloheximide resistance in yeast: the gene and its protein."
      Kaufer N.F., Fried H.M., Schwindinger W.F., Jasin M., Warner J.R.
      Nucleic Acids Res. 11:3123-3135(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT GLU-38.
    2. "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
      Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
      Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Transcriptional elements of the yeast ribosomal protein gene CYH2."
      Schwindinger W.F., Warner J.R.
      J. Biol. Chem. 262:5690-5695(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-16.
    6. "Characterization of nuclear localizing sequences derived from yeast ribosomal protein L29."
      Underwood M.R., Fried H.M.
      EMBO J. 9:91-99(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEAR LOCALIZATION SIGNAL.
    7. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
      Planta R.J., Mager W.H.
      Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE, SUBUNIT.
    8. "The action of N-terminal acetyltransferases on yeast ribosomal proteins."
      Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
      J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: CLEAVAGE OF INITIATOR METHIONINE.
    9. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions."
      Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J.
      Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING OF 6-148, ELECTRON MICROSCOPY.
    11. "Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation."
      Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J.
      EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING, ELECTRON MICROSCOPY.
    12. "Crystal structure of the eukaryotic ribosome."
      Ben-Shem A., Jenner L., Yusupova G., Yusupov M.
      Science 330:1203-1209(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (4.0 ANGSTROMS) OF 80S RIBOSOME.
    13. Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 80S RIBOSOME.

    Entry informationi

    Entry nameiRL28_YEAST
    AccessioniPrimary (citable) accession number: P02406
    Secondary accession number(s): D6VU43
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: October 5, 2010
    Last modified: October 1, 2014
    This is version 125 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Ribosomal proteins
      Ribosomal proteins families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3