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Protein

60S acidic ribosomal protein P2-beta

Gene

RPP2B

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in the elongation step of protein synthesis.

GO - Molecular functioni

  • large ribosomal subunit rRNA binding Source: GO_Central
  • protein kinase activator activity Source: SGD
  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • positive regulation of protein kinase activity Source: SGD
  • translational elongation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-29930-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
60S acidic ribosomal protein P2-beta
Short name:
P2B
Alternative name(s):
L12EIA
L45
YL44C
YP2beta
YPA1
Gene namesi
Name:RPP2B
Synonyms:L12EIA, RPL45, RPLA4
Ordered Locus Names:YDR382W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR382W.
SGDiS000002790. RPP2B.

Subcellular locationi

GO - Cellular componenti

  • cytosolic large ribosomal subunit Source: SGD
  • preribosome, large subunit precursor Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11011060S acidic ribosomal protein P2-betaPRO_0000157682Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei29 – 291PhosphoserineCombined sources
Cross-linki49 – 49Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei100 – 1001PhosphoserineCombined sources

Post-translational modificationi

The N-terminus is not modified.

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP02400.
TopDownProteomicsiP02400.

PTM databases

iPTMnetiP02400.

Interactioni

Subunit structurei

Heterodimer of P1A-P2B. Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). The 5 acidic ribosomal P-proteins form the stalk structure of the 60S subunit. They are organized as a pentameric complex in which P0 interacts with 2 heterodimers, P1A-P2B and P1B-P2A.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RPP1AP053182EBI-15464,EBI-15452

Protein-protein interaction databases

BioGridi32443. 153 interactions.
DIPiDIP-580N.
IntActiP02400. 31 interactions.
MINTiMINT-411356.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3N2DX-ray2.22B100-105[»]
3N3XX-ray1.70B100-105[»]
DisProtiDP00002.
ProteinModelPortaliP02400.
SMRiP02400. Positions 1-70.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP02400.

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L12P family.Curated

Phylogenomic databases

GeneTreeiENSGT00550000074828.
HOGENOMiHOG000229897.
InParanoidiP02400.
KOiK02943.
OMAiNDPSVED.
OrthoDBiEOG092C5X8C.

Family and domain databases

HAMAPiMF_01478. Ribosomal_L12_arch. 1 hit.
InterProiIPR027534. Ribosomal_L12.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P02400-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKYLAAYLLL VQGGNAAPSA ADIKAVVESV GAEVDEARIN ELLSSLEGKG
60 70 80 90 100
SLEEIIAEGQ KKFATVPTGG ASSAAAGAAG AAAGGDAAEE EKEEEAKEES
110
DDDMGFGLFD
Length:110
Mass (Da):11,050
Last modified:July 1, 1989 - v2
Checksum:iEC45406CB5F199F4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti75 – 784AAGA → GPAS AA sequence (PubMed:7030402).Curated
Sequence conflicti86 – 872DA → GD AA sequence (PubMed:7030402).Curated
Sequence conflicti89 – 891E → A AA sequence (PubMed:7030402).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03761 Genomic DNA. Translation: AAA34972.1.
M26505 Genomic DNA. Translation: AAA34732.1.
U28373 Genomic DNA. Translation: AAB64818.1.
U32274 Genomic DNA. Translation: AAB64824.1.
BK006938 Genomic DNA. Translation: DAA12226.1.
PIRiA35109. R5BYA1.
RefSeqiNP_010670.3. NM_001180690.3.

Genome annotation databases

EnsemblFungiiYDR382W; YDR382W; YDR382W.
GeneIDi851990.
KEGGisce:YDR382W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03761 Genomic DNA. Translation: AAA34972.1.
M26505 Genomic DNA. Translation: AAA34732.1.
U28373 Genomic DNA. Translation: AAB64818.1.
U32274 Genomic DNA. Translation: AAB64824.1.
BK006938 Genomic DNA. Translation: DAA12226.1.
PIRiA35109. R5BYA1.
RefSeqiNP_010670.3. NM_001180690.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3N2DX-ray2.22B100-105[»]
3N3XX-ray1.70B100-105[»]
DisProtiDP00002.
ProteinModelPortaliP02400.
SMRiP02400. Positions 1-70.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32443. 153 interactions.
DIPiDIP-580N.
IntActiP02400. 31 interactions.
MINTiMINT-411356.

PTM databases

iPTMnetiP02400.

Proteomic databases

MaxQBiP02400.
TopDownProteomicsiP02400.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR382W; YDR382W; YDR382W.
GeneIDi851990.
KEGGisce:YDR382W.

Organism-specific databases

EuPathDBiFungiDB:YDR382W.
SGDiS000002790. RPP2B.

Phylogenomic databases

GeneTreeiENSGT00550000074828.
HOGENOMiHOG000229897.
InParanoidiP02400.
KOiK02943.
OMAiNDPSVED.
OrthoDBiEOG092C5X8C.

Enzyme and pathway databases

BioCyciYEAST:G3O-29930-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

EvolutionaryTraceiP02400.
PROiP02400.

Family and domain databases

HAMAPiMF_01478. Ribosomal_L12_arch. 1 hit.
InterProiIPR027534. Ribosomal_L12.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRLA4_YEAST
AccessioniPrimary (citable) accession number: P02400
Secondary accession number(s): D6VT16
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 1, 1989
Last modified: September 7, 2016
This is version 166 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Yeasts contain 4 individual small ribosomal A proteins (RPA) which can be classified into two couples of similar but not identical sequences. Each couple is distinctly related to one of the two A proteins present in multicellular organisms.
Present with 602000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.