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P02400

- RLA4_YEAST

UniProt

P02400 - RLA4_YEAST

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Protein
60S acidic ribosomal protein P2-beta
Gene
RPP2B, L12EIA, RPL45, RPLA4, YDR382W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Plays an important role in the elongation step of protein synthesis.UniRule annotation

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. protein kinase activator activity Source: SGD
  3. structural constituent of ribosome Source: SGD

GO - Biological processi

  1. cytoplasmic translation Source: SGD
  2. positive regulation of protein kinase activity Source: SGD
  3. translational elongation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-29930-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
60S acidic ribosomal protein P2-beta
Short name:
P2B
Alternative name(s):
L12EIA
L45
YL44C
YP2beta
YPA1
Gene namesi
Name:RPP2B
Synonyms:L12EIA, RPL45, RPLA4
Ordered Locus Names:YDR382W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

CYGDiYDR382w.
SGDiS000002790. RPP2B.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytosolic large ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11011060S acidic ribosomal protein P2-betaUniRule annotation
PRO_0000157682Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei29 – 291Phosphoserine1 Publication
Modified residuei100 – 1001Phosphoserine3 Publications

Post-translational modificationi

The N-terminus is not modified.UniRule annotation

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP02400.
PaxDbiP02400.
PeptideAtlasiP02400.

Expressioni

Gene expression databases

GenevestigatoriP02400.

Interactioni

Subunit structurei

Heterodimer of P1A-P2B. Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). The 5 acidic ribosomal P-proteins form the stalk structure of the 60S subunit. They are organized as a pentameric complex in which P0 interacts with 2 heterodimers, P1A-P2B and P1B-P2A.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RPP1AP053182EBI-15464,EBI-15452

Protein-protein interaction databases

BioGridi32443. 203 interactions.
DIPiDIP-580N.
IntActiP02400. 31 interactions.
MINTiMINT-411356.
STRINGi4932.YDR382W.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3N2DX-ray2.22B100-105[»]
3N3XX-ray1.70B100-105[»]
DisProtiDP00002.
ProteinModelPortaliP02400.
SMRiP02400. Positions 1-70.

Miscellaneous databases

EvolutionaryTraceiP02400.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2058.
GeneTreeiENSGT00550000074828.
HOGENOMiHOG000229897.
KOiK02943.
OMAiWTEEQHA.
OrthoDBiEOG708WC9.

Family and domain databases

HAMAPiMF_01478. Ribosomal_L12_arch.
InterProiIPR001813. Ribosomal_L10/L12.
IPR027534. Ribosomal_L12.
[Graphical view]
PfamiPF00428. Ribosomal_60s. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P02400-1 [UniParc]FASTAAdd to Basket

« Hide

MKYLAAYLLL VQGGNAAPSA ADIKAVVESV GAEVDEARIN ELLSSLEGKG    50
SLEEIIAEGQ KKFATVPTGG ASSAAAGAAG AAAGGDAAEE EKEEEAKEES 100
DDDMGFGLFD 110
Length:110
Mass (Da):11,050
Last modified:July 1, 1989 - v2
Checksum:iEC45406CB5F199F4
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti75 – 784AAGA → GPAS AA sequence 1 Publication
Sequence conflicti86 – 872DA → GD AA sequence 1 Publication
Sequence conflicti89 – 891E → A AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J03761 Genomic DNA. Translation: AAA34972.1.
M26505 Genomic DNA. Translation: AAA34732.1.
U28373 Genomic DNA. Translation: AAB64818.1.
U32274 Genomic DNA. Translation: AAB64824.1.
BK006938 Genomic DNA. Translation: DAA12226.1.
PIRiA35109. R5BYA1.
RefSeqiNP_010670.3. NM_001180690.3.

Genome annotation databases

EnsemblFungiiYDR382W; YDR382W; YDR382W.
GeneIDi851990.
KEGGisce:YDR382W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J03761 Genomic DNA. Translation: AAA34972.1 .
M26505 Genomic DNA. Translation: AAA34732.1 .
U28373 Genomic DNA. Translation: AAB64818.1 .
U32274 Genomic DNA. Translation: AAB64824.1 .
BK006938 Genomic DNA. Translation: DAA12226.1 .
PIRi A35109. R5BYA1.
RefSeqi NP_010670.3. NM_001180690.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3N2D X-ray 2.22 B 100-105 [» ]
3N3X X-ray 1.70 B 100-105 [» ]
DisProti DP00002.
ProteinModelPortali P02400.
SMRi P02400. Positions 1-70.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32443. 203 interactions.
DIPi DIP-580N.
IntActi P02400. 31 interactions.
MINTi MINT-411356.
STRINGi 4932.YDR382W.

Proteomic databases

MaxQBi P02400.
PaxDbi P02400.
PeptideAtlasi P02400.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDR382W ; YDR382W ; YDR382W .
GeneIDi 851990.
KEGGi sce:YDR382W.

Organism-specific databases

CYGDi YDR382w.
SGDi S000002790. RPP2B.

Phylogenomic databases

eggNOGi COG2058.
GeneTreei ENSGT00550000074828.
HOGENOMi HOG000229897.
KOi K02943.
OMAi WTEEQHA.
OrthoDBi EOG708WC9.

Enzyme and pathway databases

BioCyci YEAST:G3O-29930-MONOMER.

Miscellaneous databases

EvolutionaryTracei P02400.
NextBioi 970154.

Gene expression databases

Genevestigatori P02400.

Family and domain databases

HAMAPi MF_01478. Ribosomal_L12_arch.
InterProi IPR001813. Ribosomal_L10/L12.
IPR027534. Ribosomal_L12.
[Graphical view ]
Pfami PF00428. Ribosomal_60s. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Independent genes coding for three acidic proteins of the large ribosomal subunit from Saccharomyces cerevisiae."
    Remacha M., Saenz-Robles M.T., Vilella M.D., Ballesta J.P.G.
    J. Biol. Chem. 263:9094-9101(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "A family of genes encode the multiple forms of the Saccharomyces cerevisiae ribosomal proteins equivalent to the Escherichia coli L12 protein and a single form of the L10-equivalent ribosomal protein."
    Newton C.H., Shimmin L.C., Yee J., Dennis P.P.
    J. Bacteriol. 172:579-588(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: SR26-12C.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Primary structure of an acidic ribosomal protein YPA1 from Saccharomyces cerevisiae. Isolation and characterization of peptides and the complete amino acid sequence."
    Itoh T.
    Biochim. Biophys. Acta 671:16-24(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.
  6. "The acidic phosphoproteins from Saccharomyces cerevisiae ribosomes. NH2-terminal acetylation is a conserved difference between P1 and P2 proteins."
    Santos C., Ortiz-Reyes B., Naranda T., Remacha M., Ballesta J.P.G.
    Biochemistry 32:4231-4236(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-6.
  7. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
    Planta R.J., Mager W.H.
    Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE, SUBUNIT.
  8. "The action of N-terminal acetyltransferases on yeast ribosomal proteins."
    Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
    J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: ANALYSIS OF N-TERMINUS.
  9. "Asymmetric interactions between the acidic P1 and P2 proteins in the Saccharomyces cerevisiae ribosomal stalk."
    Guarinos E., Remacha M., Ballesta J.P.G.
    J. Biol. Chem. 276:32474-32479(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RPP1A.
  10. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  11. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  12. "Yeast ribosomal P0 protein has two separate binding sites for P1/P2 proteins."
    Krokowski D., Boguszewska A., Abramczyk D., Liljas A., Tchorzewski M., Grankowski N.
    Mol. Microbiol. 60:386-400(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RPP0 AND RPP1A.
  13. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-100, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  14. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-29, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  15. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-100, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  16. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-100, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  17. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRLA4_YEAST
AccessioniPrimary (citable) accession number: P02400
Secondary accession number(s): D6VT16
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 1, 1989
Last modified: June 11, 2014
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Yeasts contain 4 individual small ribosomal A proteins (RPA) which can be classified into two couples of similar but not identical sequences. Each couple is distinctly related to one of the two A proteins present in multicellular organisms.
Present with 602000 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

Similar proteinsi