P02391 (RL6_GEOSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
50S ribosomal protein L6

Alternative name(s):
BL10

Gene names

Name:

rplF

Organism

Geobacillus stearothermophilus (Bacillus stearothermophilus)

Taxonomic identifier

1422 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	178 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center By similarity. This protein binds to the 23S rRNA, and is important in its secondary structure. HAMAP-Rule MF_01365
Subunit structure	Part of the 50S ribosomal subunit.
Sequence similarities	Belongs to the ribosomal protein L6P family.

Ontologies

Keywords
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	translation Inferred from electronic annotation. Source: HAMAP
Cellular_component	ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	rRNA binding Inferred from electronic annotation. Source: HAMAP structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.2

Chain

2 – 178

177

50S ribosomal protein L6 HAMAP-Rule MF_01365

PRO_0000131037

Secondary structure

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178

Helix Strand Turn

Details...

Beta strand

16 – 20

5

Beta strand

23 – 28

6

Beta strand

31 – 36

6

Beta strand

42 – 46

5

Beta strand

49 – 53

5

Helix

59 – 80

22

Beta strand

83 – 91

9

Beta strand

95 – 99

5

Beta strand

102 – 111

10

Beta strand

113 – 115

3

Beta strand

121 – 127

7

Beta strand

130 – 136

7

Helix

138 – 149

12

Turn

156 – 158

3

Beta strand

161 – 164

4

Sequences

	Sequence		Length	Mass (Da)
	P02391 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: C9D83BA8F35DB4FA	FASTA	178	19,299
	10 20 30 40 50 60 MSRVGKKPIE IPAGVTVTVN GNTVTVKGPK GELTRTFHPD MTITVEGNVI TVTRPSDEKH 70 80 90 100 110 120 HRALHGTTRS LLANMVEGVS KGYEKALELV GVGYRASKQG KKLVLSVGYS HPVEIEPEEG 130 140 150 160 170 LEIEVPSQTK IIVKGADKQR VGELAANIRA VRPPEPYKGK GIRYEGELVR LKEGKTGK

References

[1]	"Cloning, sequencing, and overexpression of genes for ribosomal proteins from Bacillus stearothermophilus." Ramakrishnan V., Gerchman S.E. J. Biol. Chem. 266:880-885(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The amino acid sequence of protein BL10 from the 50S subunit of the Bacillus stearothermophilus ribosome." Kimura M., Rawlings N., Appelt K. FEBS Lett. 136:58-64(1981) Cited for: PROTEIN SEQUENCE OF 2-178.
[3]	"Protein-rRNA binding features and their structural and functional implications in ribosomes as determined by cross-linking studies." Urlaub H., Kruft V., Bischof O., Mueller E.-C., Wittmann-Liebold B. EMBO J. 14:4578-4588(1995) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 150-164, CROSS-LINKING TO RRNA. Strain: 799.
[4]	"Ribosomal protein L6: structural evidence of gene duplication from a primitive RNA binding protein." Golden B.L., Ramakrishnan V., White S.W. EMBO J. 12:4901-4908(1993) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS).
[5]	"Ribosomal proteins S5 and L6: high-resolution crystal structures and roles in protein synthesis and antibiotic resistance." Davies C., Bussiere D.E., Golden B.L., Porter S.J., Ramakrishnan V., White S.W. J. Mol. Biol. 279:873-888(1998) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
[6]	"Placement of protein and RNA structures into a 5 A-resolution map of the 50S ribosomal subunit." Ban N., Nissen P., Hansen J., Capel M., Moore P.B., Steitz T.A. Nature 400:841-847(1999) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING ONTO THE H.MARISMORTUI 50S RIBOSOME.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M57622 Genomic DNA. Translation: AAA22700.1. Sequence problems.

PIR

R5BS0F. A02766.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1RL6	X-ray	2.00	A	2-178	[»]

ProteinModelPortal

P02391.

SMR

P02391. Positions 8-171.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

3.90.930.12. 2 hits.

HAMAP

MF_01365_B. Ribosomal_L6_B.

InterPro

IPR000702. Ribosomal_L6.
IPR020040. Ribosomal_L6_a/b-dom.
IPR019906. Ribosomal_L6_bac-type.
IPR002358. Ribosomal_L6_CS.
[Graphical view]

PANTHER

PTHR11655. PTHR11655. 1 hit.
PTHR11655:SF6. PTHR11655:SF6. 1 hit.

Pfam

PF00347. Ribosomal_L6. 2 hits.
[Graphical view]

PIRSF

PIRSF002162. Ribosomal_L6. 1 hit.

PRINTS

PR00059. RIBOSOMALL6.

SUPFAM

SSF56053. Ribosomal_L6. 2 hits.

TIGRFAMs

TIGR03654. L6_bact. 1 hit.

PROSITE

PS00525. RIBOSOMAL_L6_1. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P02391.

Entry information

Entry name

RL6_GEOSE

Accession

Primary (citable) accession number: P02391

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program