30S ribosomal protein S7 - Escherichia coli (strain K12)

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Reviewed, UniProtKB/Swiss-Prot P02359 (RS7_ECOLI)

Last modified June 16, 2009. Version 116. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
30S ribosomal protein S7

Gene names

Name:	rpsG
Ordered Locus Names:	b3341, JW3303

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length	179 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center, where it has been shown to contact mRNA. Has been shown to contact tRNA in both the P and E sites; it probably blocks exit of the E site tRNA. Ref.11 Protein S7 is also a translational repressor protein; it regulates the expression of the str operon members to different degrees by binding to its mRNA. Ref.11
Subunit structure	Part of the 30S ribosomal subunit. Contacts proteins S9 and S11 By similarity. Cross-links to IF3 and the P and E site tRNAs.
Miscellaneous	The strain K12 sequence is shown. HAMAP MF_00480 Has been predicted to contact the N-terminal domain of IF-3 based on footprint studies (Ref.18); exactly how IF-3 interacts with the 30S subunit is controversial. HAMAP MF_00480
Sequence similarities	Belongs to the ribosomal protein S7P family.
Mass spectrometry	Molecular mass is 19888.7 Da from positions 2 - 179. Determined by MALDI. Ref.17

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Ontologies

Keywords
Ligand	RNA-binding rRNA-binding tRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Cellular component	membrane Inferred from direct assay. Source: UniProtKB small ribosomal subunit Inferred from electronic annotation. Source: InterPro
Molecular function	rRNA binding Inferred from electronic annotation. Source: HAMAP structural constituent of ribosome Inferred from electronic annotation. Source: InterPro tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.3

Chain

2 – 179

178

30S ribosomal protein S7 HAMAP MF_00480

PRO_0000124259

Natural variations

Natural variant

157 – 179

Missing in strain: B and L44. HAMAP MF_00480

Experimental info

Mutagenesis

2 – 18

Missing: Defective in ribosome assembly; accumulates to abnormally high levels on polysomes; significantly decreases affinity for its own mRNA. Ref.15 Ref.16

Mutagenesis

K → A or E: Defective in ribosome assembly. Ref.15 Ref.16

Mutagenesis

116

M → G: Significantly decreases affinity for its own mRNA. Ref.15 Ref.16

Sequence conflict

Missing AA sequence Ref.3

Secondary structure

179

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P02359-1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 8627DB380C4A9C0D
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FASTA

179

20,019

        10         20         30         40         50         60 
MPRRRVIGQR KILPDPKFGS ELLAKFVNIL MVDGKKSTAE SIVYSALETL AQRSGKSELE 

        70         80         90        100        110        120 
AFEVALENVR PTVEVKSRRV GGSTYQVPVE VRPVRRNALA MRWIVEAARK RGDKSMALRL 

       130        140        150        160        170 
ANELSDAAEN KGTAVKKRED VHRMAEANKA FAHYRWLSLR SFSHQAGASS KQPALGYLN

« Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The primary structure of ribosomal protein S7 from E. coli strains K and B." Reinbolt J., Tritsch D., Wittmann-Liebold B. Biochimie 61:501-522(1979) [PubMed: 385062] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-179. Strain: B and K.
[4]	"DNA sequences from the str operon of Escherichia coli." Post L.E., Nomura M. J. Biol. Chem. 255:4660-4666(1980) [PubMed: 6989816] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-81 AND 148-179. Strain: K12.
[5]	"Mutant sequences in the rpsL gene of Escherichia coli B/r: mechanistic implications for spontaneous and ultraviolet light mutagenesis." Timms A.R., Steingrimsdottir H., Lehmann A.R., Bridges B.A. Mol. Gen. Genet. 232:89-96(1992) [PubMed: 1552908] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-81. Strain: B/R.
[6]	"Comparison of the complete sequence of the str operon in Salmonella typhimurium and Escherichia coli." Johanson U., Hughes D. Gene 120:93-98(1992) [PubMed: 1398129] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 81-173. Strain: K12 / MG1655 / ATCC 47076.
[7]	Weigel C.T.O. Submitted (MAY-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 147-156. Strain: L44.
[8]	"Protein-rRNA binding features and their structural and functional implications in ribosomes as determined by cross-linking studies." Urlaub H., Kruft V., Bischof O., Mueller E.-C., Wittmann-Liebold B. EMBO J. 14:4578-4588(1995) [PubMed: 7556101] [Abstract] Cited for: PROTEIN SEQUENCE OF 111-131, CROSS-LINKING TO RRNA. Strain: MRE-600.
[9]	"Photochemical cross-linking of initiation factor-3 to Escherichia coli 30 S ribosomal subunits." MacKeen L.A., Kahan L., Wahba A.J., Schwartz I. J. Biol. Chem. 255:10526-10531(1980) [PubMed: 7000779] [Abstract] Cited for: CROSS-LINKING TO IF3. Strain: B.
[10]	"Direct cross-links between initiation factors 1, 2, and 3 and ribosomal proteins promoted by 2-iminothiolane." Boileau G., Butler P., Hershey J.W.B., Traut R.R. Biochemistry 22:3162-3170(1983) [PubMed: 6349681] [Abstract] Cited for: CROSS-LINKING TO IF3. Strain: MRE-600.
[11]	"Assembly of the 30S subunit from Escherichia coli ribosomes occurs via two assembly domains which are initiated by S4 and S7." Nowotny V., Nierhaus K.H. Biochemistry 27:7051-7055(1988) [PubMed: 2461734] [Abstract] Cited for: ROLE IN SUBUNIT ASSEMBLY. Strain: K12 / D10.
[12]	"Post-transcriptional regulation of the str operon in Escherichia coli. Ribosomal protein S7 inhibits coupled translation of S7 but not its independent translation." Saito K., Mattheakis L.C., Nomura M. J. Mol. Biol. 235:111-124(1994) [PubMed: 7507167] [Abstract] Cited for: MECHANISM OF TRANSLATION REGULATION. Strain: K12.
[13]	"The ribosomal neighbourhood of the central fold of tRNA: cross-links from position 47 of tRNA located at the A, P or E site." Osswald M., Doering T., Brimacombe R. Nucleic Acids Res. 23:4635-4641(1995) [PubMed: 8524654] [Abstract] Cited for: CROSS-LINKING TO THE TRNA CENTRAL FOLD. Strain: MRE-600.
[14]	"The cross-link from the upstream region of mRNA to ribosomal protein S7 is located in the C-terminal peptide: experimental verification of a prediction from modeling studies." Greuer B., Thiede B., Brimacombe R. RNA 5:1521-1525(1999) [PubMed: 10606263] [Abstract] Cited for: CROSS-LINKING TO MRNA. Strain: B and K.
[15]	"Tagging ribosomal protein S7 allows rapid identification of mutants defective in assembly and function of 30S subunits." Fredrick K., Dunny G.M., Noller H.F. J. Mol. Biol. 298:379-394(2000) [PubMed: 10772857] [Abstract] Cited for: MUTAGENESIS OF CONSERVED SURFACE RESIDUES. Strain: K.
[16]	"Ribosomal protein S7 from Escherichia coli uses the same determinants to bind 16S ribosomal RNA and its messenger RNA." Robert F., Brakier-Gingras L. Nucleic Acids Res. 29:677-682(2001) [PubMed: 11160889] [Abstract] Cited for: MUTAGENESIS OF CONSERVED RESIDUES. Strain: K12.
[17]	"Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry." Arnold R.J., Reilly J.P. Anal. Biochem. 269:105-112(1999) [PubMed: 10094780] [Abstract] Cited for: MASS SPECTROMETRY. Strain: K12 / ATCC 25404 / DSM 5698 / NCIMB 11290.
[18]	"Interaction of translation initiation factor 3 with the 30S ribosomal subunit." Dallas A., Noller H.F. Mol. Cell 8:855-864(2001) [PubMed: 11684020] [Abstract] Cited for: MODELING OF IF-3/30S SUBUNIT INTERACTION.
[19]	"All-atom homology model of the Escherichia coli 30S ribosomal subunit." Tung C.-S., Joseph S., Sanbonmatsu K.Y. Nat. Struct. Biol. 9:750-755(2002) [PubMed: 12244297] [Abstract] Cited for: 3D-STRUCTURE MODELING.
[20]	"Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement." Gao H., Sengupta J., Valle M., Korostelev A., Eswar N., Stagg S.M., Van Roey P., Agrawal R.K., Harvey S.C., Sali A., Chapman M.S., Frank J. Cell 113:789-801(2003) [PubMed: 12809609] [Abstract] Cited for: STRUCTURE BY ELECTRON MICROSCOPY (11.50 ANGSTROMS). Strain: MRE-600.
[21]	"Structures of the bacterial ribosome at 3.5 A resolution." Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W., Vila-Sanjurjo A., Holton J.M., Cate J.H.D. Science 310:827-834(2005) [PubMed: 16272117] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.46 ANGSTROMS) OF 2 DIFFERENT RIBOSOME STRUCTURES. Strain: MRE-600.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

U18997 Genomic DNA. Translation: AAA58138.1.
U00096 Genomic DNA. Translation: AAC76366.1.
AP009048 Genomic DNA. Translation: BAE77950.1.
V00355 Genomic DNA. Translation: CAA23649.1.
J01689 Genomic DNA. Translation: AAA50990.1.
X64592 Genomic DNA. Translation: CAA45881.1.
X65735 Genomic DNA. Translation: CAA46644.1.
J01688 Genomic DNA. Translation: AAA50989.1.

PIR

R3EC7K. H65127.

RefSeq

AP_004449.1.
NP_417800.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1M5G	model	-	G	3-156	[»]
1P6G	electron microscopy	12.30	G	2-179	[»]
1P87	electron microscopy	11.50	G	2-179	[»]
1VS5	X-ray	3.46	G	1-179	[»]
1VS7	X-ray	3.46	G	1-179	[»]
2AVY	X-ray	3.46	G	2-179	[»]
2AW7	X-ray	3.46	G	2-179	[»]
2GY9	electron microscopy	-	G	20-155	[»]
2GYB	electron microscopy	-	G	20-155	[»]
2I2P	X-ray	3.22	G	2-178	[»]
2I2U	X-ray	3.22	G	2-178	[»]
2QAL	X-ray	3.21	G	2-179	[»]
2QAN	X-ray	3.21	G	2-179	[»]
2QB9	X-ray	3.54	G	2-179	[»]
2QBB	X-ray	3.54	G	2-179	[»]
2QBD	X-ray	3.30	G	2-179	[»]
2QBF	X-ray	3.30	G	2-179	[»]
2QBH	X-ray	4.00	G	2-179	[»]
2QBJ	X-ray	4.00	G	2-179	[»]
2QOU	X-ray	3.93	G	2-179	[»]
2QOW	X-ray	3.93	G	2-179	[»]
2QOY	X-ray	3.50	G	2-179	[»]
2QP0	X-ray	3.50	G	2-179	[»]
2VHO	X-ray	3.74	G	2-179	[»]
2VHP	X-ray	3.74	G	2-179	[»]
2Z4K	X-ray	4.45	G	2-179	[»]
2Z4M	X-ray	4.45	G	2-179	[»]
3DF1	X-ray	3.50	G	2-178	[»]
3DF3	X-ray	3.50	G	2-178	[»]
3FIH	electron microscopy	-	G	3-152	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:10783N.

IntAct

P02359. 150 interactions.

PTM databases

PhosSite

P02359.

Genome annotation databases

GeneID

947846.

GenomeReviews

Gene locus JW3303 in contig AP009048_GR.
Gene locus b3341 in contig U00096_GR.

KEGG

ecj:JW3303.
eco:b3341.

Organism-specific databases

EchoBASE

EB0899.

EcoGene

EG10906. rpsG.

CMR

Search...

Phylogenomic databases

HOGENOM

P02359.

OMA

P02359. MAESNKA.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10906-MON.

Family and domain databases

HAMAP

MF_00480.
[Tree]

InterPro

IPR000235. Ribosomal_S7.
IPR005717. Ribosomal_S7_bac-type.
[Graphical view]

Gene3D

G3DSA:1.10.455.10. Ribosomal_S7. 1 hit.

PANTHER

PTHR11205. Ribosomal_S7. 1 hit.

Pfam

PF00177. Ribosomal_S7. 1 hit.
[Graphical view]

ProDom

PD000817. Ribosomal_S7. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR01029. rpsG_bact. 1 hit.

PROSITE

PS00052. RIBOSOMAL_S7. 1 hit.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

RS7_ECOLI

Accession

Primary (citable) accession number: P02359
Secondary accession number(s): Q2M706

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 116 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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