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Reviewed, UniProtKB/Swiss-Prot P02358 (RS6_ECOLI)

Last modified June 16, 2009. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    30S ribosomal protein S6
Cleaved into the following 2 chains:
    1- Recommended name:
            30S ribosomal protein S6, fully modified isoform
    2- Recommended name:
            30S ribosomal protein S6, non-modified isoform
Gene names
Name: rpsF
Ordered Locus Names: b4200, JW4158
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length135 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Binds together with S18 to 16S ribosomal RNA. HAMAP MF_00360

Subunit structure

Part of the 30S ribosomal subunit. Interacts weakly with L2 in one of the 3.5 A resolved structures (Ref.14). HAMAP MF_00360

Post-translational modification

5 different forms of the protein, varying only in the number of C-terminal glutamate residues, were isolated. The sequence shown is form S6-6, which is the longest. The first two Glu are encoded by the rpsF gene, the other Glu are added post-translationally by the rimK enzyme. HAMAP MF_00360

Sequence similarities

Belongs to the ribosomal protein S6P family.

Mass spectrometry

Molecular mass is 15187.2 Da from positions 1 - 131. Determined by MALDI. Ref.10

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13513530S ribosomal protein S6, fully modified isoform HAMAP MF_00360
PRO_0000030640
Chain1 – 13113130S ribosomal protein S6, non-modified isoform HAMAP MF_00360
PRO_0000030641

Amino acid modifications

Modified residue931N6-acetyllysine Ref.11

Experimental info

Sequence conflict141Q → T AA sequence Ref.6
Sequence conflict201G → A AA sequence Ref.6

Secondary structure

.............. 135
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P02358-1 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: F4CC629711C1FD0E

FASTA13515,703
        10         20         30         40         50         60 
MRHYEIVFMV HPDQSEQVPG MIERYTAAIT GAEGKIHRLE DWGRRQLAYP INKLHKAHYV 

        70         80         90        100        110        120 
LMNVEAPQEV IDELETTFRF NDAVIRSMVM RTKHAVTEAS PMVKAKDERR ERRDDFANET 

       130 
ADDAEAGDSE EEEEE 

« Hide

References

« Hide 'large scale' references
[1]"Determination of the complete amino-acid sequence of protein S6 from the wild-type and a mutant of Escherichia coli."
Hitz H., Schaefer D., Wittmann-Liebold B.
Eur. J. Biochem. 75:497-512(1977) [PubMed: 328274] [Abstract]
Cited for: PROTEIN SEQUENCE.
Strain: K.
[2]"The nucleotide sequence of an Escherichia coli chromosomal region containing the genes for ribosomal proteins S6, S18, L9 and an open reading frame."
Schnier J., Kitakawa M., Isono K.
Mol. Gen. Genet. 204:126-132(1986) [PubMed: 3528756] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-131.
[3]"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 1-131.
Strain: K12 / MG1655 / ATCC 47076.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 1-131.
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 1-131.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"Small genes/gene-products in Escherichia coli K-12."
Wasinger V.C., Humphery-Smith I.
FEMS Microbiol. Lett. 169:375-382(1998) [PubMed: 9868784] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-20.
Strain: K12.
[7]Pasquali C., Sanchez J.-C., Ravier F., Golaz O., Hughes G.J., Frutiger S., Paquet N., Wilkins M., Appel R.D., Bairoch A., Hochstrasser D.F.
Submitted (SEP-1994) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 1-11.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[8]"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
Link A.J., Robison K., Church G.M.
Electrophoresis 18:1259-1313(1997) [PubMed: 9298646] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-12.
Strain: K12 / EMG2.
[9]"Protein identification with N and C-terminal sequence tags in proteome projects."
Wilkins M.R., Gasteiger E., Tonella L., Ou K., Tyler M., Sanchez J.-C., Gooley A.A., Walsh B.J., Bairoch A., Appel R.D., Williams K.L., Hochstrasser D.F.
J. Mol. Biol. 278:599-608(1998) [PubMed: 9600841] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-4.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[10]"Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry."
Arnold R.J., Reilly J.P.
Anal. Biochem. 269:105-112(1999) [PubMed: 10094780] [Abstract]
Cited for: MASS SPECTROMETRY.
Strain: K12 / ATCC 25404 / DSM 5698 / NCIMB 11290.
[11]"Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli."
Zhang J., Sprung R., Pei J., Tan X., Kim S., Zhu H., Liu C.F., Grishin N.V., Zhao Y.
Mol. Cell. Proteomics 8:215-225(2009) [PubMed: 18723842] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-93, MASS SPECTROMETRY.
[12]"All-atom homology model of the Escherichia coli 30S ribosomal subunit."
Tung C.-S., Joseph S., Sanbonmatsu K.Y.
Nat. Struct. Biol. 9:750-755(2002) [PubMed: 12244297] [Abstract]
Cited for: 3D-STRUCTURE MODELING.
[13]"Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement."
Gao H., Sengupta J., Valle M., Korostelev A., Eswar N., Stagg S.M., Van Roey P., Agrawal R.K., Harvey S.C., Sali A., Chapman M.S., Frank J.
Cell 113:789-801(2003) [PubMed: 12809609] [Abstract]
Cited for: STRUCTURE BY ELECTRON MICROSCOPY (11.50 ANGSTROMS).
Strain: MRE-600.
[14]"Structures of the bacterial ribosome at 3.5 A resolution."
Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W., Vila-Sanjurjo A., Holton J.M., Cate J.H.D.
Science 310:827-834(2005) [PubMed: 16272117] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.46 ANGSTROMS) OF 2 DIFFERENT RIBOSOME STRUCTURES.
Strain: MRE-600.
+Additional computationally mapped references.

Cross-references

Sequence databases

X04022 Genomic DNA. Translation: CAA27652.1.
U14003 Genomic DNA. Translation: AAA97096.1.
U00096 Genomic DNA. Translation: AAC77157.2.
AP009048 Genomic DNA. Translation: BAE78201.1.
PIRR3EC6. C65231.
RefSeqAP_004700.1.
NP_418621.2.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1M5Gmodel-F1-100[»]
1P6Gelectron microscopy12.30F1-135[»]
1P87electron microscopy11.50F1-135[»]
1VS5X-ray3.46F1-135[»]
1VS7X-ray3.46F1-135[»]
2AVYX-ray3.46F1-135[»]
2AW7X-ray3.46F1-135[»]
2GY9electron microscopy2.00F1-95[»]
2GYBelectron microscopy2.00F1-95[»]
2I2PX-ray3.22F1-135[»]
2I2UX-ray3.22F1-135[»]
2QALX-ray3.21F1-135[»]
2QANX-ray3.21F1-135[»]
2QB9X-ray3.54F1-135[»]
2QBBX-ray3.54F1-135[»]
2QBDX-ray3.30F1-135[»]
2QBFX-ray3.30F1-135[»]
2QBHX-ray4.00F1-135[»]
2QBJX-ray4.00F1-135[»]
2QOUX-ray3.93F1-135[»]
2QOWX-ray3.93F1-135[»]
2QOYX-ray3.50F1-135[»]
2QP0X-ray3.50F1-135[»]
2VHOX-ray3.74F1-135[»]
2VHPX-ray3.74F1-135[»]
2Z4KX-ray4.45F1-135[»]
2Z4MX-ray4.45F1-135[»]
3DF1X-ray3.50F1-135[»]
3DF3X-ray3.50F1-135[»]
3FIHelectron microscopy-F1-100[»]
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:10782N.

2-D gel databases

SWISS-2DPAGEP02358.
ECO2DBASEC014.8. 6TH EDITION.
C015.3. 6TH EDITION.
D014.7. 6TH EDITION.

Genome annotation databases

GeneID948723.
GenomeReviewsGene locus JW4158 in contig AP009048_GR.
Gene locus b4200 in contig U00096_GR.
KEGGecj:JW4158.
eco:b4200.

Organism-specific databases

EchoBASEEB0898.
EcoGeneEG10905. rpsF.
CMRSearch...

Phylogenomic databases

HOGENOMP02358.
OMAP02358. MSFYESV.

Enzyme and pathway databases

BioCycEcoCyc:EG10905-MON.

Family and domain databases

HAMAPMF_00360.
[Tree]
InterProIPR000529. Ribosomal_S6.
IPR014717. Transl_elong_EF1B/ribosomal_S6.
[Graphical view]
Gene3DG3DSA:3.30.70.60. Transl_elong_EF1B/rib_con. 1 hit.
PfamPF01250. Ribosomal_S6. 1 hit.
[Graphical view]
ProDomPD003809. Ribosomal_S6. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR00166. S6. 1 hit.
PROSITEPS01048. RIBOSOMAL_S6. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRS6_ECOLI
AccessionPrimary (citable) accession number: P02358
Secondary accession number(s): Q2M6A5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: June 16, 2009
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Ribosomal proteins

Ribosomal proteins families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents