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Protein

Histone H4

Gene

HHF1

more
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Component of the UAF (upstream activation factor) complex which interacts with the upstream element of the RNA polymerase I promoter and forms a stable preinitiation complex. Together with SPT15/TBP UAF seems to stimulate basal transcription to a fully activated level.

Miscellaneous

Present with 524000 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi17 – 215

GO - Molecular functioni

  • DNA binding Source: SGD
  • histone binding Source: GO_Central
  • protein heterodimerization activity Source: InterPro

GO - Biological processi

  • chromatin assembly or disassembly Source: SGD
  • histone H3-K79 methylation Source: SGD
  • nucleosome assembly Source: GO_Central
  • sexual sporulation resulting in formation of a cellular spore Source: SGD
  • transfer RNA gene-mediated silencing Source: SGD

Keywordsi

Molecular functionDNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-28996-MONOMER.
YEAST:G3O-33067-MONOMER.
ReactomeiR-SCE-2299718. Condensation of Prophase Chromosomes.
R-SCE-2559580. Oxidative Stress Induced Senescence.
R-SCE-2559582. Senescence-Associated Secretory Phenotype (SASP).
R-SCE-3214815. HDACs deacetylate histones.
R-SCE-3214842. HDMs demethylate histones.
R-SCE-3214847. HATs acetylate histones.
R-SCE-3214858. RMTs methylate histone arginines.
R-SCE-427359. SIRT1 negatively regulates rRNA Expression.
R-SCE-4551638. SUMOylation of chromatin organization proteins.
R-SCE-5625886. Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3.
R-SCE-5693565. Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks.
R-SCE-8936459. RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function.

Names & Taxonomyi

Protein namesi
Recommended name:
Histone H4
Gene namesi
Name:HHF1
Ordered Locus Names:YBR009C
ORF Names:YBR0122
AND
Name:HHF2
Ordered Locus Names:YNL030W
ORF Names:N2752
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componentsi: Chromosome II, Chromosome XIV

Organism-specific databases

SGDiS000000213. HHF1.
S000004975. HHF2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleosome core, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCurated
ChainiPRO_00001583772 – 103Histone H4Add BLAST102

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei6N6-acetyllysine1 Publication1
Modified residuei13N6-acetyllysine1 Publication1
Modified residuei17N6-acetyllysine3 Publications1
Modified residuei61PhosphoserineCombined sources1
Modified residuei65PhosphoserineCombined sources1
Modified residuei80N6-acetyllysine1 Publication1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP02309.
PRIDEiP02309.

PTM databases

iPTMnetiP02309.

Interactioni

Subunit structurei

The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Histone H4 is a component of the UAF (upstream activation factor) complex which consists of UAF30, RRN5, RRN9, RRN10, and histones H3 and H4.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi32710. 695 interactors.
35797. 474 interactors.
DIPiDIP-418N.
IntActiP02309. 337 interactors.
MINTiMINT-613388.
STRINGi4932.YNL030W.

Structurei

Secondary structure

1103
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 7Combined sources4
Beta strandi16 – 19Combined sources4
Helixi26 – 29Combined sources4
Helixi32 – 40Combined sources9
Turni41 – 43Combined sources3
Turni46 – 49Combined sources4
Helixi51 – 76Combined sources26
Beta strandi80 – 82Combined sources3
Helixi84 – 93Combined sources10
Beta strandi97 – 101Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E6IX-ray1.87P16-30[»]
1ID3X-ray3.10B/F2-103[»]
1Q1AX-ray1.50B13-22[»]
1SZCX-ray1.75B13-22[»]
1SZDX-ray1.50B13-22[»]
2DVQX-ray2.04P/Q2-16[»]
2DVRX-ray2.30P/Q2-16[»]
2E3KX-ray2.30Q/R2-16[»]
2FSBmodel-B/F1-103[»]
2H2HX-ray2.20B76-86[»]
2L5ANMR-A42-103[»]
2QQFX-ray2.00B13-23[»]
2QQGX-ray2.05B13-23[»]
3TO6X-ray2.10B12-23[»]
4JJNX-ray3.09B/F2-103[»]
4KUDX-ray3.20B/F1-103[»]
4PSXX-ray2.51C/F2-49[»]
4TWIX-ray1.79B9-21[»]
4TWJX-ray1.65B9-21[»]
ProteinModelPortaliP02309.
SMRiP02309.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP02309.

Family & Domainsi

Sequence similaritiesi

Belongs to the histone H4 family.Curated

Phylogenomic databases

GeneTreeiENSGT00760000119019.
HOGENOMiHOG000234654.
InParanoidiP02309.
KOiK11254.
OMAiYEEVRVV.
OrthoDBiEOG092C5WME.

Family and domain databases

CDDicd00076. H4. 1 hit.
InterProiView protein in InterPro
IPR035425. CENP-T/H4_C.
IPR009072. Histone-fold.
IPR001951. Histone_H4.
IPR019809. Histone_H4_CS.
PfamiView protein in Pfam
PF15511. CENP-T_C. 1 hit.
PRINTSiPR00623. HISTONEH4.
SMARTiView protein in SMART
SM00417. H4. 1 hit.
SUPFAMiSSF47113. SSF47113. 1 hit.
PROSITEiView protein in PROSITE
PS00047. HISTONE_H4. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02309-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGRGKGGKG LGKGGAKRHR KILRDNIQGI TKPAIRRLAR RGGVKRISGL
60 70 80 90 100
IYEEVRAVLK SFLESVIRDS VTYTEHAKRK TVTSLDVVYA LKRQGRTLYG

FGG
Length:103
Mass (Da):11,368
Last modified:January 23, 2007 - v2
Checksum:i69B7D1F89E62DE41
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti46R → K AA sequence (PubMed:7002547).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00724 Genomic DNA. Translation: CAA25311.1.
X00725 Genomic DNA. Translation: CAA25313.1.
K03154 Genomic DNA. Translation: AAA34660.1.
Z35878 Genomic DNA. Translation: CAA84947.1.
Z71306 Genomic DNA. Translation: CAA95892.1.
AY692960 Genomic DNA. Translation: AAT92979.1.
BK006936 Genomic DNA. Translation: DAA07130.1.
BK006947 Genomic DNA. Translation: DAA10515.1.
PIRiA02647. HSBY4.
RefSeqiNP_009563.1. NM_001178357.1.
NP_014368.1. NM_001182869.1.

Genome annotation databases

EnsemblFungiiYBR009C; YBR009C; YBR009C.
YNL030W; YNL030W; YNL030W.
GeneIDi852294.
855701.
KEGGisce:YBR009C.
sce:YNL030W.

Similar proteinsi

Entry informationi

Entry nameiH4_YEAST
AccessioniPrimary (citable) accession number: P02309
Secondary accession number(s): D6VQ10
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: September 27, 2017
This is version 188 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names
  5. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names