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Protein

Histone H2B

Gene

His2B

more
Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Caution

Was reported to be phosphorylated at Ser-34 (PubMed:15143281). However, the paper was retracted because some data, results and conclusions in the paper are not reliable.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding

Enzyme and pathway databases

ReactomeiR-DME-201722 Formation of the beta-catenin:TCF transactivating complex
R-DME-212300 PRC2 methylates histones and DNA
R-DME-2299718 Condensation of Prophase Chromosomes
R-DME-2559580 Oxidative Stress Induced Senescence
R-DME-2559582 Senescence-Associated Secretory Phenotype (SASP)
R-DME-3214815 HDACs deacetylate histones
R-DME-3214847 HATs acetylate histones
R-DME-427359 SIRT1 negatively regulates rRNA expression
R-DME-427413 NoRC negatively regulates rRNA expression
R-DME-5578749 Transcriptional regulation by small RNAs
R-DME-5625886 Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3
R-DME-5689880 Ub-specific processing proteases
R-DME-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks
R-DME-5693571 Nonhomologous End-Joining (NHEJ)
R-DME-73777 RNA Polymerase I Chain Elongation
R-DME-8866654 E3 ubiquitin ligases ubiquitinate target proteins
R-DME-8936459 RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function
R-DME-9018519 Estrogen-dependent gene expression

Names & Taxonomyi

Protein namesi
Recommended name:
Histone H2B
Gene namesi
Name:His2B
AND
ORF Names:CG17949
AND
ORF Names:CG33868
AND
ORF Names:CG33870
AND
ORF Names:CG33872
AND
ORF Names:CG33874
AND
ORF Names:CG33876
AND
ORF Names:CG33878
AND
ORF Names:CG33880
AND
ORF Names:CG33882
AND
ORF Names:CG33884
AND
ORF Names:CG33886
AND
ORF Names:CG33888
AND
ORF Names:CG33890
AND
ORF Names:CG33892
AND
ORF Names:CG33894
AND
ORF Names:CG33896
AND
ORF Names:CG33898
AND
ORF Names:CG33900
AND
ORF Names:CG33902
AND
ORF Names:CG33904
AND
ORF Names:CG33906
AND
ORF Names:CG33908
AND
ORF Names:CG33910
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0001198 His2B
FBgn0061209 His2B:CG17949
FBgn0053868 His2B:CG33868
FBgn0053870 His2B:CG33870
FBgn0053872 His2B:CG33872
FBgn0053874 His2B:CG33874
FBgn0053876 His2B:CG33876
FBgn0053878 His2B:CG33878
FBgn0053880 His2B:CG33880
FBgn0053882 His2B:CG33882
FBgn0053884 His2B:CG33884
FBgn0053886 His2B:CG33886
FBgn0053888 His2B:CG33888
FBgn0053890 His2B:CG33890
FBgn0053892 His2B:CG33892
FBgn0053894 His2B:CG33894
FBgn0053896 His2B:CG33896
FBgn0053898 His2B:CG33898
FBgn0053900 His2B:CG33900
FBgn0053902 His2B:CG33902
FBgn0053904 His2B:CG33904
FBgn0053906 His2B:CG33906
FBgn0053908 His2B:CG33908
FBgn0053910 His2B:CG33910

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleosome core, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00000718612 – 123Histone H2BAdd BLAST122

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-methylproline; partial1 Publication1
Modified residuei44N6-succinyllysine1 Publication1
Glycosylationi110O-linked (GlcNAc) serineBy similarity1
Modified residuei114N6-succinyllysine1 Publication1
Modified residuei118N6-succinyllysine1 Publication1
Cross-linki118Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Curated

Post-translational modificationi

Monoubiquitination of Lys-118 by Bre1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation.1 Publication
Methylation at Pro-2 increases upon heat shock.1 Publication
GlcNAcylation at Ser-110 promotes monoubiquitination of Lys-118. It fluctuates in response to extracellular glucose, and associates with transcribed genes (Probable).Curated

Keywords - PTMi

Glycoprotein, Isopeptide bond, Methylation, Ubl conjugation

Proteomic databases

PaxDbiP02283
PRIDEiP02283

PTM databases

iPTMnetiP02283

Expressioni

Gene expression databases

BgeeiFBgn0053868
GenevisibleiP02283 DM

Interactioni

Subunit structurei

The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.

Binary interactionsi

WithEntry#Exp.IntActNotes
ATXN7O152652EBI-188137,EBI-708350From Homo sapiens.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi533862, 1 interactor
77520, 19 interactors
DIPiDIP-22804N
IntActiP02283, 8 interactors
MINTiP02283
STRINGi7227.FBpp0091155

Structurei

Secondary structure

1123
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi36 – 46Combined sources11
Helixi54 – 81Combined sources28
Helixi89 – 99Combined sources11
Helixi102 – 121Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2NQBX-ray2.30D/H2-123[»]
2PYOX-ray2.43D/H2-123[»]
4QLCX-ray3.50D/H2-123[»]
4X23X-ray3.50D/H/N/R33-122[»]
5CVEX-ray1.50D/E3-10[»]
ProteinModelPortaliP02283
SMRiP02283
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP02283

Family & Domainsi

Sequence similaritiesi

Belongs to the histone H2B family.Curated

Phylogenomic databases

eggNOGiKOG1744 Eukaryota
ENOG4111NV5 LUCA
GeneTreeiENSGT00760000118976
InParanoidiP02283
KOiK11252
OMAiSKEMSIM
OrthoDBiEOG091G0XGD
PhylomeDBiP02283

Family and domain databases

Gene3Di1.10.20.10, 1 hit
InterProiView protein in InterPro
IPR009072 Histone-fold
IPR007125 Histone_H2A/H2B/H3
IPR000558 Histone_H2B
PANTHERiPTHR23428 PTHR23428, 1 hit
PfamiView protein in Pfam
PF00125 Histone, 1 hit
PRINTSiPR00621 HISTONEH2B
SMARTiView protein in SMART
SM00427 H2B, 1 hit
SUPFAMiSSF47113 SSF47113, 1 hit
PROSITEiView protein in PROSITE
PS00357 HISTONE_H2B, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02283-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPKTSGKAA KKAGKAQKNI TKTDKKKKRK RKESYAIYIY KVLKQVHPDT
60 70 80 90 100
GISSKAMSIM NSFVNDIFER IAAEASRLAH YNKRSTITSR EIQTAVRLLL
110 120
PGELAKHAVS EGTKAVTKYT SSK
Length:123
Mass (Da):13,696
Last modified:January 23, 2007 - v2
Checksum:i0774D25F34003062
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti77R → C AA sequence (PubMed:117830).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14215 Genomic DNA Translation: CAA32432.1
AE014134 Genomic DNA Translation: AAN11124.1
AE014134 Genomic DNA Translation: AAZ66483.1
AE014134 Genomic DNA Translation: AAZ66487.1
AE014134 Genomic DNA Translation: AAZ66492.1
AE014134 Genomic DNA Translation: AAZ66496.1
AE014134 Genomic DNA Translation: AAZ66501.1
AE014134 Genomic DNA Translation: AAZ66506.1
AE014134 Genomic DNA Translation: AAZ66511.1
AE014134 Genomic DNA Translation: AAZ66521.1
AE014134 Genomic DNA Translation: AAZ66531.1
AE014134 Genomic DNA Translation: AAZ66576.1
AE014134 Genomic DNA Translation: AAZ66571.1
AE014134 Genomic DNA Translation: AAZ66566.1
AE014134 Genomic DNA Translation: AAZ66561.1
AE014134 Genomic DNA Translation: AAZ66556.1
AE014134 Genomic DNA Translation: AAZ66551.1
AE014134 Genomic DNA Translation: AAZ66546.1
AE014134 Genomic DNA Translation: AAZ66541.1
AE014134 Genomic DNA Translation: AAZ66536.1
AE014134 Genomic DNA Translation: AAZ66479.1
AE014134 Genomic DNA Translation: AAZ66581.1
AE014134 Genomic DNA Translation: AAZ66526.1
AE014134 Genomic DNA Translation: AAZ66516.1
PIRiS10095 HSFF22
RefSeqiNP_001027283.1, NM_001032112.2
NP_001027287.1, NM_001032116.2
NP_001027291.1, NM_001032120.2
NP_001027296.1, NM_001032125.2
NP_001027300.1, NM_001032129.2
NP_001027305.1, NM_001032134.2
NP_001027310.1, NM_001032139.2
NP_001027315.1, NM_001032144.2
NP_001027320.1, NM_001032149.2
NP_001027325.1, NM_001032154.2
NP_001027330.1, NM_001032159.2
NP_001027335.1, NM_001032164.2
NP_001027340.1, NM_001032169.2
NP_001027345.1, NM_001032174.2
NP_001027350.1, NM_001032179.2
NP_001027355.1, NM_001032184.2
NP_001027360.1, NM_001032189.2
NP_001027365.1, NM_001032194.2
NP_001027370.1, NM_001032199.2
NP_001027375.1, NM_001032204.2
NP_001027380.1, NM_001032209.2
NP_001027385.1, NM_001032214.2
NP_724342.1, NM_165381.4

Genome annotation databases

EnsemblMetazoaiFBtr0085927; FBpp0085281; FBgn0061209
FBtr0091872; FBpp0091113; FBgn0053868
FBtr0091874; FBpp0091115; FBgn0053870
FBtr0091876; FBpp0091117; FBgn0053872
FBtr0091878; FBpp0091119; FBgn0053874
FBtr0091880; FBpp0091121; FBgn0053876
FBtr0091882; FBpp0091123; FBgn0053878
FBtr0091884; FBpp0091125; FBgn0053880
FBtr0091886; FBpp0091127; FBgn0053882
FBtr0091888; FBpp0091129; FBgn0053884
FBtr0091890; FBpp0091131; FBgn0053886
FBtr0091892; FBpp0091133; FBgn0053888
FBtr0091894; FBpp0091135; FBgn0053890
FBtr0091896; FBpp0091137; FBgn0053892
FBtr0091898; FBpp0091139; FBgn0053894
FBtr0091900; FBpp0091141; FBgn0053896
FBtr0091902; FBpp0091143; FBgn0053898
FBtr0091904; FBpp0091145; FBgn0053900
FBtr0091906; FBpp0091147; FBgn0053902
FBtr0091908; FBpp0091149; FBgn0053904
FBtr0091910; FBpp0091151; FBgn0053906
FBtr0091912; FBpp0091153; FBgn0053908
FBtr0091914; FBpp0091155; FBgn0053910
GeneIDi326273
3771809
3771891
3771957
3772013
3772058
3772081
3772083
3772094
3772099
3772104
3772166
3772203
3772248
3772264
3772265
3772271
3772276
3772299
3772336
3772496
3772502
3772575
KEGGidme:Dmel_CG17949
dme:Dmel_CG33868
dme:Dmel_CG33870
dme:Dmel_CG33872
dme:Dmel_CG33874
dme:Dmel_CG33876
dme:Dmel_CG33878
dme:Dmel_CG33880
dme:Dmel_CG33882
dme:Dmel_CG33884
dme:Dmel_CG33886
dme:Dmel_CG33888
dme:Dmel_CG33890
dme:Dmel_CG33892
dme:Dmel_CG33894
dme:Dmel_CG33896
dme:Dmel_CG33898
dme:Dmel_CG33900
dme:Dmel_CG33902
dme:Dmel_CG33904
dme:Dmel_CG33906
dme:Dmel_CG33908
dme:Dmel_CG33910
UCSCiCG17949-RA d. melanogaster

Similar proteinsi

Entry informationi

Entry nameiH2B_DROME
AccessioniPrimary (citable) accession number: P02283
Secondary accession number(s): Q4ABE1, Q9W5U7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 184 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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