P02239 (LGB1_LUPLU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Leghemoglobin-1 Alternative name(s): Leghemoglobin I
Organism	Lupinus luteus (European yellow lupin)
Taxonomic identifier	3873 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Genisteae › Lupinus

Protein attributes

Sequence length	154 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Provides oxygen to the bacteroids. This role is essential for symbiotic nitrogen fixation.
Subunit structure	Monomer.
Tissue specificity	Root nodules.
Sequence similarities	Belongs to the plant globin family.

Ontologies

Keywords
Biological process	Nitrogen fixation Oxygen transport Transport
Ligand	Heme Iron Metal-binding
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	nitrogen fixation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro oxygen binding Inferred from electronic annotation. Source: InterPro oxygen transporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.4
	Chain	2 – 154	153	Leghemoglobin-1		PRO_0000192984
Sites
	Metal binding	64	1	Iron (heme distal ligand) By similarity
	Metal binding	98	1	Iron (heme proximal ligand) By similarity
Experimental info
	Sequence conflict	80	1	Q → E AA sequence Ref.4
	Sequence conflict	121	1	E → G in CAA68462. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P02239 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 58101C830CB21F14	FASTA	154	16,753
	10 20 30 40 50 60 MGVLTDVQVA LVKSSFEEFN ANIPKNTHRF FTLVLEIAPG AKDLFSFLKG SSEVPQNNPD 70 80 90 100 110 120 LQAHAGKVFK LTYEAAIQLQ VNGAVASDAT LKSLGSVHVS KGVVDAHFPV VKEAILKTIK 130 140 150 EVVGDKWSEE LNTAWTIAYD ELAIIIKKEM KDAA

References

[1]	"Nucleotide sequence of lupin leghemoglobin I cDNA." Konieczny A. Nucleic Acids Res. 15:6742-6742(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Strozycki P.S.P. Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: cv. Ventus. Tissue: Root nodule.
[3]	"Yellow lupine gene coding for leghemoglobin I." Strozycki P.M., Karlowski W.M., Legocki A.B. Plant Gene Register PGR98-017 Cited for: NUCLEOTIDE SEQUENCE. Strain: cv. Ventus.
[4]	"The complete amino acid sequence of the leghemoglobin I from yellow lupin root nodules." Egorov T.A., Feigina M.Y., Kazakov V.K., Shakhparonov M.I., Mimaleva S.I., Ovchinnikov Y.A. Bioorg. Khim. 2:125-128(1976) Cited for: PROTEIN SEQUENCE OF 2-154. Tissue: Root nodule.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y00401 mRNA. Translation: CAA68462.1. X77043 mRNA. Translation: CAA54332.1. U50083 Genomic DNA. Translation: AAC04853.1.
PIR	GPYL. A26808.
3D structure databases
ProteinModelPortal	P02239.
SMR	P02239. Positions 2-154.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.10.490.10. 1 hit.
InterPro	IPR000971. Globin. IPR009050. Globin-like. IPR012292. Globin_dom. IPR001032. Leghaemoglobin. IPR019824. Leghaemoglobin_Fe_BS. [Graphical view]
Pfam	PF00042. Globin. 1 hit. [Graphical view]
PRINTS	PR00188. PLANTGLOBIN.
SUPFAM	SSF46458. Globin_like. 1 hit.
PROSITE	PS01033. GLOBIN. 1 hit. PS00208. PLANT_GLOBIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LGB1_LUPLU

Accession

Primary (citable) accession number: P02239

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 81 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program