P02232 (LGB1_VICFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Leghemoglobin-1 Alternative name(s): Leghemoglobin I
Organism	Vicia faba (Broad bean) (Faba vulgaris)
Taxonomic identifier	3906 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Fabeae › Vicia

Protein attributes

Sequence length	144 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Provides oxygen to the bacteroids. This role is essential for symbiotic nitrogen fixation.
Subunit structure	Monomer.
Tissue specificity	Root nodules.
Miscellaneous	Asp-5, Gln-6, Gly-23, Gly-24, Lys-35, Gly-42, Gln-57, Gln-65, Glu-72, Ile-75, Gln-78, Glu-127, Ile-129, and Glu-135 were also found in this pooled preparation.
Sequence similarities	Belongs to the plant globin family.

Ontologies

Keywords
Biological process	Nitrogen fixation Oxygen transport Transport
Ligand	Heme Iron Metal-binding
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	nitrogen fixation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro oxygen binding Inferred from electronic annotation. Source: InterPro oxygen transporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 144	143	Leghemoglobin-1		PRO_0000193006
Sites
	Metal binding	62	1	Iron (heme distal ligand) By similarity
	Metal binding	93	1	Iron (heme proximal ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P02232 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 6D26E91BD3AD6F22	FASTA	144	15,531
	10 20 30 40 50 60 MGFTEKQEAL VNSSSQLFKQ NPSNYSVLFY TIILQKAPTA KAMFSFLKDS AGVVDSPKLG 70 80 90 100 110 120 AHAEKVFGMV RDSAVQLRAT GEVVLDGKDG SIHIQKGVLD PHFVVVKEAL LKTIKEASGD 130 140 KWSEELSAAW EVAYDGLATA IKAA

References

[1]	"The amino acid sequence of leghaemoglobin I from root nodules of broad bean (Vicia faba L.)." Richardson M., Dilworth M.J., Scawen M.D. FEBS Lett. 51:33-37(1975) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-144. Tissue: Root nodule.

Cross-references

Sequence databases
PIR	GPVF. A02554.
3D structure databases
ProteinModelPortal	P02232.
SMR	P02232. Positions 1-144.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.10.490.10. 1 hit.
InterPro	IPR000971. Globin. IPR009050. Globin-like. IPR012292. Globin_dom. IPR001032. Leghaemoglobin. IPR019824. Leghaemoglobin_Fe_BS. [Graphical view]
Pfam	PF00042. Globin. 1 hit. [Graphical view]
PRINTS	PR00188. PLANTGLOBIN.
SUPFAM	SSF46458. Globin_like. 1 hit.
PROSITE	PS01033. GLOBIN. 1 hit. PS00208. PLANT_GLOBIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LGB1_VICFA

Accession

Primary (citable) accession number: P02232

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program