P02221 (GLB1_CHITH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Globin CTT-I/CTT-IA

Alternative name(s):
Erythrocruorin

Gene names

Name:

CTT-1

Organism

Chironomus thummi thummi (Midge)

Taxonomic identifier

7155 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Nematocera › Culicimorpha › Chironomoidea › Chironomidae › Chironominae › Chironomini › Chironomus › Chironomus › Chironomus thummi

Protein attributes

Sequence length	158 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Monomer.
Miscellaneous	There are at least 12 different components in Midge globin.
Sequence similarities	Belongs to the globin family.

Ontologies

Keywords
Biological process	Oxygen transport Transport
Domain	Signal
Ligand	Heme Iron Metal-binding
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular_component	extracellular region Inferred from electronic annotation. Source: InterPro hemoglobin complex Inferred from electronic annotation. Source: InterPro
Molecular_function	heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro oxygen binding Inferred from electronic annotation. Source: InterPro oxygen transporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15	Ref.2 Ref.3
	Chain	16 – 158	143	Globin CTT-I/CTT-IA		PRO_0000011188
Sites
	Metal binding	74	1	Iron (heme distal ligand) By similarity
	Metal binding	109	1	Iron (heme proximal ligand) By similarity
Natural variations
	Natural variant	113	1	A → T in CTT-IA.

Sequences

	Sequence		Length	Mass (Da)
	P02221 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 9639E8A38908B8AB	FASTA	158	17,012
	10 20 30 40 50 60 MKFLILALCV AAAMAGPSGD QIAAAKASWN TVKNNQVDIL YAVFKANPDI QTAFSQFAGK 70 80 90 100 110 120 DLDSIKGTPD FSKHAGRVVG LFSEVMDLLG NDANTPTILA KAKDFGKSHK SRASPAQLDN 130 140 150 FRKSLVVYLK GATKWDSAVE SSWAPVLDFV FSTLKNEL

References

[1]	"Sequence and evolution of the gene for the monomeric globin I and its linkage to genes coding for dimeric globins in the insect Chironomus thummi." Kao W.-Y., Hankeln T., Schmidt E.R., Bergtrom G. J. Mol. Evol. 40:354-361(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (CTT-I).
[2]	"Hemoglobin, XXXI. Analysis or the primary structure of the monomeric hemoglobin CTT I (erythrocruorin) of Chironomus thummi thummi, Diptera." Kleinschmidt T., von der Mark-Neuwirth H., Braunitzer G. Hoppe-Seyler's Z. Physiol. Chem. 361:401-411(1980) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 16-158 (CTT-I).
[3]	"The analysis of a protein-polymorphism. Evolution of monomeric and homodimeric haemoglobins (erythrocruorins) of Chironomus thummi thummi (Insecta, Diptera)." Goodman M., Braunitzer G., Kleinschmidt T., Aschauer H. Hoppe-Seyler's Z. Physiol. Chem. 364:205-217(1983) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 16-158 (CTT-IA).
[4]	"Differential regulation of insect globin and actin mRNAs during larval development in Chironomus thummi." Saffarini D.A., Trewitt P.M., Luhm R.A., Bergtrom G. Gene 101:215-222(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 83-158 (CTT-IA).

Cross-references

Sequence databases
EMBL GenBank DDBJ	U14627 Genomic DNA. Translation: AAA80189.1. U14628 Genomic DNA. Translation: AAA80190.1. U14629 Genomic DNA. Translation: AAA80191.1. M57410 mRNA. Translation: AAA62727.1.
PIR	GGICE1. A91690. GGIC1A. A91719.
3D structure databases
ProteinModelPortal	P02221.
ModBase	Search...
Protein family/group databases
Allergome	1650. Chi t 2. 205. Chi t 2.0101. 206. Chi t 2.0102.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.10.490.10. 1 hit.
InterPro	IPR002336. Erythrocruorin. IPR000971. Globin. IPR009050. Globin-like. IPR012292. Globin_dom. [Graphical view]
Pfam	PF00042. Globin. 1 hit. [Graphical view]
PRINTS	PR00611. ERYTHCRUORIN.
SUPFAM	SSF46458. Globin_like. 1 hit.
PROSITE	PS01033. GLOBIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLB1_CHITH

Accession

Primary (citable) accession number: P02221

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	November 1, 1997
Last modified:	April 3, 2013
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)