P02208 (GLB5_PETMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Globin-5 Alternative name(s): Hemoglobin V
Organism	Petromyzon marinus (Sea lamprey)
Taxonomic identifier	7757 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Hyperoartia › Petromyzontiformes › Petromyzontidae › Petromyzon

Protein attributes

Sequence length	150 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Monomer at high oxygen tension and high pH and dimeric at low oxygen tension and lower pH.
Miscellaneous	Globin V is the major component of the 6 globins found in the Sea lamprey.
Sequence similarities	Belongs to the globin family.

Ontologies

Keywords
Biological process	Oxygen transport Transport
Ligand	Heme Iron Metal-binding
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Molecular_function	heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro oxygen binding Inferred from electronic annotation. Source: InterPro oxygen transporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed By similarity

Chain

2 – 150

149

Globin-5

PRO_0000052529

Sites

Metal binding

74

1

Iron (heme distal ligand) Ref.4

Metal binding

106

1

Iron (heme proximal ligand) Ref.4

Natural variations

Natural variant

30

1

T → N.

Experimental info

Sequence conflict

96

1

S → SS Ref.3

Sequence conflict

99 – 100

2

Missing AA sequence Ref.2

Sequence conflict

99 – 100

2

Missing Ref.3

Sequence conflict

101

1

D → N AA sequence Ref.2

Sequence conflict

139

1

M → R AA sequence Ref.2

Sequence conflict

139

1

M → R Ref.3

Sequence conflict

140

1

Missing AA sequence Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

150

Helix Strand Turn

Details...

Helix

14 – 29

16

Helix

31 – 45

15

Helix

47 – 52

6

Helix

54 – 56

3

Helix

62 – 67

6

Helix

69 – 87

19

Turn

88 – 90

3

Helix

92 – 108

17

Helix

114 – 116

3

Helix

117 – 129

13

Helix

133 – 147

15

Sequences

	Sequence		Length	Mass (Da)
	P02208 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 39BE05B24670A88A	FASTA	150	16,401
	10 20 30 40 50 60 MPIVDTGSVA PLSAAEKTKI RSAWAPVYST YETSGVDILV KFFTSTPAAQ EFFPKFKGLT 70 80 90 100 110 120 TADQLKKSAD VRWHAERIIN AVNDAVASMD DTEKMSMKLR DLSGKHAKSF QVDPQYFKVL 130 140 150 AAVIADTVAA GDAGFEKLMS MICILLRSAY

References

[1]	"Haemoglobins, LX. Primary structure of the major haemoglobin of the sea lamprey Petromyzon marinus (var. Garonne, Loire)." Hombrados I., Rodewald K., Neuzil E., Braunitzer G. Biochimie 65:247-257(1983) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-150.
[2]	"The amino acid sequence of hemoglobin V from the lamprey, Petromyzon marinus." Li S.L., Riggs A. J. Biol. Chem. 245:6149-6169(1970) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-150.
[3]	"Crystal structure analysis of sea lamprey hemoglobin at 2-A resolution." Hendrickson W.A., Love W.E., Karle J. J. Mol. Biol. 74:331-361(1973) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
[4]	"Refinement of a molecular model for lamprey hemoglobin from Petromyzon marinus." Honzatko R.B., Hendrickson W.A., Love W.E. J. Mol. Biol. 184:147-164(1985) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
[5]	"The 2.7-A crystal structure of deoxygenated hemoglobin from the sea lamprey (Petromyzon marinus): structural basis for a lowered oxygen affinity and Bohr effect." Heaslet H.A., Royer W.E. Jr. Structure 7:517-526(1999) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

PIR

GGLMS. A90676.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1F5O	X-ray	2.90	A/B/C/D/E/F	2-150	[»]
1F5P	X-ray	2.90	A/B/C/D/E/F	2-150	[»]
2LHB	X-ray	2.00	A	2-150	[»]
3LHB	X-ray	2.70	A/B/C/D/E/F/G/H/I/J/K/L	2-150	[»]

ProteinModelPortal

P02208.

SMR

P02208. Positions 2-150.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSPMAT00000005918; ENSPMAP00000005891; ENSPMAG00000005343.
ENSPMAT00000005931; ENSPMAP00000005904; ENSPMAG00000005354.

Phylogenomic databases

GeneTree

ENSGT00650000093060.

HOVERGEN

HBG098500.

Family and domain databases

Gene3D

1.10.490.10. 1 hit.

InterPro

IPR000971. Globin.
IPR009050. Globin-like.
IPR012292. Globin_dom.
IPR013314. Globin_lamprey/hagfish.
[Graphical view]

Pfam

PF00042. Globin. 1 hit.
[Graphical view]

PRINTS

PR01906. FISHGLOBIN.

SUPFAM

SSF46458. Globin_like. 1 hit.

PROSITE

PS01033. GLOBIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P02208.

Entry information

Entry name

GLB5_PETMA

Accession

Primary (citable) accession number: P02208

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program