P02144 (MYG_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Myoglobin

Gene names

Name:

MB

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	154 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.
Sequence similarities	Belongs to the globin family.

Ontologies

Keywords
Biological process	Oxygen transport Transport
Coding sequence diversity	Polymorphism
Ligand	Heme Iron Metal-binding
Molecular function	Muscle protein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	brown fat cell differentiation Inferred from electronic annotation. Source: Compara enucleate erythrocyte differentiation Inferred from electronic annotation. Source: Compara heart development Inferred from electronic annotation. Source: Compara response to hormone stimulus Inferred from electronic annotation. Source: Compara response to hydrogen peroxide Inferred from electronic annotation. Source: Compara response to hypoxia Inferred from electronic annotation. Source: Compara slow-twitch skeletal muscle fiber contraction Inferred from electronic annotation. Source: Compara
Molecular_function	heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro oxygen binding Inferred from electronic annotation. Source: Compara oxygen transporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.8 Ref.11

Chain

2 – 154

153

Myoglobin

PRO_0000053303

Sites

Metal binding

65

1

Iron (heme distal ligand)

Metal binding

94

1

Iron (heme proximal ligand)

Natural variations

Natural variant

55

1

E → K. Ref.12

VAR_003180

Natural variant

134

1

K → N. Ref.14

VAR_003181

Natural variant

140

1

R → Q. Ref.15

VAR_003182

Natural variant

140

1

R → W. Ref.13

VAR_003183

Experimental info

Sequence conflict

106

1

E → Q in AAX84516. Ref.5

Sequence conflict

129

1

Q → E in AAA59595. Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

154

Helix Strand Turn

Details...

Helix

5 – 18

14

Helix

19 – 21

3

Helix

22 – 36

15

Helix

38 – 43

6

Helix

45 – 47

3

Helix

53 – 57

5

Helix

60 – 77

18

Turn

78 – 81

4

Helix

84 – 96

13

Helix

102 – 119

18

Turn

121 – 123

3

Helix

126 – 149

24

Sequences

	Sequence		Length	Mass (Da)
	P02144 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: F6A41F19A525F09C	FASTA	154	17,184
	10 20 30 40 50 60 MGLSDGEWQL VLNVWGKVEA DIPGHGQEVL IRLFKGHPET LEKFDKFKHL KSEDEMKASE 70 80 90 100 110 120 DLKKHGATVL TALGGILKKK GHHEAEIKPL AQSHATKHKI PVKYLEFISE CIIQVLQSKH 130 140 150 PGDFGADAQG AMNKALELFR KDMASNYKEL GFQG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Organization of the human myoglobin gene." Weller P., Jeffreys A.J., Wilson V., Blanchetot A. EMBO J. 3:439-446(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Cloning of the human myoglobin gene." Akaboshi E. Gene 33:241-249(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"A genome annotation-driven approach to cloning the human ORFeome." Collins J.E., Wright C.L., Edwards C.A., Davis M.P., Grinham J.A., Cole C.G., Goward M.E., Aguado B., Mallya M., Mokrab Y., Huckle E.J., Beare D.M., Dunham I. Genome Biol. 5:R84.1-R84.11(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	NIEHS SNPs program Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]	"The DNA sequence of human chromosome 22." Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M., Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C., Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M. , Buck D., Burgess J., Burrill W.D., Burton J., Carder C., Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G., Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V., Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M., Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A., Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C., Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E., Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F., Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M., Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A., Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D., Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y., Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S., Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E., Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L., Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L., Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N., Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A., Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L., Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P., Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P., Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q., Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J., Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J., Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D., Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T., Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P., Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K., Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R., Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L., McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J., Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E., Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P., Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y., Wright H. Nature 402:489-495(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skeletal muscle.
[8]	"Primary structure of human myoglobin." Romero-Herrera A.E., Lehmann H. Nature New Biol. 232:149-152(1971) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-154.
[9]	"The myoglobin of primates. I. Hylobates agilis (gibbon)." Romero-Herrera A.E., Lehmann H. Biochim. Biophys. Acta 251:482-488(1971) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION TO 20-23 AND 84.
[10]	"The myoglobin of primates. II. Pan troglodytes (chimpanzee)." Romero-Herrera A.E., Lehmann H. Biochim. Biophys. Acta 278:62-67(1972) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION TO 100-102.
[11]	"The human myocardial two-dimensional gel protein database: update 1994." Corbett J.M., Wheeler C.H., Baker C.S., Yacoub M.H., Dunn M.J. Electrophoresis 15:1459-1465(1994) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-21. Tissue: Heart.
[12]	"Abnormal human myoglobin: 53 (D4) glutamic acid-->lysine." Boulton F.E., Huntsman R.G., Lorkin P.A., Lehmann H. Nature 223:832-833(1969) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT LYS-55.
[13]	"The third variant of human myoglobin showing an unusual amino acid substitution: 138(H16)arginine-->tryptophan." Boulton F.E., Huntsman R.G., Romero Herrera A., Lorkin P.A., Lehmann H. Biochim. Biophys. Acta 229:716-719(1971) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT TRP-140.
[14]	"A human myoglobin variant 133 (H-10)lysine-->asparagine." Boulton F.E., Huntsman R.G., Romero Herrera A.E., Lorkin P.A., Lehmann H. Biochim. Biophys. Acta 229:871-876(1971) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT ASN-134.
[15]	"The second variant of human myoglobin; 138(H16) arginine leads to glutamine." Boulton F.E., Huntsman R.G., Yawson G.I., Romero-Herrera A.E., Lorkin P.A. Br. J. Haematol. 20:69-74(1971) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT GLN-140.
[16]	"X-ray crystal structure of a recombinant human myoglobin mutant at 2.8-A resolution." Hubbard S.R., Hendrickson W.A., Lambright D.G., Boxer S.G. J. Mol. Biol. 213:215-218(1990) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF MUTANT ARG-46 AND ALA-111 IN COMPLEX WITH HEME.
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X00371, X00372, X00373 Genomic DNA. Translation: CAA25109.1.
M14603, M10090, M14602 Genomic DNA. Translation: AAA59595.1.
CR456516 mRNA. Translation: CAG30402.1.
CR541949 mRNA. Translation: CAG46747.1.
DQ003030 Genomic DNA. Translation: AAX84516.1.
AL022334, AL049747 Genomic DNA. Translation: CAI21837.1.
BC014547 mRNA. Translation: AAH14547.1.

IPI

IPI00217493.

PIR

MYHU. I53991.

RefSeq

NP_005359.1. NM_005368.2.
NP_976311.1. NM_203377.1.
NP_976312.1. NM_203378.1.

UniGene

Hs.517586.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3RGK	X-ray	1.65	A	2-154	[»]

ProteinModelPortal

P02144.

ModBase

Search...

Protein-protein interaction databases

STRING

9606.ENSP00000352835.

PTM databases

PhosphoSite

P02144.

Polymorphism databases

DMDM

127661.

2D gel databases

UCD-2DPAGE

P02144.

Proteomic databases

PaxDb

P02144.

PeptideAtlas

P02144.

PRIDE

P02144.

Protocols and materials databases

DNASU

4151.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000359787; ENSP00000352835; ENSG00000198125.
ENST00000397326; ENSP00000380489; ENSG00000198125.
ENST00000397328; ENSP00000380491; ENSG00000198125.
ENST00000406324; ENSP00000384239; ENSG00000198125.

GeneID

4151.

KEGG

hsa:4151.

UCSC

uc003anz.3. human.

Organism-specific databases

CTD

4151.

GeneCards

GC22M036002.

HGNC

HGNC:6915. MB.

HPA

CAB000060.
HPA003123.

MIM

160000. gene.

neXtProt

NX_P02144.

PharmGKB

PA30658.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG276460.

HOGENOM

HOG000070111.

HOVERGEN

HBG107340.

InParanoid

P02144.

OMA

FRNDIAA.

PhylomeDB

P02144.

Gene expression databases

ArrayExpress

P02144.

Bgee

P02144.

CleanEx

HS_MB.

Genevestigator

P02144.

GermOnline

ENSG00000198125. Homo sapiens.

Family and domain databases

Gene3D

1.10.490.10. 1 hit.

InterPro

IPR000971. Globin.
IPR009050. Globin-like.
IPR012292. Globin_dom.
IPR002335. Myoglobin.
[Graphical view]

PANTHER

PTHR11442:SF5. PTHR11442:SF5. 1 hit.

Pfam

PF00042. Globin. 1 hit.
[Graphical view]

PRINTS

PR00613. MYOGLOBIN.

SUPFAM

SSF46458. Globin_like. 1 hit.

PROSITE

PS01033. GLOBIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

MB. human.

GenomeRNAi

4151.

NextBio

16322.

SOURCE

Search...

Entry information

Entry name

MYG_HUMAN

Accession

Primary (citable) accession number: P02144
Secondary accession number(s): Q52H51, Q5THY7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 137 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.