P02089 (HBB2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hemoglobin subunit beta-2

Alternative name(s):
Beta-2-globin
Hemoglobin beta-2 chain
Hemoglobin beta-minor chain

Gene names

Name:

Hbb-b2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	147 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in oxygen transport from the lung to the various peripheral tissues.
Subunit structure	Heterotetramer of two alpha chains and two beta chains.
Tissue specificity	Red blood cells.
Polymorphism	Inbred mouse strains possess 1 of 3 alleles at the HBB locus: D (diffuse), S (single), and P. The D and P alleles are actually closely linked doublets that coordinately express a major and a minor chain, the minor chain being slightly different in the two alleles. The S allele produces only 1 chain, it is characteristic of North American wild mice.
Miscellaneous	The D-minor sequence is shown. See also the entry for the beta D-major chain and the S allele.
Sequence similarities	Belongs to the globin family.

Ontologies

Keywords
Biological process	Oxygen transport Transport
Coding sequence diversity	Polymorphism
Ligand	Heme Iron Metal-binding
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	hemoglobin complex Inferred from electronic annotation. Source: InterPro
Molecular_function	heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro oxygen binding Inferred from electronic annotation. Source: InterPro oxygen transporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 147	146	Hemoglobin subunit beta-2		PRO_0000053025
Sites
	Metal binding	64	1	Iron (heme distal ligand)
	Metal binding	93	1	Iron (heme proximal ligand)
Amino acid modifications
	Modified residue	36	1	Phosphotyrosine Ref.5
	Modified residue	42	1	Phosphotyrosine Ref.6
	Modified residue	45	1	Phosphoserine Ref.6
Natural variations
	Natural variant	23 – 24	2	EV → AI in allele P.

Sequences

	Sequence		Length	Mass (Da)
	P02089 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 1FABBDC2D0ABC4FD	FASTA	147	15,878
	10 20 30 40 50 60 MVHLTDAEKS AVSCLWAKVN PDEVGGEALG RLLVVYPWTQ RYFDSFGDLS SASAIMGNPK 70 80 90 100 110 120 VKAHGKKVIT AFNEGLKNLD NLKGTFASLS ELHCDKLHVD PENFRLLGNA IVIVLGHHLG 130 140 KDFTPAAQAA FQKVVAGVAT ALAHKYH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the BALB/c mouse beta-globin complex." Shehee W.R., Loeb D.D., Adey N.B., Burton F.H., Casavant N.C., Cole P., Davies C.J., McGraw R.A., Schichman S.A., Severynse D.M., Voliva C.F., Weyter F.W., Wisely G.B., Edgell M.H., Hutchison C.A. III J. Mol. Biol. 205:41-62(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"The evolution and sequence comparison of two recently diverged mouse chromosomal beta-globin genes." Konkel D.A., Maizel J.V. Jr., Leder P. Cell 18:865-873(1979) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BALB/c.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT 24-ALA--ILE-25. Strain: Czech II and FVB/N. Tissue: Mammary gland and Salivary gland.
[4]	"Mouse haemoglobin beta chains. Comparative sequence data on adult major and minor beta chains from two species, Mus musculus and Mus cervicolor." Gilman J.G. Biochem. J. 159:43-53(1976) [PubMed] [Europe PMC] [Abstract] Cited for: POLYMORPHISM, VARIANT 23-ALA-ILE-24.
[5]	"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P. J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-36, MASS SPECTROMETRY. Tissue: Brain.
[6]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-42 AND SER-45, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

V00722 Genomic DNA. Translation: CAA24101.1.
X14061 Genomic DNA. Translation: CAA32225.1. Sequence problems.
BC027434 mRNA. Translation: AAH27434.1.
BC032264 mRNA. Translation: AAH32264.1.

IPI

IPI00316491.

PIR

HBMSN1. B90790.

RefSeq

NP_058652.1. NM_016956.2.

UniGene

Mm.288567.
Mm.467412.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1FNG	X-ray	1.90	B/D	65-76	[»]

ProteinModelPortal

P02089.

SMR

P02089. Positions 2-147.

ModBase

Search...

Protein-protein interaction databases

IntAct

P02089. 7 interactions.

PTM databases

PhosphoSite

P02089.

Proteomic databases

PRIDE

P02089.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

15130.

KEGG

mmu:15130.

Organism-specific databases

CTD

15130.

MGI

MGI:96022. Hbb-b2.

Phylogenomic databases

HOVERGEN

HBG009709.

InParanoid

Q5D0E8.

KO

K13823.

Gene expression databases

Genevestigator

P02089.

GermOnline

ENSMUSG00000052305. Mus musculus.

Family and domain databases

Gene3D

1.10.490.10. 1 hit.

InterPro

IPR000971. Globin.
IPR009050. Globin-like.
IPR012292. Globin_dom.
IPR002337. Haemoglobin_b.
[Graphical view]

Pfam

PF00042. Globin. 1 hit.
[Graphical view]

PRINTS

PR00814. BETAHAEM.

SUPFAM

SSF46458. Globin_like. 1 hit.

PROSITE

PS01033. GLOBIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChEMBL

CHEMBL4007.

EvolutionaryTrace

P02089.

NextBio

287586.

SOURCE

Search...

Entry information

Entry name

HBB2_MOUSE

Accession

Primary (citable) accession number: P02089
Secondary accession number(s): Q5D0E8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 116 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program