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Protein

Hemoglobin subunit beta

Gene

HBB

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in oxygen transport from the lung to the various peripheral tissues.1 Publication
Spinorphin: functions as an endogenous inhibitor of enkephalin-degrading enzymes such as DPP3, and may thereby play a role as a regulator of pain and inflammation.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi62 – 621Iron (heme distal ligand)
Metal bindingi91 – 911Iron (heme proximal ligand)

GO - Molecular functioni

Complete GO annotation...

Keywords - Biological processi

Oxygen transport, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiR-BTA-983231. Factors involved in megakaryocyte development and platelet production.

Names & Taxonomyi

Protein namesi
Recommended name:
Hemoglobin subunit beta
Alternative name(s):
Beta-globin
Hemoglobin beta chain
Cleaved into the following chain:
Gene namesi
Name:HBB
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 15

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Protein family/group databases

Allergomei8242. Bos d HG.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 145145Hemoglobin subunit betaPRO_0000052892Add
BLAST
Peptidei31 – 377SpinorphinPRO_0000424225

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei11 – 111PhosphothreonineBy similarity
Modified residuei43 – 431PhosphoserineBy similarity
Modified residuei58 – 581N6-acetyllysineBy similarity
Modified residuei81 – 811N6-acetyllysineBy similarity
Modified residuei92 – 921S-nitrosocysteineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein, S-nitrosylation

Proteomic databases

PaxDbiP02070.
PeptideAtlasiP02070.
PRIDEiP02070.

Miscellaneous databases

PMAP-CutDBP02070.

Expressioni

Tissue specificityi

Red blood cells.

Gene expression databases

ExpressionAtlasiP02070. baseline and differential.

Interactioni

Subunit structurei

Heterotetramer of two alpha chains and two beta chains.

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000043063.

Structurei

Secondary structure

1
145
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 1411Combined sources
Helixi21 – 3313Combined sources
Helixi35 – 4410Combined sources
Helixi50 – 556Combined sources
Helixi57 – 7317Combined sources
Helixi77 – 793Combined sources
Helixi80 – 834Combined sources
Helixi85 – 939Combined sources
Helixi100 – 11718Combined sources
Helixi118 – 1203Combined sources
Helixi123 – 14018Combined sources
Helixi141 – 1444Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FSXX-ray2.10B/D1-145[»]
1G08X-ray1.90B/D1-145[»]
1G09X-ray2.04B/D1-145[»]
1G0AX-ray2.04B/D1-145[»]
1HDAX-ray2.20B/D1-145[»]
2QSPX-ray1.85B/D1-145[»]
2QSSX-ray1.75B/D1-145[»]
3CIUX-ray3.50B/D1-145[»]
3PI8X-ray2.20B/D1-145[»]
3PI9X-ray2.90B/D1-145[»]
3PIAX-ray2.10B/D1-145[»]
ProteinModelPortaliP02070.
SMRiP02070. Positions 1-145.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP02070.

Family & Domainsi

Sequence similaritiesi

Belongs to the globin family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3378. Eukaryota.
COG1018. LUCA.
GeneTreeiENSGT00760000119197.
HOGENOMiHOG000036868.
HOVERGENiHBG009709.
InParanoidiP02070.
KOiK13823.
OMAiWTRRFFE.
OrthoDBiEOG7B8S5H.
TreeFamiTF333268.

Family and domain databases

Gene3Di1.10.490.10. 1 hit.
InterProiIPR000971. Globin.
IPR009050. Globin-like.
IPR012292. Globin/Proto.
IPR002337. Haemoglobin_b.
[Graphical view]
PfamiPF00042. Globin. 1 hit.
[Graphical view]
PRINTSiPR00814. BETAHAEM.
SUPFAMiSSF46458. SSF46458. 1 hit.
PROSITEiPS01033. GLOBIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02070-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLTAEEKAAV TAFWGKVKVD EVGGEALGRL LVVYPWTQRF FESFGDLSTA
60 70 80 90 100
DAVMNNPKVK AHGKKVLDSF SNGMKHLDDL KGTFAALSEL HCDKLHVDPE
110 120 130 140
NFKLLGNVLV VVLARNFGKE FTPVLQADFQ KVVAGVANAL AHRYH
Length:145
Mass (Da):15,954
Last modified:July 21, 1986 - v1
Checksum:iFD62217E8477CFD4
GO

Polymorphismi

Four allelic beta chains have been found in bovine hemoglobins. A and B alleles were found in Jersey cattle and C and D alleles were found in Angoni cattle (East African short-horn zebu). The sequence shown is that of the allele A.3 Publications

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti15 – 151G → S in allele B. 1 Publication
Natural varianti18 – 181K → H in allele B. 1 Publication
Natural varianti20 – 201D → G in allele D-Zambia. 1 Publication
Natural varianti43 – 431S → T in allele D-Zambia. 1 Publication
Natural varianti119 – 1191K → N in allele B.
Natural varianti131 – 1311K → Q in allele C-Rhodesia. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00376 Genomic DNA. Translation: CAA25111.1.
M63453 Genomic DNA. Translation: AAA30408.1.
PIRiB93504. HBBOB.
RefSeqiNP_776342.1. NM_173917.2.
UniGeneiBt.23726.

Genome annotation databases

EnsembliENSBTAT00000045694; ENSBTAP00000043063; ENSBTAG00000038748.
GeneIDi280813.
KEGGibta:280813.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Worthington enzyme manual

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00376 Genomic DNA. Translation: CAA25111.1.
M63453 Genomic DNA. Translation: AAA30408.1.
PIRiB93504. HBBOB.
RefSeqiNP_776342.1. NM_173917.2.
UniGeneiBt.23726.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FSXX-ray2.10B/D1-145[»]
1G08X-ray1.90B/D1-145[»]
1G09X-ray2.04B/D1-145[»]
1G0AX-ray2.04B/D1-145[»]
1HDAX-ray2.20B/D1-145[»]
2QSPX-ray1.85B/D1-145[»]
2QSSX-ray1.75B/D1-145[»]
3CIUX-ray3.50B/D1-145[»]
3PI8X-ray2.20B/D1-145[»]
3PI9X-ray2.90B/D1-145[»]
3PIAX-ray2.10B/D1-145[»]
ProteinModelPortaliP02070.
SMRiP02070. Positions 1-145.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000043063.

Protein family/group databases

Allergomei8242. Bos d HG.

Proteomic databases

PaxDbiP02070.
PeptideAtlasiP02070.
PRIDEiP02070.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000045694; ENSBTAP00000043063; ENSBTAG00000038748.
GeneIDi280813.
KEGGibta:280813.

Organism-specific databases

CTDi3043.

Phylogenomic databases

eggNOGiKOG3378. Eukaryota.
COG1018. LUCA.
GeneTreeiENSGT00760000119197.
HOGENOMiHOG000036868.
HOVERGENiHBG009709.
InParanoidiP02070.
KOiK13823.
OMAiWTRRFFE.
OrthoDBiEOG7B8S5H.
TreeFamiTF333268.

Enzyme and pathway databases

ReactomeiR-BTA-983231. Factors involved in megakaryocyte development and platelet production.

Miscellaneous databases

EvolutionaryTraceiP02070.
PMAP-CutDBP02070.

Gene expression databases

ExpressionAtlasiP02070. baseline and differential.

Family and domain databases

Gene3Di1.10.490.10. 1 hit.
InterProiIPR000971. Globin.
IPR009050. Globin-like.
IPR012292. Globin/Proto.
IPR002337. Haemoglobin_b.
[Graphical view]
PfamiPF00042. Globin. 1 hit.
[Graphical view]
PRINTSiPR00814. BETAHAEM.
SUPFAMiSSF46458. SSF46458. 1 hit.
PROSITEiPS01033. GLOBIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Ruminant globin gene structures suggest an evolutionary role for Alu-type repeats."
    Schimenti J.C., Duncan C.H.
    Nucleic Acids Res. 12:1641-1655(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ALLELE A).
  2. "A comparison of amino acid sequences in the beta-chains of adult bovine hemoglobins A and B."
    Schroeder W.A., Shelton J.R., Shelton J.B., Robberson B., Babin D.R.
    Arch. Biochem. Biophys. 120:124-135(1967) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE (ALLELES A AND B).
  3. "Isolation and identification of an endogenous inhibitor of enkephalin-degrading enzymes from bovine spinal cord."
    Nishimura K., Hazato T.
    Biochem. Biophys. Res. Commun. 194:713-719(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 31-37, FUNCTION OF SPINORPHIN.
  4. "Amino acid sequences in the beta-chains of adult bovine hemoglobins C-Rhodesia and D-Zambia."
    Schroeder W.A., Shelton J.R., Shelton J.B., Apell G., Huisman T.H.J., Smith L.L., Carr W.R.
    Arch. Biochem. Biophys. 152:222-232(1972) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE (ALLELES C-RHODESIA AND D-ZAMBIA).
  5. "A novel allosteric mechanism in haemoglobin. Structure of bovine deoxyhaemoglobin, absence of specific chloride-binding sites and origin of the chloride-linked Bohr effect in bovine and human haemoglobin."
    Perutz M.F., Fermi G., Poyart C., Pagnier J., Kister J.
    J. Mol. Biol. 233:536-545(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
  6. "The X-ray structure determination of bovine carbonmonoxy hemoglobin at 2.1 A resoultion and its relationship to the quaternary structures of other hemoglobin crystal froms."
    Safo M.K., Abraham D.J.
    Protein Sci. 10:1091-1099(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).

Entry informationi

Entry nameiHBB_BOVIN
AccessioniPrimary (citable) accession number: P02070
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: July 6, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.