Reviewed, UniProtKB/Swiss-Prot P01959 (HBA_EQUAS)

Names and origin

Protein names

Recommended name:
    Hemoglobin subunit alpha
Alternative name(s):
    Hemoglobin alpha chain
    Alpha-globin

Gene names

Name:	HBA1
AND
Name:	HBA2

Organism

Equus asinus (Donkey)

Taxonomic identifier

9793 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Perissodactyla › Equidae › Equus

Protein attributes

Sequence length	142 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in oxygen transport from the lung to the various peripheral tissues.
Subunit structure	Heterotetramer of two alpha chains and two beta chains.
Tissue specificity	Red blood cells.
Sequence similarities	Belongs to the globin family.

Ontologies

Keywords
Biological process	Oxygen transport Transport
Ligand	Heme Iron Metal-binding
PTM	Acetylation
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	oxygen transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	hemoglobin complex Inferred from electronic annotation. Source: InterPro
Molecular function	heme binding Inferred from electronic annotation. Source: InterPro oxygen binding Inferred from electronic annotation. Source: InterPro oxygen transporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.2

Chain

2 – 142

141

Hemoglobin subunit alpha

PRO_0000052624

Sites

Metal binding

59

1

Iron (heme distal ligand)

Metal binding

88

1

Iron (heme proximal ligand)

Amino acid modifications

Modified residue

17

1

N6-acetyllysine By similarity

Experimental info

Sequence conflict

132

1

T → S AA sequence Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

142

Helix Strand Turn

Details...

Helix

5 – 18

14

Helix

19 – 21

3

Helix

22 – 36

15

Helix

38 – 43

6

Helix

54 – 72

19

Helix

74 – 76

3

Helix

77 – 80

4

Helix

82 – 88

7

Turn

89 – 92

4

Helix

97 – 113

17

Turn

115 – 117

3

Helix

120 – 136

17

Turn

137 – 141

5

Sequences

	Sequence		Length	Mass (Da)
	P01959-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 93B837BC15C1D175	FASTA	142	15,220
	10 20 30 40 50 60 MVLSAADKTN VKAAWSKVGG NAGEFGAEAL ERMFLGFPTT KTYFPHFDLS HGSAQVKAHG 70 80 90 100 110 120 KKVGDALTLA VGHLDDLPGA LSNLSDLHAH KLRVDPVNFK LLSHCLLSTL AVHLPNDFTP 130 140 AVHASLDKFL STVSTVLTSK YR

References

[1]	"Phylogenetic relationships within the genus Equus and the evolution of alpha and theta globin genes." Oakenfull E.A., Clegg J.B. J. Mol. Evol. 47:772-783(1998) [PubMed: 9847419] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (HBA1 AND HBA2).
[2]	"Amino-acid replacements in horse haemoglobin." Kilmartin J.V., Clegg J.B. Nature 213:269-271(1967) [PubMed: 6030605] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-142.
[3]	"Crystal structure of haemoglobin from donkey (Equus asinus) at 3A resolution." Balasundaresan D., Saraboji K., Ponnuswamy M.N. Biochimie 88:719-723(2006) [PubMed: 16488065] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).

Cross-references

Sequence databases

EMBLGenBankDDBJ

U69894 Genomic DNA. Translation: AAD13640.1.
U69895 Genomic DNA. Translation: AAD13641.1.

PIR

HAHOD. A02282.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1S0H	X-ray	3.00	A	2-142	[»]

ModBase

Search...

Phylogenomic databases

HOVERGEN

P01959.

Family and domain databases

InterPro

IPR000971. Globin_subset.
IPR002338. Haemoglobin_a.
IPR018331. Haemoglobin_alpha_chain.
IPR002339. Haemoglobin_pi.
[Graphical view]

PANTHER

PTHR11442:SF14. Pi_haem. 1 hit.

Pfam

PF00042. Globin. 1 hit.
[Graphical view]

PRINTS

PR00612. ALPHAHAEM.
PR00815. PIHAEM.

PROSITE

PS01033. GLOBIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

HBA_EQUAS

Accession

Primary (citable) accession number: P01959
Secondary accession number(s): P82988

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	October 13, 2009
This is version 71 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)