P01958 (HBA_HORSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hemoglobin subunit alpha

Alternative name(s):
Alpha-globin
Hemoglobin alpha chain

Gene names

Name:

HBA

Organism

Equus caballus (Horse) [Reference proteome]

Taxonomic identifier

9796 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Perissodactyla › Equidae › Equus › Equus

Protein attributes

Sequence length	142 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in oxygen transport from the lung to the various peripheral tissues.
Subunit structure	Heterotetramer of two alpha chains and two beta chains.
Tissue specificity	Red blood cells.
Polymorphism	Horse has two non-allelic alpha chains, slow and fast. The slow chain sequence is shown.
Sequence similarities	Belongs to the globin family.

Ontologies

Keywords
Biological process	Oxygen transport Transport
Coding sequence diversity	Polymorphism
Ligand	Heme Iron Metal-binding
PTM	Acetylation
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	hemoglobin complex Inferred from electronic annotation. Source: InterPro
Molecular_function	heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro oxygen binding Inferred from electronic annotation. Source: InterPro oxygen transporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.3

Chain

2 – 142

141

Hemoglobin subunit alpha

PRO_0000052652

Sites

Metal binding

59

1

Iron (heme distal ligand)

Metal binding

88

1

Iron (heme proximal ligand)

Amino acid modifications

Modified residue

17

1

N6-acetyllysine By similarity

Natural variations

Natural variant

25

1

Y → F Exist in both chains.

Natural variant

61

1

K → Q in fast chain.

Secondary structure

1

.

.

.

.

.

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.

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.

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.

.

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142

Helix Strand Turn

Details...

Helix

5 – 17

13

Turn

18 – 20

3

Helix

22 – 36

15

Helix

38 – 43

6

Helix

46 – 50

5

Helix

54 – 71

18

Helix

72 – 76

5

Helix

77 – 80

4

Helix

82 – 90

9

Helix

96 – 113

18

Turn

115 – 117

3

Helix

120 – 137

18

Turn

138 – 140

3

Sequences

	Sequence		Length	Mass (Da)
	P01958 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 33BB917E15C9CD15	FASTA	142	15,245
	10 20 30 40 50 60 MVLSAADKTN VKAAWSKVGG HAGEYGAEAL ERMFLGFPTT KTYFPHFDLS HGSAQVKAHG 70 80 90 100 110 120 KKVGDALTLA VGHLDDLPGA LSNLSDLHAH KLRVDPVNFK LLSHCLLSTL AVHLPNDFTP 130 140 AVHASLDKFL SSVSTVLTSK YR

References

[1]	"Genetic organization of the polymorphic equine alpha globin locus and sequence of the BII alpha 1 gene." Clegg J.B., Goodbourn S.E.Y., Braend M. Nucleic Acids Res. 12:7847-7858(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Gene conversions in the horse alpha-globin gene complex." Clegg J.B. Mol. Biol. Evol. 4:492-503(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Hemoglobins, XXXIII. Note on the sequence of the hemoglobins of the horse." Matsuda G., Maita T., Braunitzer G., Schrank B. Hoppe-Seyler's Z. Physiol. Chem. 361:1107-1116(1980) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-142.
[4]	"A correction to the sequence of the alpha chains of horse haemoglobin." Ladner R.C., Air G.M., Fogg J.H. J. Mol. Biol. 103:675-677(1976) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION.
[5]	"Three-dimensional Fourier synthesis of horse oxyhaemoglobin at 2.8-A resolution: (1) X-ray analysis." Perutz M.F., Miurhead H., Cox J.M., Goaman L.C., Mathews F.S., McGandy E.L., Webb L.E. Nature 219:29-32(1968) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
[6]	"Three-dimensional Fourier synthesis of horse oxyhaemoglobin at 2.8 A resolution: the atomic model." Perutz M.F., Muirhead H., Cox J.M., Goaman L.C. Nature 219:131-139(1968) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
[7]	"The structure of horse methaemoglobin at 2.0-A resolution." Ladner R.C., Heidner E.J., Perutz M.F. J. Mol. Biol. 114:385-414(1977) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
+	Additional computationally mapped references.

Web resources

Protein Spotlight

The man behind the molecular lung - Issue 21 of April 2002

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X01086 Genomic DNA. Translation: CAA25564.1.
M17902 Genomic DNA. Translation: AAD15306.1.
M17903 Genomic DNA. Translation: AAA30948.1.
X07053 Genomic DNA. Translation: CAA30097.1.

PIR

HAHO. I46302.

RefSeq

NP_001078901.1. NM_001085432.1.

UniGene

Eca.11653.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1G0B	X-ray	1.90	A	2-141	[»]
1IBE	X-ray	1.80	A	2-141	[»]
1IWH	X-ray	1.55	A	2-142	[»]
1NS6	X-ray	2.05	A	2-141	[»]
1NS9	X-ray	1.60	A	2-141	[»]
1Y8H	X-ray	3.10	A/C	2-141	[»]
1Y8I	X-ray	2.60	A/C	2-141	[»]
1Y8K	X-ray	2.30	A/C	2-141	[»]
2D5X	X-ray	1.45	A	2-141	[»]
2DHB	X-ray	2.80	A	2-141	[»]
2MHB	X-ray	2.00	A	2-142	[»]
2ZLT	X-ray	1.90	A	2-142	[»]
2ZLU	X-ray	2.00	A	2-142	[»]
2ZLV	X-ray	2.00	A	2-142	[»]
2ZLW	X-ray	2.90	A/C	2-142	[»]
2ZLX	X-ray	2.80	A/C	2-142	[»]

ProteinModelPortal

P01958.

SMR

P01958. Positions 2-142.

ModBase

Search...

Protein-protein interaction databases

MINT

MINT-242180.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

100036557.

KEGG

ecb:100036557.

Organism-specific databases

CTD

15121.

Phylogenomic databases

HOVERGEN

HBG009709.

KO

K13822.

Family and domain databases

Gene3D

1.10.490.10. 1 hit.

InterPro

IPR000971. Globin.
IPR009050. Globin-like.
IPR012292. Globin_dom.
IPR002338. Haemoglobin_a.
IPR018331. Haemoglobin_alpha_chain.
IPR002339. Haemoglobin_pi.
[Graphical view]

PANTHER

PTHR11442:SF14. PTHR11442:SF14. 1 hit.

Pfam

PF00042. Globin. 1 hit.
[Graphical view]

PRINTS

PR00612. ALPHAHAEM.
PR00815. PIHAEM.

SUPFAM

SSF46458. Globin_like. 1 hit.

PROSITE

PS01033. GLOBIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P01958.

Entry information

Entry name

HBA_HORSE

Accession

Primary (citable) accession number: P01958
Secondary accession number(s): Q28384

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	May 29, 2013
This is version 114 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program