Reviewed, UniProtKB/Swiss-Prot P01958 (HBA_HORSE)

Names and origin

Protein names

Recommended name:
    Hemoglobin subunit alpha
Alternative name(s):
    Hemoglobin alpha chain
    Alpha-globin

Gene names

Name:

HBA

Organism

Equus caballus (Horse)

Taxonomic identifier

9796 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Perissodactyla › Equidae › Equus

Protein attributes

Sequence length	142 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in oxygen transport from the lung to the various peripheral tissues.
Subunit structure	Heterotetramer of two alpha chains and two beta chains.
Tissue specificity	Red blood cells.
Polymorphism	Horse has two non-allelic alpha chains, slow and fast. The slow chain sequence is shown.
Sequence similarities	Belongs to the globin family.

Ontologies

Keywords
Biological process	Oxygen transport Transport
Coding sequence diversity	Polymorphism
Ligand	Heme Iron Metal-binding
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	oxygen transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	hemoglobin complex Inferred from electronic annotation. Source: InterPro
Molecular function	heme binding Inferred from electronic annotation. Source: InterPro oxygen binding Inferred from electronic annotation. Source: InterPro oxygen transporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.3

Chain

2 – 142

141

Hemoglobin subunit alpha

PRO_0000052652

Sites

Metal binding

59

1

Iron (heme distal ligand)

Metal binding

88

1

Iron (heme proximal ligand)

Natural variations

Natural variant

25

1

Y → F Exist in both chains.

Natural variant

61

1

K → Q in fast chain.

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

142

Helix Strand Turn

Details...

Helix

5 – 17

13

Turn

18 – 20

3

Helix

22 – 36

15

Helix

38 – 43

6

Helix

54 – 71

18

Helix

72 – 76

5

Helix

77 – 80

4

Helix

82 – 90

9

Helix

96 – 113

18

Turn

115 – 117

3

Helix

120 – 137

18

Turn

138 – 140

3

Sequences

	Sequence		Length	Mass (Da)
	P01958-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 33BB917E15C9CD15	FASTA	142	15,245
	10 20 30 40 50 60 MVLSAADKTN VKAAWSKVGG HAGEYGAEAL ERMFLGFPTT KTYFPHFDLS HGSAQVKAHG 70 80 90 100 110 120 KKVGDALTLA VGHLDDLPGA LSNLSDLHAH KLRVDPVNFK LLSHCLLSTL AVHLPNDFTP 130 140 AVHASLDKFL SSVSTVLTSK YR

References

[1]	"Genetic organization of the polymorphic equine alpha globin locus and sequence of the BII alpha 1 gene." Clegg J.B., Goodbourn S.E.Y., Braend M. Nucleic Acids Res. 12:7847-7858(1984) [PubMed: 6093055] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Gene conversions in the horse alpha-globin gene complex." Clegg J.B. Mol. Biol. Evol. 4:492-503(1987) [PubMed: 2835578] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Hemoglobins, XXXIII. Note on the sequence of the hemoglobins of the horse." Matsuda G., Maita T., Braunitzer G., Schrank B. Hoppe-Seyler's Z. Physiol. Chem. 361:1107-1116(1980) [PubMed: 7409745] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-142.
[4]	"A correction to the sequence of the alpha chains of horse haemoglobin." Ladner R.C., Air G.M., Fogg J.H. J. Mol. Biol. 103:675-677(1976) [PubMed: 940162] [Abstract] Cited for: SEQUENCE REVISION.
[5]	"Three-dimensional Fourier synthesis of horse oxyhaemoglobin at 2.8-A resolution: (1) X-ray analysis." Perutz M.F., Miurhead H., Cox J.M., Goaman L.C., Mathews F.S., McGandy E.L., Webb L.E. Nature 219:29-32(1968) [PubMed: 5659617] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
[6]	"Three-dimensional Fourier synthesis of horse oxyhaemoglobin at 2.8 A resolution: the atomic model." Perutz M.F., Muirhead H., Cox J.M., Goaman L.C. Nature 219:131-139(1968) [PubMed: 5659637] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
[7]	"The structure of horse methaemoglobin at 2.0-A resolution." Ladner R.C., Heidner E.J., Perutz M.F. J. Mol. Biol. 114:385-414(1977) [PubMed: 561852] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
+	Additional computationally mapped references.

Web resources

Protein Spotlight

The man behind the molecular lung - Issue 21 of April 2002

Cross-references

Sequence databases

EMBLGenBankDDBJ

X01086 Genomic DNA. Translation: CAA25564.1.
M17902 Genomic DNA. Translation: AAD15306.1.
M17903 Genomic DNA. Translation: AAA30948.1.
X07053 Genomic DNA. Translation: CAA30097.1.

PIR

HAHO. I46302.

UniGene

Eca.11653
Eca.15779

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1G0B	X-ray	1.90	A	2-141	[»]
1IBE	X-ray	1.80	A	2-141	[»]
1IWH	X-ray	1.55	A	2-141	[»]
1NS6	X-ray	2.05	A	2-141	[»]
1NS9	X-ray	1.60	A	2-141	[»]
1Y8H	X-ray	3.10	A/C	2-141	[»]
1Y8I	X-ray	2.60	A/C	2-141	[»]
1Y8K	X-ray	2.30	A/C	2-141	[»]
2D5X	X-ray	1.45	A	2-141	[»]
2DHB	X-ray	2.80	A	2-141	[»]
2MHB	X-ray	2.00	A	2-142	[»]
2ZLT	X-ray	1.90	A	2-142	[»]
2ZLU	X-ray	2.00	A	2-142	[»]
2ZLV	X-ray	2.00	A	2-142	[»]
2ZLW	X-ray	2.90	A/C	2-142	[»]
2ZLX	X-ray	2.80	A/C	2-142	[»]

ModBase

Search...

Phylogenomic databases

HOVERGEN

P01958.

Family and domain databases

InterPro

IPR000971. Globin_subset.
IPR002338. Haemoglobin_a.
IPR018331. Haemoglobin_alpha_chain.
IPR002339. Haemoglobin_pi.
[Graphical view]

PANTHER

PTHR11442:SF14. Pi_haem. 1 hit.

Pfam

PF00042. Globin. 1 hit.
[Graphical view]

PRINTS

PR00612. ALPHAHAEM.
PR00815. PIHAEM.

PROSITE

PS01033. GLOBIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

HBA_HORSE

Accession

Primary (citable) accession number: P01958
Secondary accession number(s): Q28384

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)