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Protein

H-2 class II histocompatibility antigen, A-K alpha chain

Gene

H2-Aa

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  • peptide antigen binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Immunity

Names & Taxonomyi

Protein namesi
Recommended name:
H-2 class II histocompatibility antigen, A-K alpha chain
Gene namesi
Name:H2-Aa
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:95895. H2-Aa.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini24 – 218195ExtracellularSequence analysisAdd
BLAST
Transmembranei219 – 24123HelicalSequence analysisAdd
BLAST
Topological domaini242 – 25615CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • external side of plasma membrane Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • lysosome Source: MGI
  • membrane Source: MGI
  • MHC class II protein complex Source: MGI
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane, MHC II

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323By similarityAdd
BLAST
Chaini24 – 256233H-2 class II histocompatibility antigen, A-K alpha chainPRO_0000018977Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi105 – 1051N-linked (GlcNAc...)1 Publication
Disulfide bondi134 ↔ 190PROSITE-ProRule annotation
Glycosylationi145 – 1451N-linked (GlcNAc...)1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiP01910.
PaxDbiP01910.
PRIDEiP01910.

Expressioni

Gene expression databases

CleanExiMM_H2-AA.

Interactioni

Protein-protein interaction databases

DIPiDIP-6133N.
IntActiP01910. 1 interaction.
MINTiMINT-1533544.
STRINGi10090.ENSMUSP00000046105.

Structurei

Secondary structure

1
256
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi30 – 4213Combined sources
Turni43 – 453Combined sources
Beta strandi46 – 538Combined sources
Beta strandi56 – 627Combined sources
Turni63 – 664Combined sources
Beta strandi67 – 726Combined sources
Helixi73 – 775Combined sources
Helixi83 – 10422Combined sources
Beta strandi115 – 1228Combined sources
Beta strandi126 – 1283Combined sources
Beta strandi130 – 13910Combined sources
Beta strandi145 – 1506Combined sources
Beta strandi152 – 1554Combined sources
Beta strandi159 – 1613Combined sources
Beta strandi172 – 1809Combined sources
Beta strandi188 – 1936Combined sources
Beta strandi201 – 2055Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1D9KX-ray3.20C/G27-209[»]
1IAKX-ray1.90A24-222[»]
1JL4X-ray4.30A31-208[»]
ProteinModelPortaliP01910.
SMRiP01910. Positions 27-208.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP01910.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini114 – 20693Ig-like C1-typeAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni24 – 11188Alpha-1Add
BLAST
Regioni112 – 20594Alpha-2Add
BLAST
Regioni206 – 21813Connecting peptideAdd
BLAST

Sequence similaritiesi

Belongs to the MHC class II family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IZMF. Eukaryota.
ENOG410YHX9. LUCA.
HOVERGENiHBG006862.
InParanoidiP01910.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.10.320.10. 1 hit.
InterProiIPR032431. C1-set_C.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR011162. MHC_I/II-like_Ag-recog.
IPR014745. MHC_II_a/b_N.
IPR001003. MHC_II_a_N.
[Graphical view]
PfamiPF07654. C1-set. 1 hit.
PF16196. C1-set_C. 1 hit.
PF00993. MHC_II_alpha. 1 hit.
[Graphical view]
SMARTiSM00407. IGc1. 1 hit.
SM00920. MHC_II_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF54452. SSF54452. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01910-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRSRALILG VLALTTMLSL CGGEDDIEAD HVGSYGITVY QSPGDIGQYT
60 70 80 90 100
FEFDGDELFY VDLDKKETVW MLPEFAQLRR FEPQGGLQNI ATGKHNLEIL
110 120 130 140 150
TKRSNSTPAT NEAPQATVFP KSPVLLGQPN TLICFVDNIF PPVINITWLR
160 170 180 190 200
NSKSVTDGVY ETSFFVNRDY SFHKLSYLTF IPSDDDIYDC KVEHWGLEEP
210 220 230 240 250
VLKHWEPEIP APMSELTETV VCALGLSVGL VGIVVGTIFI IQGLRSGGTS

RHPGPL
Length:256
Mass (Da):28,351
Last modified:October 1, 1996 - v2
Checksum:i78E7D4A33CCE4582
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21931 mRNA. Translation: AAA39636.1.
V00832 mRNA. Translation: CAA24215.1.
M11357 mRNA. Translation: AAA39613.1.
PIRiA02217. HLMSAA.
I54447.
UniGeneiMm.235338.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21931 mRNA. Translation: AAA39636.1.
V00832 mRNA. Translation: CAA24215.1.
M11357 mRNA. Translation: AAA39613.1.
PIRiA02217. HLMSAA.
I54447.
UniGeneiMm.235338.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1D9KX-ray3.20C/G27-209[»]
1IAKX-ray1.90A24-222[»]
1JL4X-ray4.30A31-208[»]
ProteinModelPortaliP01910.
SMRiP01910. Positions 27-208.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-6133N.
IntActiP01910. 1 interaction.
MINTiMINT-1533544.
STRINGi10090.ENSMUSP00000046105.

Proteomic databases

EPDiP01910.
PaxDbiP01910.
PRIDEiP01910.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:95895. H2-Aa.

Phylogenomic databases

eggNOGiENOG410IZMF. Eukaryota.
ENOG410YHX9. LUCA.
HOVERGENiHBG006862.
InParanoidiP01910.

Miscellaneous databases

EvolutionaryTraceiP01910.
SOURCEiSearch...

Gene expression databases

CleanExiMM_H2-AA.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.10.320.10. 1 hit.
InterProiIPR032431. C1-set_C.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR011162. MHC_I/II-like_Ag-recog.
IPR014745. MHC_II_a/b_N.
IPR001003. MHC_II_a_N.
[Graphical view]
PfamiPF07654. C1-set. 1 hit.
PF16196. C1-set_C. 1 hit.
PF00993. MHC_II_alpha. 1 hit.
[Graphical view]
SMARTiSM00407. IGc1. 1 hit.
SM00920. MHC_II_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF54452. SSF54452. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Signaling to a B-cell clone by Ek, but not Ak, does not reflect alteration of Ak genes."
    Bishop G.A., McMillan M.S., Haughton G., Frelinger J.A.
    Immunogenetics 28:184-192(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The murine Ia alpha chains, E alpha and A alpha, show a surprising degree of sequence homology."
    Benoist C.O., Mathis D.J., Kanter M.R., Williams V.E., McDevitt H.O.
    Proc. Natl. Acad. Sci. U.S.A. 80:534-538(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Comparative sequence analysis of cDNA clones encoding I-A molecules of the CH12 B cell lymphoma: nucleotide differences do not account for their 'defective' function in B cell stimulation."
    Sharma S., King L.B., Corley R.B., Maki R.
    Immunol. Invest. 16:425-436(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 16-256.
  4. "A molecular basis for the Ia.2 and Ia.19 antigenic determinants."
    Landais D., Matthes H., Benoist C., Mathis D.
    Proc. Natl. Acad. Sci. U.S.A. 82:2930-2934(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 24-256.
  5. "Oligosaccharide microheterogeneity of the murine major histocompatibility antigens. Reproducible site-specific patterns of sialylation and branching in asparagine-linked oligosaccharides."
    Swiedler S.J., Freed J.H., Tarentino A.L., Plummer T.H. Jr., Hart G.W.
    J. Biol. Chem. 260:4046-4054(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION AT ASN-105 AND ASN-145.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Heart, Kidney, Lung and Spleen.
  7. "Crystal structure of I-Ak in complex with a dominant epitope of lysozyme."
    Fremont D.H., Monnaie D., Nelson C.A., Hendrickson W.A., Unanue E.R.
    Immunity 8:305-317(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 27-208.

Entry informationi

Entry nameiHA2K_MOUSE
AccessioniPrimary (citable) accession number: P01910
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: October 1, 1996
Last modified: May 11, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.