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P01901 (HA1B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 140. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
H-2 class I histocompatibility antigen, K-B alpha chain

Short name=H-2K(B)
Gene names
Name:H2-K1
Synonyms:H2-K
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length369 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the presentation of foreign antigens to the immune system.

Subunit structure

Heterodimer of an alpha chain and a beta chain (beta-2-microglobulin).

Subcellular location

Membrane; Single-pass type I membrane protein.

Sequence similarities

Belongs to the MHC class I family.

Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

Ontologies

Keywords
   Biological processImmunity
   Cellular componentMembrane
MHC I
   DomainSignal
Transmembrane
Transmembrane helix
   PTMDisulfide bond
Glycoprotein
Phosphoprotein
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processantigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent

Inferred from direct assay PubMed 1538753. Source: MGI

antigen processing and presentation of exogenous peptide antigen via MHC class I

Inferred from direct assay PubMed 16461341. Source: MGI

defense response to bacterium

Inferred from direct assay PubMed 16461341. Source: MGI

immune response

Inferred from electronic annotation. Source: InterPro

inner ear development

Inferred from direct assay PubMed 21795542. Source: MGI

positive regulation of T cell mediated cytotoxicity

Inferred from direct assay PubMed 1538753PubMed 16461341. Source: MGI

   Cellular_componentMHC class I protein complex

Inferred from electronic annotation. Source: UniProtKB-KW

external side of plasma membrane

Inferred from direct assay PubMed 11754812PubMed 12902470PubMed 14990792PubMed 15314074PubMed 1538753PubMed 9133426. Source: MGI

integral component of lumenal side of endoplasmic reticulum membrane

Traceable author statement. Source: Reactome

phagocytic vesicle membrane

Traceable author statement. Source: Reactome

   Molecular_functionpeptide antigen binding

Inferred from direct assay PubMed 16461341. Source: MGI

receptor binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

FesP168793EBI-1265227,EBI-771815

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Ref.4
Chain22 – 369348H-2 class I histocompatibility antigen, K-B alpha chain Ref.4
PRO_0000018928

Regions

Topological domain22 – 305284Extracellular Potential
Transmembrane306 – 32823Helical; Potential
Topological domain329 – 36941Cytoplasmic Potential
Domain206 – 29489Ig-like C1-type
Region22 – 11190Alpha-1
Region112 – 20392Alpha-2
Region204 – 29592Alpha-3
Region296 – 30510Connecting peptide

Amino acid modifications

Modified residue3541Phosphoserine Ref.5
Glycosylation1071N-linked (GlcNAc...) Ref.4 Ref.6
Glycosylation1971N-linked (GlcNAc...) Ref.4
Disulfide bond122 ↔ 185
Disulfide bond224 ↔ 280

Experimental info

Sequence conflict2171E → D AA sequence Ref.4
Sequence conflict2891E → Q AA sequence Ref.4
Sequence conflict3341N → A AA sequence Ref.4
Sequence conflict3641D → P AA sequence Ref.4

Secondary structure

.......................................................... 369
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P01901 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 3D2F125318193443

FASTA36941,302
        10         20         30         40         50         60 
MVPCTLLLLL AAALAPTQTR AGPHSLRYFV TAVSRPGLGE PRYMEVGYVD DTEFVRFDSD 

        70         80         90        100        110        120 
AENPRYEPRA RWMEQEGPEY WERETQKAKG NEQSFRVDLR TLLGYYNQSK GGSHTIQVIS 

       130        140        150        160        170        180 
GCEVGSDGRL LRGYQQYAYD GCDYIALNED LKTWTAADMA ALITKHKWEQ AGEAERLRAY 

       190        200        210        220        230        240 
LEGTCVEWLR RYLKNGNATL LRTDSPKAHV THHSRPEDKV TLRCWALGFY PADITLTWQL 

       250        260        270        280        290        300 
NGEELIQDME LVETRPAGDG TFQKWASVVV PLGKEQYYTC HVYHQGLPEP LTLRWEPPPS 

       310        320        330        340        350        360 
TVSNMATVAV LVVLGAAIVT GAVVAFVMKM RRRNTGGKGG DYALAPGSQT SDLSLPDCKV 


MVHDPHSLA 

« Hide

References

« Hide 'large scale' references
[1]"The DNA sequence of the H-2K(b) gene: evidence for gene conversion as a mechanism for the generation of polymorphism in histocompatibility antigens."
Weiss E., Golden L., Zakut R., Mellor A., Fahrner K., Kvist S., Flavell R.A.
EMBO J. 2:453-462(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[2]"Nucleotide sequences of three H-2K and three H-2D complementary DNA clones coding mouse class I MHC heavy chain proteins."
Wang M., Stepkowski S.M., Hebert J.S., Tian L., Yu J., Kahan B.D.
Ann. Transplant. 1:26-31(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/10.
[3]"Isolation of a cDNA clone for the murine transplantation antigen H-2Kb."
Reyes A.A., Schold M., Itakura K., Wallace R.B.
Proc. Natl. Acad. Sci. U.S.A. 79:3270-3274(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 87-369.
[4]"Amino acid sequence of the carboxyl-terminal hydrophilic region of the H-2Kb MHC alloantigen. Completion of the entire primary structure of the H-2Kb molecule."
Uehara H., Coligan J.E., Nathenson S.G.
Biochemistry 20:5940-5945(1981) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 22-367, GLYCOSYLATION AT ASN-107 AND ASN-197.
[5]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-354, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[6]"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-107.
[7]"Crystal structures of two viral peptides in complex with murine MHC class I H-2Kb."
Fremont D.H., Matsumura M., Stura E.A., Peterson P.A., Wilson I.A.
Science 257:919-927(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 22-295.
[8]"Structural basis of CD8 coreceptor function revealed by crystallographic analysis of a murine CD8alphaalpha ectodomain fragment in complex with H-2Kb."
Kern P.S., Teng M.K., Smolyar A., Liu J.H., Liu J., Hussey R.E., Spoerl R., Chang H.-C., Reinherz E.L., Wang J.-H.
Immunity 9:519-530(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 22-295 IN COMPLEX WITH CD8A.
[9]"Crystal structure of an MHC class I presented glycopeptide that generates carbohydrate-specific CTL."
Speir J.A., Abdel-Motal U.M., Jondal M., Wilson I.A.
Immunity 10:51-61(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 22-295.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J00400 mRNA. Translation: AAA39648.1.
U47328 mRNA. Translation: AAB17606.1.
V00746, V00747 Genomic DNA. Translation: CAA24119.2.
PIRHLMSKB. A90980.
RefSeqNP_001001892.2. NM_001001892.2.
UniGeneMm.422886.
Mm.439675.
Mm.440940.
Mm.466882.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1BQHX-ray2.80A/D22-295[»]
1FO0X-ray2.50H22-296[»]
1FZJX-ray1.90A22-295[»]
1FZKX-ray1.70A22-295[»]
1FZMX-ray1.80A22-295[»]
1FZOX-ray1.80A22-295[»]
1G6RX-ray2.80H/I22-295[»]
1G7PX-ray1.50A22-295[»]
1G7QX-ray1.60A22-295[»]
1KBGX-ray2.20H22-295[»]
1KJ2X-ray2.71H/I22-298[»]
1KJ3X-ray2.30H/I22-299[»]
1KPUX-ray1.50A22-295[»]
1KPVX-ray1.71A22-295[»]
1LEGX-ray1.75A22-295[»]
1LEKX-ray2.15A22-295[»]
1LK2X-ray1.35A22-295[»]
1MWAX-ray2.40H/I22-295[»]
1N59X-ray2.95A/C22-297[»]
1NAMX-ray2.70H22-296[»]
1NANX-ray2.30H/L22-299[»]
1OSZX-ray2.10A22-295[»]
1P1ZX-ray3.26A22-295[»]
1P4LX-ray2.90A22-295[»]
1RJYX-ray1.90A/D22-301[»]
1RJZX-ray2.60A/D22-301[»]
1RK0X-ray2.61A22-295[»]
1RK1X-ray2.10A22-295[»]
1S7QX-ray1.99A22-369[»]
1S7RX-ray2.95A/D22-369[»]
1S7SX-ray1.99A22-369[»]
1S7TX-ray2.30A/D22-369[»]
1T0MX-ray2.00A/D22-299[»]
1T0NX-ray1.80A/D22-299[»]
1VACX-ray2.50A22-295[»]
1VADX-ray2.50A22-295[»]
1WBZX-ray2.00A/C22-296[»]
2CKBX-ray3.00H/I22-295[»]
2CLVX-ray1.90A/H22-300[»]
2CLZX-ray1.90A/H22-300[»]
2FO4X-ray2.70A22-295[»]
2MHAX-ray2.50A/C22-291[»]
2OL3X-ray2.90H22-300[»]
2QRIX-ray2.00A/B22-301[»]
2QRSX-ray2.00A/B22-301[»]
2QRTX-ray1.80A/B22-301[»]
2VAAX-ray2.30A22-295[»]
2VABX-ray2.50A22-295[»]
2ZSVX-ray1.80A/C22-299[»]
2ZSWX-ray2.80A/C/E/G22-299[»]
3C8KX-ray2.90A22-295[»]
3CVHX-ray2.90A/M22-295[»]
3P4MX-ray2.50A/D22-298[»]
3P4NX-ray2.50A/D22-298[»]
3P4OX-ray2.30A/D22-298[»]
3P9LX-ray2.00A/D22-299[»]
3P9MX-ray2.00A/D22-298[»]
3PABX-ray2.20A/D22-299[»]
3RGVX-ray2.90C22-296[»]
3ROLX-ray2.60A/C22-296[»]
3ROOX-ray2.00A/C22-296[»]
3TIDX-ray1.65A22-297[»]
3TIEX-ray2.25A/D22-297[»]
4HKJX-ray3.00A/E/I/M22-301[»]
ProteinModelPortalP01901.
SMRP01901. Positions 22-295.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-6189N.
IntActP01901. 3 interactions.
MINTMINT-241080.

Proteomic databases

PaxDbP01901.
PRIDEP01901.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000025181; ENSMUSP00000025181; ENSMUSG00000061232.
GeneID14972.
KEGGmmu:14972.
UCSCuc008cap.1. mouse.

Organism-specific databases

CTD14972.
MGIMGI:95904. H2-K1.

Phylogenomic databases

eggNOGNOG42056.
HOVERGENHBG016709.
InParanoidP01901.
KOK06751.
OMASEEWHRY.
TreeFamTF336617.

Enzyme and pathway databases

ReactomeREACT_102124. Immune System.
REACT_98458. Immune System.

Gene expression databases

ArrayExpressP01901.
BgeeP01901.
CleanExMM_H2-K1.
GenevestigatorP01901.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
3.30.500.10. 1 hit.
InterProIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR011161. MHC_I-like_Ag-recog.
IPR011162. MHC_I/II-like_Ag-recog.
IPR027648. MHC_I_a.
IPR001039. MHC_I_a_a1/a2.
IPR010579. MHC_I_a_C.
[Graphical view]
PfamPF07654. C1-set. 1 hit.
PF00129. MHC_I. 1 hit.
PF06623. MHC_I_C. 1 hit.
[Graphical view]
PRINTSPR01638. MHCCLASSI.
SMARTSM00407. IGc1. 1 hit.
[Graphical view]
SUPFAMSSF54452. SSF54452. 1 hit.
PROSITEPS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSH2-K1. mouse.
EvolutionaryTraceP01901.
NextBio287336.
PROP01901.
SOURCESearch...

Entry information

Entry nameHA1B_MOUSE
AccessionPrimary (citable) accession number: P01901
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: March 19, 2014
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot