Reviewed, UniProtKB/Swiss-Prot P01877 (IGHA2_HUMAN)

Names and origin

Protein names

Recommended name:
    Ig alpha-2 chain C region

Gene names

Name:

IGHA2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system.
Subunit structure	Monomeric or polymeric.
Miscellaneous	The sequence of the A2m1 allotype is shown.
Sequence similarities	Contains 3 Ig-like (immunoglobulin-like) domains.

Ontologies

Keywords
Domain	Immunoglobulin C region Immunoglobulin domain Repeat
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	immune response Ref.5 Non-traceable author statement. Source: UniProtKB
Cellular component	membrane fraction Non-traceable author statement. Source: UniProtKB
Molecular function	antigen binding Ref.1 Traceable author statement. Source: UniProtKB protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CDK2AP1	O14519	1	EBI-1044357,EBI-1052532
NUAK1	O60285	1	EBI-1044357,EBI-1046789
PFTK1	O94921	1	EBI-1044357,EBI-1043945

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 340	›340	Ig alpha-2 chain C region		PRO_0000153567
Regions
	Domain	6 – 98	93	Ig-like 1
	Domain	112 – 207	96	Ig-like 2
	Domain	215 – 317	103	Ig-like 3
Amino acid modifications
	Glycosylation	47	1	N-linked (GlcNAc...) Ref.10
	Glycosylation	92	1	N-linked (GlcNAc...) Ref.10
	Glycosylation	131	1	N-linked (GlcNAc...) Ref.10 Ref.7 Ref.9
	Glycosylation	205	1	N-linked (GlcNAc...) Ref.10 Ref.7 Ref.6 Ref.8
	Glycosylation	327	1	N-linked (GlcNAc...) Ref.10 Ref.8
	Disulfide bond	26 ↔ 85		Probable
	Disulfide bond	101		Interchain (with light chain) Probable
	Disulfide bond	109		Interchain (with heavy chain) Probable
	Disulfide bond	110 ↔ 167		Probable
	Disulfide bond	134 ↔ 191		Probable
	Disulfide bond	169		Interchain (with heavy chain) Probable
	Disulfide bond	179		Interchain (with heavy chain of another subunit) Potential
	Disulfide bond	237 ↔ 300		Probable
	Disulfide bond	339		Interchain (with J chain) Probable
Natural variations
	Natural variant	93	1	P → S in A2M(2) allotype.		VAR_003879
	Natural variant	102	1	P → R in A2M(2) allotype.		VAR_003880
	Natural variant	279	1	F → Y in A2M(2) allotype.		VAR_003881
	Natural variant	296	1	D → E in A2M(2) allotype.		VAR_003882
	Natural variant	326	1	V → I in A2M(2) allotype.		VAR_003883
	Natural variant	335	1	V → A in A2M(2) allotype.		VAR_003884
Experimental info
	Sequence conflict	319	1	M → L in AAB59396. Ref.1
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P01877-1 [UniParc]. Last modified December 16, 2008. Version 3. Checksum: 98922700756EE906	FASTA	340	36,526
	10 20 30 40 50 60 ASPTSPKVFP LSLDSTPQDG NVVVACLVQG FFPQEPLSVT WSESGQNVTA RNFPPSQDAS 70 80 90 100 110 120 GDLYTTSSQL TLPATQCPDG KSVTCHVKHY TNPSQDVTVP CPVPPPPPCC HPRLSLHRPA 130 140 150 160 170 180 LEDLLLGSEA NLTCTLTGLR DASGATFTWT PSSGKSAVQG PPERDLCGCY SVSSVLPGCA 190 200 210 220 230 240 QPWNHGETFT CTAAHPELKT PLTANITKSG NTFRPEVHLL PPPSEELALN ELVTLTCLAR 250 260 270 280 290 300 GFSPKDVLVR WLQGSQELPR EKYLTWASRQ EPSQGTTTFA VTSILRVAAE DWKKGDTFSC 310 320 330 340 MVGHEALPLA FTQKTIDRMA GKPTHVNVSV VMAEVDGTCY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mechanisms of divergence and convergence of the human immunoglobulin alpha 1 and alpha 2 constant region gene sequences." Flanagan J.G., Lefranc M.-P., Rabbitts T.H. Cell 36:681-688(1984) [PubMed: 6421489] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The DNA sequence and analysis of human chromosome 14." Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J. Nature 421:601-607(2003) [PubMed: 12508121] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Complete amino acid sequence of the alpha 2 heavy chain of a human IgA2 immunoglobulin of the A2m (2) allotype." Torano A., Putnam F.W. Proc. Natl. Acad. Sci. U.S.A. 75:966-969(1978) [PubMed: 416441] [Abstract] Cited for: PROTEIN SEQUENCE (BUT).
[4]	"Structure of the A2m(1) allotype of human IgA -- a recombinant molecule." Tsuzukida Y., Wang C.-C., Putnam F.W. Proc. Natl. Acad. Sci. U.S.A. 76:1104-1108(1979) [PubMed: 286295] [Abstract] Cited for: PROTEIN SEQUENCE (MYELOMA PROTEIN LAN).
[5]	"The structure and function of human IgA." Kerr M.A. Biochem. J. 271:285-296(1990) [PubMed: 2241915] [Abstract] Cited for: REVIEW.
[6]	"A proteomic analysis of human bile." Kristiansen T.Z., Bunkenborg J., Gronborg M., Molina H., Thuluvath P.J., Argani P., Goggins M.G., Maitra A., Pandey A. Mol. Cell. Proteomics 3:715-728(2004) [PubMed: 15084671] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-205, MASS SPECTROMETRY. Tissue: Bile.
[7]	"Screening for N-glycosylated proteins by liquid chromatography mass spectrometry." Bunkenborg J., Pilch B.J., Podtelejnikov A.V., Wisniewski J.R. Proteomics 4:454-465(2004) [PubMed: 14760718] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-131 AND ASN-205, MASS SPECTROMETRY. Tissue: Plasma.
[8]	"Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry." Ramachandran P., Boontheung P., Xie Y., Sondej M., Wong D.T., Loo J.A. J. Proteome Res. 5:1493-1503(2006) [PubMed: 16740002] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-205 AND ASN-327, MASS SPECTROMETRY. Tissue: Saliva.
[9]	"Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides." Bernhard O.K., Kapp E.A., Simpson R.J. J. Proteome Res. 6:987-995(2007) [PubMed: 17330941] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-131, MASS SPECTROMETRY. Tissue: Plasma.
[10]	"Identification of N-linked glycoproteins in human milk by hydrophilic interaction liquid chromatography and mass spectrometry." Picariello G., Ferranti P., Mamone G., Roepstorff P., Addeo F. Proteomics 8:3833-3847(2008) [PubMed: 18780401] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-47; ASN-92; ASN-131; ASN-205 AND ASN-327, MASS SPECTROMETRY. Tissue: Milk.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J00221 Genomic DNA. Translation: AAB59396.1. Different initiation. AL928742 Genomic DNA. No translation available.
IPI	IPI00641229.
PIR	B22360.
UniGene	Hs.510635
3D structure databases
HSSP	HSSP built from PDB template 1OW0 based on UniProtKB P01876.
SMR	P01877. Positions 110-318.
ModBase	Search...
Protein-protein interaction databases
IntAct	P01877. 3 interactions.
Protein family/group databases
IMGT	Search... Search...
Proteomic databases
PRIDE	P01877.
Genome annotation databases
Ensembl	ENSG00000211890. Homo sapiens. [Contig view]
Organism-specific databases
GeneCards	GC14M105124.
HGNC	HGNC:5479. IGHA2.
HPA	HPA001217. HPA006013.
MIM	147000. gene.
PharmGKB	PA29711.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	P01877.
Gene expression databases
ArrayExpress	P01877.
Bgee	P01877.
GermOnline	ENSG00000177199. Homo sapiens.
Family and domain databases
InterPro	IPR013151. Ig. IPR007110. Ig-like. IPR013783. Ig-like_fold. IPR003006. Ig/MHC_CS. IPR003597. Ig_C1-set. [Graphical view]
Gene3D	G3DSA:2.60.40.10. Ig-like_fold. 3 hits.
Pfam	PF07654. C1-set. 2 hits. PF00047. ig. 1 hit. [Graphical view]
SMART	SM00407. IGc1. 2 hits. [Graphical view]
PROSITE	PS50835. IG_LIKE. 3 hits. PS00290. IG_MHC. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
DrugBank	DB00028. Immune globulin.
SOURCE	Search...

Entry information

Entry name

IGHA2_HUMAN

Accession

Primary (citable) accession number: P01877

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	December 16, 2008
Last modified:	May 5, 2009
This is version 82 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)