P01867 (IGG2B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ig gamma-2B chain C region

Gene names

Name:

Igh-3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	404 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Isoform 1: Cell membrane; Single-pass membrane protein Potential. Isoform 2: Secreted.
Post-translational modification	O-linked glycan consists of Gal-GalNAc disaccharide which is modified with 2 sialic acid residues.
Polymorphism	The a allele sequence is shown.
Sequence similarities	Contains 3 Ig-like (immunoglobulin-like) domains.

Ontologies

Keywords
Cellular component	Cell membrane Membrane Secreted
Coding sequence diversity	Alternative splicing
Domain	Immunoglobulin C region Immunoglobulin domain Repeat Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	antigen binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P01867-1)

Also known as: Membrane-bound;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P01867-2)

Also known as: Secreted;

The sequence of this isoform differs from the canonical sequence as follows:
     335-335: L → K
     336-404: Missing.

Note: May be the major isoform.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

‹1 – 404

›404

Ig gamma-2B chain C region

PRO_0000153588

Regions

Transmembrane

351 – 368

18

Helical; Potential

Topological domain

369 – 404

36

Cytoplasmic Potential

Domain

5 – 97

93

Ig-like 1

Domain

126 – 225

100

Ig-like 2

Domain

234 – 330

97

Ig-like 3

Amino acid modifications

Glycosylation

104

1

O-linked (GalNAc...) Ref.7

Glycosylation

185

1

N-linked (GlcNAc...) Potential

Disulfide bond

14

Interchain (with a light chain) By similarity

Disulfide bond

26 ↔ 81

By similarity

Disulfide bond

108

Interchain (with a heavy chain) By similarity

Disulfide bond

111

Interchain (with a heavy chain) By similarity

Disulfide bond

114

Interchain (with a heavy chain) By similarity

Disulfide bond

117

Interchain (with a heavy chain) By similarity

Disulfide bond

149 ↔ 209

By similarity

Disulfide bond

255 ↔ 313

By similarity

Natural variations

Alternative sequence

335

1

L → K in isoform 2.

VSP_034614

Alternative sequence

336 – 404

69

Missing in isoform 2.

VSP_034615

Natural variant

162

1

Q → R in allele B.

Natural variant

193

1

T → A in allele B.

Natural variant

299

1

N → D in allele B.

Natural variant

300

1

M → I in allele B.

Experimental info

Sequence conflict

24

1

L → S Ref.2

Sequence conflict

24

1

L → S Ref.3

Sequence conflict

35

1

S → P Ref.2

Sequence conflict

35

1

S → P Ref.3

Sequence conflict

238

1

I → T Ref.2

Sequence conflict

238

1

I → T Ref.3

Non-terminal residue

1

1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

404

Helix Strand Turn

Details...

Beta strand

7 – 11

5

Beta strand

14 – 16

3

Beta strand

21 – 35

15

Beta strand

38 – 41

4

Turn

42 – 45

4

Beta strand

50 – 52

3

Beta strand

56 – 58

3

Beta strand

61 – 71

11

Turn

72 – 77

6

Beta strand

81 – 86

6

Helix

87 – 89

3

Beta strand

91 – 96

6

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Membrane-bound) [UniParc]. Last modified February 1, 2005. Version 3. Checksum: AE17A03221F6EC0C	FASTA	404	44,259
	10 20 30 40 50 60 KTTPPSVYPL APGCGDTTGS SVTLGCLVKG YFPESVTVTW NSGSLSSSVH TFPALLQSGL 70 80 90 100 110 120 YTMSSSVTVP SSTWPSQTVT CSVAHPASST TVDKKLEPSG PISTINPCPP CKECHKCPAP 130 140 150 160 170 180 NLEGGPSVFI FPPNIKDVLM ISLTPKVTCV VVDVSEDDPD VQISWFVNNV EVHTAQTQTH 190 200 210 220 230 240 REDYNSTIRV VSTLPIQHQD WMSGKEFKCK VNNKDLPSPI ERTISKIKGL VRAPQVYILP 250 260 270 280 290 300 PPAEQLSRKD VSLTCLVVGF NPGDISVEWT SNGHTEENYK DTAPVLDSDG SYFIYSKLNM 310 320 330 340 350 360 KTSKWEKTDS FSCNVRHEGL KNYYLKKTIS RSPGLDLDDI CAEAKDGELD GLWTTITIFI 370 380 390 400 SLFLLSVCYS ASVTLFKVKW IFSSVVELKQ KISPDYRNMI GQGA
	Isoform 2 (Secreted) [UniParc]. Checksum: 557C4C96ED38965A	FASTA	335	36,588
	10 20 30 40 50 60 KTTPPSVYPL APGCGDTTGS SVTLGCLVKG YFPESVTVTW NSGSLSSSVH TFPALLQSGL 70 80 90 100 110 120 YTMSSSVTVP SSTWPSQTVT CSVAHPASST TVDKKLEPSG PISTINPCPP CKECHKCPAP 130 140 150 160 170 180 NLEGGPSVFI FPPNIKDVLM ISLTPKVTCV VVDVSEDDPD VQISWFVNNV EVHTAQTQTH 190 200 210 220 230 240 REDYNSTIRV VSTLPIQHQD WMSGKEFKCK VNNKDLPSPI ERTISKIKGL VRAPQVYILP 250 260 270 280 290 300 PPAEQLSRKD VSLTCLVVGF NPGDISVEWT SNGHTEENYK DTAPVLDSDG SYFIYSKLNM 310 320 330 KTSKWEKTDS FSCNVRHEGL KNYYLKKTIS RSPGK

References

[1]	"Complete nucleotide sequence of immunoglobulin gamma2b chain gene cloned from newborn nouse DNA." Yamawaki-Kataoka Y., Kataoka T., Takahashi N., Obata M., Honjo T. Nature 283:786-789(1980) [PubMed: 6766534] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Structure of the constant and 3' untranslated regions of the murine gamma 2b heavy chain messenger RNA." Tucker P.W., Marcu K.B., Slightom J.L., Blattner F.R. Science 206:1299-1303(1979) [PubMed: 117548] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (MPC 11).
[3]	"Sequence of the cloned gene for the constant region of murine gamma 2b immunoglobulin heavy chain." Tucker P.W., Marcu K.B., Newell N., Richards J., Blattner F.R. Science 206:1303-1306(1979) [PubMed: 117549] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[4]	"Mouse immunoglobulin allotypes: post-duplication divergence of gamma 2a and gamma 2b chain genes." Ollo R., Rougeon F. Nature 296:761-763(1982) [PubMed: 6803173] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ALLELE B).
[5]	"Nucleotide sequences of gene segments encoding membrane domains of immunoglobulin gamma chains." Yamawaki-Kataoka Y., Nakai S., Miyata T., Honjo T. Proc. Natl. Acad. Sci. U.S.A. 79:2623-2627(1982) [PubMed: 6283537] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 334-402.
[6]	"Gene segments encoding transmembrane carboxyl termini of immunoglobulin gamma chains." Rogers J., Choi E., Souza L., Carter C., Word C.J., Kuehl M., Eisenberg D., Wall R. Cell 26:19-27(1981) [PubMed: 6799207] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 334-377.
[7]	"O-glycosylation in hinge region of mouse immunoglobulin G2b." Kim H., Yamaguchi Y., Masuda K., Matsunaga C., Yamamoto K., Irimura T., Takahashi N., Kato K., Arata Y. J. Biol. Chem. 269:12345-12350(1994) [PubMed: 7512967] [Abstract] Cited for: GLYCOSYLATION AT THR-104.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J00461, J00462 Genomic DNA. Translation: AAB59659.1.

IPI

IPI00606550.
IPI00830619.

PIR

G2MSBM. C02154.
G2MS11. S25057.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1CIC	X-ray	2.50	B	2-99	[»]
1ETZ	X-ray	2.60	B/H	1-101	[»]
1FJ1	X-ray	2.68	B/D	1-99	[»]
1FL3	X-ray	2.45	A/H	1-97	[»]
1HQ4	X-ray	2.70	B/D	1-98	[»]
1MAM	X-ray	2.45	H	1-97	[»]
1NBV	X-ray	2.00	H	1-97	[»]
1OSP	X-ray	1.95	H	1-97	[»]
1UB5	X-ray	2.00	A/H	1-97	[»]
1UB6	X-ray	2.12	A/H	1-97	[»]
1YNK	X-ray	2.10	H	1-102	[»]
1YNL	X-ray	1.70	H	1-102	[»]
1ZTX	X-ray	2.50	H	1-99	[»]
2GJZ	X-ray	2.65	B/H	1-98	[»]
2GK0	X-ray	2.45	B/H	1-98	[»]
2Q8A	X-ray	2.40	H	1-97	[»]
2Q8B	X-ray	2.30	H	1-97	[»]
2RGS	X-ray	2.13	A/B	117-334	[»]
3CFD	X-ray	2.50	B/H	1-99	[»]
3CFE	X-ray	2.99	B/H	1-99	[»]

ProteinModelPortal

P01867.

SMR

P01867. Positions 1-331.

ModBase

Search...

Protein-protein interaction databases

MINT

MINT-1504211.

STRING

P01867.

Protein family/group databases

IMGT

Search...
Search...

Proteomic databases

PRIDE

P01867.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Organism-specific databases

MGI

MGI:96445. Igh-3.

Phylogenomic databases

HOGENOM

HBG505540.

HOVERGEN

HBG005814.

InParanoid

P01867.

OrthoDB

EOG4RXZ0H.

Gene expression databases

ArrayExpress

P01867.

Bgee

P01867.

Genevestigator

P01867.

Family and domain databases

InterPro

IPR007110. Ig-like.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
[Graphical view]

Gene3D

G3DSA:2.60.40.10. Ig-like_fold. 3 hits.

Pfam

PF07654. C1-set. 3 hits.
[Graphical view]

SMART

SM00407. IGc1. 2 hits.
[Graphical view]

PROSITE

PS50835. IG_LIKE. 3 hits.
PS00290. IG_MHC. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

SOURCE

Search...

Entry information

Entry name

IGG2B_MOUSE

Accession

Primary (citable) accession number: P01867
Secondary accession number(s): P01866

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	February 1, 2005
Last modified:	September 21, 2011
This is version 97 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program