P01865 (GCAM_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ig gamma-2A chain C region, membrane-bound form

Gene names

Name:

Igh-1a

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Cell membrane; Single-pass membrane protein Potential.
Sequence similarities	Contains 3 Ig-like (immunoglobulin-like) domains.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Immunoglobulin C region Immunoglobulin domain Repeat Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	antigen binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Membrane-bound (identifier: P01865-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Secreted (identifier: P01864-1)

The sequence of this isoform can be found in the external entry P01864.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Probably the major isoform.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

‹1 – 398

›398

Ig gamma-2A chain C region, membrane-bound form

PRO_0000153586

Regions

Transmembrane

345 – 362

18

Helical; Potential

Topological domain

363 – 398

36

Cytoplasmic Potential

Domain

5 – 97

93

Ig-like 1

Domain

120 – 219

100

Ig-like 2

Domain

228 – 324

97

Ig-like 3

Amino acid modifications

Glycosylation

179

1

N-linked (GlcNAc...) Ref.4

Disulfide bond

14

Interchain (with a light chain)

Disulfide bond

26 ↔ 81

Disulfide bond

106

Interchain (with a heavy chain)

Disulfide bond

109

Interchain (with a heavy chain)

Disulfide bond

111

Interchain (with a heavy chain)

Disulfide bond

143 ↔ 203

Disulfide bond

249 ↔ 307

Experimental info

Non-terminal residue

1

1

Secondary structure

1

.

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398

Helix Strand Turn

Details...

Beta strand

6 – 10

5

Helix

13 – 15

3

Beta strand

19 – 34

16

Beta strand

37 – 40

4

Helix

41 – 43

3

Beta strand

49 – 51

3

Beta strand

55 – 57

3

Beta strand

60 – 70

11

Turn

71 – 76

6

Beta strand

80 – 85

6

Helix

86 – 88

3

Beta strand

90 – 95

6

Sequences

	Sequence		Length	Mass (Da)
	Isoform Membrane-bound [UniParc]. Last modified February 1, 2005. Version 3. Checksum: B9CE2EE11224D13B	FASTA	398	43,949
	10 20 30 40 50 60 KTTAPSVYPL APVCGDTTGS SVTLGCLVKG YFPEPVTLTW NSGSLSSGVH TFPAVLQSDL 70 80 90 100 110 120 YTLSSSVTVT SSTWPSQSIT CNVAHPASST KVDKKIEPRG PTIKPCPPCK CPAPNLLGGP 130 140 150 160 170 180 SVFIFPPKIK DVLMISLSPI VTCVVVDVSE DDPDVQISWF VNNVEVHTAQ TQTHREDYNS 190 200 210 220 230 240 TLRVVSALPI QHQDWMSGKE FKCKVNNKDL PAPIERTISK PKGSVRAPQV YVLPPPEEEM 250 260 270 280 290 300 TKKQVTLTCM VTDFMPEDIY VEWTNNGKTE LNYKNTEPVL DSDGSYFMYS KLRVEKKNWV 310 320 330 340 350 360 ERNSYSCSVV HEGLHNHHTT KSFSRTPGLD LDDVCAEAQD GELDGLWTTI TIFISLFLLS 370 380 390 VCYSASVTLF KVKWIFSSVV ELKQTISPDY RNMIGQGA
	Isoform Secreted [UniParc]. See P01864.		–	–

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete nucleotide sequence of mouse immunoglobin gamma 2a gene and evolution of heavy chain genes: further evidence for intervening sequence-mediated domain transfer." Yamawaki-Kataoka Y., Miyata T., Honjo T. Nucleic Acids Res. 9:1365-1381(1981) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Nucleotide sequences of gene segments encoding membrane domains of immunoglobulin gamma chains." Yamawaki-Kataoka Y., Nakai S., Miyata T., Honjo T. Proc. Natl. Acad. Sci. U.S.A. 79:2623-2627(1982) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 329-398.
[3]	"Sequence and polyadenylation site determination of the murine immunoglobulin gamma 2a membrane 3' untranslated region." Hall B., Milcarek C. Mol. Immunol. 26:819-826(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 372-398.
[4]	"Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides." Bernhard O.K., Kapp E.A., Simpson R.J. J. Proteome Res. 6:987-995(2007) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-179, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Plasma.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J00470, J00471 Genomic DNA. Translation: AAB59661.1.
M35032 Genomic DNA. Translation: AAA37919.1.

IPI

IPI00968965.

PIR

G2MSAM. A02154.

UniGene

Mm.342177.
Mm.436014.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1AXT	X-ray	2.15	H	1-99	[»]
1EGJ	X-ray	2.80	H	1-98	[»]
1FLR	X-ray	1.85	H	1-100	[»]
1FPT	X-ray	3.00	H	1-99	[»]
1GGB	X-ray	2.80	H	1-99	[»]
1GGC	X-ray	2.80	H	1-99	[»]
1KB5	X-ray	2.50	H	1-99	[»]
1LO0	X-ray	2.00	H/Y	1-101	[»]
1NBY	X-ray	1.80	B	1-96	[»]
1NCA	X-ray	2.50	H	1-100	[»]
1NCB	X-ray	2.50	H	1-100	[»]
1NCC	X-ray	2.50	H	1-100	[»]
1NCD	X-ray	2.90	H	1-100	[»]
1ND0	X-ray	2.45	B/D/F/H	1-102	[»]
1NDG	X-ray	1.90	B	1-96	[»]
1NDM	X-ray	2.10	B	1-96	[»]
1ORQ	X-ray	3.20	B	1-99	[»]
1PLG	X-ray	2.80	H	1-97	[»]
1RU9	X-ray	2.50	H	1-102	[»]
1RUA	X-ray	1.75	H	1-102	[»]
1RUK	X-ray	1.40	H	1-102	[»]
1RUL	X-ray	1.88	H	1-102	[»]
1RUM	X-ray	1.48	H	1-102	[»]
1RUP	X-ray	1.40	H	1-102	[»]
1YEE	X-ray	2.20	H	1-98	[»]
4FAB	X-ray	2.70	H	1-97	[»]

ProteinModelPortal

P01865.

SMR

P01865. Positions 1-325.

ModBase

Search...

Protein-protein interaction databases

IntAct

P01865. 2 interactions.

MINT

MINT-132839.

Protein family/group databases

IMGT

Search...
Search...
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Proteomic databases

PRIDE

P01865.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Organism-specific databases

MGI

MGI:96443. Igh-1a.

Phylogenomic databases

HOVERGEN

HBG005814.

Gene expression databases

Genevestigator

P01865.

Family and domain databases

Gene3D

2.60.40.10. 3 hits.

InterPro

IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
[Graphical view]

Pfam

PF07654. C1-set. 3 hits.
[Graphical view]

SMART

SM00407. IGc1. 2 hits.
[Graphical view]

PROSITE

PS50835. IG_LIKE. 3 hits.
PS00290. IG_MHC. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

DrugBank

DB00078. Ibritumomab.

EvolutionaryTrace

P01865.

SOURCE

Search...

Entry information

Entry name

GCAM_MOUSE

Accession

Primary (citable) accession number: P01865

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	February 1, 2005
Last modified:	May 29, 2013
This is version 112 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program