Reviewed, UniProtKB/Swiss-Prot P01864 (GCAB_MOUSE)

Names and origin

Protein names	Recommended name:     Ig gamma-2A chain C region secreted form Alternative name(s):     B allele
Organism	Mus musculus (Mouse)
Taxonomic identifier	10090 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subcellular location	Isoform Secreted: Secreted Potential.
Miscellaneous	The sequence differs from that of the a allele, from BALB/c mice, at 15% of the positions.
Sequence similarities	Contains 3 Ig-like (immunoglobulin-like) domains.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Immunoglobulin C region Immunoglobulin domain Repeat
PTM	Glycoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	antigen binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Secreted (identifier: P01864-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Probably the major isoform.

Isoform Membrane-bound (identifier: P01865-1)

The sequence of this isoform can be found in the external entry P01865-1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

‹1 – 335

›335

Ig gamma-2A chain C region secreted form

PRO_0000153585

Regions

Domain

6 – 98

93

Ig-like 1

Domain

126 – 225

100

Ig-like 2

Domain

234 – 330

97

Ig-like 3

Amino acid modifications

Glycosylation

185

1

N-linked (GlcNAc...) Ref.3

Experimental info

Non-terminal residue

1

1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

335

Helix Strand Turn

Details...

Beta strand

7 – 11

5

Beta strand

21 – 35

15

Beta strand

38 – 41

4

Turn

42 – 45

4

Beta strand

50 – 52

3

Beta strand

56 – 58

3

Beta strand

61 – 71

11

Turn

72 – 77

6

Beta strand

81 – 86

6

Helix

87 – 89

3

Beta strand

91 – 96

6

Sequences

	Sequence		Length	Mass (Da)
	Isoform Secreted [UniParc]. Last modified July 21, 1986. Version 1. Checksum: FA3382792CBB13C6	FASTA	335	36,596
	10 20 30 40 50 60 AKTTAPSVYP LVPVCGGTTG SSVTLGCLVK GYFPEPVTLT WNSGSLSSGV HTFPALLQSG 70 80 90 100 110 120 LYTLSSSVTV TSNTWPSQTI TCNVAHPASS TKVDKKIEPR VPITQNPCPP HQRVPPCAAP 130 140 150 160 170 180 DLLGGPSVFI FPPKIKDVLM ISLSPMVTCV VVDVSEDDPD VQISWFVNNV EVHTAQTQTH 190 200 210 220 230 240 REDYNSTLRV VSALPIQHQD WMSGKEFKCK VNNRALPSPI EKTISKPRGP VRAPQVYVLP 250 260 270 280 290 300 PPAEEMTKKE FSLTCMITGF LPAEIAVDWT SNGRTEQNYK NTATVLDSDG SYFMYSKLRV 310 320 330 QKSTWERGSL FACSVVHEVL HNHLTTKTIS RSLGK
	Isoform Membrane-bound. See P01865.	FASTA	–	–

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Multiple differences between the nucleic acid sequences of the IgG2aa and IgG2ab alleles of the mouse." Schreier P.H., Bothwell A.L.M., Mueller-Hill B., Baltimore D. Proc. Natl. Acad. Sci. U.S.A. 78:4495-4499(1981) [PubMed: 6170065] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C57BL/6.
[2]	"Multiple amino acid substitutions between murine gamma 2a heavy chain Fc regions of Ig1a and Ig1b allotypic forms." Dognin M.J., Lauwereys M., Strosberg A.D. Proc. Natl. Acad. Sci. U.S.A. 78:4031-4035(1981) [PubMed: 6794027] [Abstract] Cited for: PROTEIN SEQUENCE.
[3]	"Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides." Bernhard O.K., Kapp E.A., Simpson R.J. J. Proteome Res. 6:987-995(2007) [PubMed: 17330941] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-185, MASS SPECTROMETRY. Tissue: Plasma.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

J00479 Genomic DNA. No translation available.

IPI

IPI00831380.

PIR

G2MSAB. A02153.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BOG	X-ray	2.60	B	1-100	[»]
1CFN	X-ray	2.65	B	1-101	[»]
1CFQ	X-ray	2.80	B	1-101	[»]
1CFS	X-ray	2.75	B	1-101	[»]
1CFT	X-ray	2.80	B	1-101	[»]
1HH6	X-ray	2.60	B	1-101	[»]
1HH9	X-ray	2.70	B	1-101	[»]
1HI6	X-ray	2.55	B	1-101	[»]
2IPT	X-ray	2.00	H	1-100	[»]
2IQ9	X-ray	2.30	H	1-100	[»]
2IQA	X-ray	2.00	B/H	1-100	[»]
2VWE	X-ray	3.40	E/L	1-99	[»]

SMR

P01864. Positions 1-331.

ModBase

Search...

Protein-protein interaction databases

STRING

P01864.

Protein family/group databases

IMGT

Search...

Proteomic databases

PRIDE

P01864.

Genome annotation databases

Ensembl

ENSMUST00000103416; ENSMUSP00000100212; ENSMUSG00000076612; Mus musculus. [Genome view]

UCSC

uc007pgl.1. mouse.

Phylogenomic databases

HOVERGEN

P01864.

Gene expression databases

ArrayExpress

P01864.

Bgee

P01864.

Genevestigator

P01864.

Family and domain databases

InterPro

IPR007110. Ig-like.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
[Graphical view]

Gene3D

G3DSA:2.60.40.10. Ig-like_fold. 3 hits.

Pfam

PF07654. C1-set. 3 hits.
[Graphical view]

SMART

SM00407. IGc1. 2 hits.
[Graphical view]

PROSITE

PS50835. IG_LIKE. 3 hits.
PS00290. IG_MHC. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

GCAB_MOUSE

Accession

Primary (citable) accession number: P01864

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	November 3, 2009
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)