Reviewed, UniProtKB/Swiss-Prot P01843 (LAC1_MOUSE)

Names and origin

Protein names	Recommended name:     Ig lambda-1 chain C region
Organism	Mus musculus (Mouse)
Taxonomic identifier	10090 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	105 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Miscellaneous	The MOPC 315 cell line produces 2 light chains, 1 normal lambda-2 chain and 1 abnormal lambda-1 chain that is missing a large part of the V region. The C region sequence (shown here) appears completely normal.
Sequence similarities	Contains 1 Ig-like (immunoglobulin-like) domain.

Ontologies

Keywords
Domain	Immunoglobulin C region Immunoglobulin domain
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Cellular component	B cell receptor complex Inferred from direct assay. Source: MGI external side of plasma membrane Inferred from direct assay. Source: MGI
Molecular function	antigen binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

‹1 – 105

›105

Ig lambda-1 chain C region

PRO_0000153608

Regions

Domain

6 – 100

95

Ig-like

Amino acid modifications

Disulfide bond

27 ↔ 86

Disulfide bond

104

Interchain (with heavy chain)

Experimental info

Sequence conflict

19 – 20

2

ET → TE AA sequence Ref.4

Sequence conflict

56

1

Q → E AA sequence Ref.4

Sequence conflict

75

1

Missing AA sequence Ref.4

Sequence conflict

81 – 82

2

HS → SH AA sequence Ref.4

Sequence conflict

85

1

S → SS AA sequence Ref.4

Sequence conflict

96

1

E → Q AA sequence Ref.4

Non-terminal residue

1

1

Secondary structure

1

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105

Helix Strand Turn

Details...

Beta strand

7 – 11

5

Helix

15 – 18

4

Turn

19 – 21

3

Beta strand

22 – 35

14

Beta strand

38 – 43

6

Beta strand

46 – 48

3

Beta strand

52 – 54

3

Beta strand

58 – 60

3

Turn

61 – 63

3

Beta strand

64 – 74

11

Helix

75 – 80

6

Beta strand

84 – 90

7

Beta strand

93 – 99

7

Sequences

	Sequence		Length	Mass (Da)
	P01843-1 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: A89F2B09BCFCA018	FASTA	105	11,575
	10 20 30 40 50 60 QPKSSPSVTL FPPSSEELET NKATLVCTIT DFYPGVVTVD WKVDGTPVTQ GMETTQPSKQ 70 80 90 100 SNNKYMASSY LTLTARAWER HSSYSCQVTH EGHTVEKSLS RADCS

References

[1]	"Evolution of mouse immunoglobulin lambda genes." Selsing E., Miller J., Wilson R., Storb U. Proc. Natl. Acad. Sci. U.S.A. 79:4681-4685(1982) [PubMed: 6812053] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Dual expression of lambda genes in the MOPC-315 plasmacytoma." Bothwell A.L.M., Paskind M., Schwartz R.C., Sonenshein G.E., Gefter M.L., Baltimore D. Nature 290:65-67(1981) [PubMed: 6259534] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (MOPC 315).
[3]	"Somatic variants of murine immunoglobulin lambda light chains." Bothwell A.L.M., Paskind M., Reth M., Imanishi-Kari T., Rajewsky K., Baltimore D. Nature 298:380-382(1982) [PubMed: 6283385] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (S43).
[4]	"Amino acid sequences of two mouse immunoglobulin lambda chains." Appella E. Proc. Natl. Acad. Sci. U.S.A. 68:590-594(1971) [PubMed: 5276767] [Abstract] Cited for: PROTEIN SEQUENCE (MYELOMA PROTEIN MOPC 104E).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

J00582 mRNA. Translation: AAA51636.1.
J00587 Genomic DNA. Translation: AAB59672.1.

IPI

IPI00673369.

PIR

L1MS. A93922.

UniGene

Mm.326349

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1JN6	X-ray	2.70	A	1-100	[»]
1JNH	X-ray	2.85	A/C/E/G	1-100	[»]

ModBase

Search...

Protein family/group databases

IMGT

Search...
Search...

Proteomic databases

PRIDE

P01843.

Genome annotation databases

Ensembl

ENSMUSG00000076929. Mus musculus. [Contig view]

Phylogenomic databases

HOVERGEN

P01843.

OMA

P01843. ETTKPLK.

Gene expression databases

Bgee

P01843.

GermOnline

ENSMUSG00000061944. Mus musculus.

Family and domain databases

InterPro

IPR007110. Ig-like.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
[Graphical view]

Gene3D

G3DSA:2.60.40.10. Ig-like_fold. 1 hit.

Pfam

PF07654. C1-set. 1 hit.
[Graphical view]

SMART

SM00407. IGc1. 1 hit.
[Graphical view]

PROSITE

PS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

BindingDB

P01843.

Entry information

Entry name

LAC1_MOUSE

Accession

Primary (citable) accession number: P01843

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	June 16, 2009
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)