P01822 (HVM46_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Ig heavy chain V region MOPC 315
Organism	Mus musculus (Mouse)
Taxonomic identifier	10090 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	137 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Miscellaneous

This alpha chain was isolated from a myeloma protein that has anti-dinitrophenyl activity.

Ontologies

Keywords
Domain	Immunoglobulin V region Immunoglobulin domain Signal
PTM	Disulfide bond
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Molecular function	antigen binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Ref.2 Ref.4
	Chain	19 – 137	119	Ig heavy chain V region MOPC 315		PRO_0000015232
Regions
	Region	19 – 48	30	Framework-1
	Region	49 – 54	6	Complementarity-determining-1
	Region	55 – 68	14	Framework-2
	Region	69 – 84	16	Complementarity-determining-2
	Region	85 – 116	32	Framework-3
	Region	117 – 126	10	Complementarity-determining-3
	Region	127 – 137	11	Framework-4
Amino acid modifications
	Disulfide bond	40 ↔ 114		By similarity
Experimental info
	Sequence conflict	15	1	G → GG in CAA30727. Ref.1
	Sequence conflict	15	1	G → H AA sequence Ref.2
	Sequence conflict	77 – 78	2	GY → YG AA sequence Ref.4
	Sequence conflict	102	1	N → D AA sequence Ref.4
	Sequence conflict	123	1	Missing AA sequence Ref.4
	Non-terminal residue	137	1

Sequences

	Sequence		Length	Mass (Da)
	P01822 [UniParc]. Last modified August 1, 1992. Version 2. Checksum: FB3828304C2B81DC	FASTA	137	15,399
	10 20 30 40 50 60 MKVLSLLYLL TAIPGIMSDV QLQESGPGLV KPSQSLSLTC SVTGYSITSG YFWNWIRQFP 70 80 90 100 110 120 GNKLEWLGFI KYDGSNGYNP SLKNRVSITR DTSENQFFLK LNSVTTEDTA TYYCAGDNDH 130 LYYFDYWGQG TTLTVSS

References

[1]	"Cloning, sequencing and expression of the rearranged MOPC 315 VH gene segment." Rinfret A., Horne C., Dorrington K.J., Klein M. Mol. Immunol. 26:431-434(1989) [PubMed: 2497341] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Amino acid sequence of the precursor region of MOPC-315 mouse immunoglobulin heavy chain." Jilka R.L., Pestka S. Proc. Natl. Acad. Sci. U.S.A. 74:5692-5696(1977) [PubMed: 414225] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-31.
[3]	"Structure and function of immunoglobulin genes and precursors." Schechter I., Wolf O., Zemell R., Burstein Y. Fed. Proc. 38:1839-1845(1979) [PubMed: 428562] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-21.
[4]	"Amino-acid sequence of the variable region of the heavy (alpha) chain of a mouse myeloma protein with anti-hapten activity." Francis S.H., Leslie R.G.Q., Hood L., Eisen H.N. Proc. Natl. Acad. Sci. U.S.A. 71:1123-1127(1974) [PubMed: 4524622] [Abstract] Cited for: PROTEIN SEQUENCE OF 19-136.
[5]	"Model-building studies of antigen-binding sites: the hapten-binding site of mopc-315." Padlan E.A., Davies D.R., Pecht I., Givol D., Wright C. Cold Spring Harb. Symp. Quant. Biol. 41:627-637(1977) [PubMed: 268248] [Abstract] Cited for: SEQUENCE REVISION TO 53.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M27638 Genomic DNA. Translation: AAA61337.1. X07880 Genomic DNA. Translation: CAA30727.1.
IPI	IPI00457481.
PIR	AVMS35. PL0102.
UniGene	Mm.303767.
3D structure databases
ProteinModelPortal	P01822.
SMR	P01822. Positions 19-137.
ModBase	Search...
Protein-protein interaction databases
STRING	P01822.
Protein family/group databases
IMGT	Search... Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	roNOG17128.
HOGENOM	HBG716786.
HOVERGEN	HBG018013.
InParanoid	P01822.
OrthoDB	EOG4R23WF.
Gene expression databases
Genevestigator	P01822.
Family and domain databases
InterPro	IPR007110. Ig-like. IPR013783. Ig-like_fold. IPR013106. Ig_V-set. IPR003596. Ig_V-set_subgr. [Graphical view]
Gene3D	G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF07686. V-set. 1 hit. [Graphical view]
SMART	SM00406. IGv. 1 hit. [Graphical view]
PROSITE	PS50835. IG_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HVM46_MOUSE

Accession

Primary (citable) accession number: P01822

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	August 1, 1992
Last modified:	September 21, 2011
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program