Reviewed, UniProtKB/Swiss-Prot P01751 (HVM07_MOUSE)

Names and origin

Protein names	Recommended name:     Ig heavy chain V region B1-8/186-2
Organism	Mus musculus (Mouse)
Taxonomic identifier	10090 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	139 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Miscellaneous

The B1-8 mu chain mRNA was cloned from a hybridoma making antibodies to the hapten (4-hydroxy-3-nitrophenyl)acetyl (NPb antibodies).

Ontologies

Keywords
Domain	Immunoglobulin V region Immunoglobulin domain Signal
PTM	Disulfide bond
Technical term	3D-structure
Gene Ontology (GO)
Molecular function	antigen binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 19

19

Chain

20 – 139

120

Ig heavy chain V region B1-8/186-2

PRO_0000015220

Regions

Region

20 – 49

30

Framework-1

Region

50 – 54

5

Complementarity-determining-1

Region

55 – 68

14

Framework-2

Region

69 – 85

17

Complementarity-determining-2

Region

86 – 117

32

Framework-3

Region

118 – 124

7

D segment

Region

125 – 139

15

JH2 segment

Amino acid modifications

Disulfide bond

41 ↔ 115

By similarity

Experimental info

Non-terminal residue

139

1

Secondary structure

1

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139

Helix Strand Turn

Details...

Beta strand

22 – 24

3

Beta strand

27 – 31

5

Beta strand

37 – 46

10

Helix

48 – 50

3

Beta strand

51 – 58

8

Beta strand

64 – 70

7

Turn

72 – 74

3

Beta strand

77 – 79

3

Helix

81 – 83

3

Turn

84 – 86

3

Beta strand

87 – 92

6

Turn

93 – 96

4

Beta strand

97 – 102

6

Helix

107 – 109

3

Beta strand

111 – 118

8

Turn

120 – 123

4

Beta strand

126 – 129

4

Beta strand

133 – 137

5

Sequences

	Sequence		Length	Mass (Da)
	P01751-1 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 1B57DD4FD0C9F465	FASTA	139	15,419
	10 20 30 40 50 60 MGWSCIMLFL AATATGVHSQ VQLQQPGAEL VKPGASVKLS CKASGYTFTS YWMHWVKQRP 70 80 90 100 110 120 GRGLEWIGRI DPNSGGTKYN EKFKSKATLT VDKPSSTAYM QLSSLTSEDS AVYYCARYDY 130 YGSSYFDYWG QGTTLTVSS

References

[1]	"Heavy chain variable region contribution to the NPb family of antibodies: somatic mutation evident in a gamma 2a variable region." Bothwell A.L.M., Paskind M., Reth M., Imanishi-Kari T., Rajewsky K., Baltimore D. Cell 24:625-637(1981) [PubMed: 6788376] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

J00529 mRNA. Translation: AAA38170.1.

IPI

IPI00457410.

PIR

MHMS18. A90809.

UniGene

Mm.304472

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1A6U	X-ray	2.10	H	20-139	[»]
1A6V	X-ray	1.80	H/I/J	20-139	[»]
1A6W	X-ray	2.00	H	20-139	[»]
1NGP	X-ray	2.40	H	20-139	[»]
1NGQ	X-ray	2.40	H	20-139	[»]
1NQB	X-ray	2.00	A/C	20-139	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:27638N.

Protein family/group databases

IMGT

Search...

Genome annotation databases

Ensembl

ENSMUSG00000076732. Mus musculus. [Contig view]

Phylogenomic databases

HOVERGEN

P01751.

Gene expression databases

Bgee

P01751.

GermOnline

ENSMUSG00000072795. Mus musculus.

Family and domain databases

InterPro

IPR007110. Ig-like.
IPR013783. Ig-like_fold.
IPR013106. Ig_V-set.
IPR003596. Ig_V-set_sub.
[Graphical view]

Gene3D

G3DSA:2.60.40.10. Ig-like_fold. 1 hit.

Pfam

PF07686. V-set. 1 hit.
[Graphical view]

SMART

SM00406. IGv. 1 hit.
[Graphical view]

PROSITE

PS50835. IG_LIKE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

BindingDB

P01751.

Entry information

Entry name

HVM07_MOUSE

Accession

Primary (citable) accession number: P01751
Secondary accession number(s): P01752

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	June 16, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)