ID   TVBL3_HUMAN             Reviewed;         115 AA.
AC   P01733; A0A0A6YYD5; A0A5A7;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   18-JUL-2018, sequence version 2.
DT   27-MAR-2024, entry version 155.
DE   RecName: Full=T cell receptor beta variable 12-3 {ECO:0000303|Ref.5};
DE   AltName: Full=T-cell receptor beta chain V region YT35 {ECO:0000305|PubMed:6336315};
DE   Flags: Precursor;
GN   Name=TRBV12-3 {ECO:0000303|Ref.5};
GN   Synonyms=TCRBV12S3 {ECO:0000303|PubMed:8650574}, TCRBV8S1
GN   {ECO:0000303|PubMed:8650574};
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=6336315; DOI=10.1038/308145a0;
RA   Yanagi Y., Yoshikai Y., Leggett K., Clark S.P., Aleksander I., Mak T.W.;
RT   "A human T cell-specific cDNA clone encodes a protein having extensive
RT   homology to immunoglobulin chains.";
RL   Nature 308:145-149(1984).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3866244; DOI=10.1073/pnas.82.24.8624;
RA   Toyonaga B., Yoshikai Y., Vadasz V., Chin B., Mak T.W.;
RT   "Organization and sequences of the diversity, joining, and constant region
RT   genes of the human T-cell receptor beta chain.";
RL   Proc. Natl. Acad. Sci. U.S.A. 82:8624-8628(1985).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] (IMGT ALLELE TRBV12-3*01).
RX   PubMed=8650574; DOI=10.1126/science.272.5269.1755;
RA   Rowen L., Koop B.F., Hood L.;
RT   "The complete 685-kilobase DNA sequence of the human beta T cell receptor
RT   locus.";
RL   Science 272:1755-1762(1996).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (IMGT ALLELE TRBV12-3*01).
RX   PubMed=12853948; DOI=10.1038/nature01782;
RA   Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H.,
RA   Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K.,
RA   Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A.,
RA   Delehaunty K.D., Miner T.L., Nash W.E., Cordes M., Du H., Sun H.,
RA   Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A.,
RA   Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P.,
RA   Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M.,
RA   Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S.,
RA   Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R.,
RA   Strowmatt C., Latreille P., Miller N., Johnson D., Murray J.,
RA   Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W.,
RA   Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E.,
RA   Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A.,
RA   Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E.,
RA   Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E.,
RA   Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A.,
RA   Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A.,
RA   Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R.,
RA   McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H.,
RA   Wilson R.K.;
RT   "The DNA sequence of human chromosome 7.";
RL   Nature 424:157-164(2003).
RN   [5]
RP   NOMENCLATURE.
RA   Lefranc M.P., Lefranc G.;
RT   "The T Cell Receptor FactsBook.";
RL   (In) Lefranc M.P., Lefranc G. (eds.);
RL   The T Cell Receptor FactsBook., pp.1-397, Academic Press, London. (2001).
RN   [6]
RP   REVIEW ON T CELL REPERTOIRE DIVERSITY.
RX   PubMed=15040585; DOI=10.1038/nri1292;
RA   Nikolich-Zugich J., Slifka M.K., Messaoudi I.;
RT   "The many important facets of T-cell repertoire diversity.";
RL   Nat. Rev. Immunol. 4:123-132(2004).
RN   [7]
RP   GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-90.
RC   TISSUE=Leukemic T-cell;
RX   PubMed=19349973; DOI=10.1038/nbt.1532;
RA   Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M.,
RA   Schiess R., Aebersold R., Watts J.D.;
RT   "Mass-spectrometric identification and relative quantification of N-linked
RT   cell surface glycoproteins.";
RL   Nat. Biotechnol. 27:378-386(2009).
RN   [8]
RP   REVIEW ON T CELL RECEPTOR-CD3 COMPLEX ASSEMBLY, AND SUBCELLULAR LOCATION.
RX   PubMed=20452950; DOI=10.1101/cshperspect.a005140;
RA   Wucherpfennig K.W., Gagnon E., Call M.J., Huseby E.S., Call M.E.;
RT   "Structural biology of the T-cell receptor: insights into receptor
RT   assembly, ligand recognition, and initiation of signaling.";
RL   Cold Spring Harb. Perspect. Biol. 2:A005140-A005140(2010).
RN   [9]
RP   REVIEW ON T CELL RECEPTOR SIGNALING.
RX   PubMed=23524462; DOI=10.1038/nri3403;
RA   Brownlie R.J., Zamoyska R.;
RT   "T cell receptor signalling networks: branched, diversified and bounded.";
RL   Nat. Rev. Immunol. 13:257-269(2013).
RN   [10]
RP   NOMENCLATURE.
RX   PubMed=24600447; DOI=10.3389/fimmu.2014.00022;
RA   Lefranc M.P.;
RT   "Immunoglobulin and T Cell Receptor Genes: IMGT((R)) and the Birth and Rise
RT   of Immunoinformatics.";
RL   Front. Immunol. 5:22-22(2014).
RN   [11]
RP   REVIEW ON FUNCTION.
RX   PubMed=25493333; DOI=10.1146/annurev-immunol-032414-112334;
RA   Rossjohn J., Gras S., Miles J.J., Turner S.J., Godfrey D.I., McCluskey J.;
RT   "T cell antigen receptor recognition of antigen-presenting molecules.";
RL   Annu. Rev. Immunol. 33:169-200(2015).
CC   -!- FUNCTION: V region of the variable domain of T cell receptor (TR) beta
CC       chain that participates in the antigen recognition (PubMed:24600447).
CC       Alpha-beta T cell receptors are antigen specific receptors which are
CC       essential to the immune response and are present on the cell surface of
CC       T lymphocytes. Recognize peptide-major histocompatibility (MH) (pMH)
CC       complexes that are displayed by antigen presenting cells (APC), a
CC       prerequisite for efficient T cell adaptive immunity against pathogens
CC       (PubMed:25493333). Binding of alpha-beta TR to pMH complex initiates
CC       TR-CD3 clustering on the cell surface and intracellular activation of
CC       LCK that phosphorylates the ITAM motifs of CD3G, CD3D, CD3E and CD247
CC       enabling the recruitment of ZAP70. In turn ZAP70 phosphorylates LAT,
CC       which recruits numerous signaling molecules to form the LAT
CC       signalosome. The LAT signalosome propagates signal branching to three
CC       major signaling pathways, the calcium, the mitogen-activated protein
CC       kinase (MAPK) kinase and the nuclear factor NF-kappa-B (NF-kB)
CC       pathways, leading to the mobilization of transcription factors that are
CC       critical for gene expression and essential for T cell growth and
CC       differentiation (PubMed:23524462). The T cell repertoire is generated
CC       in the thymus, by V-(D)-J rearrangement. This repertoire is then shaped
CC       by intrathymic selection events to generate a peripheral T cell pool of
CC       self-MH restricted, non-autoaggressive T cells. Post-thymic interaction
CC       of alpha-beta TR with the pMH complexes shapes TR structural and
CC       functional avidity (PubMed:15040585). {ECO:0000303|PubMed:15040585,
CC       ECO:0000303|PubMed:23524462, ECO:0000303|PubMed:24600447,
CC       ECO:0000303|PubMed:25493333}.
CC   -!- SUBUNIT: Alpha-beta TR is a heterodimer composed of an alpha and beta
CC       chain; disulfide-linked. The alpha-beta TR is associated with the
CC       transmembrane signaling CD3 coreceptor proteins to form the TR-CD3 (TcR
CC       or TCR). The assembly of alpha-beta TR heterodimers with CD3 occurs in
CC       the endoplasmic reticulum where a single alpha-beta TR heterodimer
CC       associates with one CD3D-CD3E heterodimer, one CD3G-CD3E heterodimer
CC       and one CD247 homodimer forming a stable octameric structure. CD3D-CD3E
CC       and CD3G-CD3E heterodimers preferentially associate with TR alpha and
CC       TR beta chains, respectively. The association of the CD247 homodimer is
CC       the last step of TcR assembly in the endoplasmic reticulum and is
CC       required for transport to the cell surface.
CC       {ECO:0000303|PubMed:20452950}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000303|PubMed:20452950}.
CC   -!- POLYMORPHISM: There are several alleles. The sequence shown is that of
CC       IMGT allele TRBV12-3*01. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA60669.1; Type=Miscellaneous discrepancy; Note=Sequence from TRBJ1-2 gene product.; Evidence={ECO:0000305};
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DR   EMBL; M14158; AAA60669.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; L36092; AAC13336.1; -; Genomic_DNA.
DR   EMBL; AC244196; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; A02000; RWHUVY.
DR   PIR; S03488; S03488.
DR   PIR; S03510; S03510.
DR   AlphaFoldDB; P01733; -.
DR   SMR; P01733; -.
DR   IntAct; P01733; 2.
DR   MINT; P01733; -.
DR   IMGT_GENE-DB; TRBV12-3; -.
DR   GlyCosmos; P01733; 1 site, No reported glycans.
DR   GlyGen; P01733; 1 site.
DR   iPTMnet; P01733; -.
DR   MetOSite; P01733; -.
DR   PhosphoSitePlus; P01733; -.
DR   BioMuta; ENSG00000274752; -.
DR   BioMuta; HGNC:12185; -.
DR   DMDM; 136492; -.
DR   MassIVE; P01733; -.
DR   PeptideAtlas; P01733; -.
DR   ProteomicsDB; 51454; -.
DR   ABCD; P01733; 1 sequenced antibody.
DR   Ensembl; ENST00000620569.1; ENSP00000477916.1; ENSG00000274752.1.
DR   Ensembl; ENST00000633292.1; ENSP00000487964.1; ENSG00000282208.1.
DR   AGR; HGNC:12185; -.
DR   GeneCards; TRBV12-3; -.
DR   HGNC; HGNC:12185; TRBV12-3.
DR   HPA; ENSG00000274752; Tissue enriched (lymphoid).
DR   neXtProt; NX_P01733; -.
DR   OpenTargets; ENSG00000274752; -.
DR   VEuPathDB; HostDB:ENSG00000274752; -.
DR   GeneTree; ENSGT00940000162707; -.
DR   HOGENOM; CLU_077975_9_4_1; -.
DR   InParanoid; P01733; -.
DR   OMA; WFSAEMT; -.
DR   PhylomeDB; P01733; -.
DR   PathwayCommons; P01733; -.
DR   Reactome; R-HSA-198933; Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
DR   Reactome; R-HSA-202424; Downstream TCR signaling.
DR   Reactome; R-HSA-202427; Phosphorylation of CD3 and TCR zeta chains.
DR   Reactome; R-HSA-202430; Translocation of ZAP-70 to Immunological synapse.
DR   Reactome; R-HSA-202433; Generation of second messenger molecules.
DR   Reactome; R-HSA-389948; PD-1 signaling.
DR   ChiTaRS; TRBV12-3; human.
DR   Pharos; P01733; Tdark.
DR   PRO; PR:P01733; -.
DR   Proteomes; UP000005640; Chromosome 7.
DR   RNAct; P01733; Protein.
DR   Bgee; ENSG00000274752; Expressed in lymph node and 82 other cell types or tissues.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0042101; C:T cell receptor complex; IEA:UniProtKB-KW.
DR   GO; GO:0042287; F:MHC protein binding; NAS:UniProtKB.
DR   GO; GO:0042605; F:peptide antigen binding; NAS:UniProtKB.
DR   GO; GO:0002250; P:adaptive immune response; IEA:UniProtKB-KW.
DR   GO; GO:0007166; P:cell surface receptor signaling pathway; IBA:GO_Central.
DR   GO; GO:0006955; P:immune response; NAS:UniProtKB.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR   InterPro; IPR007110; Ig-like_dom.
DR   InterPro; IPR036179; Ig-like_dom_sf.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR013106; Ig_V-set.
DR   PANTHER; PTHR23268:SF122; T CELL RECEPTOR BETA VARIABLE 12-3-RELATED; 1.
DR   PANTHER; PTHR23268; T-CELL RECEPTOR BETA CHAIN; 1.
DR   Pfam; PF07686; V-set; 1.
DR   SMART; SM00406; IGv; 1.
DR   SUPFAM; SSF48726; Immunoglobulin; 1.
DR   PROSITE; PS50835; IG_LIKE; 1.
PE   1: Evidence at protein level;
KW   Adaptive immunity; Cell membrane; Disulfide bond; Glycoprotein; Immunity;
KW   Immunoglobulin domain; Membrane; Receptor; Reference proteome; Signal;
KW   T cell receptor.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..115
FT                   /note="T cell receptor beta variable 12-3"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_5014017119"
FT   DOMAIN          22..>115
FT                   /note="Ig-like"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT   CARBOHYD        90
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        42..111
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT   CONFLICT        115
FT                   /note="L -> F (in Ref. 1)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        115
FT                   /note="L -> LA (in Ref. 3; AAC13336)"
FT                   /evidence="ECO:0000305"
FT   NON_TER         115
SQ   SEQUENCE   115 AA;  12997 MW;  D9BF12E944882F14 CRC64;
     MDSWTFCCVS LCILVAKHTD AGVIQSPRHE VTEMGQEVTL RCKPISGHNS LFWYRQTMMR
     GLELLIYFNN NVPIDDSGMP EDRFSAKMPN ASFSTLKIQP SEPRDSAVYF CASSL
//