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Protein

T-cell surface glycoprotein CD8 alpha chain

Gene

Cd8a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

GO - Molecular functioni

  • protein homodimerization activity Source: MGI
  • protein kinase binding Source: Ensembl

GO - Biological processi

  • cell surface receptor signaling pathway Source: MGI
  • cytotoxic T cell differentiation Source: MGI
  • defense response to virus Source: MGI
  • positive regulation of calcium-mediated signaling Source: MGI
  • T cell mediated immunity Source: MGI

Keywordsi

Biological processAdaptive immunity, Immunity

Names & Taxonomyi

Protein namesi
Recommended name:
T-cell surface glycoprotein CD8 alpha chain
Alternative name(s):
T-cell surface glycoprotein Lyt-2
CD_antigen: CD8a
Gene namesi
Name:Cd8a
Synonyms:Lyt-2, Lyt2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:88346. Cd8a.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini28 – 196ExtracellularSequence analysisAdd BLAST169
Transmembranei197 – 217HelicalSequence analysisAdd BLAST21
Topological domaini218 – 247CytoplasmicSequence analysisAdd BLAST30

GO - Cellular componenti

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Add BLAST27
ChainiPRO_000001463928 – 247T-cell surface glycoprotein CD8 alpha chainAdd BLAST220

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi53 ↔ 129PROSITE-ProRule annotation1 Publication
Glycosylationi69N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi97N-linked (GlcNAc...) asparagine1
Glycosylationi150N-linked (GlcNAc...) asparagine1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiP01731.
MaxQBiP01731.
PaxDbiP01731.
PRIDEiP01731.

PTM databases

PhosphoSitePlusiP01731.

Expressioni

Gene expression databases

BgeeiENSMUSG00000053977.
CleanExiMM_CD8A.
ExpressionAtlasiP01731. baseline and differential.
GenevisibleiP01731. MM.

Interactioni

Subunit structurei

In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
LckP062402EBI-1433,EBI-1401

GO - Molecular functioni

  • protein homodimerization activity Source: MGI
  • protein kinase binding Source: Ensembl

Protein-protein interaction databases

IntActiP01731. 4 interactors.
MINTiMINT-7895757.
STRINGi10090.ENSMUSP00000068123.

Structurei

Secondary structure

1247
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi33 – 39Combined sources7
Beta strandi41 – 43Combined sources3
Beta strandi49 – 57Combined sources9
Beta strandi63 – 71Combined sources9
Beta strandi78 – 83Combined sources6
Beta strandi85 – 87Combined sources3
Beta strandi90 – 92Combined sources3
Turni94 – 97Combined sources4
Helixi98 – 100Combined sources3
Beta strandi102 – 107Combined sources6
Turni108 – 110Combined sources3
Beta strandi111 – 118Combined sources8
Helixi121 – 123Combined sources3
Beta strandi125 – 133Combined sources9
Beta strandi136 – 139Combined sources4
Beta strandi143 – 147Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BQHX-ray2.80G/H/I/K28-156[»]
1NEZX-ray2.10G/H28-149[»]
2ARJX-ray2.88Q/R28-150[»]
2ATPX-ray2.40A/C28-149[»]
3B9KX-ray2.70A/E28-151[»]
3DMMX-ray2.60C23-188[»]
ProteinModelPortaliP01731.
SMRiP01731.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP01731.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 139Ig-like V-typeAdd BLAST112

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IWN3. Eukaryota.
ENOG410Z6EI. LUCA.
GeneTreeiENSGT00510000048935.
HOGENOMiHOG000004794.
HOVERGENiHBG008488.
InParanoidiP01731.
KOiK06458.
OMAiRVCKCPR.
OrthoDBiEOG091G0YIT.
PhylomeDBiP01731.
TreeFamiTF336070.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiView protein in InterPro
IPR015468. CD8_asu.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
PANTHERiPTHR10441. PTHR10441. 1 hit.
PfamiView protein in Pfam
PF07686. V-set. 1 hit.
SMARTiView protein in SMART
SM00409. IG. 1 hit.
SM00406. IGv. 1 hit.
SUPFAMiSSF48726. SSF48726. 1 hit.
PROSITEiView protein in PROSITE
PS50835. IG_LIKE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. Alternative splicing involves excision of the transmembrane or cytoplasmic domains.
Isoform 1 (identifier: P01731-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASPLTRFLS LNLLLLGESI ILGSGEAKPQ APELRIFPKK MDAELGQKVD
60 70 80 90 100
LVCEVLGSVS QGCSWLFQNS SSKLPQPTFV VYMASSHNKI TWDEKLNSSK
110 120 130 140 150
LFSAMRDTNN KYVLTLNKFS KENEGYYFCS VISNSVMYFS SVVPVLQKVN
160 170 180 190 200
STTTKPVLRT PSPVHPTGTS QPQRPEDCRP RGSVKGTGLD FACDIYIWAP
210 220 230 240
LAGICVALLL SLIITLICYH RSRKRVCKCP RPLVRQEGKP RPSEKIV
Length:247
Mass (Da):27,456
Last modified:July 21, 1986 - v1
Checksum:i22D0D78ECEC3EA04
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti81Missing in AAA39477 (PubMed:3495785).Curated1
Sequence conflicti81Missing in CAA68352 (PubMed:3495785).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti105M → V in strain: C.AKR. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12825 mRNA. Translation: AAA39476.1.
M16981 mRNA. Translation: AAA39477.1. Different termination.
M12052 mRNA. Translation: AAA39478.1.
Y00157 Genomic DNA. Translation: CAA68352.2.
M22064 Genomic DNA. Translation: AAA39665.1.
M12977
, M12819, M12975, M12976 Genomic DNA. Translation: AAA39475.1.
CCDSiCCDS39507.1. [P01731-1]
PIRiA01998. RWMST2.
A29523.
A34954.
RefSeqiNP_001074579.1. NM_001081110.2. [P01731-1]
UniGeneiMm.1858.

Genome annotation databases

EnsembliENSMUST00000066747; ENSMUSP00000068123; ENSMUSG00000053977. [P01731-1]
GeneIDi12525.
KEGGimmu:12525.
UCSCiuc009cgr.1. mouse. [P01731-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiCD8A_MOUSE
AccessioniPrimary (citable) accession number: P01731
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: June 7, 2017
This is version 168 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references