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P01731

- CD8A_MOUSE

UniProt

P01731 - CD8A_MOUSE

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Protein

T-cell surface glycoprotein CD8 alpha chain

Gene

Cd8a

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

GO - Molecular functioni

  1. protein homodimerization activity Source: MGI

GO - Biological processi

  1. cell surface receptor signaling pathway Source: MGI
  2. cytotoxic T cell differentiation Source: MGI
  3. defense response to virus Source: MGI
  4. positive regulation of calcium-mediated signaling Source: MGI
  5. T cell mediated immunity Source: MGI
Complete GO annotation...

Keywords - Biological processi

Adaptive immunity, Immunity

Enzyme and pathway databases

ReactomeiREACT_202937. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.

Names & Taxonomyi

Protein namesi
Recommended name:
T-cell surface glycoprotein CD8 alpha chain
Alternative name(s):
T-cell surface glycoprotein Lyt-2
CD_antigen: CD8a
Gene namesi
Name:Cd8a
Synonyms:Lyt-2, Lyt2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:88346. Cd8a.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini28 – 196169ExtracellularSequence AnalysisAdd
BLAST
Transmembranei197 – 21721HelicalSequence AnalysisAdd
BLAST
Topological domaini218 – 24730CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cell surface Source: MGI
  2. external side of plasma membrane Source: MGI
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Add
BLAST
Chaini28 – 247220T-cell surface glycoprotein CD8 alpha chainPRO_0000014639Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi53 ↔ 1291 PublicationPROSITE-ProRule annotation
Glycosylationi69 – 691N-linked (GlcNAc...)1 Publication
Glycosylationi97 – 971N-linked (GlcNAc...)
Glycosylationi150 – 1501N-linked (GlcNAc...)

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP01731.
PRIDEiP01731.

PTM databases

PhosphoSiteiP01731.

Expressioni

Gene expression databases

BgeeiP01731.
CleanExiMM_CD8A.
ExpressionAtlasiP01731. baseline and differential.
GenevestigatoriP01731.

Interactioni

Subunit structurei

In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
LckP062402EBI-1433,EBI-1401

Protein-protein interaction databases

IntActiP01731. 4 interactions.
MINTiMINT-7895757.

Structurei

Secondary structure

1
247
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi33 – 397Combined sources
Beta strandi41 – 433Combined sources
Beta strandi49 – 579Combined sources
Beta strandi63 – 719Combined sources
Beta strandi78 – 836Combined sources
Beta strandi85 – 873Combined sources
Beta strandi90 – 923Combined sources
Turni94 – 974Combined sources
Helixi98 – 1003Combined sources
Beta strandi102 – 1076Combined sources
Turni108 – 1103Combined sources
Beta strandi111 – 1188Combined sources
Helixi121 – 1233Combined sources
Beta strandi125 – 1339Combined sources
Beta strandi136 – 1394Combined sources
Beta strandi143 – 1475Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BQHX-ray2.80G/H/I/K28-156[»]
1NEZX-ray2.10G/H28-149[»]
2ARJX-ray2.88Q/R28-150[»]
2ATPX-ray2.40A/C28-149[»]
3B9KX-ray2.70A/E28-151[»]
3DMMX-ray2.60C23-188[»]
ProteinModelPortaliP01731.
SMRiP01731. Positions 28-147.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP01731.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 139112Ig-like V-typeAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG46810.
GeneTreeiENSGT00510000048935.
HOGENOMiHOG000004794.
HOVERGENiHBG008488.
InParanoidiP01731.
KOiK06458.
OMAiRVCKCPR.
OrthoDBiEOG7N37F3.
PhylomeDBiP01731.
TreeFamiTF336070.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR015468. CD8_asu.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013151. Immunoglobulin.
[Graphical view]
PANTHERiPTHR10441. PTHR10441. 1 hit.
PfamiPF00047. ig. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. Alternative splicing involves excision of the transmembrane or cytoplasmic domains.

Isoform 1 (identifier: P01731-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASPLTRFLS LNLLLLGESI ILGSGEAKPQ APELRIFPKK MDAELGQKVD
60 70 80 90 100
LVCEVLGSVS QGCSWLFQNS SSKLPQPTFV VYMASSHNKI TWDEKLNSSK
110 120 130 140 150
LFSAMRDTNN KYVLTLNKFS KENEGYYFCS VISNSVMYFS SVVPVLQKVN
160 170 180 190 200
STTTKPVLRT PSPVHPTGTS QPQRPEDCRP RGSVKGTGLD FACDIYIWAP
210 220 230 240
LAGICVALLL SLIITLICYH RSRKRVCKCP RPLVRQEGKP RPSEKIV
Length:247
Mass (Da):27,456
Last modified:July 21, 1986 - v1
Checksum:i22D0D78ECEC3EA04
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti81 – 811Missing in AAA39477. (PubMed:3495785)Curated
Sequence conflicti81 – 811Missing in CAA68352. (PubMed:3495785)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti105 – 1051M → V in strain: C.AKR.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12825 mRNA. Translation: AAA39476.1.
M16981 mRNA. Translation: AAA39477.1. Different termination.
M12052 mRNA. Translation: AAA39478.1.
Y00157 Genomic DNA. Translation: CAA68352.2.
M22064 Genomic DNA. Translation: AAA39665.1.
M12977
, M12819, M12975, M12976 Genomic DNA. Translation: AAA39475.1.
CCDSiCCDS39507.1. [P01731-1]
PIRiA01998. RWMST2.
A29523.
A34954.
RefSeqiNP_001074579.1. NM_001081110.2. [P01731-1]
XP_006505527.1. XM_006505464.1. [P01731-1]
UniGeneiMm.1858.

Genome annotation databases

EnsembliENSMUST00000066747; ENSMUSP00000068123; ENSMUSG00000053977. [P01731-1]
GeneIDi12525.
KEGGimmu:12525.
UCSCiuc009cgr.1. mouse. [P01731-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12825 mRNA. Translation: AAA39476.1 .
M16981 mRNA. Translation: AAA39477.1 . Different termination.
M12052 mRNA. Translation: AAA39478.1 .
Y00157 Genomic DNA. Translation: CAA68352.2 .
M22064 Genomic DNA. Translation: AAA39665.1 .
M12977
, M12819 , M12975 , M12976 Genomic DNA. Translation: AAA39475.1 .
CCDSi CCDS39507.1. [P01731-1 ]
PIRi A01998. RWMST2.
A29523.
A34954.
RefSeqi NP_001074579.1. NM_001081110.2. [P01731-1 ]
XP_006505527.1. XM_006505464.1. [P01731-1 ]
UniGenei Mm.1858.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1BQH X-ray 2.80 G/H/I/K 28-156 [» ]
1NEZ X-ray 2.10 G/H 28-149 [» ]
2ARJ X-ray 2.88 Q/R 28-150 [» ]
2ATP X-ray 2.40 A/C 28-149 [» ]
3B9K X-ray 2.70 A/E 28-151 [» ]
3DMM X-ray 2.60 C 23-188 [» ]
ProteinModelPortali P01731.
SMRi P01731. Positions 28-147.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P01731. 4 interactions.
MINTi MINT-7895757.

PTM databases

PhosphoSitei P01731.

Proteomic databases

MaxQBi P01731.
PRIDEi P01731.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000066747 ; ENSMUSP00000068123 ; ENSMUSG00000053977 . [P01731-1 ]
GeneIDi 12525.
KEGGi mmu:12525.
UCSCi uc009cgr.1. mouse. [P01731-1 ]

Organism-specific databases

CTDi 925.
MGIi MGI:88346. Cd8a.

Phylogenomic databases

eggNOGi NOG46810.
GeneTreei ENSGT00510000048935.
HOGENOMi HOG000004794.
HOVERGENi HBG008488.
InParanoidi P01731.
KOi K06458.
OMAi RVCKCPR.
OrthoDBi EOG7N37F3.
PhylomeDBi P01731.
TreeFami TF336070.

Enzyme and pathway databases

Reactomei REACT_202937. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.

Miscellaneous databases

EvolutionaryTracei P01731.
NextBioi 281546.
PROi P01731.
SOURCEi Search...

Gene expression databases

Bgeei P01731.
CleanExi MM_CD8A.
ExpressionAtlasi P01731. baseline and differential.
Genevestigatori P01731.

Family and domain databases

Gene3Di 2.60.40.10. 1 hit.
InterProi IPR015468. CD8_asu.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013151. Immunoglobulin.
[Graphical view ]
PANTHERi PTHR10441. PTHR10441. 1 hit.
Pfami PF00047. ig. 1 hit.
[Graphical view ]
SMARTi SM00409. IG. 1 hit.
[Graphical view ]
PROSITEi PS50835. IG_LIKE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of Lyt-2, a membrane glycoprotein marking a subset of mouse T lymphocytes: molecular homology to its human counterpart, Leu-2/T8, and to immunoglobulin variable regions."
    Nakauchi H., Nolan G.P., Hsu C., Huang H.S., Kavathas P., Herzenberg L.A.
    Proc. Natl. Acad. Sci. U.S.A. 82:5126-5130(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  2. "Two Lyt-2 polypeptides arise from a single gene by alternative splicing patterns of mRNA."
    Zamoyska R., Vollmer A.C., Sizer K.C., Liaw C.W., Parnes J.R.
    Cell 43:153-163(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Isolation and characterization of the gene for the murine T cell differentiation antigen and immunoglobulin-related molecule, Lyt-2."
    Nakauchi H., Tagawa M., Nolan G.P., Herzenberg L.A.
    Nucleic Acids Res. 15:4337-4347(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Nucleotide sequence analysis of the C.AKR Lyt-2a gene: structural polymorphism in alleles encoding the Lyt-2.1 T-cell surface alloantigen."
    Youn H.J., Harriss J.V., Gottlieb P.D.
    Immunogenetics 28:345-352(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: C.AKR.
  5. "Structure, sequence, and polymorphism of the Lyt-2 T cell differentiation antigen gene."
    Liaw C.W., Zamoyska R., Parnes J.R.
    J. Immunol. 137:1037-1043(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  6. "Structural basis of CD8 coreceptor function revealed by crystallographic analysis of a murine CD8alphaalpha ectodomain fragment in complex with H-2Kb."
    Kern P.S., Teng M.K., Smolyar A., Liu J.H., Liu J., Hussey R.E., Spoerl R., Chang H.-C., Reinherz E.L., Wang J.-H.
    Immunity 9:519-530(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 28-152 IN COMPLEX WITH H-2KB.
  7. "The crystal structure of CD8 in complex with YTS156.7.7 Fab and interaction with other CD8 antibodies define the binding mode of CD8 alphabeta to MHC class I."
    Shore D.A., Issafras H., Landais E., Teyton L., Wilson I.A.
    J. Mol. Biol. 384:1190-1202(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 28-151 IN COMPLEX WITH CD8B AND ANTIBODY, SUBUNIT, DISULFIDE BOND, GLYCOSYLATION AT ASN-69.

Entry informationi

Entry nameiCD8A_MOUSE
AccessioniPrimary (citable) accession number: P01731
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 26, 2014
This is version 152 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3