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P01731

- CD8A_MOUSE

UniProt

P01731 - CD8A_MOUSE

Protein

T-cell surface glycoprotein CD8 alpha chain

Gene

Cd8a

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 150 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. protein homodimerization activity Source: MGI

    GO - Biological processi

    1. cell surface receptor signaling pathway Source: MGI
    2. cytotoxic T cell differentiation Source: MGI
    3. defense response to virus Source: MGI
    4. positive regulation of calcium-mediated signaling Source: MGI
    5. T cell mediated immunity Source: MGI

    Keywords - Biological processi

    Adaptive immunity, Immunity

    Enzyme and pathway databases

    ReactomeiREACT_202937. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    T-cell surface glycoprotein CD8 alpha chain
    Alternative name(s):
    T-cell surface glycoprotein Lyt-2
    CD_antigen: CD8a
    Gene namesi
    Name:Cd8a
    Synonyms:Lyt-2, Lyt2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:88346. Cd8a.

    Subcellular locationi

    GO - Cellular componenti

    1. cell surface Source: MGI
    2. external side of plasma membrane Source: MGI
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727Add
    BLAST
    Chaini28 – 247220T-cell surface glycoprotein CD8 alpha chainPRO_0000014639Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi53 ↔ 1291 PublicationPROSITE-ProRule annotation
    Glycosylationi69 – 691N-linked (GlcNAc...)1 Publication
    Glycosylationi97 – 971N-linked (GlcNAc...)
    Glycosylationi150 – 1501N-linked (GlcNAc...)

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiP01731.
    PRIDEiP01731.

    PTM databases

    PhosphoSiteiP01731.

    Expressioni

    Gene expression databases

    ArrayExpressiP01731.
    BgeeiP01731.
    CleanExiMM_CD8A.
    GenevestigatoriP01731.

    Interactioni

    Subunit structurei

    In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    LckP062402EBI-1433,EBI-1401

    Protein-protein interaction databases

    IntActiP01731. 4 interactions.
    MINTiMINT-7895757.

    Structurei

    Secondary structure

    1
    247
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi33 – 397
    Beta strandi41 – 433
    Beta strandi49 – 579
    Beta strandi63 – 719
    Beta strandi78 – 836
    Beta strandi85 – 873
    Beta strandi90 – 923
    Turni94 – 974
    Helixi98 – 1003
    Beta strandi102 – 1076
    Turni108 – 1103
    Beta strandi111 – 1188
    Helixi121 – 1233
    Beta strandi125 – 1339
    Beta strandi136 – 1394
    Beta strandi143 – 1475

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1BQHX-ray2.80G/H/I/K28-156[»]
    1NEZX-ray2.10G/H28-149[»]
    2ARJX-ray2.88Q/R28-150[»]
    2ATPX-ray2.40A/C28-149[»]
    3B9KX-ray2.70A/E28-151[»]
    3DMMX-ray2.60C23-188[»]
    ProteinModelPortaliP01731.
    SMRiP01731. Positions 28-147.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP01731.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini28 – 196169ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini218 – 24730CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei197 – 21721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini28 – 139112Ig-like V-typeAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG46810.
    GeneTreeiENSGT00510000048935.
    HOGENOMiHOG000004794.
    HOVERGENiHBG008488.
    InParanoidiP01731.
    KOiK06458.
    OMAiRVCKCPR.
    OrthoDBiEOG7N37F3.
    PhylomeDBiP01731.
    TreeFamiTF336070.

    Family and domain databases

    Gene3Di2.60.40.10. 1 hit.
    InterProiIPR015468. CD8_asu.
    IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR003599. Ig_sub.
    IPR013151. Immunoglobulin.
    [Graphical view]
    PANTHERiPTHR10441. PTHR10441. 1 hit.
    PfamiPF00047. ig. 1 hit.
    [Graphical view]
    SMARTiSM00409. IG. 1 hit.
    [Graphical view]
    PROSITEiPS50835. IG_LIKE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. Alternative splicing involves excision of the transmembrane or cytoplasmic domains.

    Isoform 1 (identifier: P01731-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MASPLTRFLS LNLLLLGESI ILGSGEAKPQ APELRIFPKK MDAELGQKVD    50
    LVCEVLGSVS QGCSWLFQNS SSKLPQPTFV VYMASSHNKI TWDEKLNSSK 100
    LFSAMRDTNN KYVLTLNKFS KENEGYYFCS VISNSVMYFS SVVPVLQKVN 150
    STTTKPVLRT PSPVHPTGTS QPQRPEDCRP RGSVKGTGLD FACDIYIWAP 200
    LAGICVALLL SLIITLICYH RSRKRVCKCP RPLVRQEGKP RPSEKIV 247
    Length:247
    Mass (Da):27,456
    Last modified:July 21, 1986 - v1
    Checksum:i22D0D78ECEC3EA04
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti81 – 811Missing in AAA39477. (PubMed:3495785)Curated
    Sequence conflicti81 – 811Missing in CAA68352. (PubMed:3495785)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti105 – 1051M → V in strain: C.AKR.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M12825 mRNA. Translation: AAA39476.1.
    M16981 mRNA. Translation: AAA39477.1. Different termination.
    M12052 mRNA. Translation: AAA39478.1.
    Y00157 Genomic DNA. Translation: CAA68352.2.
    M22064 Genomic DNA. Translation: AAA39665.1.
    M12977
    , M12819, M12975, M12976 Genomic DNA. Translation: AAA39475.1.
    CCDSiCCDS39507.1. [P01731-1]
    PIRiA01998. RWMST2.
    A29523.
    A34954.
    RefSeqiNP_001074579.1. NM_001081110.2. [P01731-1]
    XP_006505527.1. XM_006505464.1. [P01731-1]
    UniGeneiMm.1858.

    Genome annotation databases

    EnsembliENSMUST00000066747; ENSMUSP00000068123; ENSMUSG00000053977. [P01731-1]
    GeneIDi12525.
    KEGGimmu:12525.
    UCSCiuc009cgr.1. mouse. [P01731-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M12825 mRNA. Translation: AAA39476.1 .
    M16981 mRNA. Translation: AAA39477.1 . Different termination.
    M12052 mRNA. Translation: AAA39478.1 .
    Y00157 Genomic DNA. Translation: CAA68352.2 .
    M22064 Genomic DNA. Translation: AAA39665.1 .
    M12977
    , M12819 , M12975 , M12976 Genomic DNA. Translation: AAA39475.1 .
    CCDSi CCDS39507.1. [P01731-1 ]
    PIRi A01998. RWMST2.
    A29523.
    A34954.
    RefSeqi NP_001074579.1. NM_001081110.2. [P01731-1 ]
    XP_006505527.1. XM_006505464.1. [P01731-1 ]
    UniGenei Mm.1858.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1BQH X-ray 2.80 G/H/I/K 28-156 [» ]
    1NEZ X-ray 2.10 G/H 28-149 [» ]
    2ARJ X-ray 2.88 Q/R 28-150 [» ]
    2ATP X-ray 2.40 A/C 28-149 [» ]
    3B9K X-ray 2.70 A/E 28-151 [» ]
    3DMM X-ray 2.60 C 23-188 [» ]
    ProteinModelPortali P01731.
    SMRi P01731. Positions 28-147.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P01731. 4 interactions.
    MINTi MINT-7895757.

    PTM databases

    PhosphoSitei P01731.

    Proteomic databases

    MaxQBi P01731.
    PRIDEi P01731.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000066747 ; ENSMUSP00000068123 ; ENSMUSG00000053977 . [P01731-1 ]
    GeneIDi 12525.
    KEGGi mmu:12525.
    UCSCi uc009cgr.1. mouse. [P01731-1 ]

    Organism-specific databases

    CTDi 925.
    MGIi MGI:88346. Cd8a.

    Phylogenomic databases

    eggNOGi NOG46810.
    GeneTreei ENSGT00510000048935.
    HOGENOMi HOG000004794.
    HOVERGENi HBG008488.
    InParanoidi P01731.
    KOi K06458.
    OMAi RVCKCPR.
    OrthoDBi EOG7N37F3.
    PhylomeDBi P01731.
    TreeFami TF336070.

    Enzyme and pathway databases

    Reactomei REACT_202937. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.

    Miscellaneous databases

    EvolutionaryTracei P01731.
    NextBioi 281546.
    PROi P01731.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P01731.
    Bgeei P01731.
    CleanExi MM_CD8A.
    Genevestigatori P01731.

    Family and domain databases

    Gene3Di 2.60.40.10. 1 hit.
    InterProi IPR015468. CD8_asu.
    IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR003599. Ig_sub.
    IPR013151. Immunoglobulin.
    [Graphical view ]
    PANTHERi PTHR10441. PTHR10441. 1 hit.
    Pfami PF00047. ig. 1 hit.
    [Graphical view ]
    SMARTi SM00409. IG. 1 hit.
    [Graphical view ]
    PROSITEi PS50835. IG_LIKE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of Lyt-2, a membrane glycoprotein marking a subset of mouse T lymphocytes: molecular homology to its human counterpart, Leu-2/T8, and to immunoglobulin variable regions."
      Nakauchi H., Nolan G.P., Hsu C., Huang H.S., Kavathas P., Herzenberg L.A.
      Proc. Natl. Acad. Sci. U.S.A. 82:5126-5130(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
    2. "Two Lyt-2 polypeptides arise from a single gene by alternative splicing patterns of mRNA."
      Zamoyska R., Vollmer A.C., Sizer K.C., Liaw C.W., Parnes J.R.
      Cell 43:153-163(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Isolation and characterization of the gene for the murine T cell differentiation antigen and immunoglobulin-related molecule, Lyt-2."
      Nakauchi H., Tagawa M., Nolan G.P., Herzenberg L.A.
      Nucleic Acids Res. 15:4337-4347(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "Nucleotide sequence analysis of the C.AKR Lyt-2a gene: structural polymorphism in alleles encoding the Lyt-2.1 T-cell surface alloantigen."
      Youn H.J., Harriss J.V., Gottlieb P.D.
      Immunogenetics 28:345-352(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: C.AKR.
    5. "Structure, sequence, and polymorphism of the Lyt-2 T cell differentiation antigen gene."
      Liaw C.W., Zamoyska R., Parnes J.R.
      J. Immunol. 137:1037-1043(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    6. "Structural basis of CD8 coreceptor function revealed by crystallographic analysis of a murine CD8alphaalpha ectodomain fragment in complex with H-2Kb."
      Kern P.S., Teng M.K., Smolyar A., Liu J.H., Liu J., Hussey R.E., Spoerl R., Chang H.-C., Reinherz E.L., Wang J.-H.
      Immunity 9:519-530(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 28-152 IN COMPLEX WITH H-2KB.
    7. "The crystal structure of CD8 in complex with YTS156.7.7 Fab and interaction with other CD8 antibodies define the binding mode of CD8 alphabeta to MHC class I."
      Shore D.A., Issafras H., Landais E., Teyton L., Wilson I.A.
      J. Mol. Biol. 384:1190-1202(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 28-151 IN COMPLEX WITH CD8B AND ANTIBODY, SUBUNIT, DISULFIDE BOND, GLYCOSYLATION AT ASN-69.

    Entry informationi

    Entry nameiCD8A_MOUSE
    AccessioniPrimary (citable) accession number: P01731
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 150 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3