T-cell surface glycoprotein CD8 alpha chain precursor

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P01731 (CD8A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 137. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
T-cell surface glycoprotein CD8 alpha chain

Alternative name(s):
T-cell surface glycoprotein Lyt-2
CD_antigen=CD8a

Gene names

Name:	Cd8a
Synonyms:	Lyt-2, Lyt2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	247 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Subunit structure	In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Ref.7
Subcellular location	Membrane; Single-pass type I membrane protein.
Sequence similarities	Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Ontologies

Keywords
Biological process	Adaptive immunity Immunity
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Immunoglobulin domain Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	T cell mediated immunity Inferred from mutant phenotype PubMed 1673361. Source: MGI cell surface receptor signaling pathway Inferred from direct assay PubMed 9208839. Source: MGI cytotoxic T cell differentiation Inferred from mutant phenotype PubMed 1673361. Source: MGI defense response to virus Inferred from direct assay PubMed 17277142. Source: MGI positive regulation of calcium-mediated signaling Inferred from direct assay PubMed 9208839. Source: MGI
Cellular_component	external side of plasma membrane Inferred from direct assay PubMed 10072077 PubMed 10648399 PubMed 10704459 PubMed 10943842 PubMed 14609575 PubMed 15240714 PubMed 15294943 PubMed 15322158 PubMed 15728238 PubMed 15795238 PubMed 16287714 PubMed 16455951 PubMed 1673361 PubMed 16973387 PubMed 17082577 PubMed 17277142 PubMed 17357106 PubMed 18776904 PubMed 18836449 PubMed 18984740 PubMed 19349303 PubMed 19723499 PubMed 19838199 PubMed 19934022 PubMed 20709950 PubMed 21460847 PubMed 21508411 PubMed 22072979 PubMed 6610701 PubMed 7589135 PubMed 8688082 PubMed 8755570 PubMed 8788039 PubMed 9208839. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	protein homodimerization activity Inferred from direct assay PubMed 15749886. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select] Note: A number of isoforms are produced. Alternative splicing involves excision of the transmembrane or cytoplasmic domains.

Isoform 1 (identifier: P01731-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 27

Chain

28 – 247

220

T-cell surface glycoprotein CD8 alpha chain

PRO_0000014639

Regions

Topological domain

28 – 196

169

Extracellular Potential

Transmembrane

197 – 217

Helical; Potential

Topological domain

218 – 247

Cytoplasmic Potential

Domain

28 – 139

112

Ig-like V-type

Amino acid modifications

Glycosylation

N-linked (GlcNAc...) Ref.7

Glycosylation

N-linked (GlcNAc...)

Glycosylation

150

N-linked (GlcNAc...)

Disulfide bond

53 ↔ 129

Ref.7

Natural variations

Natural variant

105

M → V in strain: C.AKR.

Experimental info

Sequence conflict

Missing in AAA39477. Ref.3

Sequence conflict

Missing in CAA68352. Ref.3

Secondary structure

247

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Isoform 1 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 22D0D78ECEC3EA04
Show »

FASTA

247

27,456

        10         20         30         40         50         60 
MASPLTRFLS LNLLLLGESI ILGSGEAKPQ APELRIFPKK MDAELGQKVD LVCEVLGSVS 

        70         80         90        100        110        120 
QGCSWLFQNS SSKLPQPTFV VYMASSHNKI TWDEKLNSSK LFSAMRDTNN KYVLTLNKFS 

       130        140        150        160        170        180 
KENEGYYFCS VISNSVMYFS SVVPVLQKVN STTTKPVLRT PSPVHPTGTS QPQRPEDCRP 

       190        200        210        220        230        240 
RGSVKGTGLD FACDIYIWAP LAGICVALLL SLIITLICYH RSRKRVCKCP RPLVRQEGKP 


RPSEKIV

« Hide

References

[1]	"Molecular cloning of Lyt-2, a membrane glycoprotein marking a subset of mouse T lymphocytes: molecular homology to its human counterpart, Leu-2/T8, and to immunoglobulin variable regions." Nakauchi H., Nolan G.P., Hsu C., Huang H.S., Kavathas P., Herzenberg L.A. Proc. Natl. Acad. Sci. U.S.A. 82:5126-5130(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c.
[2]	"Two Lyt-2 polypeptides arise from a single gene by alternative splicing patterns of mRNA." Zamoyska R., Vollmer A.C., Sizer K.C., Liaw C.W., Parnes J.R. Cell 43:153-163(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Isolation and characterization of the gene for the murine T cell differentiation antigen and immunoglobulin-related molecule, Lyt-2." Nakauchi H., Tagawa M., Nolan G.P., Herzenberg L.A. Nucleic Acids Res. 15:4337-4347(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Nucleotide sequence analysis of the C.AKR Lyt-2a gene: structural polymorphism in alleles encoding the Lyt-2.1 T-cell surface alloantigen." Youn H.J., Harriss J.V., Gottlieb P.D. Immunogenetics 28:345-352(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C.AKR.
[5]	"Structure, sequence, and polymorphism of the Lyt-2 T cell differentiation antigen gene." Liaw C.W., Zamoyska R., Parnes J.R. J. Immunol. 137:1037-1043(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]	"Structural basis of CD8 coreceptor function revealed by crystallographic analysis of a murine CD8alphaalpha ectodomain fragment in complex with H-2Kb." Kern P.S., Teng M.K., Smolyar A., Liu J.H., Liu J., Hussey R.E., Spoerl R., Chang H.-C., Reinherz E.L., Wang J.-H. Immunity 9:519-530(1998) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 28-152 IN COMPLEX WITH H-2KB.
[7]	"The crystal structure of CD8 in complex with YTS156.7.7 Fab and interaction with other CD8 antibodies define the binding mode of CD8 alphabeta to MHC class I." Shore D.A., Issafras H., Landais E., Teyton L., Wilson I.A. J. Mol. Biol. 384:1190-1202(2008) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 28-151 IN COMPLEX WITH CD8B AND ANTIBODY, SUBUNIT, DISULFIDE BOND, GLYCOSYLATION AT ASN-69.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M12825 mRNA. Translation: AAA39476.1.
M16981 mRNA. Translation: AAA39477.1. Different termination.
M12052 mRNA. Translation: AAA39478.1.
Y00157 Genomic DNA. Translation: CAA68352.2.
M22064 Genomic DNA. Translation: AAA39665.1.
M12977

M12976 Genomic DNA. Translation: AAA39475.1.

IPI

IPI00108594.

PIR

RWMST2. A01998.
A29523.
A34954.

RefSeq

NP_001074579.1. NM_001081110.2.

UniGene

Mm.1858.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BQH	X-ray	2.80	G/H/I/K	28-149	[»]
1NEZ	X-ray	2.10	G/H	28-149	[»]
2ARJ	X-ray	2.88	Q/R	28-150	[»]
2ATP	X-ray	2.40	A/C	28-149	[»]
3B9K	X-ray	2.70	A/E	28-151	[»]
3DMM	X-ray	2.60	C	23-188	[»]

ProteinModelPortal

P01731.

SMR

P01731. Positions 28-147.

ModBase

Search...

Protein-protein interaction databases

IntAct

P01731. 2 interactions.

PTM databases

PhosphoSite

P01731.

Proteomic databases

PRIDE

P01731.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000066747; ENSMUSP00000068123; ENSMUSG00000053977.

GeneID

12525.

KEGG

mmu:12525.

UCSC

uc009cgr.1. mouse.

Organism-specific databases

CTD

925.

MGI

MGI:88346. Cd8a.

Phylogenomic databases

eggNOG

NOG46810.

GeneTree

ENSGT00510000048935.

HOGENOM

HOG000004794.

HOVERGEN

HBG008488.

InParanoid

P01731.

K06458.

OMA

RVCKCPR.

OrthoDB

EOG4JDH84.

Gene expression databases

ArrayExpress

P01731.

Bgee

P01731.

CleanEx

MM_CD8A.

Genevestigator

P01731.

GermOnline

ENSMUSG00000053977. Mus musculus.

Family and domain databases

Gene3D

2.60.40.10. 1 hit.

InterPro

IPR015468. CD8_asu.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013151. Immunoglobulin.
[Graphical view]

PANTHER

PTHR10441. PTHR10441. 1 hit.

Pfam

PF00047. ig. 1 hit.
[Graphical view]

SMART

SM00409. IG. 1 hit.
[Graphical view]

PROSITE

PS50835. IG_LIKE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P01731.

NextBio

281546.

SOURCE

Search...

Entry information

Entry name

CD8A_MOUSE

Accession

Primary (citable) accession number: P01731

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	April 3, 2013
This is version 137 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents