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P01731 (CD8A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 148. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
T-cell surface glycoprotein CD8 alpha chain
Alternative name(s):
T-cell surface glycoprotein Lyt-2
CD_antigen=CD8a
Gene names
Name:Cd8a
Synonyms:Lyt-2, Lyt2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length247 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Subunit structure

In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Ref.7

Subcellular location

Membrane; Single-pass type I membrane protein.

Sequence similarities

Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Ontologies

Keywords
   Biological processAdaptive immunity
Immunity
   Cellular componentMembrane
   Coding sequence diversityAlternative splicing
   DomainImmunoglobulin domain
Signal
Transmembrane
Transmembrane helix
   PTMDisulfide bond
Glycoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processT cell mediated immunity

Inferred from mutant phenotype PubMed 1673361. Source: MGI

cell surface receptor signaling pathway

Inferred from direct assay PubMed 9208839. Source: MGI

cytotoxic T cell differentiation

Inferred from mutant phenotype PubMed 1673361. Source: MGI

defense response to virus

Inferred from direct assay PubMed 17277142. Source: MGI

positive regulation of calcium-mediated signaling

Inferred from direct assay PubMed 9208839. Source: MGI

   Cellular_componentcell surface

Inferred from direct assay PubMed 14568922. Source: MGI

external side of plasma membrane

Inferred from direct assay PubMed 10072077PubMed 10648399PubMed 10704459PubMed 10943842PubMed 14609575PubMed 15240714PubMed 15294943PubMed 15322158PubMed 15728238PubMed 15795238PubMed 16287714PubMed 16455951PubMed 1673361PubMed 16973387PubMed 17082577PubMed 17277142PubMed 17357106PubMed 18776904PubMed 18836449PubMed 18984740PubMed 19349303PubMed 19723499PubMed 19838199PubMed 19934022PubMed 20709950PubMed 21460847PubMed 21508411PubMed 22072979PubMed 6610701PubMed 7589135PubMed 8688082PubMed 8755570PubMed 8788039PubMed 9208839. Source: MGI

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionprotein binding

Inferred from physical interaction PubMed 24034254PubMed 7664803. Source: IntAct

protein homodimerization activity

Inferred from direct assay PubMed 15749886. Source: MGI

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

LckP062402EBI-1433,EBI-1401

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. Alternative splicing involves excision of the transmembrane or cytoplasmic domains.
Isoform 1 (identifier: P01731-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727
Chain28 – 247220T-cell surface glycoprotein CD8 alpha chain
PRO_0000014639

Regions

Topological domain28 – 196169Extracellular Potential
Transmembrane197 – 21721Helical; Potential
Topological domain218 – 24730Cytoplasmic Potential
Domain28 – 139112Ig-like V-type

Amino acid modifications

Glycosylation691N-linked (GlcNAc...) Ref.7
Glycosylation971N-linked (GlcNAc...)
Glycosylation1501N-linked (GlcNAc...)
Disulfide bond53 ↔ 129 Ref.7

Natural variations

Natural variant1051M → V in strain: C.AKR.

Experimental info

Sequence conflict811Missing in AAA39477. Ref.3
Sequence conflict811Missing in CAA68352. Ref.3

Secondary structure

.............................. 247
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 22D0D78ECEC3EA04

FASTA24727,456
        10         20         30         40         50         60 
MASPLTRFLS LNLLLLGESI ILGSGEAKPQ APELRIFPKK MDAELGQKVD LVCEVLGSVS 

        70         80         90        100        110        120 
QGCSWLFQNS SSKLPQPTFV VYMASSHNKI TWDEKLNSSK LFSAMRDTNN KYVLTLNKFS 

       130        140        150        160        170        180 
KENEGYYFCS VISNSVMYFS SVVPVLQKVN STTTKPVLRT PSPVHPTGTS QPQRPEDCRP 

       190        200        210        220        230        240 
RGSVKGTGLD FACDIYIWAP LAGICVALLL SLIITLICYH RSRKRVCKCP RPLVRQEGKP 


RPSEKIV 

« Hide

References

[1]"Molecular cloning of Lyt-2, a membrane glycoprotein marking a subset of mouse T lymphocytes: molecular homology to its human counterpart, Leu-2/T8, and to immunoglobulin variable regions."
Nakauchi H., Nolan G.P., Hsu C., Huang H.S., Kavathas P., Herzenberg L.A.
Proc. Natl. Acad. Sci. U.S.A. 82:5126-5130(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
[2]"Two Lyt-2 polypeptides arise from a single gene by alternative splicing patterns of mRNA."
Zamoyska R., Vollmer A.C., Sizer K.C., Liaw C.W., Parnes J.R.
Cell 43:153-163(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Isolation and characterization of the gene for the murine T cell differentiation antigen and immunoglobulin-related molecule, Lyt-2."
Nakauchi H., Tagawa M., Nolan G.P., Herzenberg L.A.
Nucleic Acids Res. 15:4337-4347(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Nucleotide sequence analysis of the C.AKR Lyt-2a gene: structural polymorphism in alleles encoding the Lyt-2.1 T-cell surface alloantigen."
Youn H.J., Harriss J.V., Gottlieb P.D.
Immunogenetics 28:345-352(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: C.AKR.
[5]"Structure, sequence, and polymorphism of the Lyt-2 T cell differentiation antigen gene."
Liaw C.W., Zamoyska R., Parnes J.R.
J. Immunol. 137:1037-1043(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]"Structural basis of CD8 coreceptor function revealed by crystallographic analysis of a murine CD8alphaalpha ectodomain fragment in complex with H-2Kb."
Kern P.S., Teng M.K., Smolyar A., Liu J.H., Liu J., Hussey R.E., Spoerl R., Chang H.-C., Reinherz E.L., Wang J.-H.
Immunity 9:519-530(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 28-152 IN COMPLEX WITH H-2KB.
[7]"The crystal structure of CD8 in complex with YTS156.7.7 Fab and interaction with other CD8 antibodies define the binding mode of CD8 alphabeta to MHC class I."
Shore D.A., Issafras H., Landais E., Teyton L., Wilson I.A.
J. Mol. Biol. 384:1190-1202(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 28-151 IN COMPLEX WITH CD8B AND ANTIBODY, SUBUNIT, DISULFIDE BOND, GLYCOSYLATION AT ASN-69.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M12825 mRNA. Translation: AAA39476.1.
M16981 mRNA. Translation: AAA39477.1. Different termination.
M12052 mRNA. Translation: AAA39478.1.
Y00157 Genomic DNA. Translation: CAA68352.2.
M22064 Genomic DNA. Translation: AAA39665.1.
M12977 expand/collapse EMBL AC list , M12819, M12975, M12976 Genomic DNA. Translation: AAA39475.1.
CCDSCCDS39507.1. [P01731-1]
PIRRWMST2. A01998.
A29523.
A34954.
RefSeqNP_001074579.1. NM_001081110.2. [P01731-1]
XP_006505527.1. XM_006505464.1. [P01731-1]
UniGeneMm.1858.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1BQHX-ray2.80G/H/I/K28-156[»]
1NEZX-ray2.10G/H28-149[»]
2ARJX-ray2.88Q/R28-150[»]
2ATPX-ray2.40A/C28-149[»]
3B9KX-ray2.70A/E28-151[»]
3DMMX-ray2.60C23-188[»]
ProteinModelPortalP01731.
SMRP01731. Positions 28-147.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP01731. 4 interactions.
MINTMINT-7895757.

PTM databases

PhosphoSiteP01731.

Proteomic databases

MaxQBP01731.
PRIDEP01731.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000066747; ENSMUSP00000068123; ENSMUSG00000053977. [P01731-1]
GeneID12525.
KEGGmmu:12525.
UCSCuc009cgr.1. mouse. [P01731-1]

Organism-specific databases

CTD925.
MGIMGI:88346. Cd8a.

Phylogenomic databases

eggNOGNOG46810.
GeneTreeENSGT00510000048935.
HOGENOMHOG000004794.
HOVERGENHBG008488.
InParanoidP01731.
KOK06458.
OMARVCKCPR.
OrthoDBEOG7N37F3.
PhylomeDBP01731.
TreeFamTF336070.

Gene expression databases

ArrayExpressP01731.
BgeeP01731.
CleanExMM_CD8A.
GenevestigatorP01731.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
InterProIPR015468. CD8_asu.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013151. Immunoglobulin.
[Graphical view]
PANTHERPTHR10441. PTHR10441. 1 hit.
PfamPF00047. ig. 1 hit.
[Graphical view]
SMARTSM00409. IG. 1 hit.
[Graphical view]
PROSITEPS50835. IG_LIKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP01731.
NextBio281546.
PROP01731.
SOURCESearch...

Entry information

Entry nameCD8A_MOUSE
AccessionPrimary (citable) accession number: P01731
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: July 9, 2014
This is version 148 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot