P01631 (KV2A7_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Ig kappa chain V-II region 26-10
Organism	Mus musculus (Mouse)
Taxonomic identifier	10090 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	113 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Miscellaneous

This chain was isolated from an IgG2a hybridoma protein that binds digoxin.

Ontologies

Keywords
Domain	Immunoglobulin V region Immunoglobulin domain
Molecular function	Monoclonal antibody
PTM	Disulfide bond
Technical term	3D-structure Complete proteome Direct protein sequencing Hybridoma Reference proteome
Gene Ontology (GO)
Molecular function	antigen binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – ›113

›113

Ig kappa chain V-II region 26-10

PRO_0000059776

Regions

Region

1 – 23

23

Framework-1

Region

24 – 39

16

Complementarity-determining-1

Region

40 – 54

15

Framework-2

Region

55 – 61

7

Complementarity-determining-2

Region

62 – 93

32

Framework-3

Region

94 – 102

9

Complementarity-determining-3

Region

103 – 112

10

Framework-4

Amino acid modifications

Disulfide bond

23 ↔ 93

By similarity

Experimental info

Non-terminal residue

113

1

Secondary structure

1

.

.

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113

Helix Strand Turn

Details...

Beta strand

4 – 7

4

Beta strand

9 – 13

5

Beta strand

19 – 27

9

Beta strand

38 – 43

6

Beta strand

50 – 54

5

Turn

55 – 57

3

Beta strand

67 – 71

5

Beta strand

73 – 82

10

Helix

85 – 87

3

Beta strand

89 – 95

7

Beta strand

97 – 100

4

Beta strand

107 – 111

5

Sequences

	Sequence		Length	Mass (Da)
	P01631 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: F9F39CE949A84C2A	FASTA	113	12,273
	10 20 30 40 50 60 DVVMTQTPLS LPVSLGDQAS ISCRSSQSLV HSNGNTYLNW YLQKAGQSPK LLIYKVSNRF 70 80 90 100 110 SGVPDRFSGS GSGTDFTLKI SRVEAEDLGI YFCSQTTHVP PTFGGGTKLE IKR

References

[1]	"Amino acid sequence of the light chain variable region from a mouse anti-digoxin hybridoma antibody." Novotny J., Margolies M.N. Biochemistry 22:1153-1158(1983) [PubMed: 6404298] [Abstract] Cited for: PROTEIN SEQUENCE. Strain: A/J.
+	Additional computationally mapped references.

Cross-references

Sequence databases

IPI

IPI00464378.

PIR

KVMS26. A01914.
A32248.
B32248.
C32248.
F32530.
H31485.
PH1030.
PH1031.
S53750.

UniGene

Mm.304143.
Mm.333124.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BFV	X-ray	2.10	L	5-113	[»]
1CFV	X-ray	2.10	L	5-113	[»]
1MAJ	NMR	-	A	1-113	[»]
1MAK	NMR	-	A	1-113	[»]
1SVZ	X-ray	1.89	A/B	4-112	[»]
2BFV	X-ray	2.50	L	5-113	[»]
2DLF	X-ray	1.55	L	1-113	[»]
2HKF	X-ray	2.01	L	1-113	[»]
2HKH	X-ray	2.10	L	1-113	[»]
2IPT	X-ray	2.00	L	1-113	[»]
2IQA	X-ray	2.00	A/L	1-113	[»]
2O5X	X-ray	2.05	L	1-113	[»]
2O5Y	X-ray	2.85	L	1-113	[»]
2O5Z	X-ray	2.40	L	1-113	[»]
2R0W	X-ray	2.50	L	1-113	[»]
2VQ1	X-ray	2.50	A/E	1-113	[»]

ProteinModelPortal

P01631.

SMR

P01631. Positions 1-113.

ModBase

Search...

Protein-protein interaction databases

IntAct

P01631. 1 interaction.

STRING

P01631.

Proteomic databases

PRIDE

P01631.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Phylogenomic databases

HOVERGEN

HBG018013.

Gene expression databases

Genevestigator

P01631.

Family and domain databases

InterPro

IPR007110. Ig-like.
IPR013783. Ig-like_fold.
IPR013106. Ig_V-set.
IPR003596. Ig_V-set_subgr.
[Graphical view]

Gene3D

G3DSA:2.60.40.10. Ig-like_fold. 1 hit.

Pfam

PF07686. V-set. 1 hit.
[Graphical view]

SMART

SM00406. IGv. 1 hit.
[Graphical view]

PROSITE

PS50835. IG_LIKE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

DrugBank

DB00693. Fluorescein.

Entry information

Entry name

KV2A7_MOUSE

Accession

Primary (citable) accession number: P01631

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	September 21, 2011
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program