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P01587 (CSF2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Granulocyte-macrophage colony-stimulating factor

Short name=GM-CSF
Alternative name(s):
Colony-stimulating factor
Short name=CSF
Gene names
Name:Csf2
Synonyms:Csfgm
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length141 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

Subunit structure

Monomer. The signaling GM-CSF receptor complex is a dodecamer of two head-to-head hexamers of two alpha, two beta, and two ligand subunits By similarity.

Subcellular location

Secreted.

Sequence similarities

Belongs to the GM-CSF family.

Sequence caution

The sequence CAA26192.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionCytokine
Growth factor
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processcellular response to lipopolysaccharide

Inferred from electronic annotation. Source: Ensembl

embryonic placenta development

Inferred from mutant phenotype PubMed 19228596. Source: MGI

epithelial fluid transport

Inferred from electronic annotation. Source: Ensembl

immune response

Inferred from electronic annotation. Source: InterPro

macrophage activation

Inferred from electronic annotation. Source: Ensembl

myeloid dendritic cell differentiation

Inferred from direct assay PubMed 12021780. Source: MGI

negative regulation of cytolysis

Inferred from electronic annotation. Source: Ensembl

negative regulation of extrinsic apoptotic signaling pathway in absence of ligand

Inferred from electronic annotation. Source: Ensembl

positive regulation of DNA replication

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell proliferation

Inferred from direct assay PubMed 10652277PubMed 1350248. Source: MGI

positive regulation of gene expression

Inferred from electronic annotation. Source: Ensembl

positive regulation of interleukin-23 production

Inferred from electronic annotation. Source: Ensembl

positive regulation of macrophage derived foam cell differentiation

Inferred from electronic annotation. Source: Ensembl

positive regulation of podosome assembly

Inferred from electronic annotation. Source: Ensembl

positive regulation of tyrosine phosphorylation of Stat5 protein

Inferred from electronic annotation. Source: Ensembl

regulation of cell proliferation

Inferred from genetic interaction PubMed 10678181. Source: MGI

regulation of gene expression

Inferred from direct assay PubMed 9697835. Source: MGI

   Cellular_componentextracellular space

Inferred from direct assay PubMed 21148126PubMed 7690439. Source: MGI

   Molecular_functioncytokine activity

Inferred from direct assay PubMed 10652277PubMed 1350248. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717
Chain18 – 141124Granulocyte-macrophage colony-stimulating factor
PRO_0000005866

Amino acid modifications

Glycosylation221O-linked (GalNAc...) By similarity
Glycosylation271O-linked (GalNAc...) By similarity
Glycosylation831N-linked (GlcNAc...)
Glycosylation921N-linked (GlcNAc...)
Disulfide bond68 ↔ 110 Ref.8
Disulfide bond102 ↔ 135 Ref.8

Experimental info

Mutagenesis381E → P: Reduction in bioactivity.
Mutagenesis731L → P: 25-fold reduction in bioactivity.
Mutagenesis771E → P: 50-fold reduction in bioactivity.
Mutagenesis801L → P: 450-fold reduction in bioactivity.
Mutagenesis1241L → P: 5500-fold reduction in bioactivity.
Sequence conflict251T → I in CAA29336. Ref.5
Sequence conflict251T → I AA sequence Ref.7
Sequence conflict1141V → A in AAA37483. Ref.6
Sequence conflict1391G → S in CAA29336. Ref.5
Sequence conflict1391G → V in CAA26820. Ref.2
Sequence conflict1391G → V no nucleotide entry Ref.3
Sequence conflict1391G → V in CAA26192. Ref.4
Sequence conflict1391G → V in CAA26193. Ref.4
Sequence conflict1391G → V in AAA37483. Ref.6

Sequences

Sequence LengthMass (Da)Tools
P01587 [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: 209C7CBB4FF77349

FASTA14116,091
        10         20         30         40         50         60 
MWLQNLLFLG IVVYSLSAPT RSPITVTRPW KHVEAIKEAL NLLDDMPVTL NEEVEVVSNE 

        70         80         90        100        110        120 
FSFKKLTCVQ TRLKIFEQGL RGNFTKLKGA LNMTASYYQT YCPPTPETDC ETQVTTYADF 

       130        140 
IDSLKTFLTD IPFECKKPGQ K 

« Hide

References

[1]"Structure of the chromosomal gene for granulocyte-macrophage colony stimulating factor: comparison of the mouse and human genes."
Miyatake S., Otsuka T., Yokota T., Lee F., Arai K.
EMBO J. 4:2561-2568(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The structure and expression of the murine gene encoding granulocyte-macrophage colony stimulating factor: evidence for utilisation of alternative promoters."
Stanley E.R., Metcalf D., Sobieszczuk P., Gough N.M., Dunn A.R.
EMBO J. 4:2569-2573(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Recombinant murine GM-CSF from E. coli has biological activity and is neutralized by a specific antiserum."
Delamarter J.F., Mermod J.-J., Liang C.M., Eliason J.F., Thatcher D.R.
EMBO J. 4:2575-2581(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"Structure and expression of the mRNA for murine granulocyte-macrophage colony stimulating factor."
Gough N.M., Metcalf D., Gough J., Grail D., Dunn A.R.
EMBO J. 4:645-653(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
[5]"Molecular cloning of cDNA encoding a murine haematopoietic growth regulator, granulocyte-macrophage colony stimulating factor."
Gough N.M., Gough J., Metcalf D., Kelso A., Grail D., Nicola N.A., Burgess A.W., Dunn A.R.
Nature 309:763-767(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 24-141.
Tissue: Lung.
[6]"Cloning, sequence, and expression of a human granulocyte/macrophage colony-stimulating factor."
Cantrell M.A., Anderson D., Cerretti D.P., Price V., McKereghan K., Tushinski R.J., Mochizuki D.Y., Larsen A., Grabstein S., Gillis S., Cosman D.
Proc. Natl. Acad. Sci. U.S.A. 82:6250-6254(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 18-141.
[7]"Purification and partial amino acid sequence of asialo murine granulocyte-macrophage colony stimulating factor."
Sparrow L.G., Metcalf D., Hunkapiller M.W., Hood L.E., Burgess A.W.
Proc. Natl. Acad. Sci. U.S.A. 82:292-296(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 24-57.
[8]"Characterization of human and mouse granulocyte-macrophage-colony-stimulating factors derived from Escherichia coli."
Schrimser J.L., Rose K., Simona M.G., Wingfield P.
Biochem. J. 247:195-199(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: DISULFIDE BONDS.
[9]"Single proline substitutions in predicted alpha-helices of murine granulocyte-macrophage colony-stimulating factor result in a loss in bioactivity and altered glycosylation."
Altmann S.W., Johnson G.D., Prystowsky M.B.
J. Biol. Chem. 266:5333-5341(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X03020 Genomic DNA. Translation: CAA26821.1.
X03019 mRNA. Translation: CAA26820.1.
X02333 mRNA. Translation: CAA26193.1.
X02333 mRNA. Translation: CAA26192.1. Different initiation.
X05906 mRNA. Translation: CAA29336.1.
M11848 mRNA. Translation: AAA37483.1.
PIRFQMSGM. I48368.
RefSeqNP_034099.2. NM_009969.4.
UniGeneMm.4922.

3D structure databases

ProteinModelPortalP01587.
SMRP01587. Positions 21-138.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteP01587.

Proteomic databases

PRIDEP01587.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000019060; ENSMUSP00000019060; ENSMUSG00000018916.
GeneID12981.
KEGGmmu:12981.
UCSCuc007ixm.1. mouse.

Organism-specific databases

CTD1437.
MGIMGI:1339752. Csf2.

Phylogenomic databases

eggNOGNOG42131.
HOGENOMHOG000037940.
HOVERGENHBG000532.
InParanoidP01587.
KOK05427.
OMATCLQTRL.
OrthoDBEOG7J9VRQ.
PhylomeDBP01587.
TreeFamTF338611.

Gene expression databases

ArrayExpressP01587.
BgeeP01587.
GenevestigatorP01587.

Family and domain databases

Gene3D1.20.1250.10. 1 hit.
InterProIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR000773. GM_colony-stim-fac.
[Graphical view]
PANTHERPTHR10059. PTHR10059. 1 hit.
PfamPF01109. GM_CSF. 1 hit.
[Graphical view]
PRINTSPR00693. GMCSFACTOR.
ProDomPD007349. GM_colony-stim-fac. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00040. CSF2. 1 hit.
[Graphical view]
SUPFAMSSF47266. SSF47266. 1 hit.
PROSITEPS00702. GM_CSF. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCSF2. mouse.
NextBio282756.
PROP01587.
SOURCESearch...

Entry information

Entry nameCSF2_MOUSE
AccessionPrimary (citable) accession number: P01587
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: April 1, 1988
Last modified: April 16, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot