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P01587

- CSF2_MOUSE

UniProt

P01587 - CSF2_MOUSE

Protein

Granulocyte-macrophage colony-stimulating factor

Gene

Csf2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

    GO - Molecular functioni

    1. cytokine activity Source: MGI

    GO - Biological processi

    1. cellular response to lipopolysaccharide Source: Ensembl
    2. embryonic placenta development Source: MGI
    3. epithelial fluid transport Source: Ensembl
    4. immune response Source: InterPro
    5. macrophage activation Source: Ensembl
    6. myeloid dendritic cell differentiation Source: MGI
    7. negative regulation of cytolysis Source: Ensembl
    8. negative regulation of extrinsic apoptotic signaling pathway in absence of ligand Source: Ensembl
    9. positive regulation of cell proliferation Source: MGI
    10. positive regulation of DNA replication Source: Ensembl
    11. positive regulation of gene expression Source: Ensembl
    12. positive regulation of interleukin-23 production Source: Ensembl
    13. positive regulation of macrophage derived foam cell differentiation Source: Ensembl
    14. positive regulation of podosome assembly Source: Ensembl
    15. positive regulation of tyrosine phosphorylation of Stat5 protein Source: Ensembl
    16. regulation of cell proliferation Source: MGI
    17. regulation of gene expression Source: MGI

    Keywords - Molecular functioni

    Cytokine, Growth factor

    Enzyme and pathway databases

    ReactomeiREACT_210793. Interleukin receptor SHC signaling.
    REACT_225836. Interleukin-3, 5 and GM-CSF signaling.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Granulocyte-macrophage colony-stimulating factor
    Short name:
    GM-CSF
    Alternative name(s):
    Colony-stimulating factor
    Short name:
    CSF
    Gene namesi
    Name:Csf2
    Synonyms:Csfgm
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:1339752. Csf2.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: MGI

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi38 – 381E → P: Reduction in bioactivity. 1 Publication
    Mutagenesisi73 – 731L → P: 25-fold reduction in bioactivity. 1 Publication
    Mutagenesisi77 – 771E → P: 50-fold reduction in bioactivity. 1 Publication
    Mutagenesisi80 – 801L → P: 450-fold reduction in bioactivity. 1 Publication
    Mutagenesisi124 – 1241L → P: 5500-fold reduction in bioactivity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1717Add
    BLAST
    Chaini18 – 141124Granulocyte-macrophage colony-stimulating factorPRO_0000005866Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi22 – 221O-linked (GalNAc...)By similarity
    Glycosylationi27 – 271O-linked (GalNAc...)By similarity
    Disulfide bondi68 ↔ 1101 Publication
    Glycosylationi83 – 831N-linked (GlcNAc...)
    Glycosylationi92 – 921N-linked (GlcNAc...)
    Disulfide bondi102 ↔ 1351 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiP01587.

    PTM databases

    PhosphoSiteiP01587.

    Expressioni

    Gene expression databases

    ArrayExpressiP01587.
    BgeeiP01587.
    GenevestigatoriP01587.

    Interactioni

    Subunit structurei

    Monomer. The signaling GM-CSF receptor complex is a dodecamer of two head-to-head hexamers of two alpha, two beta, and two ligand subunits By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP01587.
    SMRiP01587. Positions 21-138.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GM-CSF family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG42131.
    HOGENOMiHOG000037940.
    HOVERGENiHBG000532.
    InParanoidiP01587.
    KOiK05427.
    OMAiTCLQTRL.
    OrthoDBiEOG7J9VRQ.
    PhylomeDBiP01587.
    TreeFamiTF338611.

    Family and domain databases

    Gene3Di1.20.1250.10. 1 hit.
    InterProiIPR009079. 4_helix_cytokine-like_core.
    IPR012351. 4_helix_cytokine_core.
    IPR000773. GM_colony-stim-fac.
    [Graphical view]
    PANTHERiPTHR10059. PTHR10059. 1 hit.
    PfamiPF01109. GM_CSF. 1 hit.
    [Graphical view]
    PRINTSiPR00693. GMCSFACTOR.
    ProDomiPD007349. GM_colony-stim-fac. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00040. CSF2. 1 hit.
    [Graphical view]
    SUPFAMiSSF47266. SSF47266. 1 hit.
    PROSITEiPS00702. GM_CSF. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P01587-1 [UniParc]FASTAAdd to Basket

    « Hide

    MWLQNLLFLG IVVYSLSAPT RSPITVTRPW KHVEAIKEAL NLLDDMPVTL    50
    NEEVEVVSNE FSFKKLTCVQ TRLKIFEQGL RGNFTKLKGA LNMTASYYQT 100
    YCPPTPETDC ETQVTTYADF IDSLKTFLTD IPFECKKPGQ K 141
    Length:141
    Mass (Da):16,091
    Last modified:April 1, 1988 - v1
    Checksum:i209C7CBB4FF77349
    GO

    Sequence cautioni

    The sequence CAA26192.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti25 – 251T → I in CAA29336. (PubMed:6610831)Curated
    Sequence conflicti25 – 251T → I AA sequence (PubMed:3871523)Curated
    Sequence conflicti114 – 1141V → A in AAA37483. (PubMed:3898082)Curated
    Sequence conflicti139 – 1391G → S in CAA29336. (PubMed:6610831)Curated
    Sequence conflicti139 – 1391G → V in CAA26820. (PubMed:3876931)Curated
    Sequence conflicti139 – 1391G → V no nucleotide entry (PubMed:3902470)Curated
    Sequence conflicti139 – 1391G → V in CAA26192. (PubMed:3874057)Curated
    Sequence conflicti139 – 1391G → V in CAA26193. (PubMed:3874057)Curated
    Sequence conflicti139 – 1391G → V in AAA37483. (PubMed:3898082)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X03020 Genomic DNA. Translation: CAA26821.1.
    X03019 mRNA. Translation: CAA26820.1.
    X02333 mRNA. Translation: CAA26193.1.
    X02333 mRNA. Translation: CAA26192.1. Different initiation.
    X05906 mRNA. Translation: CAA29336.1.
    M11848 mRNA. Translation: AAA37483.1.
    CCDSiCCDS24692.1.
    PIRiI48368. FQMSGM.
    RefSeqiNP_034099.2. NM_009969.4.
    UniGeneiMm.4922.

    Genome annotation databases

    EnsembliENSMUST00000019060; ENSMUSP00000019060; ENSMUSG00000018916.
    GeneIDi12981.
    KEGGimmu:12981.
    UCSCiuc007ixm.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X03020 Genomic DNA. Translation: CAA26821.1 .
    X03019 mRNA. Translation: CAA26820.1 .
    X02333 mRNA. Translation: CAA26193.1 .
    X02333 mRNA. Translation: CAA26192.1 . Different initiation.
    X05906 mRNA. Translation: CAA29336.1 .
    M11848 mRNA. Translation: AAA37483.1 .
    CCDSi CCDS24692.1.
    PIRi I48368. FQMSGM.
    RefSeqi NP_034099.2. NM_009969.4.
    UniGenei Mm.4922.

    3D structure databases

    ProteinModelPortali P01587.
    SMRi P01587. Positions 21-138.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei P01587.

    Proteomic databases

    PRIDEi P01587.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000019060 ; ENSMUSP00000019060 ; ENSMUSG00000018916 .
    GeneIDi 12981.
    KEGGi mmu:12981.
    UCSCi uc007ixm.1. mouse.

    Organism-specific databases

    CTDi 1437.
    MGIi MGI:1339752. Csf2.

    Phylogenomic databases

    eggNOGi NOG42131.
    HOGENOMi HOG000037940.
    HOVERGENi HBG000532.
    InParanoidi P01587.
    KOi K05427.
    OMAi TCLQTRL.
    OrthoDBi EOG7J9VRQ.
    PhylomeDBi P01587.
    TreeFami TF338611.

    Enzyme and pathway databases

    Reactomei REACT_210793. Interleukin receptor SHC signaling.
    REACT_225836. Interleukin-3, 5 and GM-CSF signaling.

    Miscellaneous databases

    ChiTaRSi CSF2. mouse.
    NextBioi 282756.
    PROi P01587.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P01587.
    Bgeei P01587.
    Genevestigatori P01587.

    Family and domain databases

    Gene3Di 1.20.1250.10. 1 hit.
    InterProi IPR009079. 4_helix_cytokine-like_core.
    IPR012351. 4_helix_cytokine_core.
    IPR000773. GM_colony-stim-fac.
    [Graphical view ]
    PANTHERi PTHR10059. PTHR10059. 1 hit.
    Pfami PF01109. GM_CSF. 1 hit.
    [Graphical view ]
    PRINTSi PR00693. GMCSFACTOR.
    ProDomi PD007349. GM_colony-stim-fac. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM00040. CSF2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47266. SSF47266. 1 hit.
    PROSITEi PS00702. GM_CSF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure of the chromosomal gene for granulocyte-macrophage colony stimulating factor: comparison of the mouse and human genes."
      Miyatake S., Otsuka T., Yokota T., Lee F., Arai K.
      EMBO J. 4:2561-2568(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The structure and expression of the murine gene encoding granulocyte-macrophage colony stimulating factor: evidence for utilisation of alternative promoters."
      Stanley E.R., Metcalf D., Sobieszczuk P., Gough N.M., Dunn A.R.
      EMBO J. 4:2569-2573(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Recombinant murine GM-CSF from E. coli has biological activity and is neutralized by a specific antiserum."
      Delamarter J.F., Mermod J.-J., Liang C.M., Eliason J.F., Thatcher D.R.
      EMBO J. 4:2575-2581(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. "Structure and expression of the mRNA for murine granulocyte-macrophage colony stimulating factor."
      Gough N.M., Metcalf D., Gough J., Grail D., Dunn A.R.
      EMBO J. 4:645-653(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
    5. "Molecular cloning of cDNA encoding a murine haematopoietic growth regulator, granulocyte-macrophage colony stimulating factor."
      Gough N.M., Gough J., Metcalf D., Kelso A., Grail D., Nicola N.A., Burgess A.W., Dunn A.R.
      Nature 309:763-767(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 24-141.
      Tissue: Lung.
    6. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 18-141.
    7. "Purification and partial amino acid sequence of asialo murine granulocyte-macrophage colony stimulating factor."
      Sparrow L.G., Metcalf D., Hunkapiller M.W., Hood L.E., Burgess A.W.
      Proc. Natl. Acad. Sci. U.S.A. 82:292-296(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 24-57.
    8. "Characterization of human and mouse granulocyte-macrophage-colony-stimulating factors derived from Escherichia coli."
      Schrimser J.L., Rose K., Simona M.G., Wingfield P.
      Biochem. J. 247:195-199(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISULFIDE BONDS.
    9. "Single proline substitutions in predicted alpha-helices of murine granulocyte-macrophage colony-stimulating factor result in a loss in bioactivity and altered glycosylation."
      Altmann S.W., Johnson G.D., Prystowsky M.B.
      J. Biol. Chem. 266:5333-5341(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS.

    Entry informationi

    Entry nameiCSF2_MOUSE
    AccessioniPrimary (citable) accession number: P01587
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: April 1, 1988
    Last modified: October 1, 2014
    This is version 121 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3