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P01587

- CSF2_MOUSE

UniProt

P01587 - CSF2_MOUSE

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Protein

Granulocyte-macrophage colony-stimulating factor

Gene

Csf2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

GO - Molecular functioni

  1. cytokine activity Source: MGI

GO - Biological processi

  1. cellular response to lipopolysaccharide Source: Ensembl
  2. embryonic placenta development Source: MGI
  3. epithelial fluid transport Source: Ensembl
  4. immune response Source: InterPro
  5. macrophage activation Source: Ensembl
  6. myeloid dendritic cell differentiation Source: MGI
  7. negative regulation of cytolysis Source: Ensembl
  8. negative regulation of extrinsic apoptotic signaling pathway in absence of ligand Source: Ensembl
  9. positive regulation of cell proliferation Source: MGI
  10. positive regulation of DNA replication Source: Ensembl
  11. positive regulation of gene expression Source: Ensembl
  12. positive regulation of interleukin-23 production Source: Ensembl
  13. positive regulation of macrophage derived foam cell differentiation Source: Ensembl
  14. positive regulation of podosome assembly Source: Ensembl
  15. positive regulation of tyrosine phosphorylation of Stat5 protein Source: Ensembl
  16. regulation of cell proliferation Source: MGI
  17. regulation of gene expression Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Growth factor

Enzyme and pathway databases

ReactomeiREACT_210793. Interleukin receptor SHC signaling.
REACT_220092. GPVI-mediated activation cascade.
REACT_223974. G beta:gamma signalling through PI3Kgamma.
REACT_225836. Interleukin-3, 5 and GM-CSF signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Granulocyte-macrophage colony-stimulating factor
Short name:
GM-CSF
Alternative name(s):
Colony-stimulating factor
Short name:
CSF
Gene namesi
Name:Csf2
Synonyms:Csfgm
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:1339752. Csf2.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi38 – 381E → P: Reduction in bioactivity. 1 Publication
Mutagenesisi73 – 731L → P: 25-fold reduction in bioactivity. 1 Publication
Mutagenesisi77 – 771E → P: 50-fold reduction in bioactivity. 1 Publication
Mutagenesisi80 – 801L → P: 450-fold reduction in bioactivity. 1 Publication
Mutagenesisi124 – 1241L → P: 5500-fold reduction in bioactivity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717Add
BLAST
Chaini18 – 141124Granulocyte-macrophage colony-stimulating factorPRO_0000005866Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi22 – 221O-linked (GalNAc...)By similarity
Glycosylationi27 – 271O-linked (GalNAc...)By similarity
Disulfide bondi68 ↔ 1101 Publication
Glycosylationi83 – 831N-linked (GlcNAc...)
Glycosylationi92 – 921N-linked (GlcNAc...)
Disulfide bondi102 ↔ 1351 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP01587.

PTM databases

PhosphoSiteiP01587.

Expressioni

Gene expression databases

BgeeiP01587.
ExpressionAtlasiP01587. baseline and differential.
GenevestigatoriP01587.

Interactioni

Subunit structurei

Monomer. The signaling GM-CSF receptor complex is a dodecamer of two head-to-head hexamers of two alpha, two beta, and two ligand subunits (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP01587.
SMRiP01587. Positions 21-138.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GM-CSF family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG42131.
HOGENOMiHOG000037940.
HOVERGENiHBG000532.
InParanoidiP01587.
KOiK05427.
OMAiTCLQTRL.
OrthoDBiEOG7J9VRQ.
PhylomeDBiP01587.
TreeFamiTF338611.

Family and domain databases

Gene3Di1.20.1250.10. 1 hit.
InterProiIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR000773. GM_colony-stim-fac.
[Graphical view]
PANTHERiPTHR10059. PTHR10059. 1 hit.
PfamiPF01109. GM_CSF. 1 hit.
[Graphical view]
PRINTSiPR00693. GMCSFACTOR.
ProDomiPD007349. GM_colony-stim-fac. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00040. CSF2. 1 hit.
[Graphical view]
SUPFAMiSSF47266. SSF47266. 1 hit.
PROSITEiPS00702. GM_CSF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01587-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MWLQNLLFLG IVVYSLSAPT RSPITVTRPW KHVEAIKEAL NLLDDMPVTL
60 70 80 90 100
NEEVEVVSNE FSFKKLTCVQ TRLKIFEQGL RGNFTKLKGA LNMTASYYQT
110 120 130 140
YCPPTPETDC ETQVTTYADF IDSLKTFLTD IPFECKKPGQ K
Length:141
Mass (Da):16,091
Last modified:April 1, 1988 - v1
Checksum:i209C7CBB4FF77349
GO

Sequence cautioni

The sequence CAA26192.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti25 – 251T → I in CAA29336. (PubMed:6610831)Curated
Sequence conflicti25 – 251T → I AA sequence (PubMed:3871523)Curated
Sequence conflicti114 – 1141V → A in AAA37483. (PubMed:3898082)Curated
Sequence conflicti139 – 1391G → S in CAA29336. (PubMed:6610831)Curated
Sequence conflicti139 – 1391G → V in CAA26820. (PubMed:3876931)Curated
Sequence conflicti139 – 1391G → V no nucleotide entry (PubMed:3902470)Curated
Sequence conflicti139 – 1391G → V in CAA26192. (PubMed:3874057)Curated
Sequence conflicti139 – 1391G → V in CAA26193. (PubMed:3874057)Curated
Sequence conflicti139 – 1391G → V in AAA37483. (PubMed:3898082)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03020 Genomic DNA. Translation: CAA26821.1.
X03019 mRNA. Translation: CAA26820.1.
X02333 mRNA. Translation: CAA26193.1.
X02333 mRNA. Translation: CAA26192.1. Different initiation.
X05906 mRNA. Translation: CAA29336.1.
M11848 mRNA. Translation: AAA37483.1.
CCDSiCCDS24692.1.
PIRiI48368. FQMSGM.
RefSeqiNP_034099.2. NM_009969.4.
UniGeneiMm.4922.

Genome annotation databases

EnsembliENSMUST00000019060; ENSMUSP00000019060; ENSMUSG00000018916.
GeneIDi12981.
KEGGimmu:12981.
UCSCiuc007ixm.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03020 Genomic DNA. Translation: CAA26821.1 .
X03019 mRNA. Translation: CAA26820.1 .
X02333 mRNA. Translation: CAA26193.1 .
X02333 mRNA. Translation: CAA26192.1 . Different initiation.
X05906 mRNA. Translation: CAA29336.1 .
M11848 mRNA. Translation: AAA37483.1 .
CCDSi CCDS24692.1.
PIRi I48368. FQMSGM.
RefSeqi NP_034099.2. NM_009969.4.
UniGenei Mm.4922.

3D structure databases

ProteinModelPortali P01587.
SMRi P01587. Positions 21-138.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei P01587.

Proteomic databases

PRIDEi P01587.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000019060 ; ENSMUSP00000019060 ; ENSMUSG00000018916 .
GeneIDi 12981.
KEGGi mmu:12981.
UCSCi uc007ixm.1. mouse.

Organism-specific databases

CTDi 1437.
MGIi MGI:1339752. Csf2.

Phylogenomic databases

eggNOGi NOG42131.
HOGENOMi HOG000037940.
HOVERGENi HBG000532.
InParanoidi P01587.
KOi K05427.
OMAi TCLQTRL.
OrthoDBi EOG7J9VRQ.
PhylomeDBi P01587.
TreeFami TF338611.

Enzyme and pathway databases

Reactomei REACT_210793. Interleukin receptor SHC signaling.
REACT_220092. GPVI-mediated activation cascade.
REACT_223974. G beta:gamma signalling through PI3Kgamma.
REACT_225836. Interleukin-3, 5 and GM-CSF signaling.

Miscellaneous databases

ChiTaRSi Csf2. mouse.
NextBioi 282756.
PROi P01587.
SOURCEi Search...

Gene expression databases

Bgeei P01587.
ExpressionAtlasi P01587. baseline and differential.
Genevestigatori P01587.

Family and domain databases

Gene3Di 1.20.1250.10. 1 hit.
InterProi IPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR000773. GM_colony-stim-fac.
[Graphical view ]
PANTHERi PTHR10059. PTHR10059. 1 hit.
Pfami PF01109. GM_CSF. 1 hit.
[Graphical view ]
PRINTSi PR00693. GMCSFACTOR.
ProDomi PD007349. GM_colony-stim-fac. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SMARTi SM00040. CSF2. 1 hit.
[Graphical view ]
SUPFAMi SSF47266. SSF47266. 1 hit.
PROSITEi PS00702. GM_CSF. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Structure of the chromosomal gene for granulocyte-macrophage colony stimulating factor: comparison of the mouse and human genes."
    Miyatake S., Otsuka T., Yokota T., Lee F., Arai K.
    EMBO J. 4:2561-2568(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The structure and expression of the murine gene encoding granulocyte-macrophage colony stimulating factor: evidence for utilisation of alternative promoters."
    Stanley E.R., Metcalf D., Sobieszczuk P., Gough N.M., Dunn A.R.
    EMBO J. 4:2569-2573(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Recombinant murine GM-CSF from E. coli has biological activity and is neutralized by a specific antiserum."
    Delamarter J.F., Mermod J.-J., Liang C.M., Eliason J.F., Thatcher D.R.
    EMBO J. 4:2575-2581(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "Structure and expression of the mRNA for murine granulocyte-macrophage colony stimulating factor."
    Gough N.M., Metcalf D., Gough J., Grail D., Dunn A.R.
    EMBO J. 4:645-653(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  5. "Molecular cloning of cDNA encoding a murine haematopoietic growth regulator, granulocyte-macrophage colony stimulating factor."
    Gough N.M., Gough J., Metcalf D., Kelso A., Grail D., Nicola N.A., Burgess A.W., Dunn A.R.
    Nature 309:763-767(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 24-141.
    Tissue: Lung.
  6. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 18-141.
  7. "Purification and partial amino acid sequence of asialo murine granulocyte-macrophage colony stimulating factor."
    Sparrow L.G., Metcalf D., Hunkapiller M.W., Hood L.E., Burgess A.W.
    Proc. Natl. Acad. Sci. U.S.A. 82:292-296(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 24-57.
  8. "Characterization of human and mouse granulocyte-macrophage-colony-stimulating factors derived from Escherichia coli."
    Schrimser J.L., Rose K., Simona M.G., Wingfield P.
    Biochem. J. 247:195-199(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISULFIDE BONDS.
  9. "Single proline substitutions in predicted alpha-helices of murine granulocyte-macrophage colony-stimulating factor result in a loss in bioactivity and altered glycosylation."
    Altmann S.W., Johnson G.D., Prystowsky M.B.
    J. Biol. Chem. 266:5333-5341(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS.

Entry informationi

Entry nameiCSF2_MOUSE
AccessioniPrimary (citable) accession number: P01587
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: April 1, 1988
Last modified: November 26, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3