SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P01586

- IL3_MOUSE

UniProt

P01586 - IL3_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Interleukin-3
Gene
Il3, Csfmu, Il-3
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.
This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.

GO - Molecular functioni

  1. cytokine activity Source: MGI
  2. growth factor activity Source: MGI

GO - Biological processi

  1. cytokine-mediated signaling pathway Source: MGI
  2. extrinsic apoptotic signaling pathway in absence of ligand Source: MGI
  3. immune response Source: InterPro
  4. inflammatory response Source: MGI
  5. myeloid cell apoptotic process Source: MGI
  6. negative regulation of apoptotic process Source: MGI
  7. negative regulation of autophagy Source: MGI
  8. negative regulation of mast cell apoptotic process Source: MGI
  9. positive regulation of JNK cascade Source: MGI
  10. positive regulation of cell proliferation Source: MGI
  11. positive regulation of mast cell proliferation Source: MGI
  12. positive regulation of myeloid leukocyte differentiation Source: MGI
  13. positive regulation of peptidyl-tyrosine phosphorylation Source: MGI
  14. positive regulation of protein phosphorylation Source: MGI
  15. regulation of gene expression Source: MGI
  16. regulation of glycolytic process Source: MGI
  17. regulation of protein dephosphorylation Source: MGI
  18. response to hypoxia Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Growth factor

Names & Taxonomyi

Protein namesi
Recommended name:
Interleukin-3
Short name:
IL-3
Alternative name(s):
Hematopoietic growth factor
Mast cell growth factor
Short name:
MCGF
Multipotential colony-stimulating factor
P-cell-stimulating factor
Gene namesi
Name:Il3
Synonyms:Csfmu, Il-3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:96552. Il3.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626
Add
BLAST
Chaini27 – 166140Interleukin-3
PRO_0000015519Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi42 – 421N-linked (GlcNAc...)1 Publication
Disulfide bondi43 ↔ 1062 Publications
Disulfide bondi105 ↔ 1662 Publications
Glycosylationi112 – 1121N-linked (GlcNAc...); partial1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP01586.

PTM databases

PhosphoSiteiP01586.

Expressioni

Tissue specificityi

Activated T-cells, mast cells, natural killer cells.

Gene expression databases

ArrayExpressiP01586.
BgeeiP01586.
CleanExiMM_IL3.
GenevestigatoriP01586.

Interactioni

Subunit structurei

Monomer.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi37 – 415
Helixi44 – 5310
Helixi67 – 693
Turni71 – 733
Helixi74 – 8512
Turni86 – 883
Helixi90 – 934
Helixi97 – 10610
Helixi124 – 13714
Beta strandi140 – 1423
Beta strandi151 – 1533

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2L3ONMR-A33-156[»]
ProteinModelPortaliP01586.
SMRiP01586. Positions 33-156.

Family & Domainsi

Sequence similaritiesi

Belongs to the IL-3 family.

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiopiNOG27251.
HOGENOMiHOG000113051.
HOVERGENiHBG006148.
InParanoidiP01586.
KOiK04736.
OMAiNDSALPG.
OrthoDBiEOG7BZVW6.
PhylomeDBiP01586.
TreeFamiTF338567.

Family and domain databases

Gene3Di1.20.1250.10. 1 hit.
InterProiIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR002183. IL-3.
[Graphical view]
PANTHERiPTHR10079. PTHR10079. 1 hit.
PfamiPF02059. IL3. 1 hit.
[Graphical view]
PIRSFiPIRSF001939. IL-3. 1 hit.
PRINTSiPR00430. INTERLEUKIN3.
SUPFAMiSSF47266. SSF47266. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01586-1 [UniParc]FASTAAdd to Basket

« Hide

MVLASSTTSI HTMLLLLLML FHLGLQASIS GRDTHRLTRT LNCSSIVKEI    50
IGKLPEPELK TDDEGPSLRN KSFRRVNLSK FVESQGEVDP EDRYVIKSNL 100
QKLNCCLPTS ANDSALPGVF IRDLDDFRKK LRFYMVHLND LETVLTSRPP 150
QPASGSVSPN RGTVEC 166
Length:166
Mass (Da):18,540
Last modified:July 21, 1986 - v1
Checksum:iF38EE7A9E361CAA2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K01850 mRNA. Translation: AAA39291.1.
K01668 mRNA. Translation: AAA39507.1.
K03233 Genomic DNA. Translation: AAA39293.1.
X02732 Genomic DNA. Translation: CAA26514.1.
M20128, M14394 Genomic DNA. Translation: AAA39308.1.
CCDSiCCDS24693.1.
PIRiA25481. ICMS3.
RefSeqiNP_034686.2. NM_010556.4.
UniGeneiMm.983.

Genome annotation databases

EnsembliENSMUST00000019058; ENSMUSP00000019058; ENSMUSG00000018914.
GeneIDi16187.
KEGGimmu:16187.
UCSCiuc007ixn.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K01850 mRNA. Translation: AAA39291.1 .
K01668 mRNA. Translation: AAA39507.1 .
K03233 Genomic DNA. Translation: AAA39293.1 .
X02732 Genomic DNA. Translation: CAA26514.1 .
M20128 , M14394 Genomic DNA. Translation: AAA39308.1 .
CCDSi CCDS24693.1.
PIRi A25481. ICMS3.
RefSeqi NP_034686.2. NM_010556.4.
UniGenei Mm.983.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2L3O NMR - A 33-156 [» ]
ProteinModelPortali P01586.
SMRi P01586. Positions 33-156.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei P01586.

Proteomic databases

PRIDEi P01586.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000019058 ; ENSMUSP00000019058 ; ENSMUSG00000018914 .
GeneIDi 16187.
KEGGi mmu:16187.
UCSCi uc007ixn.1. mouse.

Organism-specific databases

CTDi 3562.
MGIi MGI:96552. Il3.

Phylogenomic databases

eggNOGi opiNOG27251.
HOGENOMi HOG000113051.
HOVERGENi HBG006148.
InParanoidi P01586.
KOi K04736.
OMAi NDSALPG.
OrthoDBi EOG7BZVW6.
PhylomeDBi P01586.
TreeFami TF338567.

Miscellaneous databases

NextBioi 289085.
PROi P01586.
SOURCEi Search...

Gene expression databases

ArrayExpressi P01586.
Bgeei P01586.
CleanExi MM_IL3.
Genevestigatori P01586.

Family and domain databases

Gene3Di 1.20.1250.10. 1 hit.
InterProi IPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR002183. IL-3.
[Graphical view ]
PANTHERi PTHR10079. PTHR10079. 1 hit.
Pfami PF02059. IL3. 1 hit.
[Graphical view ]
PIRSFi PIRSF001939. IL-3. 1 hit.
PRINTSi PR00430. INTERLEUKIN3.
SUPFAMi SSF47266. SSF47266. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Isolation and characterization of a mouse cDNA clone that expresses mast-cell growth-factor activity in monkey cells."
    Yokota T., Lee F., Rennick D., Hall C., Arai N., Mosmann T., Nabel G., Cantor H., Arai K.
    Proc. Natl. Acad. Sci. U.S.A. 81:1070-1074(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Structure of the chromosomal gene for murine interleukin 3."
    Miyatake S., Yokota T., Lee F., Arai K.
    Proc. Natl. Acad. Sci. U.S.A. 82:316-320(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Cloning and nucleotide sequence of the murine interleukin-3 gene."
    Campbell H.D., Ymer S., Fung M.-C., Young I.G.
    Eur. J. Biochem. 150:297-304(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. "Isolation and characterization of a genomic DDD mouse interleukin-3 gene."
    Todokoro K., Yamamoto A., Amanuma H., Ikawa Y.
    Gene 39:103-107(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DDD.
  6. "Determination of the glycosylation patterns, disulfide linkages, and protein heterogeneities of baculovirus-expressed mouse interleukin-3 by mass spectrometry."
    Knepper T.P., Arbogast B., Schreurs J., Deinzer M.L.
    Biochemistry 31:11651-11659(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION AT ASN-42 AND ASN-112, DISULFIDE BONDS.
  7. "Murine interleukin-3: structure, dynamics, and conformational heterogeneity in solution."
    Yao S., Young I.G., Norton R.S., Murphy J.M.
    Biochemistry 50:2464-2477(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 33-156, DISULFIDE BOND.

Entry informationi

Entry nameiIL3_MOUSE
AccessioniPrimary (citable) accession number: P01586
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: July 9, 2014
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi