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P01586 (IL3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 126. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Interleukin-3

Short name=IL-3
Alternative name(s):
Hematopoietic growth factor
Mast cell growth factor
Short name=MCGF
Multipotential colony-stimulating factor
P-cell-stimulating factor
Gene names
Name:Il3
Synonyms:Csfmu, Il-3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length166 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.

This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.

Subunit structure

Monomer.

Subcellular location

Secreted.

Tissue specificity

Activated T-cells, mast cells, natural killer cells.

Sequence similarities

Belongs to the IL-3 family.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionCytokine
Growth factor
   PTMDisulfide bond
Glycoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcytokine-mediated signaling pathway

Inferred from direct assay PubMed 7519779. Source: MGI

extrinsic apoptotic signaling pathway in absence of ligand

Inferred from direct assay PubMed 11527400PubMed 12124386PubMed 12944463PubMed 14967141PubMed 15231831PubMed 15680329. Source: MGI

immune response

Inferred from electronic annotation. Source: InterPro

inflammatory response

Inferred from mutant phenotype PubMed 15647285. Source: MGI

myeloid cell apoptotic process

Inferred from direct assay PubMed 9202146. Source: MGI

negative regulation of apoptotic process

Inferred from direct assay PubMed 10652277PubMed 7736574. Source: MGI

negative regulation of autophagy

Inferred from direct assay PubMed 15680329. Source: MGI

negative regulation of mast cell apoptotic process

Inferred from direct assay PubMed 12714518. Source: MGI

positive regulation of JNK cascade

Inferred from direct assay PubMed 14967141. Source: MGI

positive regulation of cell proliferation

Inferred from direct assay PubMed 1350248PubMed 7736574PubMed 7957045. Source: MGI

positive regulation of mast cell proliferation

Inferred from direct assay PubMed 12714518PubMed 15947484. Source: MGI

positive regulation of myeloid leukocyte differentiation

Inferred from genetic interaction PubMed 1375116PubMed 7690439. Source: MGI

positive regulation of peptidyl-tyrosine phosphorylation

Inferred from direct assay PubMed 10872802PubMed 7519779. Source: MGI

positive regulation of protein phosphorylation

Inferred from direct assay PubMed 14967141. Source: MGI

regulation of gene expression

Inferred from direct assay PubMed 9697835. Source: MGI

regulation of glycolytic process

Inferred from genetic interaction PubMed 17437992. Source: MGI

regulation of protein dephosphorylation

Inferred from direct assay PubMed 12944463. Source: MGI

response to hypoxia

Inferred from genetic interaction PubMed 17437992. Source: MGI

   Cellular_componentextracellular space

Inferred from direct assay PubMed 7690439. Source: MGI

   Molecular_functioncytokine activity

Inferred from direct assay PubMed 10652277PubMed 10872802PubMed 12714518PubMed 1350248PubMed 14967141PubMed 7519779PubMed 7736574PubMed 7957045. Source: MGI

growth factor activity

Inferred from direct assay PubMed 12714518PubMed 7957045. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626
Chain27 – 166140Interleukin-3
PRO_0000015519

Amino acid modifications

Glycosylation421N-linked (GlcNAc...) Ref.6
Glycosylation1121N-linked (GlcNAc...); partial Ref.6
Disulfide bond43 ↔ 106 Ref.6 Ref.7
Disulfide bond105 ↔ 166 Ref.6 Ref.7

Secondary structure

..................... 166
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P01586 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: F38EE7A9E361CAA2

FASTA16618,540
        10         20         30         40         50         60 
MVLASSTTSI HTMLLLLLML FHLGLQASIS GRDTHRLTRT LNCSSIVKEI IGKLPEPELK 

        70         80         90        100        110        120 
TDDEGPSLRN KSFRRVNLSK FVESQGEVDP EDRYVIKSNL QKLNCCLPTS ANDSALPGVF 

       130        140        150        160 
IRDLDDFRKK LRFYMVHLND LETVLTSRPP QPASGSVSPN RGTVEC 

« Hide

References

[1]"Molecular cloning of cDNA for murine interleukin-3."
Fung M.-C., Hapel A.J., Ymer S., Cohen D.R., Johnson R.M., Campbell H.D., Young I.G.
Nature 307:233-237(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Isolation and characterization of a mouse cDNA clone that expresses mast-cell growth-factor activity in monkey cells."
Yokota T., Lee F., Rennick D., Hall C., Arai N., Mosmann T., Nabel G., Cantor H., Arai K.
Proc. Natl. Acad. Sci. U.S.A. 81:1070-1074(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Structure of the chromosomal gene for murine interleukin 3."
Miyatake S., Yokota T., Lee F., Arai K.
Proc. Natl. Acad. Sci. U.S.A. 82:316-320(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Cloning and nucleotide sequence of the murine interleukin-3 gene."
Campbell H.D., Ymer S., Fung M.-C., Young I.G.
Eur. J. Biochem. 150:297-304(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"Isolation and characterization of a genomic DDD mouse interleukin-3 gene."
Todokoro K., Yamamoto A., Amanuma H., Ikawa Y.
Gene 39:103-107(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: DDD.
[6]"Determination of the glycosylation patterns, disulfide linkages, and protein heterogeneities of baculovirus-expressed mouse interleukin-3 by mass spectrometry."
Knepper T.P., Arbogast B., Schreurs J., Deinzer M.L.
Biochemistry 31:11651-11659(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION AT ASN-42 AND ASN-112, DISULFIDE BONDS.
[7]"Murine interleukin-3: structure, dynamics, and conformational heterogeneity in solution."
Yao S., Young I.G., Norton R.S., Murphy J.M.
Biochemistry 50:2464-2477(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 33-156, DISULFIDE BOND.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
K01850 mRNA. Translation: AAA39291.1.
K01668 mRNA. Translation: AAA39507.1.
K03233 Genomic DNA. Translation: AAA39293.1.
X02732 Genomic DNA. Translation: CAA26514.1.
M20128, M14394 Genomic DNA. Translation: AAA39308.1.
CCDSCCDS24693.1.
PIRICMS3. A25481.
RefSeqNP_034686.2. NM_010556.4.
UniGeneMm.983.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2L3ONMR-A33-156[»]
ProteinModelPortalP01586.
SMRP01586. Positions 33-156.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteP01586.

Proteomic databases

PRIDEP01586.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000019058; ENSMUSP00000019058; ENSMUSG00000018914.
GeneID16187.
KEGGmmu:16187.
UCSCuc007ixn.1. mouse.

Organism-specific databases

CTD3562.
MGIMGI:96552. Il3.

Phylogenomic databases

eggNOGopiNOG27251.
HOGENOMHOG000113051.
HOVERGENHBG006148.
InParanoidP01586.
KOK04736.
OMANDSALPG.
OrthoDBEOG7BZVW6.
PhylomeDBP01586.
TreeFamTF338567.

Gene expression databases

ArrayExpressP01586.
BgeeP01586.
CleanExMM_IL3.
GenevestigatorP01586.

Family and domain databases

Gene3D1.20.1250.10. 1 hit.
InterProIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR002183. IL-3.
[Graphical view]
PANTHERPTHR10079. PTHR10079. 1 hit.
PfamPF02059. IL3. 1 hit.
[Graphical view]
PIRSFPIRSF001939. IL-3. 1 hit.
PRINTSPR00430. INTERLEUKIN3.
SUPFAMSSF47266. SSF47266. 1 hit.
ProtoNetSearch...

Other

NextBio289085.
PROP01586.
SOURCESearch...

Entry information

Entry nameIL3_MOUSE
AccessionPrimary (citable) accession number: P01586
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: July 9, 2014
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot