Interleukin-3 precursor - Mus musculus (Mouse)

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P01586 (IL3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 116. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Interleukin-3
Short name=IL-3

Alternative name(s):
Hematopoietic growth factor
Mast cell growth factor
Short name=MCGF
Multipotential colony-stimulating factor
P-cell-stimulating factor

Gene names

Name:	Il3
Synonyms:	Csfmu, Il-3

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	166 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.
Subunit structure	Monomer.
Subcellular location	Secreted.
Tissue specificity	Activated T-cells, mast cells, natural killer cells.
Sequence similarities	Belongs to the IL-3 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Cytokine Growth factor
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cytokine-mediated signaling pathway Inferred from direct assay PubMed 7519779. Source: MGI immune response Inferred from electronic annotation. Source: InterPro myeloid cell apoptotic process Inferred from direct assay PubMed 9202146. Source: MGI negative regulation of autophagy Inferred from direct assay PubMed 15680329. Source: MGI negative regulation of mast cell apoptotic process Inferred from direct assay PubMed 12714518. Source: MGI positive regulation of JNK cascade Inferred from direct assay PubMed 14967141. Source: MGI positive regulation of mast cell proliferation Inferred from direct assay PubMed 12714518 PubMed 15947484. Source: MGI positive regulation of myeloid leukocyte differentiation Inferred from genetic interaction PubMed 1375116 PubMed 7690439. Source: MGI positive regulation of peptidyl-tyrosine phosphorylation Inferred from direct assay PubMed 10872802 PubMed 7519779. Source: MGI regulation of gene expression Inferred from direct assay PubMed 9697835. Source: MGI regulation of glycolysis Inferred from genetic interaction PubMed 17437992. Source: MGI regulation of protein dephosphorylation Inferred from direct assay PubMed 12944463. Source: MGI response to hypoxia Inferred from genetic interaction PubMed 17437992. Source: MGI
Cellular_component	extracellular space Inferred from direct assay PubMed 7690439. Source: MGI
Molecular_function	cytokine activity Inferred from direct assay PubMed 10652277 PubMed 10872802 PubMed 12714518 PubMed 1350248 PubMed 14967141 PubMed 7519779 PubMed 7736574 PubMed 7957045. Source: MGI growth factor activity Inferred from direct assay PubMed 12714518 PubMed 7957045. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 26

Chain

27 – 166

140

Interleukin-3

PRO_0000015519

Amino acid modifications

Glycosylation

N-linked (GlcNAc...) Ref.6

Glycosylation

112

N-linked (GlcNAc...); partial Ref.6

Disulfide bond

Disulfide bond

Secondary structure

166

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P01586 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: F38EE7A9E361CAA2
Show »

FASTA

166

18,540

        10         20         30         40         50         60 
MVLASSTTSI HTMLLLLLML FHLGLQASIS GRDTHRLTRT LNCSSIVKEI IGKLPEPELK 

        70         80         90        100        110        120 
TDDEGPSLRN KSFRRVNLSK FVESQGEVDP EDRYVIKSNL QKLNCCLPTS ANDSALPGVF 

       130        140        150        160 
IRDLDDFRKK LRFYMVHLND LETVLTSRPP QPASGSVSPN RGTVEC

« Hide

References

[1]	"Molecular cloning of cDNA for murine interleukin-3." Fung M.-C., Hapel A.J., Ymer S., Cohen D.R., Johnson R.M., Campbell H.D., Young I.G. Nature 307:233-237(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Isolation and characterization of a mouse cDNA clone that expresses mast-cell growth-factor activity in monkey cells." Yokota T., Lee F., Rennick D., Hall C., Arai N., Mosmann T., Nabel G., Cantor H., Arai K. Proc. Natl. Acad. Sci. U.S.A. 81:1070-1074(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Structure of the chromosomal gene for murine interleukin 3." Miyatake S., Yokota T., Lee F., Arai K. Proc. Natl. Acad. Sci. U.S.A. 82:316-320(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Cloning and nucleotide sequence of the murine interleukin-3 gene." Campbell H.D., Ymer S., Fung M.-C., Young I.G. Eur. J. Biochem. 150:297-304(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"Isolation and characterization of a genomic DDD mouse interleukin-3 gene." Todokoro K., Yamamoto A., Amanuma H., Ikawa Y. Gene 39:103-107(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DDD.
[6]	"Determination of the glycosylation patterns, disulfide linkages, and protein heterogeneities of baculovirus-expressed mouse interleukin-3 by mass spectrometry." Knepper T.P., Arbogast B., Schreurs J., Deinzer M.L. Biochemistry 31:11651-11659(1992) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION AT ASN-42 AND ASN-112, DISULFIDE BONDS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

K01850 mRNA. Translation: AAA39291.1.
K01668 mRNA. Translation: AAA39507.1.
K03233 Genomic DNA. Translation: AAA39293.1.
X02732 Genomic DNA. Translation: CAA26514.1.
M20128, M14394 Genomic DNA. Translation: AAA39308.1.

IPI

IPI00323906.

PIR

ICMS3. A25481.

RefSeq

NP_034686.2. NM_010556.4.

UniGene

Mm.983.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2L3O	NMR	-	A	33-156	[»]

ProteinModelPortal

P01586.

SMR

P01586. Positions 33-156.

ModBase

Search...

PTM databases

PhosphoSite

P01586.

Proteomic databases

PRIDE

P01586.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000019058; ENSMUSP00000019058; ENSMUSG00000018914.

GeneID

16187.

KEGG

mmu:16187.

Organism-specific databases

CTD

3562.

MGI

MGI:96552. Il3.

Phylogenomic databases

eggNOG

opiNOG27251.

HOGENOM

HOG000113051.

HOVERGEN

HBG006148.

InParanoid

P01586.

K04736.

OMA

NDSALPG.

OrthoDB

EOG4QNMXW.

Gene expression databases

ArrayExpress

P01586.

Bgee

P01586.

CleanEx

MM_IL3.

Genevestigator

P01586.

GermOnline

ENSMUSG00000018914. Mus musculus.

Family and domain databases

Gene3D

1.20.1250.10. 1 hit.

InterPro

IPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR002183. Interleukin_3.
[Graphical view]

Pfam

PF02059. IL3. 1 hit.
[Graphical view]

PIRSF

PIRSF001939. IL-3. 1 hit.

PRINTS

PR00430. INTERLEUKIN3.

SUPFAM

SSF47266. 4_helix_cytokine. 1 hit.

ProtoNet

Search...

Other

NextBio

289085.

SOURCE

Search...

Entry information

Entry name

IL3_MOUSE

Accession

Primary (citable) accession number: P01586

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	April 3, 2013
This is version 116 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents