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P01586

- IL3_MOUSE

UniProt

P01586 - IL3_MOUSE

Protein

Interleukin-3

Gene

Il3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.
    This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.

    GO - Molecular functioni

    1. cytokine activity Source: MGI
    2. growth factor activity Source: MGI

    GO - Biological processi

    1. cytokine-mediated signaling pathway Source: MGI
    2. extrinsic apoptotic signaling pathway in absence of ligand Source: MGI
    3. immune response Source: InterPro
    4. inflammatory response Source: MGI
    5. myeloid cell apoptotic process Source: MGI
    6. negative regulation of apoptotic process Source: MGI
    7. negative regulation of autophagy Source: MGI
    8. negative regulation of mast cell apoptotic process Source: MGI
    9. positive regulation of cell proliferation Source: MGI
    10. positive regulation of JNK cascade Source: MGI
    11. positive regulation of mast cell proliferation Source: MGI
    12. positive regulation of myeloid leukocyte differentiation Source: MGI
    13. positive regulation of peptidyl-tyrosine phosphorylation Source: MGI
    14. positive regulation of protein phosphorylation Source: MGI
    15. regulation of gene expression Source: MGI
    16. regulation of glycolytic process Source: MGI
    17. regulation of protein dephosphorylation Source: MGI
    18. response to hypoxia Source: MGI

    Keywords - Molecular functioni

    Cytokine, Growth factor

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Interleukin-3
    Short name:
    IL-3
    Alternative name(s):
    Hematopoietic growth factor
    Mast cell growth factor
    Short name:
    MCGF
    Multipotential colony-stimulating factor
    P-cell-stimulating factor
    Gene namesi
    Name:Il3
    Synonyms:Csfmu, Il-3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:96552. Il3.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: MGI

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2626Add
    BLAST
    Chaini27 – 166140Interleukin-3PRO_0000015519Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi42 – 421N-linked (GlcNAc...)1 Publication
    Disulfide bondi43 ↔ 1061 Publication
    Disulfide bondi105 ↔ 1661 Publication
    Glycosylationi112 – 1121N-linked (GlcNAc...); partial1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiP01586.

    PTM databases

    PhosphoSiteiP01586.

    Expressioni

    Tissue specificityi

    Activated T-cells, mast cells, natural killer cells.

    Gene expression databases

    ArrayExpressiP01586.
    BgeeiP01586.
    CleanExiMM_IL3.
    GenevestigatoriP01586.

    Interactioni

    Subunit structurei

    Monomer.

    Structurei

    Secondary structure

    1
    166
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi37 – 415
    Helixi44 – 5310
    Helixi67 – 693
    Turni71 – 733
    Helixi74 – 8512
    Turni86 – 883
    Helixi90 – 934
    Helixi97 – 10610
    Helixi124 – 13714
    Beta strandi140 – 1423
    Beta strandi151 – 1533

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2L3ONMR-A33-156[»]
    ProteinModelPortaliP01586.
    SMRiP01586. Positions 33-156.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the IL-3 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiopiNOG27251.
    HOGENOMiHOG000113051.
    HOVERGENiHBG006148.
    InParanoidiP01586.
    KOiK04736.
    OMAiNDSALPG.
    OrthoDBiEOG7BZVW6.
    PhylomeDBiP01586.
    TreeFamiTF338567.

    Family and domain databases

    Gene3Di1.20.1250.10. 1 hit.
    InterProiIPR009079. 4_helix_cytokine-like_core.
    IPR012351. 4_helix_cytokine_core.
    IPR002183. IL-3.
    [Graphical view]
    PANTHERiPTHR10079. PTHR10079. 1 hit.
    PfamiPF02059. IL3. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001939. IL-3. 1 hit.
    PRINTSiPR00430. INTERLEUKIN3.
    SUPFAMiSSF47266. SSF47266. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P01586-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVLASSTTSI HTMLLLLLML FHLGLQASIS GRDTHRLTRT LNCSSIVKEI    50
    IGKLPEPELK TDDEGPSLRN KSFRRVNLSK FVESQGEVDP EDRYVIKSNL 100
    QKLNCCLPTS ANDSALPGVF IRDLDDFRKK LRFYMVHLND LETVLTSRPP 150
    QPASGSVSPN RGTVEC 166
    Length:166
    Mass (Da):18,540
    Last modified:July 21, 1986 - v1
    Checksum:iF38EE7A9E361CAA2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K01850 mRNA. Translation: AAA39291.1.
    K01668 mRNA. Translation: AAA39507.1.
    K03233 Genomic DNA. Translation: AAA39293.1.
    X02732 Genomic DNA. Translation: CAA26514.1.
    M20128, M14394 Genomic DNA. Translation: AAA39308.1.
    CCDSiCCDS24693.1.
    PIRiA25481. ICMS3.
    RefSeqiNP_034686.2. NM_010556.4.
    UniGeneiMm.983.

    Genome annotation databases

    EnsembliENSMUST00000019058; ENSMUSP00000019058; ENSMUSG00000018914.
    GeneIDi16187.
    KEGGimmu:16187.
    UCSCiuc007ixn.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K01850 mRNA. Translation: AAA39291.1 .
    K01668 mRNA. Translation: AAA39507.1 .
    K03233 Genomic DNA. Translation: AAA39293.1 .
    X02732 Genomic DNA. Translation: CAA26514.1 .
    M20128 , M14394 Genomic DNA. Translation: AAA39308.1 .
    CCDSi CCDS24693.1.
    PIRi A25481. ICMS3.
    RefSeqi NP_034686.2. NM_010556.4.
    UniGenei Mm.983.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2L3O NMR - A 33-156 [» ]
    ProteinModelPortali P01586.
    SMRi P01586. Positions 33-156.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei P01586.

    Proteomic databases

    PRIDEi P01586.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000019058 ; ENSMUSP00000019058 ; ENSMUSG00000018914 .
    GeneIDi 16187.
    KEGGi mmu:16187.
    UCSCi uc007ixn.1. mouse.

    Organism-specific databases

    CTDi 3562.
    MGIi MGI:96552. Il3.

    Phylogenomic databases

    eggNOGi opiNOG27251.
    HOGENOMi HOG000113051.
    HOVERGENi HBG006148.
    InParanoidi P01586.
    KOi K04736.
    OMAi NDSALPG.
    OrthoDBi EOG7BZVW6.
    PhylomeDBi P01586.
    TreeFami TF338567.

    Miscellaneous databases

    NextBioi 289085.
    PROi P01586.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P01586.
    Bgeei P01586.
    CleanExi MM_IL3.
    Genevestigatori P01586.

    Family and domain databases

    Gene3Di 1.20.1250.10. 1 hit.
    InterProi IPR009079. 4_helix_cytokine-like_core.
    IPR012351. 4_helix_cytokine_core.
    IPR002183. IL-3.
    [Graphical view ]
    PANTHERi PTHR10079. PTHR10079. 1 hit.
    Pfami PF02059. IL3. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001939. IL-3. 1 hit.
    PRINTSi PR00430. INTERLEUKIN3.
    SUPFAMi SSF47266. SSF47266. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Isolation and characterization of a mouse cDNA clone that expresses mast-cell growth-factor activity in monkey cells."
      Yokota T., Lee F., Rennick D., Hall C., Arai N., Mosmann T., Nabel G., Cantor H., Arai K.
      Proc. Natl. Acad. Sci. U.S.A. 81:1070-1074(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Structure of the chromosomal gene for murine interleukin 3."
      Miyatake S., Yokota T., Lee F., Arai K.
      Proc. Natl. Acad. Sci. U.S.A. 82:316-320(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "Cloning and nucleotide sequence of the murine interleukin-3 gene."
      Campbell H.D., Ymer S., Fung M.-C., Young I.G.
      Eur. J. Biochem. 150:297-304(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    5. "Isolation and characterization of a genomic DDD mouse interleukin-3 gene."
      Todokoro K., Yamamoto A., Amanuma H., Ikawa Y.
      Gene 39:103-107(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: DDD.
    6. "Determination of the glycosylation patterns, disulfide linkages, and protein heterogeneities of baculovirus-expressed mouse interleukin-3 by mass spectrometry."
      Knepper T.P., Arbogast B., Schreurs J., Deinzer M.L.
      Biochemistry 31:11651-11659(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION AT ASN-42 AND ASN-112, DISULFIDE BONDS.
    7. "Murine interleukin-3: structure, dynamics, and conformational heterogeneity in solution."
      Yao S., Young I.G., Norton R.S., Murphy J.M.
      Biochemistry 50:2464-2477(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 33-156, DISULFIDE BOND.

    Entry informationi

    Entry nameiIL3_MOUSE
    AccessioniPrimary (citable) accession number: P01586
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 127 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3