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Protein

Interleukin-3

Gene

Il3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.
This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.

GO - Molecular functioni

  • cytokine activity Source: MGI
  • growth factor activity Source: MGI
  • interleukin-3 receptor binding Source: GO_Central

GO - Biological processi

  • cytokine-mediated signaling pathway Source: MGI
  • extrinsic apoptotic signaling pathway in absence of ligand Source: MGI
  • immune response Source: InterPro
  • myeloid cell apoptotic process Source: MGI
  • negative regulation of apoptotic process Source: MGI
  • negative regulation of autophagy Source: MGI
  • negative regulation of mast cell apoptotic process Source: MGI
  • positive regulation of cell proliferation Source: MGI
  • positive regulation of JNK cascade Source: MGI
  • positive regulation of mast cell proliferation Source: MGI
  • positive regulation of myeloid leukocyte differentiation Source: MGI
  • positive regulation of peptidyl-tyrosine phosphorylation Source: MGI
  • positive regulation of protein phosphorylation Source: MGI
  • regulation of gene expression Source: MGI
  • regulation of glycolytic process Source: MGI
  • regulation of protein dephosphorylation Source: MGI
  • response to hypoxia Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Growth factor

Names & Taxonomyi

Protein namesi
Recommended name:
Interleukin-3
Short name:
IL-3
Alternative name(s):
Hematopoietic growth factor
Mast cell growth factor
Short name:
MCGF
Multipotential colony-stimulating factor
P-cell-stimulating factor
Gene namesi
Name:Il3
Synonyms:Csfmu, Il-3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:96552. Il3.

Subcellular locationi

GO - Cellular componenti

  • extracellular space Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Add
BLAST
Chaini27 – 166140Interleukin-3PRO_0000015519Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi42 – 421N-linked (GlcNAc...)1 Publication
Disulfide bondi43 ↔ 1061 Publication
Disulfide bondi105 ↔ 1661 Publication
Glycosylationi112 – 1121N-linked (GlcNAc...); partial1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP01586.

PTM databases

PhosphoSiteiP01586.

Expressioni

Tissue specificityi

Activated T-cells, mast cells, natural killer cells.

Gene expression databases

BgeeiP01586.
CleanExiMM_IL3.
ExpressionAtlasiP01586. baseline and differential.
GenevisibleiP01586. MM.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000019058.

Structurei

Secondary structure

1
166
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi37 – 415Combined sources
Helixi44 – 5310Combined sources
Helixi67 – 693Combined sources
Turni71 – 733Combined sources
Helixi74 – 8512Combined sources
Turni86 – 883Combined sources
Helixi90 – 934Combined sources
Helixi97 – 10610Combined sources
Helixi124 – 13714Combined sources
Beta strandi140 – 1423Combined sources
Beta strandi151 – 1533Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2L3ONMR-A33-156[»]
ProteinModelPortaliP01586.
SMRiP01586. Positions 33-156.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the IL-3 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiopiNOG27251.
HOGENOMiHOG000113051.
HOVERGENiHBG006148.
InParanoidiP01586.
KOiK04736.
OMAiSGRDTHR.
OrthoDBiEOG7BZVW6.
PhylomeDBiP01586.
TreeFamiTF338567.

Family and domain databases

Gene3Di1.20.1250.10. 1 hit.
InterProiIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR002183. IL-3.
[Graphical view]
PANTHERiPTHR10079. PTHR10079. 1 hit.
PfamiPF02059. IL3. 1 hit.
[Graphical view]
PIRSFiPIRSF001939. IL-3. 1 hit.
PRINTSiPR00430. INTERLEUKIN3.
SUPFAMiSSF47266. SSF47266. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01586-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLASSTTSI HTMLLLLLML FHLGLQASIS GRDTHRLTRT LNCSSIVKEI
60 70 80 90 100
IGKLPEPELK TDDEGPSLRN KSFRRVNLSK FVESQGEVDP EDRYVIKSNL
110 120 130 140 150
QKLNCCLPTS ANDSALPGVF IRDLDDFRKK LRFYMVHLND LETVLTSRPP
160
QPASGSVSPN RGTVEC
Length:166
Mass (Da):18,540
Last modified:July 21, 1986 - v1
Checksum:iF38EE7A9E361CAA2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01850 mRNA. Translation: AAA39291.1.
K01668 mRNA. Translation: AAA39507.1.
K03233 Genomic DNA. Translation: AAA39293.1.
X02732 Genomic DNA. Translation: CAA26514.1.
M20128, M14394 Genomic DNA. Translation: AAA39308.1.
CCDSiCCDS24693.1.
PIRiA25481. ICMS3.
RefSeqiNP_034686.2. NM_010556.4.
UniGeneiMm.983.

Genome annotation databases

EnsembliENSMUST00000019058; ENSMUSP00000019058; ENSMUSG00000018914.
GeneIDi16187.
KEGGimmu:16187.
UCSCiuc007ixn.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01850 mRNA. Translation: AAA39291.1.
K01668 mRNA. Translation: AAA39507.1.
K03233 Genomic DNA. Translation: AAA39293.1.
X02732 Genomic DNA. Translation: CAA26514.1.
M20128, M14394 Genomic DNA. Translation: AAA39308.1.
CCDSiCCDS24693.1.
PIRiA25481. ICMS3.
RefSeqiNP_034686.2. NM_010556.4.
UniGeneiMm.983.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2L3ONMR-A33-156[»]
ProteinModelPortaliP01586.
SMRiP01586. Positions 33-156.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000019058.

PTM databases

PhosphoSiteiP01586.

Proteomic databases

PRIDEiP01586.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000019058; ENSMUSP00000019058; ENSMUSG00000018914.
GeneIDi16187.
KEGGimmu:16187.
UCSCiuc007ixn.1. mouse.

Organism-specific databases

CTDi3562.
MGIiMGI:96552. Il3.

Phylogenomic databases

eggNOGiopiNOG27251.
HOGENOMiHOG000113051.
HOVERGENiHBG006148.
InParanoidiP01586.
KOiK04736.
OMAiSGRDTHR.
OrthoDBiEOG7BZVW6.
PhylomeDBiP01586.
TreeFamiTF338567.

Miscellaneous databases

NextBioi289085.
PROiP01586.
SOURCEiSearch...

Gene expression databases

BgeeiP01586.
CleanExiMM_IL3.
ExpressionAtlasiP01586. baseline and differential.
GenevisibleiP01586. MM.

Family and domain databases

Gene3Di1.20.1250.10. 1 hit.
InterProiIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR002183. IL-3.
[Graphical view]
PANTHERiPTHR10079. PTHR10079. 1 hit.
PfamiPF02059. IL3. 1 hit.
[Graphical view]
PIRSFiPIRSF001939. IL-3. 1 hit.
PRINTSiPR00430. INTERLEUKIN3.
SUPFAMiSSF47266. SSF47266. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Isolation and characterization of a mouse cDNA clone that expresses mast-cell growth-factor activity in monkey cells."
    Yokota T., Lee F., Rennick D., Hall C., Arai N., Mosmann T., Nabel G., Cantor H., Arai K.
    Proc. Natl. Acad. Sci. U.S.A. 81:1070-1074(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Structure of the chromosomal gene for murine interleukin 3."
    Miyatake S., Yokota T., Lee F., Arai K.
    Proc. Natl. Acad. Sci. U.S.A. 82:316-320(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Cloning and nucleotide sequence of the murine interleukin-3 gene."
    Campbell H.D., Ymer S., Fung M.-C., Young I.G.
    Eur. J. Biochem. 150:297-304(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. "Isolation and characterization of a genomic DDD mouse interleukin-3 gene."
    Todokoro K., Yamamoto A., Amanuma H., Ikawa Y.
    Gene 39:103-107(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DDD.
  6. "Determination of the glycosylation patterns, disulfide linkages, and protein heterogeneities of baculovirus-expressed mouse interleukin-3 by mass spectrometry."
    Knepper T.P., Arbogast B., Schreurs J., Deinzer M.L.
    Biochemistry 31:11651-11659(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION AT ASN-42 AND ASN-112, DISULFIDE BONDS.
  7. "Murine interleukin-3: structure, dynamics, and conformational heterogeneity in solution."
    Yao S., Young I.G., Norton R.S., Murphy J.M.
    Biochemistry 50:2464-2477(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 33-156, DISULFIDE BOND.

Entry informationi

Entry nameiIL3_MOUSE
AccessioniPrimary (citable) accession number: P01586
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: June 24, 2015
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.