P01581 (IFNG_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Interferon gamma
Short name=IFN-gamma

Gene names

Name:

Ifng

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	156 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
Subunit structure	Homodimer.
Subcellular location	Secreted.
Tissue specificity	Released primarily from activated T lymphocytes.
Sequence similarities	Belongs to the type II (or gamma) interferon family.

Ontologies

Keywords
Biological process	Antiviral defense Growth regulation
Cellular component	Secreted
Domain	Signal
Molecular function	Cytokine
PTM	Glycoprotein Pyrrolidone carboxylic acid
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	CD8-positive, alpha-beta T cell differentiation involved in immune response Inferred from electronic annotation. Source: Compara adaptive immune response Inferred from electronic annotation. Source: Compara antigen processing and presentation Inferred from electronic annotation. Source: Compara cell cycle arrest Inferred from electronic annotation. Source: Compara cellular response to interleukin-18 Inferred from electronic annotation. Source: Compara cellular response to lipopolysaccharide Inferred from electronic annotation. Source: Compara defense response to bacterium Inferred from electronic annotation. Source: Compara defense response to protozoan Inferred from electronic annotation. Source: Compara defense response to virus Inferred from electronic annotation. Source: UniProtKB-KW endoplasmic reticulum unfolded protein response Inferred from electronic annotation. Source: Compara humoral immune response Inferred from electronic annotation. Source: Compara negative regulation of cell proliferation Inferred from direct assay PubMed 11940252. Source: RGD negative regulation of growth of symbiont in host Inferred from electronic annotation. Source: Compara negative regulation of interleukin-17 production Inferred from electronic annotation. Source: Compara negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis Inferred from direct assay PubMed 20624457. Source: BHF-UCL negative regulation of metanephric nephron tubule epithelial cell differentiation Inferred from direct assay PubMed 20624457. Source: BHF-UCL negative regulation of myelination Inferred from electronic annotation. Source: Compara negative regulation of smooth muscle cell proliferation Inferred from electronic annotation. Source: Compara negative regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 20624457. Source: BHF-UCL neutrophil apoptotic process Inferred from electronic annotation. Source: Compara neutrophil chemotaxis Inferred from electronic annotation. Source: Compara positive regulation of MHC class II biosynthetic process Inferred from electronic annotation. Source: Compara positive regulation of T cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of calcidiol 1-monooxygenase activity Inferred from electronic annotation. Source: Compara positive regulation of cell adhesion Inferred from direct assay PubMed 2509328. Source: RGD positive regulation of chemokine biosynthetic process Inferred from electronic annotation. Source: Compara positive regulation of fructose 1,6-bisphosphate 1-phosphatase activity Inferred from electronic annotation. Source: Compara positive regulation of fructose 1,6-bisphosphate metabolic process Inferred from electronic annotation. Source: Compara positive regulation of interleukin-1 beta secretion Inferred from electronic annotation. Source: Compara positive regulation of interleukin-12 biosynthetic process Inferred from electronic annotation. Source: Compara positive regulation of interleukin-12 production Inferred from electronic annotation. Source: Compara positive regulation of interleukin-23 production Inferred from electronic annotation. Source: Compara positive regulation of interleukin-6 biosynthetic process Inferred from electronic annotation. Source: Compara positive regulation of isotype switching to IgG isotypes Inferred from electronic annotation. Source: Compara positive regulation of killing of cells of other organism Inferred from electronic annotation. Source: Compara positive regulation of membrane protein ectodomain proteolysis Inferred from electronic annotation. Source: Compara positive regulation of mesenchymal cell proliferation Inferred from direct assay PubMed 20624457. Source: BHF-UCL positive regulation of neuron differentiation Inferred from direct assay PubMed 19355840. Source: RGD positive regulation of nitric oxide biosynthetic process Inferred from electronic annotation. Source: Compara positive regulation of osteoclast differentiation Inferred from electronic annotation. Source: Compara positive regulation of peptidyl-serine phosphorylation of STAT protein Inferred from direct assay PubMed 20624457. Source: BHF-UCL positive regulation of smooth muscle cell apoptotic process Inferred from electronic annotation. Source: Compara positive regulation of synaptic transmission, cholinergic Inferred from direct assay PubMed 1900518. Source: RGD positive regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara positive regulation of tumor necrosis factor (ligand) superfamily member 11 production Inferred from electronic annotation. Source: Compara positive regulation of tumor necrosis factor production Inferred from electronic annotation. Source: Compara positive regulation of tyrosine phosphorylation of Stat1 protein Inferred from direct assay PubMed 20624457. Source: BHF-UCL positive regulation of vitamin D biosynthetic process Inferred from electronic annotation. Source: Compara protein import into nucleus, translocation Inferred from electronic annotation. Source: Compara regulation of insulin secretion Inferred from electronic annotation. Source: Compara regulation of the force of heart contraction Inferred from direct assay PubMed 1900518. Source: RGD response to drug Inferred from expression pattern PubMed 19439114. Source: RGD
Cellular_component	external side of plasma membrane Inferred from electronic annotation. Source: Compara extracellular space Inferred from direct assay PubMed 2474612Ref.1. Source: RGD
Molecular_function	cytokine activity Traceable author statement PubMed 12162878. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	By similarity
	Chain	23 – 156	134	Interferon gamma		PRO_0000016457
Amino acid modifications
	Modified residue	23	1	Pyrrolidone carboxylic acid By similarity
	Glycosylation	38	1	N-linked (GlcNAc...) By similarity
	Glycosylation	91	1	N-linked (GlcNAc...) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P01581 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: BC0B433D2CE1E3F2	FASTA	156	17,918
	10 20 30 40 50 60 MSATRRVLVL QLCLMALSGC YCQGTLIESL ESLKNYFNSS SMDAMEGKSL LLDIWRNWQK 70 80 90 100 110 120 DGNTKILESQ IISFYLRLFE VLKDNQAISN NISVIESHLI TNFFSNSKAK KDAFMSIAKF 130 140 150 EVNNPQIQHK AVNELIRVIH QLSPESSLRK RKRSRC

References

[1]	"Cloning and expression of the chromosomal immune interferon gene of the rat." Dijkema R., van der Meide P.H., Pouwels P.H., Caspers M., Dubbeld M., Schellekens H. EMBO J. 4:761-767(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Cloning, expression, and purification of rat IFN-gamma." Dijkema R., der Meide P.H., Dubbeld M., Caspers M., Wubben J., Schellekens H. Methods Enzymol. 119:453-463(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	Widegren B., Visse E., Sjogren H.O. Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Fischer.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X02325 Genomic DNA. Translation: CAA26185.1. X02326 Genomic DNA. Translation: CAA26186.1. X02326 Genomic DNA. Translation: CAA26187.1. X02327 Genomic DNA. Translation: CAA26188.1. M29317, M29315, M29316 Genomic DNA. Translation: AAA41362.1. AF010466 mRNA. Translation: AAB66352.1.
IPI	IPI00214504.
PIR	IVRTG. A01845.
RefSeq	NP_620235.1. NM_138880.2.
UniGene	Rn.10795.
3D structure databases
ProteinModelPortal	P01581.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000009917.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000009919; ENSRNOP00000009917; ENSRNOG00000007468.
GeneID	25712.
KEGG	rno:25712.
UCSC	RGD:2866. rat.
Organism-specific databases
CTD	3458.
RGD	2866. Ifng.
Phylogenomic databases
eggNOG	NOG45353.
GeneTree	ENSGT00390000007831.
HOGENOM	HOG000254784.
HOVERGEN	HBG056912.
InParanoid	P01581.
KO	K04687.
OMA	KEYFNAS.
OrthoDB	EOG4MCX1G.
Gene expression databases
Genevestigator	P01581.
GermOnline	ENSRNOG00000007468. Rattus norvegicus.
Family and domain databases
Gene3D	1.20.1250.10. 1 hit.
InterPro	IPR009079. 4_helix_cytokine-like_core. IPR012351. 4_helix_cytokine_core. IPR002069. Interferon_gamma. [Graphical view]
PANTHER	PTHR11419. PTHR11419. 1 hit.
Pfam	PF00714. IFN-gamma. 1 hit. [Graphical view]
PIRSF	PIRSF001936. IFN-gamma. 1 hit.
ProDom	PD002435. Interferon_gamma. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF47266. 4_helix_cytokine. 1 hit.
ProtoNet	Search...
Other
NextBio	607779.

Entry information

Entry name

IFNG_RAT

Accession

Primary (citable) accession number: P01581

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	April 3, 2013
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program