P01580 (IFNG_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Interferon gamma
Short name=IFN-gamma

Gene names

Name:

Ifng

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	155 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
Subunit structure	Homodimer.
Subcellular location	Secreted.
Tissue specificity	Released primarily from activated T lymphocytes.
Sequence similarities	Belongs to the type II (or gamma) interferon family.

Ontologies

Keywords
Biological process	Antiviral defense Growth regulation
Cellular component	Secreted
Domain	Signal
Molecular function	Cytokine
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	CD8-positive, alpha-beta T cell differentiation involved in immune response Inferred from direct assay PubMed 17277142. Source: MGI adaptive immune response Inferred from direct assay PubMed 17277142. Source: MGI antigen processing and presentation Inferred from direct assay PubMed 15356141. Source: MGI cell cycle arrest Inferred from electronic annotation. Source: Compara cellular response to interleukin-18 Inferred from direct assay PubMed 19666510. Source: MGI cellular response to lipopolysaccharide Inferred from direct assay PubMed 18791161. Source: MGI defense response to bacterium Inferred from direct assay PubMed 15240719. Source: MGI defense response to protozoan Inferred from mutant phenotype PubMed 9348310. Source: MGI defense response to virus Inferred from direct assay PubMed 20064371. Source: MGI endoplasmic reticulum unfolded protein response Inferred from direct assay PubMed 15911877. Source: MGI humoral immune response Inferred from direct assay PubMed 17277142. Source: MGI negative regulation of growth of symbiont in host Inferred from mutant phenotype PubMed 9348310. Source: MGI negative regulation of interleukin-17 production Inferred from electronic annotation. Source: Compara negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis Inferred from electronic annotation. Source: Compara negative regulation of metanephric nephron tubule epithelial cell differentiation Inferred from electronic annotation. Source: Compara negative regulation of myelination Inferred from direct assay PubMed 15911877. Source: MGI negative regulation of smooth muscle cell proliferation Inferred from electronic annotation. Source: Compara negative regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara neutrophil apoptotic process Inferred from mutant phenotype PubMed 12925700. Source: MGI neutrophil chemotaxis Inferred from direct assay PubMed 12925700. Source: MGI positive regulation of MHC class II biosynthetic process Inferred from direct assay PubMed 1845803PubMed 2493384PubMed 2502420. Source: BHF-UCL positive regulation of T cell proliferation Inferred from direct assay PubMed 16280321. Source: MGI positive regulation of calcidiol 1-monooxygenase activity Inferred from electronic annotation. Source: Compara positive regulation of cell adhesion Inferred from electronic annotation. Source: Compara positive regulation of chemokine biosynthetic process Inferred from direct assay PubMed 12925700. Source: MGI positive regulation of fructose 1,6-bisphosphate 1-phosphatase activity Inferred from electronic annotation. Source: Compara positive regulation of fructose 1,6-bisphosphate metabolic process Inferred from electronic annotation. Source: Compara positive regulation of interleukin-1 beta secretion Inferred from mutant phenotype PubMed 12925700. Source: MGI positive regulation of interleukin-12 biosynthetic process Inferred from direct assay PubMed 14568984. Source: MGI positive regulation of interleukin-12 production Inferred from direct assay PubMed 9348310. Source: MGI positive regulation of interleukin-23 production Inferred from electronic annotation. Source: Compara positive regulation of interleukin-6 biosynthetic process Inferred from direct assay PubMed 12925700. Source: MGI positive regulation of isotype switching to IgG isotypes Inferred from direct assay PubMed 12882831. Source: MGI positive regulation of killing of cells of other organism Inferred from electronic annotation. Source: Compara positive regulation of membrane protein ectodomain proteolysis Inferred from electronic annotation. Source: Compara positive regulation of mesenchymal cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of neuron differentiation Inferred from electronic annotation. Source: Compara positive regulation of nitric oxide biosynthetic process Inferred from direct assay PubMed 11168643. Source: MGI positive regulation of osteoclast differentiation Inferred from electronic annotation. Source: Compara positive regulation of peptidyl-serine phosphorylation of STAT protein Inferred from sequence orthology PubMed 16280321. Source: MGI positive regulation of smooth muscle cell apoptotic process Inferred from electronic annotation. Source: Compara positive regulation of synaptic transmission, cholinergic Inferred from electronic annotation. Source: Compara positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 18025219. Source: MGI positive regulation of tumor necrosis factor (ligand) superfamily member 11 production Inferred from electronic annotation. Source: Compara positive regulation of tumor necrosis factor production Inferred from direct assay PubMed 1845803PubMed 2502420. Source: BHF-UCL positive regulation of tyrosine phosphorylation of Stat1 protein Inferred from sequence orthology PubMed 16280321. Source: MGI positive regulation of vitamin D biosynthetic process Inferred from electronic annotation. Source: Compara protein import into nucleus, translocation Inferred from electronic annotation. Source: Compara regulation of insulin secretion Inferred from electronic annotation. Source: Compara regulation of the force of heart contraction Inferred from electronic annotation. Source: Compara response to drug Inferred from electronic annotation. Source: Compara
Cellular_component	external side of plasma membrane Inferred from direct assay PubMed 16177108PubMed 17658279PubMed 19838199PubMed 20399120PubMed 20956546PubMed 21217760PubMed 21606376PubMed 21613615PubMed 21709260. Source: MGI extracellular space Inferred from direct assay PubMed 10072077PubMed 11057672PubMed 11728336PubMed 11894098PubMed 15220916PubMed 15240714PubMed 16275384PubMed 16769892PubMed 17082577PubMed 17277142PubMed 18997793PubMed 19139201PubMed 20706986PubMed 21460847PubMed 9348310. Source: MGI
Molecular_function	cytokine activity Inferred from direct assay PubMed 11027433PubMed 11911824PubMed 12925700PubMed 15240719. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 155	133	Interferon gamma		PRO_0000016453
Amino acid modifications
	Glycosylation	38	1	N-linked (GlcNAc...) By similarity
	Glycosylation	90	1	N-linked (GlcNAc...) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P01580 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 7EE80BAECB42071A	FASTA	155	17,907
	10 20 30 40 50 60 MNATHCILAL QLFLMAVSGC YCHGTVIESL ESLNNYFNSS GIDVEEKSLF LDIWRNWQKD 70 80 90 100 110 120 GDMKILQSQI ISFYLRLFEV LKDNQAISNN ISVIESHLIT TFFSNSKAKK DAFMSIAKFE 130 140 150 VNNPQVQRQA FNELIRVVHQ LLPESSLRKR KRSRC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of murine immune interferon cDNA." Gray P.W., Goeddel D.V. Proc. Natl. Acad. Sci. U.S.A. 80:5842-5846(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: NOD. Tissue: Spleen.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	K00083 mRNA. Translation: AAA37894.1. AK089574 mRNA. Translation: BAC40926.1. BC119063 mRNA. Translation: AAI19064.1. BC119065 mRNA. Translation: AAI19066.1.
IPI	IPI00136892.
PIR	IVMSG. A01844.
RefSeq	NP_032363.1. NM_008337.3.
UniGene	Mm.240327.
3D structure databases
ProteinModelPortal	P01580.
SMR	P01580. Positions 30-141.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000063800.
PTM databases
PhosphoSite	P01580.
Proteomic databases
PRIDE	P01580.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000068592; ENSMUSP00000063800; ENSMUSG00000055170.
GeneID	15978.
KEGG	mmu:15978.
UCSC	uc007hdy.1. mouse.
Organism-specific databases
CTD	3458.
MGI	MGI:107656. Ifng.
Phylogenomic databases
eggNOG	NOG45353.
GeneTree	ENSGT00390000007831.
HOGENOM	HOG000254784.
HOVERGEN	HBG056912.
InParanoid	P01580.
KO	K04687.
OMA	KEYFNAS.
OrthoDB	EOG4MCX1G.
Gene expression databases
ArrayExpress	P01580.
Bgee	P01580.
CleanEx	MM_IFNG.
Genevestigator	P01580.
GermOnline	ENSMUSG00000055170. Mus musculus.
Family and domain databases
Gene3D	1.20.1250.10. 1 hit.
InterPro	IPR009079. 4_helix_cytokine-like_core. IPR012351. 4_helix_cytokine_core. IPR002069. Interferon_gamma. [Graphical view]
PANTHER	PTHR11419. PTHR11419. 1 hit.
Pfam	PF00714. IFN-gamma. 1 hit. [Graphical view]
PIRSF	PIRSF001936. IFN-gamma. 1 hit.
ProDom	PD002435. Interferon_gamma. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF47266. 4_helix_cytokine. 1 hit.
ProtoNet	Search...
Other
NextBio	288760.
SOURCE	Search...

Entry information

Entry name

IFNG_MOUSE

Accession

Primary (citable) accession number: P01580
Secondary accession number(s): Q542B8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	May 1, 2013
This is version 112 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program