Reviewed, UniProtKB/Swiss-Prot P01574 (IFNB_HUMAN)

Names and origin

Protein names

Recommended name:
    Interferon beta
      Short name(s)=IFN-beta
Alternative name(s):
    Fibroblast interferon

Gene names

Name:	IFNB1
Synonyms:	IFB, IFNB

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	187 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Has antiviral, antibacterial and anticancer activities.
Subunit structure	Monomer.
Subcellular location	Secreted.
Pharmaceutical use	Available under the names Avonex (Biogen), Betaseron (Berlex) and Rebif (Serono). Used in the treatment of multiple sclerosis (MS). Betaseron is a slightly modified form of IFNB1 with two residue substitutions.
Sequence similarities	Belongs to the alpha/beta interferon family.

Ontologies

Keywords
Biological process	Antiviral defense
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Cytokine
PTM	Glycoprotein Phosphoprotein
Technical term	3D-structure Pharmaceutical
Gene Ontology (GO)
Biological process	B cell proliferation Non-traceable author statement. Source: UniProtKB caspase activation Inferred from direct assay. Source: UniProtKB cell surface receptor linked signal transduction Traceable author statement. Source: ProtInc induction of apoptosis Inferred from direct assay. Source: UniProtKB natural killer cell activation Non-traceable author statement. Source: UniProtKB negative regulation of cell proliferation Non-traceable author statement. Source: UniProtKB negative regulation of virion penetration into host cell Non-traceable author statement. Source: UniProtKB positive regulation of innate immune response Non-traceable author statement. Source: UniProtKB regulation of MHC class I biosynthetic process Non-traceable author statement. Source: UniProtKB response to virus Non-traceable author statement. Source: UniProtKB
Cellular component	extracellular region Ref.7 Inferred by curator. Source: UniProtKB
Molecular function	interferon-alpha/beta receptor binding Ref.7 Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Molecule processing

Signal peptide

1 – 21

21

Chain

22 – 187

166

Interferon beta

Amino acid modifications

Modified residue

140

1

Phosphoserine

Glycosylation

101

1

N-linked (GlcNAc...)

Disulfide bond

52 ↔ 162

Natural variations

Natural variant

162

1

C → Y in clone PF526, loss of ability to form the essential disulfide bond, loss of antiviral activity.

Natural variant

164

1

W → C in a breast cancer sample; somatic mutation.

Secondary structure

1

.

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.

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.

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187

Helix Strand Turn

Details...

Helix

24 – 42

19

Helix

51 – 55

5

Helix

63 – 67

5

Helix

73 – 91

19

Helix

96 – 98

3

Helix

102 – 127

26

Turn

137 – 139

3

Helix

140 – 156

17

Turn

157 – 159

3

Helix

161 – 182

22

Sequences

	Sequence		Length	Mass (Da)
	P01574-1 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 0B013D4087723CEC	FASTA	187	22,294
	10 20 30 40 50 60 MTNKCLLQIA LLLCFSTTAL SMSYNLLGFL QRSSNFQCQK LLWQLNGRLE YCLKDRMNFD 70 80 90 100 110 120 IPEEIKQLQQ FQKEDAALTI YEMLQNIFAI FRQDSSSTGW NETIVENLLA NVYHQINHLK 130 140 150 160 170 180 TVLEEKLEKE DFTRGKLMSS LHLKRYYGRI LHYLKAKEYS HCAWTIVRVE ILRNFYFINR LTGYLRN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human fibroblast interferon gene lacks introns." Lawn R.M., Adelman J., Franke A.E., Houck C.M., Gross M., Najarian R., Goeddel D.V. Nucleic Acids Res. 9:1045-1052(1981) [PubMed: 6164984] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Structure of a chromosomal gene for human interferon beta." Ohno S., Taniguchi T. Proc. Natl. Acad. Sci. U.S.A. 78:5305-5309(1981) [PubMed: 16593086] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[3]	"The nucleotide sequence of human fibroblast interferon cDNA." Taniguchi T., Ohno S., Fujii-Kuriyama Y., Muramatsu M. Gene 10:11-15(1980) [PubMed: 6157601] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[4]	"Isolation and structure of a human fibroblast interferon gene." Derynck R., Content J., Declercq E., Volckaert G., Tavernier J., Devos R., Fiers W. Nature 285:542-547(1980) [PubMed: 6157094] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[5]	"The complete amino acid sequence of human fibroblast interferon as deduced using synthetic oligodeoxyribonucleotide primers of reverse transcriptase." Houghton M., Eaton M.A.W., Stewart A.G., Smith J.C., Doel S.M., Cartlin G.H., Lewis H.M., Patel T.P., Emtage J.S., Carey N.H., Porter A.G. Nucleic Acids Res. 8:2885-2894(1980) [PubMed: 6159580] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[6]	"Synthesis of human fibroblast interferon by E. coli." Goeddel D.V., Shepard H.M., Yelverton E., Leung D., Crea R., Sloma A., Pestka S. Nucleic Acids Res. 8:4057-4074(1980) [PubMed: 6159584] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[7]	"On the relationship between human interferon alpha 1 and beta 1 genes." May L.T., Sehgal P.B. J. Interferon Res. 5:521-526(1985) [PubMed: 2414376] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[9]	"The amino-terminal sequence of human fibroblast interferon as deduced from reverse transcripts obtained using synthetic oligonucleotide primers." Houghton M., Stewart A.G., Doel S.M., Emtage J.S., Eaton M.A.W., Smith J.C., Patel T.P., Lewis H.M., Porter A.G., Birch J.R., Cartwright T., Carey N.H. Nucleic Acids Res. 8:1913-1931(1980) [PubMed: 6159597] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 1-68.
[10]	"Assignment of the disulphide bonds of leukocyte interferon." Wetzel R. Nature 289:606-607(1981) [PubMed: 6162107] [Abstract] Cited for: DISULFIDE BOND.
[11]	"A single amino acid change in IFN-beta1 abolishes its antiviral activity." Shepard H.M., Leung D., Stebbing N., Goeddel D.V. Nature 294:563-565(1981) [PubMed: 6171735] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 71-187 (VARIANT CLONE PF526).
[12]	"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column." Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y. Anal. Sci. 24:161-166(2008) [PubMed: 18187866] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-140, MASS SPECTROMETRY.
[13]	"The crystal structure of human interferon beta at 2.2-A resolution." Karpusas M., Nolte M., Benton C.B., Meier W., Lipscomb W.N., Goelz S. Proc. Natl. Acad. Sci. U.S.A. 94:11813-11818(1997) [PubMed: 9342320] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
[14]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] CYS-164.

Web resources

Avonex

Clinical information on Avonex

Betaseron

Clinical information on Betaseron

Cross-references

Sequence databases

EMBLGenBankDDBJ

V00534 Genomic DNA. Translation: CAA23795.1.
V00535 Genomic DNA. Translation: CAA23796.1.
V00546 mRNA. Translation: CAA23807.1.
V00547 mRNA. Translation: CAA23808.1.
BC069314 mRNA. Translation: AAH69314.1.
M28622 mRNA. Translation: AAA36040.1.

PIR

IVHUB1. A93721.

RefSeq

NP_002167.1.

UniGene

Hs.93177

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1AU1	X-ray	2.20	A/B	22-187	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:6018N.

PTM databases

PhosphoSite

P01574.

Genome annotation databases

Ensembl

ENSG00000171855. Homo sapiens. [Contig view]

GeneID

3456.

KEGG

hsa:3456.

Organism-specific databases

H-InvDB

HIX0034856.

HGNC

HGNC:5434. IFNB1.

HPA

CAB009386.

MIM

147640. gene.

PharmGKB

PA29672.

GenAtlas

Search...

GeneCards

Search...

GeneLynx

Search...

Phylogenomic databases

HOGENOM

P01574.

HOVERGEN

P01574.

Gene expression databases

ArrayExpress

P01574.

CleanEx

HS_IFNB1.

GermOnline

ENSG00000171855. Homo sapiens.

Family and domain databases

InterPro

IPR012351. 4_helix_cytokine_core.
IPR015588. IFNb.
IPR000471. Interferon_abd.
[Graphical view]

Gene3D

G3DSA:1.20.1250.10. 4_helix_cytokine_core. 1 hit.

PANTHER

PTHR11691:SF7. IFNb. 1 hit.
PTHR11691. Interferon_abd. 1 hit.

Pfam

PF00143. Interferon. 1 hit.
[Graphical view]

PRINTS

PR00266. INTERFERONAB.

ProDom

PD000550. Interferon_abd. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00076. IFabd. 1 hit.
[Graphical view]

PROSITE

PS00252. INTERFERON_A_B_D. 1 hit.
[Graphical view]

BLOCKS

Search...

Other Resources

DrugBank

DB00060. Interferon beta-1a.
DB00068. Interferon beta-1b.

LinkHub

P01574.

SOURCE

Search...

ProtoNet

Search...

Entry information

Entry name

IFNB_HUMAN

Accession

Primary (citable) accession number: P01574

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	July 22, 2008
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)