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P01574 (IFNB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 163. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Interferon beta

Short name=IFN-beta
Alternative name(s):
Fibroblast interferon
Gene names
Name:IFNB1
Synonyms:IFB, IFNB
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length187 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has antiviral, antibacterial and anticancer activities.

Subunit structure

Monomer. Signals mostly via binding to a IFNAR1-IFNAR2 heterodimeric receptor, but can also function with IFNAR1 alone and independently of Jak-STAT pathways.

Subcellular location

Secreted.

Pharmaceutical use

Available under the names Avonex (Biogen), Betaseron (Berlex) and Rebif (Serono). Used in the treatment of multiple sclerosis (MS). Betaseron is a slightly modified form of IFNB1 with two residue substitutions.

Sequence similarities

Belongs to the alpha/beta interferon family.

Ontologies

Keywords
   Biological processAntiviral defense
   Cellular componentSecreted
   Coding sequence diversityPolymorphism
   DomainSignal
   Molecular functionCytokine
   PTMDisulfide bond
Glycoprotein
Phosphoprotein
   Technical term3D-structure
Complete proteome
Pharmaceutical
Reference proteome
Gene Ontology (GO)
   Biological_processB cell activation involved in immune response

Inferred from mutant phenotype PubMed 12932356. Source: UniProtKB

B cell differentiation

Inferred from Biological aspect of Ancestor. Source: RefGenome

B cell proliferation

Non-traceable author statement PubMed 3135963. Source: UniProtKB

T cell activation involved in immune response

Inferred from Biological aspect of Ancestor. Source: RefGenome

adaptive immune response

Inferred from electronic annotation. Source: Ensembl

blood coagulation

Traceable author statement. Source: Reactome

cell surface receptor signaling pathway

Traceable author statement PubMed 10640778. Source: ProtInc

cellular response to exogenous dsRNA

Traceable author statement PubMed 21266579. Source: BHF-UCL

cytokine-mediated signaling pathway

Inferred from Biological aspect of Ancestor. Source: RefGenome

defense response to bacterium

Inferred from electronic annotation. Source: Ensembl

defense response to virus

Traceable author statement PubMed 19416887. Source: BHF-UCL

humoral immune response

Inferred from Biological aspect of Ancestor. Source: RefGenome

innate immune response

Traceable author statement. Source: Reactome

natural killer cell activation

Non-traceable author statement PubMed 11987821. Source: UniProtKB

natural killer cell activation involved in immune response

Inferred from Biological aspect of Ancestor. Source: RefGenome

negative regulation of T cell differentiation

Inferred from direct assay PubMed 20554961. Source: UniProtKB

negative regulation of T-helper 2 cell cytokine production

Inferred from direct assay PubMed 20554961. Source: UniProtKB

negative regulation of viral genome replication

Inferred from direct assay PubMed 19008854. Source: BHF-UCL

positive regulation of apoptotic signaling pathway

Inferred from direct assay PubMed 10918594PubMed 11337497. Source: UniProtKB

positive regulation of innate immune response

Non-traceable author statement PubMed 11987821. Source: UniProtKB

positive regulation of peptidyl-serine phosphorylation of STAT protein

Inferred from direct assay PubMed 18035482. Source: BHF-UCL

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 18035482. Source: BHF-UCL

regulation of MHC class I biosynthetic process

Non-traceable author statement PubMed 8805651. Source: UniProtKB

regulation of type I interferon-mediated signaling pathway

Traceable author statement. Source: Reactome

response to exogenous dsRNA

Inferred from direct assay PubMed 21266579. Source: MGI

response to virus

Non-traceable author statement PubMed 12120696. Source: UniProtKB

type I interferon signaling pathway

Traceable author statement. Source: Reactome

   Cellular_componentextracellular region

Inferred by curator Ref.7. Source: UniProtKB

extracellular space

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Molecular_functioncytokine activity

Inferred from direct assay PubMed 19008854. Source: BHF-UCL

type I interferon receptor binding

Non-traceable author statement Ref.7. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121
Chain22 – 187166Interferon beta
PRO_0000016400

Amino acid modifications

Modified residue241Phosphotyrosine By similarity
Glycosylation1011N-linked (GlcNAc...)
Disulfide bond52 ↔ 162 Ref.13

Natural variations

Natural variant1621C → Y in clone PF526, loss of ability to form the essential disulfide bond, loss of antiviral activity.
VAR_004016
Natural variant1641W → C in a breast cancer sample; somatic mutation. Ref.16
VAR_036330

Secondary structure

................... 187
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P01574 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 0B013D4087723CEC

FASTA18722,294
        10         20         30         40         50         60 
MTNKCLLQIA LLLCFSTTAL SMSYNLLGFL QRSSNFQCQK LLWQLNGRLE YCLKDRMNFD 

        70         80         90        100        110        120 
IPEEIKQLQQ FQKEDAALTI YEMLQNIFAI FRQDSSSTGW NETIVENLLA NVYHQINHLK 

       130        140        150        160        170        180 
TVLEEKLEKE DFTRGKLMSS LHLKRYYGRI LHYLKAKEYS HCAWTIVRVE ILRNFYFINR 


LTGYLRN 

« Hide

References

« Hide 'large scale' references
[1]"Human fibroblast interferon gene lacks introns."
Lawn R.M., Adelman J., Franke A.E., Houck C.M., Gross M., Najarian R., Goeddel D.V.
Nucleic Acids Res. 9:1045-1052(1981) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Structure of a chromosomal gene for human interferon beta."
Ohno S., Taniguchi T.
Proc. Natl. Acad. Sci. U.S.A. 78:5305-5309(1981) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The nucleotide sequence of human fibroblast interferon cDNA."
Taniguchi T., Ohno S., Fujii-Kuriyama Y., Muramatsu M.
Gene 10:11-15(1980) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"Isolation and structure of a human fibroblast interferon gene."
Derynck R., Content J., Declercq E., Volckaert G., Tavernier J., Devos R., Fiers W.
Nature 285:542-547(1980) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"The complete amino acid sequence of human fibroblast interferon as deduced using synthetic oligodeoxyribonucleotide primers of reverse transcriptase."
Houghton M., Eaton M.A.W., Stewart A.G., Smith J.C., Doel S.M., Cartlin G.H., Lewis H.M., Patel T.P., Emtage J.S., Carey N.H., Porter A.G.
Nucleic Acids Res. 8:2885-2894(1980) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[6]"Synthesis of human fibroblast interferon by E. coli."
Goeddel D.V., Shepard H.M., Yelverton E., Leung D., Crea R., Sloma A., Pestka S.
Nucleic Acids Res. 8:4057-4074(1980) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[7]"On the relationship between human interferon alpha 1 and beta 1 genes."
May L.T., Sehgal P.B.
J. Interferon Res. 5:521-526(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[8]Livingston R.J., Shaffer T., McFarland I., Nguyen C.P., Stanaway I.B., Rajkumar N., Johnson E.J., da Ponte S.H., Willa H., Ahearn M.O., Bertucci C., Acklestad J., Carroll A., Swanson J., Gildersleeve H.I., Nickerson D.A.
Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[9]"Human protein factory for converting the transcriptome into an in vitro-expressed proteome."
Goshima N., Kawamura Y., Fukumoto A., Miura A., Honma R., Satoh R., Wakamatsu A., Yamamoto J., Kimura K., Nishikawa T., Andoh T., Iida Y., Ishikawa K., Ito E., Kagawa N., Kaminaga C., Kanehori K., Kawakami B. expand/collapse author list , Kenmochi K., Kimura R., Kobayashi M., Kuroita T., Kuwayama H., Maruyama Y., Matsuo K., Minami K., Mitsubori M., Mori M., Morishita R., Murase A., Nishikawa A., Nishikawa S., Okamoto T., Sakagami N., Sakamoto Y., Sasaki Y., Seki T., Sono S., Sugiyama A., Sumiya T., Takayama T., Takayama Y., Takeda H., Togashi T., Yahata K., Yamada H., Yanagisawa Y., Endo Y., Imamoto F., Kisu Y., Tanaka S., Isogai T., Imai J., Watanabe S., Nomura N.
Nat. Methods 5:1011-1017(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[10]Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[11]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[12]"The amino-terminal sequence of human fibroblast interferon as deduced from reverse transcripts obtained using synthetic oligonucleotide primers."
Houghton M., Stewart A.G., Doel S.M., Emtage J.S., Eaton M.A.W., Smith J.C., Patel T.P., Lewis H.M., Porter A.G., Birch J.R., Cartwright T., Carey N.H.
Nucleic Acids Res. 8:1913-1931(1980) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-68.
[13]"Assignment of the disulphide bonds of leukocyte interferon."
Wetzel R.
Nature 289:606-607(1981) [PubMed] [Europe PMC] [Abstract]
Cited for: DISULFIDE BOND.
[14]"A single amino acid change in IFN-beta1 abolishes its antiviral activity."
Shepard H.M., Leung D., Stebbing N., Goeddel D.V.
Nature 294:563-565(1981) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 71-187 (VARIANT CLONE PF526).
[15]"The crystal structure of human interferon beta at 2.2-A resolution."
Karpusas M., Nolte M., Benton C.B., Meier W., Lipscomb W.N., Goelz S.
Proc. Natl. Acad. Sci. U.S.A. 94:11813-11818(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
[16]"The consensus coding sequences of human breast and colorectal cancers."
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. expand/collapse author list , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.
Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] CYS-164.
+Additional computationally mapped references.

Web resources

Avonex

Clinical information on Avonex

Betaseron

Clinical information on Betaseron

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
V00534 Genomic DNA. Translation: CAA23795.1.
V00535 Genomic DNA. Translation: CAA23796.1.
V00546 mRNA. Translation: CAA23807.1.
V00547 mRNA. Translation: CAA23808.1.
M28622 mRNA. Translation: AAA36040.1.
EF064725 Genomic DNA. Translation: ABK41908.1.
AB451323 mRNA. Translation: BAG70137.1.
AB451452 mRNA. Translation: BAG70266.1.
CH471071 Genomic DNA. Translation: EAW58625.1.
BC069314 mRNA. Translation: AAH69314.1.
BC096150 mRNA. Translation: AAH96150.1.
BC096151 mRNA. Translation: AAH96151.1.
BC096152 mRNA. Translation: AAH96152.1.
BC096153 mRNA. Translation: AAH96153.1.
CCDSCCDS6495.1.
PIRIVHUB1. A93721.
RefSeqNP_002167.1. NM_002176.2.
UniGeneHs.93177.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1AU1X-ray2.20A/B22-187[»]
ProteinModelPortalP01574.
SMRP01574. Positions 22-187.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid109678. 1 interaction.
DIPDIP-6018N.
STRING9606.ENSP00000369581.

Chemistry

DrugBankDB00060. Interferon beta-1a.
DB00068. Interferon beta-1b.

Protein family/group databases

Allergome9875. Hom s IFN beta.

PTM databases

PhosphoSiteP01574.

Polymorphism databases

DMDM124469.

Proteomic databases

PaxDbP01574.
PRIDEP01574.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000380232; ENSP00000369581; ENSG00000171855.
GeneID3456.
KEGGhsa:3456.
UCSCuc003zok.3. human.

Organism-specific databases

CTD3456.
GeneCardsGC09M021077.
HGNCHGNC:5434. IFNB1.
HPACAB009386.
MIM147640. gene.
neXtProtNX_P01574.
PharmGKBPA29672.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG43895.
HOGENOMHOG000230501.
HOVERGENHBG052086.
InParanoidP01574.
KOK05415.
OMANGRLEYC.
OrthoDBEOG7J9VRD.
PhylomeDBP01574.
TreeFamTF336177.

Enzyme and pathway databases

ReactomeREACT_604. Hemostasis.
REACT_6900. Immune System.
SignaLinkP01574.

Gene expression databases

ArrayExpressP01574.
CleanExHS_IFNB1.
GenevestigatorP01574.

Family and domain databases

Gene3D1.20.1250.10. 1 hit.
InterProIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR000471. Interferon_alpha/beta/delta.
IPR015588. Interferon_beta.
[Graphical view]
PANTHERPTHR11691. PTHR11691. 1 hit.
PTHR11691:SF7. PTHR11691:SF7. 1 hit.
PfamPF00143. Interferon. 1 hit.
[Graphical view]
PRINTSPR00266. INTERFERONAB.
SMARTSM00076. IFabd. 1 hit.
[Graphical view]
SUPFAMSSF47266. SSF47266. 1 hit.
PROSITEPS00252. INTERFERON_A_B_D. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP01574.
GeneWikiIFNB1.
GenomeRNAi3456.
NextBio13618.
PMAP-CutDBP01574.
PROP01574.
SOURCESearch...

Entry information

Entry nameIFNB_HUMAN
AccessionPrimary (citable) accession number: P01574
Secondary accession number(s): Q5VWC9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: July 9, 2014
This is version 163 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 9

Human chromosome 9: entries, gene names and cross-references to MIM