ID   IFNA1_MOUSE             Reviewed;         189 AA.
AC   P01572; Q7M0A3; Q810G7;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   08-FEB-2011, sequence version 2.
DT   27-MAR-2024, entry version 169.
DE   RecName: Full=Interferon alpha-1;
DE            Short=IFN-alpha-1;
DE   Flags: Precursor;
GN   Name=Ifna1;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=6188104; DOI=10.1093/nar/11.3.555;
RA   Shaw G.D., Boll W., Taira H., Mantei N., Lengyel P., Weissmann C.;
RT   "Structure and expression of cloned murine IFN-alpha genes.";
RL   Nucleic Acids Res. 11:555-573(1983).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2987811; DOI=10.1093/nar/13.3.805;
RA   Kelly K.A., Pitha P.M.;
RT   "Characterization of a mouse interferon gene locus I. Isolation of a
RT   cluster of four alpha interferon genes.";
RL   Nucleic Acids Res. 13:805-823(1985).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND GLYCOSYLATION.
RC   STRAIN=C57BL/6J;
RX   PubMed=15254193; DOI=10.1128/jvi.78.15.8219-8228.2004;
RA   van Pesch V., Lanaya H., Renauld J.C., Michiels T.;
RT   "Characterization of the murine alpha interferon gene family.";
RL   J. Virol. 78:8219-8228(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   PROTEIN SEQUENCE OF 24-51; 124-146 AND 175-181.
RX   PubMed=9244179; DOI=10.1016/0167-4889(95)00159-x;
RA   Beare D., Learmonth M., Wells V., Aitken A., Mallucci L.;
RT   "Characterisation and antiproliferative activity of an alpha-type murine
RT   interferon from embryonic fibroblasts.";
RL   Biochim. Biophys. Acta 1310:81-85(1996).
CC   -!- FUNCTION: Produced by macrophages, IFN-alpha have antiviral activities.
CC       Interferon stimulates the production of two enzymes: a protein kinase
CC       and an oligoadenylate synthetase. {ECO:0000269|PubMed:15254193}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- PTM: Glycosylated. {ECO:0000269|PubMed:15254193}.
CC   -!- SIMILARITY: Belongs to the alpha/beta interferon family. {ECO:0000305}.
CC   -!- CAUTION: Was named interferon alpha-E (embryonic) based on peptide
CC       sequencing (PubMed:9244179). The differences found may be sequencing
CC       erros and have not been confirmed by other studies.
CC       {ECO:0000305|PubMed:9244179}.
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DR   EMBL; X01974; CAA26006.1; -; Genomic_DNA.
DR   EMBL; AY225950; AAO63592.1; -; Genomic_DNA.
DR   EMBL; BX530016; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH466527; EDL30958.1; -; Genomic_DNA.
DR   CCDS; CCDS18347.1; -.
DR   PIR; A01836; IVMSA1.
DR   PIR; S62682; S62682.
DR   RefSeq; NP_034632.2; NM_010502.2.
DR   AlphaFoldDB; P01572; -.
DR   SMR; P01572; -.
DR   STRING; 10090.ENSMUSP00000092580; -.
DR   GlyCosmos; P01572; 1 site, No reported glycans.
DR   GlyGen; P01572; 1 site.
DR   iPTMnet; P01572; -.
DR   PhosphoSitePlus; P01572; -.
DR   PaxDb; 10090-ENSMUSP00000092580; -.
DR   ABCD; P01572; 1 sequenced antibody.
DR   DNASU; 15962; -.
DR   Ensembl; ENSMUST00000094972.2; ENSMUSP00000092580.2; ENSMUSG00000095498.2.
DR   GeneID; 15962; -.
DR   KEGG; mmu:15962; -.
DR   UCSC; uc008toc.1; mouse.
DR   AGR; MGI:107668; -.
DR   CTD; 3439; -.
DR   MGI; MGI:107668; Ifna1.
DR   VEuPathDB; HostDB:ENSMUSG00000095498; -.
DR   eggNOG; ENOG502SQAC; Eukaryota.
DR   GeneTree; ENSGT01000000214430; -.
DR   HOGENOM; CLU_109427_0_0_1; -.
DR   InParanoid; P01572; -.
DR   OMA; RRTLMIM; -.
DR   OrthoDB; 5358931at2759; -.
DR   PhylomeDB; P01572; -.
DR   TreeFam; TF336177; -.
DR   Reactome; R-MMU-909733; Interferon alpha/beta signaling.
DR   Reactome; R-MMU-912694; Regulation of IFNA/IFNB signaling.
DR   SABIO-RK; P01572; -.
DR   BioGRID-ORCS; 15962; 9 hits in 43 CRISPR screens.
DR   PRO; PR:P01572; -.
DR   Proteomes; UP000000589; Chromosome 4.
DR   RNAct; P01572; Protein.
DR   GO; GO:0005576; C:extracellular region; TAS:Reactome.
DR   GO; GO:0005615; C:extracellular space; IDA:CAFA.
DR   GO; GO:0005125; F:cytokine activity; IDA:MGI.
DR   GO; GO:0005132; F:type I interferon receptor binding; IBA:GO_Central.
DR   GO; GO:0002250; P:adaptive immune response; IBA:GO_Central.
DR   GO; GO:0030183; P:B cell differentiation; IBA:GO_Central.
DR   GO; GO:0042100; P:B cell proliferation; IBA:GO_Central.
DR   GO; GO:0019221; P:cytokine-mediated signaling pathway; IBA:GO_Central.
DR   GO; GO:0042742; P:defense response to bacterium; IDA:MGI.
DR   GO; GO:0051607; P:defense response to virus; IDA:MGI.
DR   GO; GO:0006959; P:humoral immune response; IBA:GO_Central.
DR   GO; GO:0002323; P:natural killer cell activation involved in immune response; IDA:MGI.
DR   GO; GO:0033141; P:positive regulation of peptidyl-serine phosphorylation of STAT protein; IBA:GO_Central.
DR   GO; GO:0050691; P:regulation of defense response to virus by host; IDA:CAFA.
DR   GO; GO:0043330; P:response to exogenous dsRNA; IBA:GO_Central.
DR   GO; GO:0002286; P:T cell activation involved in immune response; IDA:MGI.
DR   GO; GO:0060337; P:type I interferon-mediated signaling pathway; IDA:CAFA.
DR   CDD; cd00095; IFab; 1.
DR   Gene3D; 1.20.1250.10; -; 1.
DR   InterPro; IPR009079; 4_helix_cytokine-like_core.
DR   InterPro; IPR000471; Interferon_alpha/beta/delta.
DR   PANTHER; PTHR11691:SF60; INTERFERON ALPHA-5; 1.
DR   PANTHER; PTHR11691; TYPE I INTERFERON; 1.
DR   Pfam; PF00143; Interferon; 1.
DR   PRINTS; PR00266; INTERFERONAB.
DR   SMART; SM00076; IFabd; 1.
DR   SUPFAM; SSF47266; 4-helical cytokines; 1.
DR   PROSITE; PS00252; INTERFERON_A_B_D; 1.
DR   Genevisible; P01572; MM.
PE   1: Evidence at protein level;
KW   Antiviral defense; Cytokine; Direct protein sequencing; Disulfide bond;
KW   Glycoprotein; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000269|PubMed:9244179"
FT   CHAIN           24..189
FT                   /note="Interferon alpha-1"
FT                   /id="PRO_0000016375"
FT   CARBOHYD        101
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000305|PubMed:15254193"
FT   DISULFID        24..122
FT                   /evidence="ECO:0000250"
FT   DISULFID        52..162
FT                   /evidence="ECO:0000250"
FT   CONFLICT        14
FT                   /note="L -> M (in Ref. 1; no nucleotide entry and 2;
FT                   CAA26006)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        102
FT                   /note="T -> A (in Ref. 1; no nucleotide entry and 2;
FT                   CAA26006)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        126
FT                   /note="Q -> E (in Ref. 6; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        132
FT                   /note="F -> P (in Ref. 6; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        139
FT                   /note="A -> Y (in Ref. 6; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        145
FT                   /note="K -> T (in Ref. 6; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        175
FT                   /note="L -> M (in Ref. 6; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        180..181
FT                   /note="NV -> KL (in Ref. 6; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   189 AA;  21646 MW;  D6F4BC96DCE7D67E CRC64;
     MARLCAFLMV LAVLSYWPTC SLGCDLPQTH NLRNKRALTL LVQMRRLSPL SCLKDRKDFG
     FPQEKVDAQQ IKKAQAIPVL SELTQQILNI FTSKDSSAAW NTTLLDSFCN DLHQQLNDLQ
     GCLMQQVGVQ EFPLTQEDAL LAVRKYFHRI TVYLREKKHS PCAWEVVRAE VWRALSSSAN
     VLGRLREEK
//