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P01563 (IFNA2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 148. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Interferon alpha-2

Short name=IFN-alpha-2
Alternative name(s):
Interferon alpha-A
Short name=LeIF A
Gene names
Name:IFNA2
Synonyms:IFNA2A, IFNA2B, IFNA2C
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length188 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Produced by macrophages, IFN-alpha have antiviral activities.

Subunit structure

Interacts with IFNAR2. Ref.21 Ref.22

Subcellular location

Secreted.

Polymorphism

Three forms exist; alpha-2a (shown here), alpha-2b and alpha-2c.

Pharmaceutical use

Available under the names Roferon-A (Roche) or Intron-A (Schering-Plough). Used as an anticancer drug for its antiproliferative activity.

Sequence similarities

Belongs to the alpha/beta interferon family.

Ontologies

Keywords
   Biological processAntiviral defense
   Cellular componentSecreted
   Coding sequence diversityPolymorphism
   DomainSignal
   Molecular functionCytokine
   PTMDisulfide bond
Glycoprotein
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Pharmaceutical
Reference proteome
Gene Ontology (GO)
   Biological_processapoptotic process

Traceable author statement PubMed 10477584. Source: ProtInc

blood coagulation

Traceable author statement. Source: Reactome

cell surface receptor signaling pathway

Traceable author statement Ref.11. Source: ProtInc

cell-cell signaling

Traceable author statement PubMed 10477584. Source: ProtInc

cytokine-mediated signaling pathway

Traceable author statement. Source: Reactome

defense response to virus

Inferred from direct assay PubMed 22783022. Source: BHF-UCL

inflammatory response

Traceable author statement PubMed 10477584. Source: ProtInc

innate immune response

Traceable author statement. Source: Reactome

negative regulation of T cell differentiation

Inferred from direct assay PubMed 20554961. Source: UniProtKB

negative regulation of T-helper 2 cell cytokine production

Inferred from direct assay PubMed 20554961. Source: UniProtKB

negative regulation of gene expression

Inferred from direct assay PubMed 20554961. Source: UniProtKB

negative regulation of interleukin-13 secretion

Inferred from direct assay PubMed 20554961. Source: UniProtKB

negative regulation of interleukin-5 secretion

Inferred from direct assay PubMed 20554961. Source: UniProtKB

negative regulation of transcription, DNA-templated

Inferred from direct assay PubMed 20554961. Source: UniProtKB

negative regulation of viral entry into host cell

Inferred from direct assay PubMed 22783022. Source: BHF-UCL

positive regulation of tyrosine phosphorylation of Stat3 protein

Inferred from direct assay PubMed 22783022. Source: BHF-UCL

regulation of type I interferon-mediated signaling pathway

Traceable author statement. Source: Reactome

type I interferon signaling pathway

Traceable author statement. Source: Reactome

   Cellular_componentextracellular region

Traceable author statement. Source: Reactome

extracellular space

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionprotein binding

Inferred from physical interaction PubMed 7759950. Source: UniProtKB

type I interferon receptor binding

Traceable author statement Ref.11. Source: ProtInc

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Ref.13 Ref.14
Chain24 – 188165Interferon alpha-2
PRO_0000016360

Amino acid modifications

Glycosylation1291O-linked (GalNAc...) Ref.16
CAR_000049
Disulfide bond24 ↔ 121 Ref.15 Ref.21 Ref.22
Disulfide bond52 ↔ 161 Ref.15 Ref.21 Ref.22

Natural variations

Natural variant61A → D.
Corresponds to variant rs35971916 [ dbSNP | Ensembl ].
VAR_055972
Natural variant461K → R in alpha-2B and alpha-2C. Ref.6
Corresponds to variant rs1061959 [ dbSNP | Ensembl ].
VAR_004012
Natural variant571H → R in alpha-2C.
VAR_013001
Natural variant1771S → L in a breast cancer sample; somatic mutation. Ref.23
VAR_036329

Experimental info

Sequence conflict851Q → L in AEX60803. Ref.6
Sequence conflict931K → M in AEX60802. Ref.6

Secondary structure

........................... 188
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P01563 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 101DD21D394CBF97

FASTA18821,550
        10         20         30         40         50         60 
MALTFALLVA LLVLSCKSSC SVGCDLPQTH SLGSRRTLML LAQMRKISLF SCLKDRHDFG 

        70         80         90        100        110        120 
FPQEEFGNQF QKAETIPVLH EMIQQIFNLF STKDSSAAWD ETLLDKFYTE LYQQLNDLEA 

       130        140        150        160        170        180 
CVIQGVGVTE TPLMKEDSIL AVRKYFQRIT LYLKEKKYSP CAWEVVRAEI MRSFSLSTNL 


QESLRSKE 

« Hide

References

« Hide 'large scale' references
[1]"Human leukocyte interferon produced by E. coli is biologically active."
Goeddel D.V., Yelverton E., Ullrich A., Heyneker H.L., Miozzari G., Holmes W., Seeburg P.H., Dull T.J., May L., Stebbing N., Crea R., Maeda S., McCandliss R., Sloma A., Tabor J.M., Gross M., Familletti P.C., Pestka S.
Nature 287:411-416(1980) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]"The structure of eight distinct cloned human leukocyte interferon cDNAs."
Goeddel D.V., Leung D.W., Dull T.J., Gross M., Lawn R.M., McCandliss R., Seeburg P.H., Ullrich A., Yelverton E., Gray P.W.
Nature 290:20-26(1981) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[3]"DNA sequence of a major human leukocyte interferon gene."
Lawn R.M., Gross M., Houck C.M., Franke A.E., Gray P.V., Goeddel D.V.
Proc. Natl. Acad. Sci. U.S.A. 78:5435-5439(1981) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[4]"Cloning of human leukocyte interferon cDNA and a strategy for its production in E. coli."
Oliver G., Balbas P., Valle F., Soberon X., Bolivar F.
Rev. Latinoam. Microbiol. 27:141-150(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Bone marrow tumor.
[5]"A defective retroviral vector encoding human interferon alpha 2 can transduce human leukemic cell lines."
Austruy E., Bagnis C., Carbuccia N., Maroc C., Birg F., Dubreuil P., Mannoni P., Chabannon C.
Cancer Gene Ther. 5:247-256(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Placenta.
[6]"Heterologous expression, immunochemical and computational analysis of recombinant human interferon alpha 2b."
Gull I., Samra Z.Q., Aslam M.S., Athar M.A.
Springerplus 2:264-264(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ARG-46.
[7]Hanif K., Noor S., Naveed Y., Bashir B., Hussain T., Kanwal N.
Submitted (OCT-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[8]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[9]Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[10]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[11]"At least three human type alpha interferons: structure of alpha 2."
Streuli M., Nagata S., Weissmann C.
Science 209:1343-1347(1980) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 7-188.
[12]"Formation of genes coding for hybrid proteins by recombination between related, cloned genes in E. coli."
Weber H., Weissmann C.
Nucleic Acids Res. 11:5661-5669(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 24-188.
[13]"A family of structural genes for human lymphoblastoid (leukocyte-type) interferon."
Allen G., Fantes K.H.
Nature 287:408-411(1980) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 24-112 AND 136-188.
[14]"Identification of nine interferon-alpha subtypes produced by Sendai virus-induced human peripheral blood leucocytes."
Nyman T.A., Toeloe H., Parkkinen J., Kalkkinen N.
Biochem. J. 329:295-302(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 24-58.
[15]"Assignment of the disulphide bonds of leukocyte interferon."
Wetzel R.
Nature 289:606-607(1981) [PubMed] [Europe PMC] [Abstract]
Cited for: DISULFIDE BONDS.
[16]"Natural human interferon-alpha 2 is O-glycosylated."
Adolf G.R., Kalsner I., Ahorn H., Maurer-Fogy I., Cantell K.
Biochem. J. 276:511-518(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION AT THR-129, ALLELES ALPHA-2B AND ALPHA-2C.
[17]"Interferon-alpha 2 variants in the human genome."
Lee N., Ni D., Brissette R., Chou M., Hussain M., Gill D.S., Liao M.-J., Testa D.
J. Interferon Cytokine Res. 15:341-349(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: POLYMORPHISM.
[18]"A homology model of human interferon alpha-2."
Murgolo N.J., Windsor W.T., Hruza A., Reichert P., Tsarbopoulos A., Baldwin S., Huang E., Pramanik B., Ealick S., Trotta P.P.
Proteins 17:62-74(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: 3D-STRUCTURE MODELING.
[19]"Zinc mediated dimer of human interferon-alpha 2b revealed by X-ray crystallography."
Radhakrishnan R., Walter L.J., Hruza A., Reichert P., Trotta P.P., Nagabhushan T.L., Walter M.R.
Structure 4:1453-1463(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS).
[20]"The three-dimensional high resolution structure of human interferon alpha-2a determined by heteronuclear NMR spectroscopy in solution."
Klaus W., Gsell B., Labhardt A.M., Wipf B., Senn H.
J. Mol. Biol. 274:661-675(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR.
[21]"Determination of the human type I interferon receptor binding site on human interferon-alpha2 by cross saturation and an NMR-based model of the complex."
Quadt-Akabayov S.R., Chill J.H., Levy R., Kessler N., Anglister J.
Protein Sci. 15:2656-2668(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 24-188 IN COMPLEX WITH IFNAR2, SUBUNIT, DISULFIDE BONDS.
[22]"Intermolecular interactions in a 44 kDa interferon-receptor complex detected by asymmetric reverse-protonation and two-dimensional NOESY."
Nudelman I., Akabayov S.R., Schnur E., Biron Z., Levy R., Xu Y., Yang D., Anglister J.
Biochemistry 49:5117-5133(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 24-188 IN COMPLEX WITH IFNAR2, SUBUNIT, DISULFIDE BONDS.
[23]"The consensus coding sequences of human breast and colorectal cancers."
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. expand/collapse author list , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.
Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] LEU-177.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J00207 Genomic DNA. Translation: AAB59402.1.
V00544 mRNA. Translation: CAA23805.1.
V00548 mRNA. Translation: CAA23809.1.
V00549 mRNA. Translation: CAA23810.1.
Y11834 Genomic DNA. Translation: CAA72532.1.
JN591568 mRNA. Translation: AEX60802.1.
JN591569 mRNA. Translation: AEX60803.1.
JN591570 mRNA. Translation: AEX60804.1.
JN848522 mRNA. Translation: AET86951.1.
CR541921 mRNA. Translation: CAG46719.1.
CH471071 Genomic DNA. Translation: EAW58611.1.
BC074936 mRNA. Translation: AAH74936.1.
BC074937 mRNA. Translation: AAH74937.1.
BC104163 mRNA. Translation: AAI04164.1.
BC104164 mRNA. Translation: AAI04165.1.
M54886 mRNA. Translation: AAA59181.1.
M29883 Genomic DNA. Translation: AAA52715.1.
CCDSCCDS6506.1.
PIRIVHUA2. A93234.
I78570.
RefSeqNP_000596.2. NM_000605.3.
UniGeneHs.211575.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1ITFNMR-A24-188[»]
1RH2X-ray2.90A/B/C/D/E/F24-188[»]
2HIEmodel-A24-188[»]
2HYMNMR-B24-188[»]
2KZ1NMR-A24-188[»]
2LAGNMR-A24-188[»]
2LMSNMR-A24-188[»]
3S9DX-ray2.00A/C24-188[»]
3SE3X-ray4.00B24-188[»]
ProteinModelPortalP01563.
SMRP01563. Positions 24-188.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid109663. 1 interaction.
DIPDIP-3784N.
DIP-481N.
IntActP01563. 1 interaction.
STRING9606.ENSP00000369554.

Chemistry

DrugBankDB00034. Interferon Alfa-2a, Recombinant.
DB00105. Interferon Alfa-2b, Recombinant.
DB00011. Interferon alfa-n1.
DB00008. Peginterferon alfa-2a.
DB00022. Peginterferon alfa-2b.

Protein family/group databases

Allergome9876. Hom s IFN alpha.

PTM databases

PhosphoSiteP01563.
UniCarbKBP01563.

Polymorphism databases

DMDM124449.

Proteomic databases

PaxDbP01563.
PRIDEP01563.

Protocols and materials databases

DNASU3440.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000380206; ENSP00000369554; ENSG00000188379.
GeneID3440.
KEGGhsa:3440.

Organism-specific databases

CTD3440.
GeneCardsGC09M021374.
H-InvDBHIX0034810.
HGNCHGNC:5423. IFNA2.
HPAHPA047557.
MIM147562. gene.
neXtProtNX_P01563.
PharmGKBPA29662.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG246451.
HOGENOMHOG000230500.
HOVERGENHBG052086.
InParanoidP01563.
KOK05414.
OrthoDBEOG7M98HV.
PhylomeDBP01563.
TreeFamTF336177.

Enzyme and pathway databases

ReactomeREACT_604. Hemostasis.
REACT_6900. Immune System.

Gene expression databases

CleanExHS_IFNA2.
GenevestigatorP01563.

Family and domain databases

Gene3D1.20.1250.10. 1 hit.
InterProIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR000471. Interferon_alpha/beta/delta.
[Graphical view]
PANTHERPTHR11691. PTHR11691. 1 hit.
PfamPF00143. Interferon. 1 hit.
[Graphical view]
PRINTSPR00266. INTERFERONAB.
SMARTSM00076. IFabd. 1 hit.
[Graphical view]
SUPFAMSSF47266. SSF47266. 1 hit.
PROSITEPS00252. INTERFERON_A_B_D. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP01563.
GeneWikiIFNA2.
GenomeRNAi3440.
NextBio13556.
PROP01563.
SOURCESearch...

Entry information

Entry nameIFNA2_HUMAN
AccessionPrimary (citable) accession number: P01563
Secondary accession number(s): H2DF54 expand/collapse secondary AC list , H2DF55, P01564, Q14606, Q6DJX8, Q96KI6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: July 9, 2014
This is version 148 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 9

Human chromosome 9: entries, gene names and cross-references to MIM