Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Interferon alpha-2

Gene

IFNA2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Produced by macrophages, IFN-alpha have antiviral activities.

GO - Molecular functioni

  • cytokine activity Source: GO_Central
  • type I interferon receptor binding Source: ProtInc

GO - Biological processi

  • apoptotic process Source: ProtInc
  • B cell differentiation Source: GO_Central
  • B cell proliferation Source: GO_Central
  • blood coagulation Source: Reactome
  • cell-cell signaling Source: ProtInc
  • cell surface receptor signaling pathway Source: ProtInc
  • cytokine-mediated signaling pathway Source: GO_Central
  • defense response to virus Source: BHF-UCL
  • humoral immune response Source: GO_Central
  • inflammatory response Source: ProtInc
  • innate immune response Source: GO_Central
  • natural killer cell activation involved in immune response Source: GO_Central
  • negative regulation of gene expression Source: UniProtKB
  • negative regulation of interleukin-13 secretion Source: UniProtKB
  • negative regulation of interleukin-5 secretion Source: UniProtKB
  • negative regulation of T cell differentiation Source: UniProtKB
  • negative regulation of T-helper 2 cell cytokine production Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • negative regulation of viral entry into host cell Source: BHF-UCL
  • positive regulation of peptidyl-serine phosphorylation of STAT protein Source: GO_Central
  • positive regulation of phosphorylation Source: AgBase
  • positive regulation of transcription, DNA-templated Source: AgBase
  • positive regulation of tyrosine phosphorylation of Stat3 protein Source: BHF-UCL
  • regulation of type I interferon-mediated signaling pathway Source: Reactome
  • response to exogenous dsRNA Source: GO_Central
  • T cell activation involved in immune response Source: GO_Central
  • type I interferon signaling pathway Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Cytokine

Keywords - Biological processi

Antiviral defense

Enzyme and pathway databases

BioCyciZFISH:G66-31254-MONOMER.
ReactomeiR-HSA-909733. Interferon alpha/beta signaling.
R-HSA-912694. Regulation of IFNA signaling.
R-HSA-933541. TRAF6 mediated IRF7 activation.
R-HSA-983231. Factors involved in megakaryocyte development and platelet production.
SIGNORiP01563.

Names & Taxonomyi

Protein namesi
Recommended name:
Interferon alpha-2
Short name:
IFN-alpha-2
Alternative name(s):
Interferon alpha-A
Short name:
LeIF A
Gene namesi
Name:IFNA2
Synonyms:IFNA2A, IFNA2B, IFNA2C
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

HGNCiHGNC:5423. IFNA2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Pharmaceutical usei

Available under the names Roferon-A (Roche) or Intron-A (Schering-Plough). Used as an anticancer drug for its antiproliferative activity.

Organism-specific databases

DisGeNETi3440.
PharmGKBiPA29662.

Protein family/group databases

Allergomei9876. Hom s IFN alpha.

Polymorphism and mutation databases

BioMutaiIFNA2.
DMDMi124449.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 232 PublicationsAdd BLAST23
ChainiPRO_000001636024 – 188Interferon alpha-2Add BLAST165

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi24 ↔ 1211 Publication
Disulfide bondi52 ↔ 1611 Publication
GlycosylationiCAR_000049129O-linked (GalNAc...)1 Publication1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiP01563.
PaxDbiP01563.
PeptideAtlasiP01563.
PRIDEiP01563.

PTM databases

iPTMnetiP01563.
PhosphoSitePlusiP01563.
UniCarbKBiP01563.

Expressioni

Gene expression databases

CleanExiHS_IFNA2.
GenevisibleiP01563. HS.

Organism-specific databases

HPAiHPA047557.

Interactioni

Subunit structurei

Interacts with IFNAR2.2 Publications

GO - Molecular functioni

  • cytokine activity Source: GO_Central
  • type I interferon receptor binding Source: ProtInc

Protein-protein interaction databases

BioGridi109663. 8 interactors.
DIPiDIP-3784N.
DIP-481N.
IntActiP01563. 1 interactor.
STRINGi9606.ENSP00000369554.

Structurei

Secondary structure

1188
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi27 – 30Combined sources4
Helixi32 – 44Combined sources13
Helixi50 – 55Combined sources6
Helixi63 – 65Combined sources3
Beta strandi66 – 70Combined sources5
Turni72 – 74Combined sources3
Helixi76 – 89Combined sources14
Helixi93 – 98Combined sources6
Helixi101 – 120Combined sources20
Beta strandi126 – 129Combined sources4
Turni132 – 135Combined sources4
Helixi138 – 155Combined sources18
Turni156 – 158Combined sources3
Helixi160 – 177Combined sources18
Beta strandi180 – 182Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ITFNMR-A24-188[»]
1RH2X-ray2.90A/B/C/D/E/F24-188[»]
2HIEmodel-A24-188[»]
2HYMNMR-B24-188[»]
2KZ1NMR-A24-188[»]
2LAGNMR-A24-188[»]
2LMSNMR-A24-188[»]
3S9DX-ray2.00A/C24-188[»]
3SE3X-ray4.00B24-188[»]
4YPGX-ray3.00C/D24-182[»]
4Z5RX-ray3.00D/E/F/G/H/I/N/X24-188[»]
ProteinModelPortaliP01563.
SMRiP01563.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP01563.

Family & Domainsi

Sequence similaritiesi

Belongs to the alpha/beta interferon family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410J735. Eukaryota.
ENOG410ZH91. LUCA.
HOGENOMiHOG000230500.
HOVERGENiHBG052086.
InParanoidiP01563.
KOiK05414.
OrthoDBiEOG091G0MJ6.
PhylomeDBiP01563.
TreeFamiTF336177.

Family and domain databases

CDDicd00095. IFab. 1 hit.
Gene3Di1.20.1250.10. 1 hit.
InterProiIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR000471. Interferon_alpha/beta/delta.
[Graphical view]
PANTHERiPTHR11691. PTHR11691. 1 hit.
PfamiPF00143. Interferon. 1 hit.
[Graphical view]
PRINTSiPR00266. INTERFERONAB.
SMARTiSM00076. IFabd. 1 hit.
[Graphical view]
SUPFAMiSSF47266. SSF47266. 1 hit.
PROSITEiPS00252. INTERFERON_A_B_D. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01563-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALTFALLVA LLVLSCKSSC SVGCDLPQTH SLGSRRTLML LAQMRKISLF
60 70 80 90 100
SCLKDRHDFG FPQEEFGNQF QKAETIPVLH EMIQQIFNLF STKDSSAAWD
110 120 130 140 150
ETLLDKFYTE LYQQLNDLEA CVIQGVGVTE TPLMKEDSIL AVRKYFQRIT
160 170 180
LYLKEKKYSP CAWEVVRAEI MRSFSLSTNL QESLRSKE
Length:188
Mass (Da):21,550
Last modified:July 21, 1986 - v1
Checksum:i101DD21D394CBF97
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti85Q → L in AEX60803 (PubMed:23875128).Curated1
Sequence conflicti93K → M in AEX60802 (PubMed:23875128).Curated1

Polymorphismi

Three forms exist; alpha-2a (shown here), alpha-2b and alpha-2c (PubMed:7627809).1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0559726A → D.Corresponds to variant rs35971916dbSNPEnsembl.1
Natural variantiVAR_00401246K → R in alpha-2B and alpha-2C. 1 PublicationCorresponds to variant rs1061959dbSNPEnsembl.1
Natural variantiVAR_01300157H → R in alpha-2C. 1
Natural variantiVAR_036329177S → L in a breast cancer sample; somatic mutation. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J00207 Genomic DNA. Translation: AAB59402.1.
V00544 mRNA. Translation: CAA23805.1.
V00548 mRNA. Translation: CAA23809.1.
V00549 mRNA. Translation: CAA23810.1.
Y11834 Genomic DNA. Translation: CAA72532.1.
JN591568 mRNA. Translation: AEX60802.1.
JN591569 mRNA. Translation: AEX60803.1.
JN591570 mRNA. Translation: AEX60804.1.
JN848522 mRNA. Translation: AET86951.1.
CR541921 mRNA. Translation: CAG46719.1.
CH471071 Genomic DNA. Translation: EAW58611.1.
BC074936 mRNA. Translation: AAH74936.1.
BC074937 mRNA. Translation: AAH74937.1.
BC104163 mRNA. Translation: AAI04164.1.
BC104164 mRNA. Translation: AAI04165.1.
M54886 mRNA. Translation: AAA59181.1.
M29883 Genomic DNA. Translation: AAA52715.1.
CCDSiCCDS6506.1.
PIRiA93234. IVHUA2.
I78570.
RefSeqiNP_000596.2. NM_000605.3.
UniGeneiHs.211575.

Genome annotation databases

EnsembliENST00000380206; ENSP00000369554; ENSG00000188379.
GeneIDi3440.
KEGGihsa:3440.
UCSCiuc003zpb.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J00207 Genomic DNA. Translation: AAB59402.1.
V00544 mRNA. Translation: CAA23805.1.
V00548 mRNA. Translation: CAA23809.1.
V00549 mRNA. Translation: CAA23810.1.
Y11834 Genomic DNA. Translation: CAA72532.1.
JN591568 mRNA. Translation: AEX60802.1.
JN591569 mRNA. Translation: AEX60803.1.
JN591570 mRNA. Translation: AEX60804.1.
JN848522 mRNA. Translation: AET86951.1.
CR541921 mRNA. Translation: CAG46719.1.
CH471071 Genomic DNA. Translation: EAW58611.1.
BC074936 mRNA. Translation: AAH74936.1.
BC074937 mRNA. Translation: AAH74937.1.
BC104163 mRNA. Translation: AAI04164.1.
BC104164 mRNA. Translation: AAI04165.1.
M54886 mRNA. Translation: AAA59181.1.
M29883 Genomic DNA. Translation: AAA52715.1.
CCDSiCCDS6506.1.
PIRiA93234. IVHUA2.
I78570.
RefSeqiNP_000596.2. NM_000605.3.
UniGeneiHs.211575.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ITFNMR-A24-188[»]
1RH2X-ray2.90A/B/C/D/E/F24-188[»]
2HIEmodel-A24-188[»]
2HYMNMR-B24-188[»]
2KZ1NMR-A24-188[»]
2LAGNMR-A24-188[»]
2LMSNMR-A24-188[»]
3S9DX-ray2.00A/C24-188[»]
3SE3X-ray4.00B24-188[»]
4YPGX-ray3.00C/D24-182[»]
4Z5RX-ray3.00D/E/F/G/H/I/N/X24-188[»]
ProteinModelPortaliP01563.
SMRiP01563.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109663. 8 interactors.
DIPiDIP-3784N.
DIP-481N.
IntActiP01563. 1 interactor.
STRINGi9606.ENSP00000369554.

Protein family/group databases

Allergomei9876. Hom s IFN alpha.

PTM databases

iPTMnetiP01563.
PhosphoSitePlusiP01563.
UniCarbKBiP01563.

Polymorphism and mutation databases

BioMutaiIFNA2.
DMDMi124449.

Proteomic databases

EPDiP01563.
PaxDbiP01563.
PeptideAtlasiP01563.
PRIDEiP01563.

Protocols and materials databases

DNASUi3440.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000380206; ENSP00000369554; ENSG00000188379.
GeneIDi3440.
KEGGihsa:3440.
UCSCiuc003zpb.4. human.

Organism-specific databases

CTDi3440.
DisGeNETi3440.
GeneCardsiIFNA2.
H-InvDBHIX0034810.
HGNCiHGNC:5423. IFNA2.
HPAiHPA047557.
MIMi147562. gene.
neXtProtiNX_P01563.
PharmGKBiPA29662.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410J735. Eukaryota.
ENOG410ZH91. LUCA.
HOGENOMiHOG000230500.
HOVERGENiHBG052086.
InParanoidiP01563.
KOiK05414.
OrthoDBiEOG091G0MJ6.
PhylomeDBiP01563.
TreeFamiTF336177.

Enzyme and pathway databases

BioCyciZFISH:G66-31254-MONOMER.
ReactomeiR-HSA-909733. Interferon alpha/beta signaling.
R-HSA-912694. Regulation of IFNA signaling.
R-HSA-933541. TRAF6 mediated IRF7 activation.
R-HSA-983231. Factors involved in megakaryocyte development and platelet production.
SIGNORiP01563.

Miscellaneous databases

EvolutionaryTraceiP01563.
GeneWikiiIFNA2.
GenomeRNAii3440.
PROiP01563.
SOURCEiSearch...

Gene expression databases

CleanExiHS_IFNA2.
GenevisibleiP01563. HS.

Family and domain databases

CDDicd00095. IFab. 1 hit.
Gene3Di1.20.1250.10. 1 hit.
InterProiIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR000471. Interferon_alpha/beta/delta.
[Graphical view]
PANTHERiPTHR11691. PTHR11691. 1 hit.
PfamiPF00143. Interferon. 1 hit.
[Graphical view]
PRINTSiPR00266. INTERFERONAB.
SMARTiSM00076. IFabd. 1 hit.
[Graphical view]
SUPFAMiSSF47266. SSF47266. 1 hit.
PROSITEiPS00252. INTERFERON_A_B_D. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIFNA2_HUMAN
AccessioniPrimary (citable) accession number: P01563
Secondary accession number(s): H2DF54
, H2DF55, P01564, Q14606, Q6DJX8, Q96KI6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 30, 2016
This is version 169 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Pharmaceutical, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.