Reviewed,
UniProtKB/Swiss-Prot P01563 (IFNA2_HUMAN)
Last modified
July 22, 2008.
Version 92.
History...
Clusters with 100%,
90%,
50% identity |
Documents (7) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Interferon alpha-2 Alternative name(s): Interferon alpha-A Short name=LeIF A | ||
| Gene names |
| ||
| Organism | Homo sapiens (Human) | ||
| Taxonomic identifier | 9606 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 188 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. |
| Subcellular location | |
| Polymorphism | Three forms exist; alpha-2a (shown here), alpha-2b and alpha-2c. |
| Pharmaceutical use | Available under the names Roferon-A (Roche) or Intron-A (Schering-Plough). Used as an anticancer drug for its antiproliferative activity. |
| Sequence similarities | Belongs to the alpha/beta interferon family. |
Ontologies
Keywords | |
|---|---|
| Biological process | Antiviral defense |
| Cellular component | Secreted |
| Coding sequence diversity | Polymorphism |
| Domain | Signal |
| Molecular function | Cytokine |
| PTM | Glycoprotein |
| Technical term | 3D-structure Direct protein sequencing Pharmaceutical |
Gene Ontology (GO) | |
| Biological process | cell surface receptor linked signal transduction Ref.6 Traceable author statement. Source: ProtInc cell-cell signalingTraceable author statement. Source: ProtInc induction of apoptosisTraceable author statement. Source: ProtInc inflammatory responseTraceable author statement. Source: ProtInc |
| Molecular function | interferon-alpha/beta receptor binding Ref.6 Traceable author statement. Source: ProtInc |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | |||||||||||||||||||||||||
Molecule processing | |||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 23 | 23 | |||||||||||||||||||||||||||
| Chain | 24 – 188 | 165 | Interferon alpha-2 | ||||||||||||||||||||||||||
Amino acid modifications | |||||||||||||||||||||||||||||
| Glycosylation | 129 | 1 | O-linked (GalNAc...) | ||||||||||||||||||||||||||
| Disulfide bond | 24 ↔ 121 | ||||||||||||||||||||||||||||
| Disulfide bond | 52 ↔ 161 | ||||||||||||||||||||||||||||
Natural variations | |||||||||||||||||||||||||||||
| Natural variant | 46 | 1 | K → R in alpha-2B and alpha-2C. | ||||||||||||||||||||||||||
| Natural variant | 57 | 1 | H → R in alpha-2C. | ||||||||||||||||||||||||||
| Natural variant | 177 | 1 | S → L in a breast cancer sample; somatic mutation. | ||||||||||||||||||||||||||
Secondary structure | |||||||||||||||||||||||||||||
Helix Strand Turn | |||||||||||||||||||||||||||||
| Helix | 33 – 44 | 12 | |||||||||||||||||||||||||||
| Turn | 49 – 54 | 6 | |||||||||||||||||||||||||||
| Helix | 63 – 66 | 4 | |||||||||||||||||||||||||||
| Helix | 76 – 91 | 16 | |||||||||||||||||||||||||||
| Helix | 93 – 98 | 6 | |||||||||||||||||||||||||||
| Helix | 101 – 123 | 23 | |||||||||||||||||||||||||||
| Turn | 126 – 127 | 2 | |||||||||||||||||||||||||||
| Turn | 133 | 1 | |||||||||||||||||||||||||||
| Helix | 134 – 155 | 22 | |||||||||||||||||||||||||||
| Turn | 156 – 157 | 2 | |||||||||||||||||||||||||||
| Helix | 160 – 178 | 19 | |||||||||||||||||||||||||||
| Turn | 179 – 182 | 4 | |||||||||||||||||||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Human leukocyte interferon produced by E. coli is biologically active." Goeddel D.V., Yelverton E., Ullrich A., Heyneker H.L., Miozzari G., Holmes W., Seeburg P.H., Dull T.J., May L., Stebbing N., Crea R., Maeda S., McCandliss R., Sloma A., Tabor J.M., Gross M., Familletti P.C., Pestka S. Nature 287:411-416(1980) [PubMed: 6159538] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. |
| [2] | "The structure of eight distinct cloned human leukocyte interferon cDNAs." Goeddel D.V., Leung D.W., Dull T.J., Gross M., Lawn R.M., McCandliss R., Seeburg P.H., Ullrich A., Yelverton E., Gray P.W. Nature 290:20-26(1981) [PubMed: 6163083] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. |
| [3] | "DNA sequence of a major human leukocyte interferon gene." Lawn R.M., Gross M., Houck C.M., Franke A.E., Gray P.V., Goeddel D.V. Proc. Natl. Acad. Sci. U.S.A. 78:5435-5439(1981) [PubMed: 6170983] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. |
| [4] | "Cloning of human leukocyte interferon cDNA and a strategy for its production in E. coli." Oliver G., Balbas P., Valle F., Soberon X., Bolivar F. Rev. Latinoam. Microbiol. 27:141-150(1985) [PubMed: 3906813] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Tissue: Bone marrow tumor. |
| [5] | "A defective retroviral vector encoding human interferon alpha 2 can transduce human leukemic cell lines." Austruy E., Bagnis C., Carbuccia N., Maroc C., Birg F., Dubreuil P., Mannoni P., Chabannon C. Cancer Gene Ther. 5:247-256(1998) [PubMed: 9694076] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Placenta. |
| [6] | "At least three human type alpha interferons: structure of alpha 2." Streuli M., Nagata S., Weissmann C. Science 209:1343-1347(1980) [PubMed: 6158094] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 7-188. |
| [7] | "Formation of genes coding for hybrid proteins by recombination between related, cloned genes in E. coli." Weber H., Weissmann C. Nucleic Acids Res. 11:5661-5669(1983) [PubMed: 6310510] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 24-188. |
| [8] | "A family of structural genes for human lymphoblastoid (leukocyte-type) interferon." Allen G., Fantes K.H. Nature 287:408-411(1980) [PubMed: 6159537] [Abstract] Cited for: PROTEIN SEQUENCE OF 24-112 AND 136-188. |
| [9] | "Identification of nine interferon-alpha subtypes produced by Sendai virus-induced human peripheral blood leucocytes." Nyman T.A., Toeloe H., Parkkinen J., Kalkkinen N. Biochem. J. 329:295-302(1998) [PubMed: 9425112] [Abstract] Cited for: PROTEIN SEQUENCE OF 24-58. |
| [10] | "Assignment of the disulphide bonds of leukocyte interferon." Wetzel R. Nature 289:606-607(1981) [PubMed: 6162107] [Abstract] Cited for: DISULFIDE BONDS. |
| [11] | "Natural human interferon-alpha 2 is O-glycosylated." Adolf G.R., Kalsner I., Ahorn H., Maurer-Fogy I., Cantell K. Biochem. J. 276:511-518(1991) [PubMed: 2049076] [Abstract] Cited for: GLYCOSYLATION AT THR-129, VARIANTS ALPHA-2B AND ALPHA-2C. |
| [12] | "Interferon-alpha 2 variants in the human genome." Lee N., Ni D., Brissette R., Chou M., Hussain M., Gill D.S., Liao M.-J., Testa D. J. Interferon Cytokine Res. 15:341-349(1995) [PubMed: 7627809] [Abstract] Cited for: POLYMORPHISM. |
| [13] | "A homology model of human interferon alpha-2." Murgolo N.J., Windsor W.T., Hruza A., Reichert P., Tsarbopoulos A., Baldwin S., Huang E., Pramanik B., Ealick S., Trotta P.P. Proteins 17:62-74(1993) [PubMed: 8234245] [Abstract] Cited for: 3D-STRUCTURE MODELING. |
| [14] | "Zinc mediated dimer of human interferon-alpha 2b revealed by X-ray crystallography." Radhakrishnan R., Walter L.J., Hruza A., Reichert P., Trotta P.P., Nagabhushan T.L., Walter M.R. Structure 4:1453-1463(1996) [PubMed: 8994971] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS). |
| [15] | "The three-dimensional high resolution structure of human interferon alpha-2a determined by heteronuclear NMR spectroscopy in solution." Klaus W., Gsell B., Labhardt A.M., Wipf B., Senn H. J. Mol. Biol. 274:661-675(1997) [PubMed: 9417943] [Abstract] Cited for: STRUCTURE BY NMR. |
| [16] | "The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. Velculescu V.E.Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] LEU-177. |
Cross-references
Sequence databases | |||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| J00207 Genomic DNA. Translation: AAB59402.1. V00544 mRNA. Translation: CAA23805.1. V00548 mRNA. Translation: CAA23809.1. V00549 mRNA. Translation: CAA23810.1. Y11834 Genomic DNA. Translation: CAA72532.1. M54886 mRNA. Translation: AAA59181.1. M29883 Genomic DNA. Translation: AAA52715.1. A04970 Unassigned DNA. Translation: CAA00410.1. | |||||||||||||||||||||||||||||||
| PIR | IVHUA2. A93234. I78570. | ||||||||||||||||||||||||||||||
| UniGene | Hs.211575 | ||||||||||||||||||||||||||||||
3D structure databases | |||||||||||||||||||||||||||||||
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| ModBase | Search... | ||||||||||||||||||||||||||||||
Protein-protein interaction databases | |||||||||||||||||||||||||||||||
| DIP | DIP:3784N. DIP:481N. | ||||||||||||||||||||||||||||||
PTM databases | |||||||||||||||||||||||||||||||
| GlycoSuiteDB | P01563. | ||||||||||||||||||||||||||||||
Genome annotation databases | |||||||||||||||||||||||||||||||
| Ensembl | ENSG00000188379. Homo sapiens. [Contig view] | ||||||||||||||||||||||||||||||
Organism-specific databases | |||||||||||||||||||||||||||||||
| H-InvDB | HIX0034810. | ||||||||||||||||||||||||||||||
| HGNC | HGNC:5423. IFNA2. | ||||||||||||||||||||||||||||||
| MIM | 147562. gene. | ||||||||||||||||||||||||||||||
| PharmGKB | PA29662. | ||||||||||||||||||||||||||||||
| GenAtlas | Search... | ||||||||||||||||||||||||||||||
| GeneCards | Search... | ||||||||||||||||||||||||||||||
Phylogenomic databases | |||||||||||||||||||||||||||||||
| HOGENOM | P01563. | ||||||||||||||||||||||||||||||
| HOVERGEN | P01563. | ||||||||||||||||||||||||||||||
Gene expression databases | |||||||||||||||||||||||||||||||
| ArrayExpress | P01563. | ||||||||||||||||||||||||||||||
| CleanEx | HS_IFNA2. | ||||||||||||||||||||||||||||||
| GermOnline | ENSG00000188379. Homo sapiens. | ||||||||||||||||||||||||||||||
Family and domain databases | |||||||||||||||||||||||||||||||
| InterPro | IPR012351. 4_helix_cytokine_core. IPR015589. IFNa. IPR000471. Interferon_abd. [Graphical view] | ||||||||||||||||||||||||||||||
| Gene3D | G3DSA:1.20.1250.10. 4_helix_cytokine_core. 1 hit. | ||||||||||||||||||||||||||||||
| PANTHER | PTHR11691:SF9. IFNa. 1 hit. PTHR11691. Interferon_abd. 1 hit. | ||||||||||||||||||||||||||||||
| Pfam | PF00143. Interferon. 1 hit. [Graphical view] | ||||||||||||||||||||||||||||||
| PRINTS | PR00266. INTERFERONAB. | ||||||||||||||||||||||||||||||
| ProDom | PD000550. Interferon_abd. 1 hit. [Graphical view] [Entries sharing at least one domain] | ||||||||||||||||||||||||||||||
| SMART | SM00076. IFabd. 1 hit. [Graphical view] | ||||||||||||||||||||||||||||||
| PROSITE | PS00252. INTERFERON_A_B_D. 1 hit. [Graphical view] | ||||||||||||||||||||||||||||||
| BLOCKS | Search... | ||||||||||||||||||||||||||||||
Other Resources | |||||||||||||||||||||||||||||||
| DrugBank | DB00034. Interferon Alfa-2a, Recombinant. DB00105. Interferon Alfa-2b, Recombinant. DB00011. Interferon alfa-n1. DB00008. Peginterferon alfa-2a. DB00022. Peginterferon alfa-2b. | ||||||||||||||||||||||||||||||
| LinkHub | P01563. | ||||||||||||||||||||||||||||||
| SOURCE | Search... | ||||||||||||||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||||||||||||||
Entry information
| Entry name | IFNA2_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P01563 Secondary accession number(s): P01564, Q14606, Q96KI6 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 9 Human chromosome 9: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| UniProtKB secondary accession numbers Index of UniProtKB secondary accession numbers |
| SIMILARITY comments Index of protein domains and families |

Clusters with


