Interferon alpha-2 precursor - Homo sapiens (Human)

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Reviewed, UniProtKB/Swiss-Prot P01563 (IFNA2_HUMAN)

Last modified July 7, 2009. Version 101. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Interferon alpha-2
Alternative name(s):
    Interferon alpha-A
      Short name=LeIF A

Gene names

Name:

IFNA2

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	188 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
Subcellular location	Secreted.
Polymorphism	Three forms exist; alpha-2a (shown here), alpha-2b and alpha-2c.
Pharmaceutical use	Available under the names Roferon-A (Roche) or Intron-A (Schering-Plough). Used as an anticancer drug for its antiproliferative activity.
Sequence similarities	Belongs to the alpha/beta interferon family.

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Ontologies

Keywords
Biological process	Antiviral defense
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Cytokine
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Pharmaceutical
Gene Ontology (GO)
Biological process	cell surface receptor linked signal transduction Ref.6 Traceable author statement. Source: ProtInc cell-cell signaling Traceable author statement. Source: ProtInc induction of apoptosis Traceable author statement. Source: ProtInc inflammatory response Traceable author statement. Source: ProtInc response to virus Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cytokine activity Inferred from electronic annotation. Source: UniProtKB-KW interferon-alpha/beta receptor binding Ref.6 Traceable author statement. Source: ProtInc
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 23

Ref.8 Ref.9

Chain

24 – 188

165

Interferon alpha-2

PRO_0000016360

Amino acid modifications

Glycosylation

129

O-linked (GalNAc...) Ref.11

Disulfide bond

Disulfide bond

Natural variations

Natural variant

A → D: dbSNP rs35971916.

VAR_055972

Natural variant

K → R in alpha-2B and alpha-2C.

VAR_004012

Natural variant

H → R in alpha-2C.

VAR_013001

Natural variant

177

S → L in a breast cancer sample; somatic mutation. Ref.16

VAR_036329

Secondary structure

188

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P01563-1 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 101DD21D394CBF97
Show »

FASTA

188

21,550

        10         20         30         40         50         60 
MALTFALLVA LLVLSCKSSC SVGCDLPQTH SLGSRRTLML LAQMRKISLF SCLKDRHDFG 

        70         80         90        100        110        120 
FPQEEFGNQF QKAETIPVLH EMIQQIFNLF STKDSSAAWD ETLLDKFYTE LYQQLNDLEA 

       130        140        150        160        170        180 
CVIQGVGVTE TPLMKEDSIL AVRKYFQRIT LYLKEKKYSP CAWEVVRAEI MRSFSLSTNL 


QESLRSKE

« Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human leukocyte interferon produced by E. coli is biologically active." Goeddel D.V., Yelverton E., Ullrich A., Heyneker H.L., Miozzari G., Holmes W., Seeburg P.H., Dull T.J., May L., Stebbing N., Crea R., Maeda S., McCandliss R., Sloma A., Tabor J.M., Gross M., Familletti P.C., Pestka S. Nature 287:411-416(1980) [PubMed: 6159538] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]	"The structure of eight distinct cloned human leukocyte interferon cDNAs." Goeddel D.V., Leung D.W., Dull T.J., Gross M., Lawn R.M., McCandliss R., Seeburg P.H., Ullrich A., Yelverton E., Gray P.W. Nature 290:20-26(1981) [PubMed: 6163083] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[3]	"DNA sequence of a major human leukocyte interferon gene." Lawn R.M., Gross M., Houck C.M., Franke A.E., Gray P.V., Goeddel D.V. Proc. Natl. Acad. Sci. U.S.A. 78:5435-5439(1981) [PubMed: 6170983] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[4]	"Cloning of human leukocyte interferon cDNA and a strategy for its production in E. coli." Oliver G., Balbas P., Valle F., Soberon X., Bolivar F. Rev. Latinoam. Microbiol. 27:141-150(1985) [PubMed: 3906813] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Bone marrow tumor.
[5]	"A defective retroviral vector encoding human interferon alpha 2 can transduce human leukemic cell lines." Austruy E., Bagnis C., Carbuccia N., Maroc C., Birg F., Dubreuil P., Mannoni P., Chabannon C. Cancer Gene Ther. 5:247-256(1998) [PubMed: 9694076] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Placenta.
[6]	"At least three human type alpha interferons: structure of alpha 2." Streuli M., Nagata S., Weissmann C. Science 209:1343-1347(1980) [PubMed: 6158094] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 7-188.
[7]	"Formation of genes coding for hybrid proteins by recombination between related, cloned genes in E. coli." Weber H., Weissmann C. Nucleic Acids Res. 11:5661-5669(1983) [PubMed: 6310510] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 24-188.
[8]	"A family of structural genes for human lymphoblastoid (leukocyte-type) interferon." Allen G., Fantes K.H. Nature 287:408-411(1980) [PubMed: 6159537] [Abstract] Cited for: PROTEIN SEQUENCE OF 24-112 AND 136-188.
[9]	"Identification of nine interferon-alpha subtypes produced by Sendai virus-induced human peripheral blood leucocytes." Nyman T.A., Toeloe H., Parkkinen J., Kalkkinen N. Biochem. J. 329:295-302(1998) [PubMed: 9425112] [Abstract] Cited for: PROTEIN SEQUENCE OF 24-58.
[10]	"Assignment of the disulphide bonds of leukocyte interferon." Wetzel R. Nature 289:606-607(1981) [PubMed: 6162107] [Abstract] Cited for: DISULFIDE BONDS.
[11]	"Natural human interferon-alpha 2 is O-glycosylated." Adolf G.R., Kalsner I., Ahorn H., Maurer-Fogy I., Cantell K. Biochem. J. 276:511-518(1991) [PubMed: 2049076] [Abstract] Cited for: GLYCOSYLATION AT THR-129, VARIANTS ALPHA-2B AND ALPHA-2C.
[12]	"Interferon-alpha 2 variants in the human genome." Lee N., Ni D., Brissette R., Chou M., Hussain M., Gill D.S., Liao M.-J., Testa D. J. Interferon Cytokine Res. 15:341-349(1995) [PubMed: 7627809] [Abstract] Cited for: POLYMORPHISM.
[13]	"A homology model of human interferon alpha-2." Murgolo N.J., Windsor W.T., Hruza A., Reichert P., Tsarbopoulos A., Baldwin S., Huang E., Pramanik B., Ealick S., Trotta P.P. Proteins 17:62-74(1993) [PubMed: 8234245] [Abstract] Cited for: 3D-STRUCTURE MODELING.
[14]	"Zinc mediated dimer of human interferon-alpha 2b revealed by X-ray crystallography." Radhakrishnan R., Walter L.J., Hruza A., Reichert P., Trotta P.P., Nagabhushan T.L., Walter M.R. Structure 4:1453-1463(1996) [PubMed: 8994971] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS).
[15]	"The three-dimensional high resolution structure of human interferon alpha-2a determined by heteronuclear NMR spectroscopy in solution." Klaus W., Gsell B., Labhardt A.M., Wipf B., Senn H. J. Mol. Biol. 274:661-675(1997) [PubMed: 9417943] [Abstract] Cited for: STRUCTURE BY NMR.
[16]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. Velculescu V.E. Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] LEU-177.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

J00207 Genomic DNA. Translation: AAB59402.1.
V00544 mRNA. Translation: CAA23805.1.
V00548 mRNA. Translation: CAA23809.1.
V00549 mRNA. Translation: CAA23810.1.
Y11834 Genomic DNA. Translation: CAA72532.1.
M54886 mRNA. Translation: AAA59181.1.
M29883 Genomic DNA. Translation: AAA52715.1.
A04970 Unassigned DNA. Translation: CAA00410.1.

IPI

IPI00307184.

PIR

IVHUA2. A93234.
I78570.

UniGene

Hs.211575

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ITF	NMR	-	A	24-188	[»]
1RH2	X-ray	2.90	A/B/C/D/E/F	24-188	[»]
2HIE	model	-	A	24-188	[»]
2HYM	NMR	-	B	24-188	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:3784N.
DIP:481N.

PTM databases

GlycoSuiteDB

P01563.

Proteomic databases

PRIDE

P01563.

Genome annotation databases

Ensembl

ENSG00000188379. Homo sapiens. [Contig view]

Organism-specific databases

GeneCards

GC09M021374.

H-InvDB

HIX0034810.

HGNC

HGNC:5423. IFNA2.

MIM

147562. gene.

PharmGKB

PA29662.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

P01563.

HOVERGEN

P01563.

Enzyme and pathway databases

Pathway_Interaction_DB

cd8tcrdownstreampathway. Downstream signaling in naive CD8+ T cells.

Gene expression databases

ArrayExpress

P01563.

Bgee

P01563.

CleanEx

HS_IFNA2.

GermOnline

ENSG00000188379. Homo sapiens.

Family and domain databases

InterPro

IPR012351. 4_helix_cytokine_core.
IPR000471. IInterferon_alpha/beta/delta.
IPR015589. Interferon_alpha.
[Graphical view]

Gene3D

G3DSA:1.20.1250.10. 4_helix_cytokine_core. 1 hit.

PANTHER

PTHR11691:SF9. IFNa. 1 hit.
PTHR11691. Interferon_abd. 1 hit.

Pfam

PF00143. Interferon. 1 hit.
[Graphical view]

PRINTS

PR00266. INTERFERONAB.

ProDom

PD000550. Interferon_abd. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00076. IFabd. 1 hit.
[Graphical view]

PROSITE

PS00252. INTERFERON_A_B_D. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

DrugBank

DB00034. Interferon Alfa-2a, Recombinant.
DB00105. Interferon Alfa-2b, Recombinant.
DB00011. Interferon alfa-n1.
DB00008. Peginterferon alfa-2a.
DB00022. Peginterferon alfa-2b.

SOURCE

Search...

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Entry information

Entry name

IFNA2_HUMAN

Accession

Primary (citable) accession number: P01563
Secondary accession number(s): P01564, Q14606, Q96KI6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	July 7, 2009
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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