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Reviewed, UniProtKB/Swiss-Prot P01563 (IFNA2_HUMAN)

Last modified July 7, 2009. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Interferon alpha-2
Alternative name(s):
    Interferon alpha-A
      Short name=LeIF A
Gene names
Name: IFNA2
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length188 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.

Subcellular location

Secreted.

Polymorphism

Three forms exist; alpha-2a (shown here), alpha-2b and alpha-2c.

Pharmaceutical use

Available under the names Roferon-A (Roche) or Intron-A (Schering-Plough). Used as an anticancer drug for its antiproliferative activity.

Sequence similarities

Belongs to the alpha/beta interferon family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Ref.8 Ref.9
Chain24 – 188165Interferon alpha-2
PRO_0000016360

Amino acid modifications

Glycosylation1291O-linked (GalNAc...) Ref.11
CAR_000049
Disulfide bond24 ↔ 121 Ref.10
Disulfide bond52 ↔ 161 Ref.10

Natural variations

Natural variant61A → D: dbSNP rs35971916.
VAR_055972
Natural variant461K → R in alpha-2B and alpha-2C.
VAR_004012
Natural variant571H → R in alpha-2C.
VAR_013001
Natural variant1771S → L in a breast cancer sample; somatic mutation. Ref.16
VAR_036329

Secondary structure

.................... 188
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P01563-1 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 101DD21D394CBF97

FASTA18821,550
        10         20         30         40         50         60 
MALTFALLVA LLVLSCKSSC SVGCDLPQTH SLGSRRTLML LAQMRKISLF SCLKDRHDFG 

        70         80         90        100        110        120 
FPQEEFGNQF QKAETIPVLH EMIQQIFNLF STKDSSAAWD ETLLDKFYTE LYQQLNDLEA 

       130        140        150        160        170        180 
CVIQGVGVTE TPLMKEDSIL AVRKYFQRIT LYLKEKKYSP CAWEVVRAEI MRSFSLSTNL 


QESLRSKE 

« Hide

References

« Hide 'large scale' references
[1]"Human leukocyte interferon produced by E. coli is biologically active."
Goeddel D.V., Yelverton E., Ullrich A., Heyneker H.L., Miozzari G., Holmes W., Seeburg P.H., Dull T.J., May L., Stebbing N., Crea R., Maeda S., McCandliss R., Sloma A., Tabor J.M., Gross M., Familletti P.C., Pestka S.
Nature 287:411-416(1980) [PubMed: 6159538] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]"The structure of eight distinct cloned human leukocyte interferon cDNAs."
Goeddel D.V., Leung D.W., Dull T.J., Gross M., Lawn R.M., McCandliss R., Seeburg P.H., Ullrich A., Yelverton E., Gray P.W.
Nature 290:20-26(1981) [PubMed: 6163083] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[3]"DNA sequence of a major human leukocyte interferon gene."
Lawn R.M., Gross M., Houck C.M., Franke A.E., Gray P.V., Goeddel D.V.
Proc. Natl. Acad. Sci. U.S.A. 78:5435-5439(1981) [PubMed: 6170983] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[4]"Cloning of human leukocyte interferon cDNA and a strategy for its production in E. coli."
Oliver G., Balbas P., Valle F., Soberon X., Bolivar F.
Rev. Latinoam. Microbiol. 27:141-150(1985) [PubMed: 3906813] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Bone marrow tumor.
[5]"A defective retroviral vector encoding human interferon alpha 2 can transduce human leukemic cell lines."
Austruy E., Bagnis C., Carbuccia N., Maroc C., Birg F., Dubreuil P., Mannoni P., Chabannon C.
Cancer Gene Ther. 5:247-256(1998) [PubMed: 9694076] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Placenta.
[6]"At least three human type alpha interferons: structure of alpha 2."
Streuli M., Nagata S., Weissmann C.
Science 209:1343-1347(1980) [PubMed: 6158094] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 7-188.
[7]"Formation of genes coding for hybrid proteins by recombination between related, cloned genes in E. coli."
Weber H., Weissmann C.
Nucleic Acids Res. 11:5661-5669(1983) [PubMed: 6310510] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 24-188.
[8]"A family of structural genes for human lymphoblastoid (leukocyte-type) interferon."
Allen G., Fantes K.H.
Nature 287:408-411(1980) [PubMed: 6159537] [Abstract]
Cited for: PROTEIN SEQUENCE OF 24-112 AND 136-188.
[9]"Identification of nine interferon-alpha subtypes produced by Sendai virus-induced human peripheral blood leucocytes."
Nyman T.A., Toeloe H., Parkkinen J., Kalkkinen N.
Biochem. J. 329:295-302(1998) [PubMed: 9425112] [Abstract]
Cited for: PROTEIN SEQUENCE OF 24-58.
[10]"Assignment of the disulphide bonds of leukocyte interferon."
Wetzel R.
Nature 289:606-607(1981) [PubMed: 6162107] [Abstract]
Cited for: DISULFIDE BONDS.
[11]"Natural human interferon-alpha 2 is O-glycosylated."
Adolf G.R., Kalsner I., Ahorn H., Maurer-Fogy I., Cantell K.
Biochem. J. 276:511-518(1991) [PubMed: 2049076] [Abstract]
Cited for: GLYCOSYLATION AT THR-129, VARIANTS ALPHA-2B AND ALPHA-2C.
[12]"Interferon-alpha 2 variants in the human genome."
Lee N., Ni D., Brissette R., Chou M., Hussain M., Gill D.S., Liao M.-J., Testa D.
J. Interferon Cytokine Res. 15:341-349(1995) [PubMed: 7627809] [Abstract]
Cited for: POLYMORPHISM.
[13]"A homology model of human interferon alpha-2."
Murgolo N.J., Windsor W.T., Hruza A., Reichert P., Tsarbopoulos A., Baldwin S., Huang E., Pramanik B., Ealick S., Trotta P.P.
Proteins 17:62-74(1993) [PubMed: 8234245] [Abstract]
Cited for: 3D-STRUCTURE MODELING.
[14]"Zinc mediated dimer of human interferon-alpha 2b revealed by X-ray crystallography."
Radhakrishnan R., Walter L.J., Hruza A., Reichert P., Trotta P.P., Nagabhushan T.L., Walter M.R.
Structure 4:1453-1463(1996) [PubMed: 8994971] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS).
[15]"The three-dimensional high resolution structure of human interferon alpha-2a determined by heteronuclear NMR spectroscopy in solution."
Klaus W., Gsell B., Labhardt A.M., Wipf B., Senn H.
J. Mol. Biol. 274:661-675(1997) [PubMed: 9417943] [Abstract]
Cited for: STRUCTURE BY NMR.
[16]"The consensus coding sequences of human breast and colorectal cancers."
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. expand/collapse author list , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.
Science 314:268-274(2006) [PubMed: 16959974] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] LEU-177.
+Additional computationally mapped references.

Cross-references

Sequence databases

J00207 Genomic DNA. Translation: AAB59402.1.
V00544 mRNA. Translation: CAA23805.1.
V00548 mRNA. Translation: CAA23809.1.
V00549 mRNA. Translation: CAA23810.1.
Y11834 Genomic DNA. Translation: CAA72532.1.
M54886 mRNA. Translation: AAA59181.1.
M29883 Genomic DNA. Translation: AAA52715.1.
A04970 Unassigned DNA. Translation: CAA00410.1.
IPIIPI00307184.
PIRIVHUA2. A93234.
I78570.
UniGeneHs.211575

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1ITFNMR-A24-188[»]
1RH2X-ray2.90A/B/C/D/E/F24-188[»]
2HIEmodel-A24-188[»]
2HYMNMR-B24-188[»]
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:3784N.
DIP:481N.

PTM databases

GlycoSuiteDBP01563.

Proteomic databases

PRIDEP01563.

Genome annotation databases

EnsemblENSG00000188379. Homo sapiens. [Contig view]

Organism-specific databases

GeneCardsGC09M021374.
H-InvDBHIX0034810.
HGNCHGNC:5423. IFNA2.
MIM147562. gene.
PharmGKBPA29662.
GenAtlasSearch...

Phylogenomic databases

HOGENOMP01563.
HOVERGENP01563.

Enzyme and pathway databases

Pathway_Interaction_DBcd8tcrdownstreampathway. Downstream signaling in naive CD8+ T cells.

Gene expression databases

ArrayExpressP01563.
BgeeP01563.
CleanExHS_IFNA2.
GermOnlineENSG00000188379. Homo sapiens.

Family and domain databases

InterProIPR012351. 4_helix_cytokine_core.
IPR000471. IInterferon_alpha/beta/delta.
IPR015589. Interferon_alpha.
[Graphical view]
Gene3DG3DSA:1.20.1250.10. 4_helix_cytokine_core. 1 hit.
PANTHERPTHR11691:SF9. IFNa. 1 hit.
PTHR11691. Interferon_abd. 1 hit.
PfamPF00143. Interferon. 1 hit.
[Graphical view]
PRINTSPR00266. INTERFERONAB.
ProDomPD000550. Interferon_abd. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00076. IFabd. 1 hit.
[Graphical view]
PROSITEPS00252. INTERFERON_A_B_D. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

DrugBankDB00034. Interferon Alfa-2a, Recombinant.
DB00105. Interferon Alfa-2b, Recombinant.
DB00011. Interferon alfa-n1.
DB00008. Peginterferon alfa-2a.
DB00022. Peginterferon alfa-2b.
SOURCESearch...

Entry information

Entry nameIFNA2_HUMAN
AccessionPrimary (citable) accession number: P01563
Secondary accession number(s): P01564, Q14606, Q96KI6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: July 7, 2009
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Human chromosome 9

Human chromosome 9: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents