P01562 (IFNA1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Interferon alpha-1/13
Short name=IFN-alpha-1/13

Alternative name(s):
Interferon alpha-D
Short name=LeIF D

Gene names

Name:	IFNA1
AND
Name:	IFNA13

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	189 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. Ref.15
Subcellular location	Secreted.
Polymorphism	Two forms exist; alpha-1a (shown here) and alpha-1b.
Miscellaneous	Interferons alpha-1 and alpha-13 have identical protein sequences.
Sequence similarities	Belongs to the alpha/beta interferon family.

Ontologies

Keywords
Biological process	Antiviral defense
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Cytokine
PTM	Disulfide bond
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	blood coagulation Traceable author statement. Source: Reactome defense response to virus Inferred from electronic annotation. Source: UniProtKB-KW regulation of type I interferon-mediated signaling pathway Traceable author statement. Source: Reactome type I interferon-mediated signaling pathway Traceable author statement. Source: Reactome
Cellular_component	extracellular region Traceable author statement. Source: Reactome extracellular space Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	interferon-alpha/beta receptor binding Traceable author statement Ref.5. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 23

23

Ref.15 Ref.16

Chain

24 – 189

166

Interferon alpha-1/13

PRO_0000016359

Amino acid modifications

Disulfide bond

24 ↔ 122

By similarity

Disulfide bond

52 ↔ 162

Ref.18

Natural variations

Natural variant

10

1

V → A. Ref.8 Ref.9 Ref.12
Corresponds to variant rs1758567 [ dbSNP | Ensembl ].

VAR_024508

Natural variant

137

1

A → V in alpha-1B. Ref.9
Corresponds to variant rs2230050 [ dbSNP | Ensembl ].

VAR_013000

Natural variant

163

1

A → G. Ref.9
Corresponds to variant rs33965070 [ dbSNP | Ensembl ].

VAR_025173

Experimental info

Sequence conflict

116

1

L → P in AAL35223. Ref.14

Sequence conflict

172

1

M → V in AAL35223. Ref.14

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

189

Helix Strand Turn

Details...

Helix

35 – 43

9

Helix

49 – 51

3

Turn

53 – 55

3

Helix

63 – 66

4

Helix

77 – 91

15

Helix

94 – 99

6

Helix

102 – 122

21

Helix

137 – 156

20

Turn

157 – 159

3

Helix

161 – 178

18

Sequences

	Sequence		Length	Mass (Da)
	P01562 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: F32F9CB969606B69	FASTA	189	21,725
	10 20 30 40 50 60 MASPFALLMV LVVLSCKSSC SLGCDLPETH SLDNRRTLML LAQMSRISPS SCLMDRHDFG 70 80 90 100 110 120 FPQEEFDGNQ FQKAPAISVL HELIQQIFNL FTTKDSSAAW DEDLLDKFCT ELYQQLNDLE 130 140 150 160 170 180 ACVMQEERVG ETPLMNADSI LAVKKYFRRI TLYLTEKKYS PCAWEVVRAE IMRSLSLSTN LQERLRRKE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of a cloned human leukocyte interferon cDNA." Mantei N., Schwarzstein M., Streuli M., Panem S., Nagata S., Weissmann C. Gene 10:1-10(1980) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Human leukocyte and fibroblast interferons are structurally related." Taniguchi T., Mantei N., Schwarzstein M., Nagata S., Muramatsu M., Weissmann C. Nature 285:547-549(1980) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The structure of eight distinct cloned human leukocyte interferon cDNAs." Goeddel D.V., Leung D.W., Dull T.J., Gross M., Lawn R.M., McCandliss R., Seeburg P.H., Ullrich A., Yelverton E., Gray P.W. Nature 290:20-26(1981) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Two non-allelic human interferon alpha genes with identical coding regions." Todokoro K., Kioussis D., Weissmann C. EMBO J. 3:1809-1812(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"Structural relationship of human interferon alpha genes and pseudogenes." Henco K., Brosius J., Fujisawa A., Fujisawa J., Haynes J.R., Hochstadt J., Kovacic T., Pasek M., Schamboeck A., Schmid J., Todokoro K., Waelchli M., Nagata S., Weissmann C. J. Mol. Biol. 185:227-260(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]	"Two distinct families of human and bovine interferon-alpha genes are coordinately expressed and encode functional polypeptides." Capon D.J., Shepard H.M., Goeddel D.V. Mol. Cell. Biol. 5:768-779(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[7]	"Novel cis-active structures in the coding region mediate CRM1-dependent nuclear export of IFN-alpha 1 mRNA." Kimura T., Hashimoto I., Nishizawa M., Ito S., Yamada H. Med. Mol. Morphol. 43:145-157(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[8]	Rostoks N. Submitted (DEC-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ALA-10.
[9]	NIEHS SNPs program Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ALA-10; VAL-137 AND GLY-163.
[10]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[11]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[12]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-10. Tissue: Cerebellum.
[13]	"Formation of genes coding for hybrid proteins by recombination between related, cloned genes in E. coli." Weber H., Weissmann C. Nucleic Acids Res. 11:5661-5669(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 24-189.
[14]	Chen H.H., Yu X.B. Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 24-189. Tissue: Leukocyte.
[15]	"Purification and characterization of multiple components of human lymphoblastoid interferon-alpha." Zoon K.C., Miller D., Bekisz J., zur Nedden D., Enterline J.C., Nguyen N.Y., Hu R.-Q. J. Biol. Chem. 267:15210-15216(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 24-59, FUNCTION.
[16]	"Identification of nine interferon-alpha subtypes produced by Sendai virus-induced human peripheral blood leucocytes." Nyman T.A., Toeloe H., Parkkinen J., Kalkkinen N. Biochem. J. 329:295-302(1998) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 24-58.
[17]	"IFN-alpha-1a gene is the major variant in the North American population." Hussain M., Ni D., Gill D., Liao M.-J. J. Interferon Cytokine Res. 20:763-768(2000) [PubMed] [Europe PMC] [Abstract] Cited for: POLYMORPHISM.
[18]	"Structural insights into a human anti-IFN antibody exerting therapeutic potential for systemic lupus erythematosus." Ouyang S., Gong B., Li J.Z., Zhao L.X., Wu W., Zhang F.S., Sun L., Wang S.J., Pan M., Li C., Liang W., Shaw N., Zheng J., Zhao G.P., Wang Y., Liu Z.J., Liang M. J. Mol. Med. 90:837-846(2012) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 24-189 IN COMPLEX WITH ANTIBODY, DISULFIDE BOND.
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

V00537 mRNA. Translation: CAA23798.1.
J00210 Genomic DNA. Translation: AAB59403.1.
V00538 mRNA. Translation: CAA23799.1.
X00803 Genomic DNA. Translation: CAA25381.1.
X75934 Genomic DNA. Translation: CAA53538.1.
AB445100 Genomic DNA. Translation: BAI99735.1.
DQ185447 Genomic DNA. Translation: ABA03167.1.
AL353732 Genomic DNA. Translation: CAH72904.1.
AL353732 Genomic DNA. Translation: CAH72911.1.
CH471071 Genomic DNA. Translation: EAW58609.1.
BC069427 mRNA. Translation: AAH69427.1.
BC074928 mRNA. Translation: AAH74928.1.
BC074929 mRNA. Translation: AAH74929.1.
BC093988 mRNA. Translation: AAH93988.1.
BC112002 mRNA. Translation: AAI12003.1.
BC112300 mRNA. Translation: AAI12301.1.
BC112302 mRNA. Translation: AAI12303.1.
M29884 Genomic DNA. Translation: AAA52714.1.
AF439447 Genomic DNA. Translation: AAL35223.1.

IPI

IPI00003072.

PIR

IVHUA1. C23285.
F42753.
H42753.

RefSeq

NP_008831.3. NM_006900.3.
NP_076918.1. NM_024013.2.

UniGene

Hs.37026.
Hs.533471.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3UX9	X-ray	2.80	A/C	24-189	[»]

ProteinModelPortal

P01562.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-6020N.

STRING

9606.ENSP00000276927.

PTM databases

PhosphoSite

P01562.

Polymorphism databases

DMDM

124455.

Proteomic databases

PaxDb

P01562.

PRIDE

P01562.

Protocols and materials databases

DNASU

3439.
3447.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000276927; ENSP00000276927; ENSG00000197919.

GeneID

3439.
3447.

KEGG

hsa:3439.
hsa:3447.

UCSC

uc003zpd.2. human.

Organism-specific databases

CTD

3439.
3447.

GeneCards

GC09M021357.
GC09P021430.

HGNC

HGNC:5417. IFNA1.
HGNC:5419. IFNA13.

HPA

HPA047557.

MIM

147578. gene.
147660. gene.

neXtProt

NX_P01562.

PharmGKB

PA29658.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG40434.

HOVERGEN

HBG052086.

InParanoid

P01562.

KO

K05414.

OMA

ERITIYL.

OrthoDB

EOG4SF973.

PhylomeDB

P01562.

Enzyme and pathway databases

Pathway_Interaction_DB

cd8tcrdownstreampathway. Downstream signaling in naive CD8+ T cells.

Reactome

REACT_604. Hemostasis.
REACT_6900. Immune System.

Gene expression databases

Bgee

P01562.

CleanEx

HS_IFNA1.
HS_IFNA13.

Genevestigator

P01562.

GermOnline

ENSG00000197919. Homo sapiens.

Family and domain databases

Gene3D

1.20.1250.10. 1 hit.

InterPro

IPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR015589. Interferon_alpha.
IPR000471. Interferon_alpha/beta/delta.
[Graphical view]

PANTHER

PTHR11691. PTHR11691. 1 hit.
PTHR11691:SF9. PTHR11691:SF9. 1 hit.

Pfam

PF00143. Interferon. 1 hit.
[Graphical view]

PRINTS

PR00266. INTERFERONAB.

SMART

SM00076. IFabd. 1 hit.
[Graphical view]

SUPFAM

SSF47266. 4_helix_cytokine. 1 hit.

PROSITE

PS00252. INTERFERON_A_B_D. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

13552.

SOURCE

Search...

Entry information

Entry name

IFNA1_HUMAN

Accession

Primary (citable) accession number: P01562
Secondary accession number(s): D4Q9M8 , Q14605, Q2M1L8, Q52LB8, Q5VYQ2, Q7M4Q1, Q8WZ68, Q9UMJ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	May 1, 2013
This is version 140 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.