P01552 (ETXB_STAAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Enterotoxin type B

Alternative name(s):
SEB

Gene names

Name:

entB

Organism

Staphylococcus aureus

Taxonomic identifier

1280 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Staphylococcal enterotoxins cause the intoxication staphylococcal food poisoning syndrome. The illness characterized by high fever, hypotension, diarrhea, shock, and in some cases death.
Subcellular location	Secreted.
Sequence similarities	Belongs to the staphylococcal/streptococcal toxin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Enterotoxin Superantigen Toxin
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 27

27

Ref.3

Chain

28 – 266

239

Enterotoxin type B

PRO_0000035606

Amino acid modifications

Disulfide bond

120 ↔ 140

Experimental info

Sequence conflict

56 – 58

3

DDN → NND AA sequence Ref.3

Sequence conflict

69 – 77

9

DQFLYFDLI → NEFFDLIYL AA sequence Ref.3

Sequence conflict

118

1

Missing AA sequence Ref.3

Sequence conflict

128 – 130

3

DIN → NID AA sequence Ref.3

Sequence conflict

133 – 135

3

QTD → ENT AA sequence Ref.3

Sequence conflict

149 – 150

2

NG → GN AA sequence Ref.3

Sequence conflict

156

1

Y → YY AA sequence Ref.3

Sequence conflict

185 – 186

2

QE → EQ AA sequence Ref.3

Sequence conflict

233

1

D → N AA sequence Ref.3

Sequence conflict

246 – 247

2

DN → ND AA sequence Ref.3

Secondary structure

1

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266

Helix Strand Turn

Details...

Beta strand

33 – 36

4

Helix

41 – 43

3

Helix

49 – 52

4

Helix

53 – 55

3

Beta strand

60 – 66

7

Beta strand

67 – 69

3

Beta strand

75 – 78

4

Turn

83 – 85

3

Beta strand

89 – 94

6

Helix

98 – 104

7

Beta strand

107 – 113

7

Turn

128 – 131

4

Beta strand

138 – 142

5

Beta strand

145 – 148

4

Turn

149 – 151

3

Beta strand

152 – 165

14

Beta strand

168 – 183

16

Helix

184 – 199

16

Beta strand

204 – 206

3

Beta strand

208 – 218

11

Beta strand

221 – 226

6

Beta strand

231 – 233

3

Helix

237 – 241

5

Helix

242 – 246

5

Beta strand

249 – 251

3

Turn

252 – 254

3

Beta strand

255 – 263

9

Sequences

	Sequence		Length	Mass (Da)
	P01552 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: B6D417F61CF018B0	FASTA	266	31,436
	10 20 30 40 50 60 MYKRLFISHV ILIFALILVI STPNVLAESQ PDPKPDELHK SSKFTGLMEN MKVLYDDNHV 70 80 90 100 110 120 SAINVKSIDQ FLYFDLIYSI KDTKLGNYDN VRVEFKNKDL ADKYKDKYVD VFGANYYYQC 130 140 150 160 170 180 YFSKKTNDIN SHQTDKRKTC MYGGVTEHNG NQLDKYRSIT VRVFEDGKNL LSFDVQTNKK 190 200 210 220 230 240 KVTAQELDYL TRHYLVKNKK LYEFNNSPYE TGYIKFIENE NSFWYDMMPA PGDKFDQSKY 250 260 LMMYNDNKMV DSKDVKIEVY LTTKKK

References

[1]	"Nucleotide sequence of the enterotoxin B gene from Staphylococcus aureus." Jones C.L., Khan S.A. J. Bacteriol. 166:29-33(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Molecular cloning of staphylococcal enterotoxin B gene in Escherichia coli and Staphylococcus aureus." Ranelli D.M., Jones C.L., Johns M.B., Mussey G.J., Khan S.A. Proc. Natl. Acad. Sci. U.S.A. 82:5850-5854(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 40-91.
[3]	"The primary structure of staphylococcal enterotoxin B. 3. The cyanogen bromide peptides of reduced and aminoethylated enterotoxin B, and the complete amino acid sequence." Huang I.-Y., Bergdoll M.S. J. Biol. Chem. 245:3518-3525(1970) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 28-266 (S-6).
[4]	"Crystal structure of staphylococcal enterotoxin B, a superantigen." Swaminathan S., Furey W.F. Jr., Pletcher J., Sax M. Nature 359:801-806(1992) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
[5]	"Three-dimensional structure of a human class II histocompatibility molecule complexed with superantigen." Jardetzky T.S., Brown J.H., Gorga J.C., Stern L.J., Urban R.G., Chi Y.I., Stauffacher C., Strominger J.L., Wiley D.C. Nature 368:711-718(1994) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF COMPLEX WITH MHC II.
[6]	"Three-dimensional structure of the complex between a T cell receptor beta chain and the superantigen staphylococcal enterotoxin B." Li H., Llera A., Tsuchiya D., Leder L., Ysern X., Schlievert P.M., Karjalainen K., Mariuzza R.A. Immunity 9:807-816(1998) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF COMPLEX WITH TCR.
[7]	"Crystal structure of microbial superantigen staphylococcal enterotoxin B at 1.5-A resolution: implications for superantigen recognition by MHC class II molecules and T-cell receptors." Papageorgiou A.C., Tranter H.S., Acharya K.R. J. Mol. Biol. 277:61-79(1998) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M11118 Genomic DNA. Translation: AAA88550.1.

PIR

ENSAB6. S27360.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1D5M	X-ray	2.00	C	28-266	[»]
1D5X	X-ray	2.45	C	28-266	[»]
1D5Z	X-ray	2.00	C	28-266	[»]
1D6E	X-ray	2.45	C	28-266	[»]
1GOZ	X-ray	2.00	A/B	28-266	[»]
1SBB	X-ray	2.40	B/D	28-266	[»]
1SE3	X-ray	2.30	A	28-266	[»]
1SE4	X-ray	1.90	A	28-266	[»]
1SEB	X-ray	2.70	D/H	29-262	[»]
2SEB	X-ray	2.50	D	28-266	[»]
3GP7	X-ray	1.90	A/B	28-266	[»]
3R8B	X-ray	2.95	A/C/E/G/I/K/M/O	28-266	[»]
3SEB	X-ray	1.48	A	28-265	[»]

ProteinModelPortal

P01552.

SMR

P01552. Positions 28-266.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-35541N.

IntAct

P01552. 1 interaction.

Protein family/group databases

Allergome

2140. Sta a SEB.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

3.10.20.120. 1 hit.

InterPro

IPR008992. Enterotoxin_bac.
IPR006126. Staph/Strept_toxin_CS.
IPR006173. Staph_tox_OB.
IPR016091. SuperAg_toxin_C.
IPR013307. Superantigen_bac.
IPR006123. Toxin_b-grasp_Staph/Strep.
IPR006177. Toxin_bac.
[Graphical view]

Pfam

PF02876. Stap_Strp_tox_C. 1 hit.
PF01123. Stap_Strp_toxin. 1 hit.
[Graphical view]

PRINTS

PR00279. BACTRLTOXIN.
PR01898. SAGSUPRFAMLY.

SUPFAM

SSF50203. Bact_endotox. 1 hit.
SSF54334. Stap/Strep_toxin. 1 hit.

PROSITE

PS00277. STAPH_STREP_TOXIN_1. 1 hit.
PS00278. STAPH_STREP_TOXIN_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P01552.

Entry information

Entry name

ETXB_STAAU

Accession

Primary (citable) accession number: P01552

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	August 13, 1987
Last modified:	May 1, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program