P01542 (CRAM_CRAAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Crambin

Gene names

Name:

THI2

Organism

Crambe hispanica subsp. abyssinica (Abyssinian kale) (Crambe abyssinica)

Taxonomic identifier

3721 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Brassiceae › Crambe › Crambe hispanica

Protein attributes

Sequence length	46 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The function of this hydrophobic plant seed protein is not known.
Subcellular location	Secreted.
Miscellaneous	Two isoforms exists, a major form PL (shown here) and a minor form SI.
Sequence similarities	Belongs to the plant thionin (TC 1.C.44) family. [View classification]

Ontologies

Keywords
Biological process	Plant defense
Cellular component	Secreted
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	defense response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 46

46

Crambin

PRO_0000221479

Amino acid modifications

Disulfide bond

3 ↔ 40

Ref.2

Disulfide bond

4 ↔ 32

Ref.2

Disulfide bond

16 ↔ 26

Ref.2

Natural variations

Natural variant

22

1

P → S in isoform SI.

Natural variant

25

1

L → I in isoform SI.

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

46

Helix Strand Turn

Details...

Beta strand

2 – 6

5

Helix

7 – 17

11

Turn

18 – 22

5

Helix

26 – 30

5

Beta strand

36 – 38

3

Helix

42 – 44

3

Sequences

	Sequence		Length	Mass (Da)
	P01542 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: 919E68AF159EF722	FASTA	46	4,736
	10 20 30 40 TTCCPSIVAR SNFNVCRLPG TPEALCATYT GCIIIPGATC PGDYAN

References

[1]	"Primary structure of the hydrophobic plant protein crambin." Teeter M.M., Mazer J.A., L'Italien J.J. Biochemistry 20:5437-5443(1981) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE.
[2]	"Structure of the hydrophobic protein crambin determined directly from the anomalous scattering of sulphur." Hendrickson W.A., Teeter M.M. Nature 290:107-113(1981) Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS), DISULFIDE BONDS.
[3]	"Correlated disorder of the pure Pro22/Leu25 form of crambin at 150 K refined to 1.05-A resolution." Yamano A., Teeter M.M. J. Biol. Chem. 269:13956-13965(1994) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.05 ANGSTROMS).
[4]	"Crystal structure of Ser-22/Ile-25 form crambin confirms solvent, side chain substate correlations." Yamano A., Heo N.-H., Teeter M.M. J. Biol. Chem. 272:9597-9600(1997) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (0.89 ANGSTROMS).
[5]	"Secondary structure and hydrogen bonding of crambin in solution. A two-dimensional NMR study." Lamerichs R.M.J.N., Berliner L.J., Boelens R., de Marco A., Llinas M., Kaptein R. Eur. J. Biochem. 171:307-312(1988) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Cross-references

Sequence databases

PIR

KECX. A01805.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1AB1	X-ray	0.89	A	1-46	[»]
1CBN	X-ray	0.83	A	1-46	[»]
1CCM	NMR	-	A	1-46	[»]
1CCN	NMR	-	A	1-46	[»]
1CNR	X-ray	1.05	A	1-46	[»]
1CRN	X-ray	1.50	A	1-46	[»]
1CXR	NMR	-	A	1-46	[»]
1EJG	X-ray	0.54	A	1-46	[»]
1JXT	X-ray	0.89	A	1-46	[»]
1JXU	X-ray	0.99	A	1-46	[»]
1JXW	X-ray	0.89	A	1-46	[»]
1JXX	X-ray	0.89	A	1-46	[»]
1JXY	X-ray	0.89	A	1-46	[»]
1YV8	NMR	-	A	1-46	[»]
1YVA	NMR	-	A	1-46	[»]
2EYA	NMR	-	A	1-46	[»]
2EYB	NMR	-	A	1-46	[»]
2EYC	NMR	-	A	1-46	[»]
2EYD	NMR	-	A	1-46	[»]
2FD7	X-ray	1.75	A	1-46	[»]
2FD9	X-ray	1.60	A	1-46	[»]
3NIR	X-ray	0.48	A	1-46	[»]
3U7T	X-ray	0.85	A	1-46	[»]
3UE7	X-ray	1.08	B	1-46	[»]
4FC1	neutron diffraction	1.10	A	1-46	[»]

ProteinModelPortal

P01542.

SMR

P01542. Positions 1-46.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

3.30.1350.10. 1 hit.

InterPro

IPR001010. Thionin.
[Graphical view]

Pfam

PF00321. Thionin. 1 hit.
[Graphical view]

PRINTS

PR00287. THIONIN.

SUPFAM

SSF57429. Thionin. 1 hit.

PROSITE

PS00271. THIONIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P01542.

Entry information

Entry name

CRAM_CRAAB

Accession

Primary (citable) accession number: P01542

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	May 30, 2000
Last modified:	April 3, 2013
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program