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P01542 (CRAM_CRAAB) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein attributes

Sequence length46 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The function of this hydrophobic plant seed protein is not known.

Subcellular location

Secreted.

Miscellaneous

Two isoforms exists, a major form PL (shown here) and a minor form SI.

Sequence similarities

Belongs to the plant thionin (TC 1.C.44) family. [View classification]

Ontologies

Keywords
   Biological processPlant defense
   Cellular componentSecreted
   PTMDisulfide bond
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Biological_processdefense response

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4646Crambin
PRO_0000221479

Amino acid modifications

Disulfide bond3 ↔ 40 Ref.2
Disulfide bond4 ↔ 32 Ref.2
Disulfide bond16 ↔ 26 Ref.2

Natural variations

Natural variant221P → S in isoform SI.
Natural variant251L → I in isoform SI.

Secondary structure

........... 46
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P01542 [UniParc].

Last modified May 30, 2000. Version 2.
Checksum: 919E68AF159EF722

FASTA464,736
        10         20         30         40 
TTCCPSIVAR SNFNVCRLPG TPEALCATYT GCIIIPGATC PGDYAN 

« Hide

References

[1]"Primary structure of the hydrophobic plant protein crambin."
Teeter M.M., Mazer J.A., L'Italien J.J.
Biochemistry 20:5437-5443(1981) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE.
[2]"Structure of the hydrophobic protein crambin determined directly from the anomalous scattering of sulphur."
Hendrickson W.A., Teeter M.M.
Nature 290:107-113(1981)
Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS), DISULFIDE BONDS.
[3]"Correlated disorder of the pure Pro22/Leu25 form of crambin at 150 K refined to 1.05-A resolution."
Yamano A., Teeter M.M.
J. Biol. Chem. 269:13956-13965(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.05 ANGSTROMS).
[4]"Crystal structure of Ser-22/Ile-25 form crambin confirms solvent, side chain substate correlations."
Yamano A., Heo N.-H., Teeter M.M.
J. Biol. Chem. 272:9597-9600(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (0.89 ANGSTROMS).
[5]"Secondary structure and hydrogen bonding of crambin in solution. A two-dimensional NMR study."
Lamerichs R.M.J.N., Berliner L.J., Boelens R., de Marco A., Llinas M., Kaptein R.
Eur. J. Biochem. 171:307-312(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR.
+Additional computationally mapped references.

Cross-references

Sequence databases

PIRKECX. A01805.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1AB1X-ray0.89A1-46[»]
1CBNX-ray0.83A1-46[»]
1CCMNMR-A1-46[»]
1CCNNMR-A1-46[»]
1CNRX-ray1.05A1-46[»]
1CRNX-ray1.50A1-46[»]
1CXRNMR-A1-46[»]
1EJGX-ray0.54A1-46[»]
1JXTX-ray0.89A1-46[»]
1JXUX-ray0.99A1-46[»]
1JXWX-ray0.89A1-46[»]
1JXXX-ray0.89A1-46[»]
1JXYX-ray0.89A1-46[»]
1YV8NMR-A1-46[»]
1YVANMR-A1-46[»]
2EYANMR-A1-46[»]
2EYBNMR-A1-46[»]
2EYCNMR-A1-46[»]
2EYDNMR-A1-46[»]
2FD7X-ray1.75A1-46[»]
2FD9X-ray1.60A1-46[»]
3NIRX-ray0.48A1-46[»]
3U7TX-ray0.85A1-46[»]
3UE7X-ray1.08B1-46[»]
4FC1neutron diffraction1.10A1-46[»]
ProteinModelPortalP01542.
SMRP01542. Positions 1-46.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.30.1350.10. 1 hit.
InterProIPR001010. Thionin.
[Graphical view]
PfamPF00321. Thionin. 1 hit.
[Graphical view]
PRINTSPR00287. THIONIN.
SUPFAMSSF57429. SSF57429. 1 hit.
PROSITEPS00271. THIONIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP01542.

Entry information

Entry nameCRAM_CRAAB
AccessionPrimary (citable) accession number: P01542
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: May 30, 2000
Last modified: February 19, 2014
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references