P01533 (TXA1_ANESU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Neurotoxin-1 Alternative name(s): As1 Toxin ATX-I
Organism	Anemonia sulcata (Mediterranean snakelocks sea anemone) (Anemonia viridis)
Taxonomic identifier	6108 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Cnidaria › Anthozoa › Hexacorallia › Actiniaria › Nynantheae › Actiniidae › Anemonia

Protein attributes

Sequence length	46 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds specifically to the sodium channel (Nav), thereby delaying its inactivation during signal transduction. Thus it strongly stimulates mammalian cardiac muscle contraction.
Subcellular location	Secreted. Nematocyst.
Sequence similarities	Belongs to the sea anemone sodium channel inhibitory toxin family. Type I subfamily.

Ontologies

Keywords
Cellular component	Nematocyst Secreted
Molecular function	Ion channel impairing toxin Neurotoxin Sodium channel inhibitor Toxin
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	regulation of signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell nematocyst Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	sodium channel inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 46

46

Neurotoxin-1

PRO_0000221512

Amino acid modifications

Disulfide bond

4 ↔ 43

Ref.2 Ref.3

Disulfide bond

6 ↔ 34

Ref.2 Ref.3

Disulfide bond

27 ↔ 44

Ref.2 Ref.3

Natural variations

Natural variant

3

1

A → P in about 20% of the molecules.

Secondary structure

1

.

.

.

.

.

.

.

.

.

46

Helix Strand Turn

Details...

Beta strand

3 – 5

3

Beta strand

16 – 25

10

Beta strand

32 – 34

3

Beta strand

37 – 45

9

Sequences

	Sequence		Length	Mass (Da)
	P01533 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 862C1E21FDA5432D	FASTA	46	4,814
	10 20 30 40 GAACLCKSDG PNTRGNSMSG TIWVFGCPSG WNNCEGRAII GYCCKQ

References

[1]	"Amino-acid sequence of toxin I from Anemonia sulcata." Wunderer G., Eulitz M. Eur. J. Biochem. 89:11-17(1978) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Nematoblast.
[2]	"The secondary structure of the toxin ATX Ia from Anemonia sulcata in aqueous solution determined on the basis of complete sequence-specific 1H-NMR assignments." Widmer H., Wagner G., Schweitz H., Lazdunski M., Wuethrich K. Eur. J. Biochem. 171:177-192(1988) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR, DISULFIDE BONDS.
[3]	"Three-dimensional structure of the neurotoxin ATX Ia from Anemonia sulcata in aqueous solution determined by nuclear magnetic resonance spectroscopy." Widmer H., Billeter M., Wuethrich K. Proteins 6:357-371(1989) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR, DISULFIDE BONDS.

Cross-references

Sequence databases

PIR

TZAZ1. A01796.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ATX	NMR	-	A	1-46	[»]

ProteinModelPortal

P01533.

SMR

P01533. Positions 1-46.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

2.20.20.10. 1 hit.

InterPro

IPR000693. Anenome_toxin.
IPR023355. Myo_neuro_toxin.
[Graphical view]

Pfam

PF00706. Toxin_4. 1 hit.
[Graphical view]

PIRSF

PIRSF001905. Anenome_toxin. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P01533.

Entry information

Entry name

TXA1_ANESU

Accession

Primary (citable) accession number: P01533

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	May 1, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Animal Toxin Annotation Program