##gff-version 3 P01531 UniProtKB Chain 1 49 . . . ID=PRO_0000221516;Note=Delta-actitoxin-Axm1b;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:4019448;Dbxref=PMID:4019448 P01531 UniProtKB Region 1 7 . . . Note=Well-structured region;Ontology_term=ECO:0000305,ECO:0000305;evidence=ECO:0000305|PubMed:14661964,ECO:0000305|PubMed:15170345;Dbxref=PMID:14661964,PMID:15170345 P01531 UniProtKB Region 8 17 . . . Note=Arg-14 loop (non-well-structured region);Ontology_term=ECO:0000305,ECO:0000305;evidence=ECO:0000305|PubMed:14661964,ECO:0000305|PubMed:15170345;Dbxref=PMID:14661964,PMID:15170345 P01531 UniProtKB Region 18 49 . . . Note=Well-structured region;Ontology_term=ECO:0000305,ECO:0000305;evidence=ECO:0000305|PubMed:14661964,ECO:0000305|PubMed:15170345;Dbxref=PMID:14661964,PMID:15170345 P01531 UniProtKB Site 7 7 . . . Note=Structurally important;Ontology_term=ECO:0000305;evidence=ECO:0000305|PubMed:8639500;Dbxref=PMID:8639500 P01531 UniProtKB Site 9 9 . . . Note=Important for sodium channel affinity and for toxin structure;Ontology_term=ECO:0000305;evidence=ECO:0000305|PubMed:8639500;Dbxref=PMID:8639500 P01531 UniProtKB Site 10 10 . . . Note=Important for affinity to sodium channel%2C probably due to the flexibility this residue gives to the Arg-14 loop;Ontology_term=ECO:0000305;evidence=ECO:0000305|PubMed:14661964;Dbxref=PMID:14661964 P01531 UniProtKB Site 12 12 . . . Note=Key residue for binding both cardiac and neuronal sodium channels;Ontology_term=ECO:0000305;evidence=ECO:0000305|PubMed:8276803;Dbxref=PMID:8276803 P01531 UniProtKB Site 13 13 . . . Note=Important for sodium channel affinity;Ontology_term=ECO:0000305;evidence=ECO:0000305|PubMed:8916901;Dbxref=PMID:8916901 P01531 UniProtKB Site 14 14 . . . Note=Not essential for sodium channel affinity;Ontology_term=ECO:0000305;evidence=ECO:0000305|PubMed:8288644;Dbxref=PMID:8288644 P01531 UniProtKB Site 15 15 . . . Note=Important for affinity to sodium channel%2C probably due to the flexibility this residue gives to the Arg-14 loop;Ontology_term=ECO:0000305;evidence=ECO:0000305|PubMed:14661964;Dbxref=PMID:14661964 P01531 UniProtKB Site 16 16 . . . Note=Binds to sodium channel;Ontology_term=ECO:0000305;evidence=ECO:0000305|PubMed:15170345;Dbxref=PMID:15170345 P01531 UniProtKB Site 17 17 . . . Note=Has its side chain oriented away from the channel in the binary complex;Ontology_term=ECO:0000305;evidence=ECO:0000305|PubMed:15170345;Dbxref=PMID:15170345 P01531 UniProtKB Site 18 18 . . . Note=Important for high affinity to sodium channel;Ontology_term=ECO:0000305;evidence=ECO:0000305|PubMed:8621610;Dbxref=PMID:8621610 P01531 UniProtKB Site 19 19 . . . Note=Binds to sodium channel;Ontology_term=ECO:0000305;evidence=ECO:0000305|PubMed:15170345;Dbxref=PMID:15170345 P01531 UniProtKB Site 20 20 . . . Note=Structurally important;Ontology_term=ECO:0000305;evidence=ECO:0000305|PubMed:14661964;Dbxref=PMID:14661964 P01531 UniProtKB Site 33 33 . . . Note=Important for channel affinity;Ontology_term=ECO:0000305;evidence=ECO:0000305|PubMed:8798612;Dbxref=PMID:8798612 P01531 UniProtKB Site 34 34 . . . Note=Not important for channel affinity and toxin structure;Ontology_term=ECO:0000305;evidence=ECO:0000305|PubMed:8639500;Dbxref=PMID:8639500 P01531 UniProtKB Site 37 37 . . . Note=Important for channel affinity (interacts with rat Nav1.5 channel residue Asp-1612);Ontology_term=ECO:0000305,ECO:0000305;evidence=ECO:0000305|PubMed:8639500,ECO:0000305|PubMed:9417050;Dbxref=PMID:8639500,PMID:9417050 P01531 UniProtKB Site 39 39 . . . Note=Not important for channel affinity and toxin structure;Ontology_term=ECO:0000305;evidence=ECO:0000305|PubMed:8639500;Dbxref=PMID:8639500 P01531 UniProtKB Site 43 43 . . . Note=Structurally important;Ontology_term=ECO:0000305;evidence=ECO:0000305|PubMed:8621610;Dbxref=PMID:8621610 P01531 UniProtKB Site 45 45 . . . Note=Does not affect binding%2C but may affect the stabilization of the cardiac channel open conformation;Ontology_term=ECO:0000305;evidence=ECO:0000305|PubMed:8798612;Dbxref=PMID:8798612 P01531 UniProtKB Site 48 48 . . . Note=Binds to sodium channel;Ontology_term=ECO:0000305;evidence=ECO:0000305|PubMed:8288644;Dbxref=PMID:8288644 P01531 UniProtKB Site 49 49 . . . Note=Important for most of the cardiac specificity;Ontology_term=ECO:0000305;evidence=ECO:0000305|PubMed:8276803;Dbxref=PMID:8276803 P01531 UniProtKB Disulfide bond 4 46 . . . Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:7582896;Dbxref=PMID:7582896 P01531 UniProtKB Disulfide bond 6 36 . . . Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:7582896;Dbxref=PMID:7582896 P01531 UniProtKB Disulfide bond 29 47 . . . Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:7582896;Dbxref=PMID:7582896 P01531 UniProtKB Mutagenesis 3 3 . . . Note=Minor decrease in affinity for sodium channels (4.7-fold on neuronal and 2-fold on cardiac (Nav1.5) channels). P->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8916901;Dbxref=PMID:8916901 P01531 UniProtKB Mutagenesis 7 7 . . . Note=Incorrect folding or very limited amount of mutant obtained. D->A%2CN;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8639500;Dbxref=PMID:8639500 P01531 UniProtKB Mutagenesis 7 7 . . . Note=Incorrect folding%3B when associated with D-37. D->K;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8639500;Dbxref=PMID:8639500 P01531 UniProtKB Mutagenesis 7 7 . . . Note=Small decrease in affinity (4-6-fold)%2C and very limited amount of mutant obtained. D->N;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8639500;Dbxref=PMID:8639500 P01531 UniProtKB Mutagenesis 9 9 . . . Note=Major decrease in affinity for both cardiac (Nav1.5) (300-fold) and neuronal (100-fold) channels. D->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8639500;Dbxref=PMID:8639500 P01531 UniProtKB Mutagenesis 9 9 . . . Note=Decrease in affinity for both cardiac (Nav1.5) (10-fold) and neuronal (8-fold) channels. D->N;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8639500;Dbxref=PMID:8639500 P01531 UniProtKB Mutagenesis 10 10 . . . Note=Decrease in affinity for both cardiac (Nav1.5) (15-fold) and neuronal (450-fold) channels%2C as well as a 30-fold increase in discrimination for Nav1.5. Decrease in affinity for cardiac (Nav1.5) (600-fold)%3B when associated with A-15. Not correctly folded%3B when associated with A-20. G->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:14661964;Dbxref=PMID:14661964 P01531 UniProtKB Mutagenesis 12 12 . . . Note=Major decrease in affinity for both cardiac (Nav1.5) and neuronal sodium channels. R->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8276803;Dbxref=PMID:8276803 P01531 UniProtKB Mutagenesis 12 12 . . . Note=Minor effect on toxicity. R->K;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8276803;Dbxref=PMID:8276803 P01531 UniProtKB Mutagenesis 12 12 . . . Note=Minor effect on toxicity. Decrease in affinity for both cardiac (Nav1.5) (5-fold) and neuronal (37-fold) channels%3B when associated with Q-49 (tested by ion flux studies). Loss of discrimination between cardiac and neuronal channels%3B when associated with Val-13 and Q-49. R->S;Ontology_term=ECO:0000269,ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:7612595,ECO:0000269|PubMed:8276803,ECO:0000269|PubMed:8916901;Dbxref=PMID:7612595,PMID:8276803,PMID:8916901 P01531 UniProtKB Mutagenesis 13 13 . . . Note=Decrease in affinity for both cardiac (Nav1.5) (9-fold) and neuronal channels (9-fold). Loss of discrimination between cardiac and neuronal channels%3B when associated with S-12 and Q-49. P->V;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8916901;Dbxref=PMID:8916901 P01531 UniProtKB Mutagenesis 14 14 . . . Note=Minor effect on toxicity. R->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8288644;Dbxref=PMID:8288644 P01531 UniProtKB Mutagenesis 14 14 . . . Note=Minor effect on toxicity. R->K;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8288644;Dbxref=PMID:8288644 P01531 UniProtKB Mutagenesis 14 14 . . . Note=Minor effect on toxicity. Decrease in affinity for both cardiac (Nav1.5) (56-fold) and neuronal (72-fold) channels%3B when associated with S-12 (tested by ion flux studies). Decrease in affinity for both cardiac (Nav1.5) (13-fold) and neuronal (27-fold) channels%3B when associated with A-48 (tested by ion flux studies). R->Q;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:7612595,ECO:0000269|PubMed:8288644;Dbxref=PMID:7612595,PMID:8288644 P01531 UniProtKB Mutagenesis 15 15 . . . Note=Decrease in affinity for both cardiac (Nav1.5) (13-fold) and neuronal (600-fold) channels%2C as well as a 50-fold increase in discrimination for Nav1.5. Decrease in affinity for cardiac (Nav1.5) (600-fold)%3B when associated with A-10. G->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:14661964;Dbxref=PMID:14661964 P01531 UniProtKB Mutagenesis 16 16 . . . Note=Decrease in affinity for cardiac (Nav1.5) (8-fold) channels. N->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:15170345;Dbxref=PMID:15170345 P01531 UniProtKB Mutagenesis 16 16 . . . Note=Decrease in affinity for both cardiac (Nav1.5) (500-fold) and neuronal (3600-fold) channels. N->D;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:15170345;Dbxref=PMID:15170345 P01531 UniProtKB Mutagenesis 16 16 . . . Note=Decrease in affinity for both cardiac (Nav1.5) (5-fold) and neuronal (56-fold) channels. N->R;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:15170345;Dbxref=PMID:15170345 P01531 UniProtKB Mutagenesis 17 17 . . . Note=No change in activity. T->A%2CD;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:15170345;Dbxref=PMID:15170345 P01531 UniProtKB Mutagenesis 18 18 . . . Note=Major decrease in affinity for both cardiac (Nav1.5) (330-fold) and neuronal (34-fold) channels%2C as well as a 9.5-fold decrease in discrimination for Nav1.5. L->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8621610;Dbxref=PMID:8621610 P01531 UniProtKB Mutagenesis 18 18 . . . Note=Decrease in affinity for both cardiac (Nav1.5) and neuronal channels. L->V;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8621610;Dbxref=PMID:8621610 P01531 UniProtKB Mutagenesis 19 19 . . . Note=Decrease in affinity for cardiac (Nav1.5) (5.6-fold) channels. S->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:15170345;Dbxref=PMID:15170345 P01531 UniProtKB Mutagenesis 19 19 . . . Note=Major decrease in affinity for both cardiac (Nav1.5) (85-fold) and neuronal (653-fold) channels. S->D;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:15170345;Dbxref=PMID:15170345 P01531 UniProtKB Mutagenesis 19 19 . . . Note=Decrease in affinity for both cardiac (Nav1.5) (5.7-fold) and neuronal (27-fold) channels. S->R;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:15170345;Dbxref=PMID:15170345 P01531 UniProtKB Mutagenesis 20 20 . . . Note=Incorrect folding. Incorrect folding%3B when associated with A-10. G->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:14661964;Dbxref=PMID:14661964 P01531 UniProtKB Mutagenesis 21 21 . . . Note=Minor decrease in affinity for sodium channels (2.2-fold on neuronal and 2.9-fold on cardiac (Nav1.5) channels). I->T;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8916901;Dbxref=PMID:8916901 P01531 UniProtKB Mutagenesis 24 24 . . . Note=Minor decrease in affinity for sodium channels (4.8-fold on neuronal and 2.4-fold on cardiac (Nav1.5) channels). F->L;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8916901;Dbxref=PMID:8916901 P01531 UniProtKB Mutagenesis 33 33 . . . Note=No mutant obtained. W->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8798612;Dbxref=PMID:8798612 P01531 UniProtKB Mutagenesis 33 33 . . . Note=Major decrease in affinity for both cardiac (Nav1.5) (31-fold) and neuronal (50-fold) channels (tested by ion flux studies). This mutant is the first ApB mutant that displays a significantly altered association rate (K(on)). W->F;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8798612;Dbxref=PMID:8798612 P01531 UniProtKB Mutagenesis 33 33 . . . Note=No mutant obtained. W->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8798612;Dbxref=PMID:8798612 P01531 UniProtKB Mutagenesis 33 33 . . . Note=Minor decrease in affinity for both cardiac (Nav1.5) (5.6-fold) and neuronal (5-fold) channels. W->Y;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8798612;Dbxref=PMID:8798612 P01531 UniProtKB Mutagenesis 34 34 . . . Note=Minor decrease in affinity. H->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8639500;Dbxref=PMID:8639500 P01531 UniProtKB Mutagenesis 37 37 . . . Note=Decrease in affinity for both cardiac (Nav1.5) (11-fold) and neuronal (7-fold) channels. K->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8639500;Dbxref=PMID:8639500 P01531 UniProtKB Mutagenesis 37 37 . . . Note=Decrease in affinity for cardiac (Nav1.5) channels (13-fold) (with decrease in K(on) and increase in K(off)). K->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:9417050;Dbxref=PMID:9417050 P01531 UniProtKB Mutagenesis 37 37 . . . Note=Incorrect folding%3B when associated with K-7. K->D;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8639500;Dbxref=PMID:8639500 P01531 UniProtKB Mutagenesis 39 39 . . . Note=No change in activity. H->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8639500;Dbxref=PMID:8639500 P01531 UniProtKB Mutagenesis 39 39 . . . Note=Small decrease in affinity for cardiac (Nav1.5) channels (1.1-fold) (with increase in both K(on) and K(off)). H->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:9417050;Dbxref=PMID:9417050 P01531 UniProtKB Mutagenesis 42 42 . . . Note=Minor decrease in affinity for sodium channels (1.1-fold on neuronal and 3.4-fold on cardiac (Nav1.5) channels). N->T;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8916901;Dbxref=PMID:8916901 P01531 UniProtKB Mutagenesis 43 43 . . . Note=Incorrect folding. I->A%2CG%2CF;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8621610;Dbxref=PMID:8621610 P01531 UniProtKB Mutagenesis 43 43 . . . Note=Small decrease in apparent binding affinity for both neuronal and cardiac (Nav1.5) channels (tested by ion flux studies). I->L%2CV;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8621610;Dbxref=PMID:8621610 P01531 UniProtKB Mutagenesis 45 45 . . . Note=Minor decrease in affinity for both cardiac (Nav1.5) (7.7-fold) and neuronal (4-fold) channels (tested by ion flux studies). W->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8798612;Dbxref=PMID:8798612 P01531 UniProtKB Mutagenesis 45 45 . . . Note=Minor decrease in affinity for both cardiac (Nav1.5) (2-4-fold) (with decrease in K(on) and increase in K(off)) and neuronal (5-fold) channels (tested by ion flux studies). W->F;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:8798612,ECO:0000269|PubMed:9417050;Dbxref=PMID:8798612,PMID:9417050 P01531 UniProtKB Mutagenesis 45 45 . . . Note=Minor decrease in affinity for both cardiac (Nav1.5) (3.3-fold) and neuronal (7-fold) channels (tested by ion flux studies). W->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8798612;Dbxref=PMID:8798612 P01531 UniProtKB Mutagenesis 48 48 . . . Note=Minor effect on toxicity. Decrease in affinity for both cardiac (Nav1.5) (13-fold) and neuronal (27-fold) channels%3B when associated with Q-14 (tested by ion flux studies). K->A;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:7612595,ECO:0000269|PubMed:8288644;Dbxref=PMID:7612595,PMID:8288644 P01531 UniProtKB Mutagenesis 48 48 . . . Note=Minor effect on toxicity. K->Q;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8288644;Dbxref=PMID:8288644 P01531 UniProtKB Mutagenesis 48 48 . . . Note=Minor effect on toxicity. K->R;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8288644;Dbxref=PMID:8288644 P01531 UniProtKB Mutagenesis 49 49 . . . Note=Minor effect on toxicity. K->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8276803;Dbxref=PMID:8276803 P01531 UniProtKB Mutagenesis 49 49 . . . Note=Minor effect on toxicity. Decrease in affinity for both cardiac (Nav1.5) (5-fold) and neuronal (37-fold) channels%3B when associated with S-12 (tested by ion flux studies). Loss of discrimination between cardiac and neuronal channels%3B when associated with S-12 and V-13. K->Q;Ontology_term=ECO:0000269,ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:7612595,ECO:0000269|PubMed:8276803,ECO:0000269|PubMed:8916901;Dbxref=PMID:7612595,PMID:8276803,PMID:8916901 P01531 UniProtKB Mutagenesis 49 49 . . . Note=Minor effect on toxicity. K->R;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8276803;Dbxref=PMID:8276803 P01531 UniProtKB Sequence conflict 12 13 . . . Note=RP->PN;Ontology_term=ECO:0000305;evidence=ECO:0000305 P01531 UniProtKB Sequence conflict 25 25 . . . Note=Y->A;Ontology_term=ECO:0000305;evidence=ECO:0000305 P01531 UniProtKB Turn 14 17 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1APF P01531 UniProtKB Beta strand 20 23 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1APF P01531 UniProtKB Beta strand 42 47 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1APF