Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P01531 (TXAB_ANTXA) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Anthopleurin-B

Short name=AP-B
Short name=ApB
OrganismAnthopleura xanthogrammica (Giant green sea anemone)
Taxonomic identifier6112 [NCBI]
Taxonomic lineageEukaryotaMetazoaCnidariaAnthozoaHexacoralliaActiniariaNynantheaeActiniidaeAnthopleura

Protein attributes

Sequence length49 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds specifically to the sodium channel (site 3) (Nav), thereby delaying its inactivation during signal transduction. Thus it strongly stimulates mammalian cardiac muscle contraction. Does not display phospholipid-binding activity. Anthopleurin-B is the most potent peptide heart stimulator isolated from the sea anemone.

Subcellular location

Secreted. Nematocyst.

Sequence similarities

Belongs to the sea anemone sodium channel inhibitory toxin family. Type I subfamily.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4949Anthopleurin-B
PRO_0000221516

Sites

Site161Binds to sodium channel
Site181Binds to sodium channel
Site191Binds to sodium channel
Site331Binds to sodium channel
Site371Binds to sodium channel

Amino acid modifications

Disulfide bond4 ↔ 46 Ref.8
Disulfide bond6 ↔ 36 Ref.8
Disulfide bond29 ↔ 47 Ref.8

Experimental info

Sequence conflict12 – 132RP → PN AA sequence Ref.2
Sequence conflict251Y → A AA sequence Ref.2

Secondary structure

....... 49
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P01531 [UniParc].

Last modified January 1, 1988. Version 1.
Checksum: 7BD237179065AE90

FASTA495,274
        10         20         30         40 
GVPCLCDSDG PRPRGNTLSG ILWFYPSGCP SGWHNCKAHG PNIGWCCKK 

« Hide

References

[1]"Amino acid sequence of the Anthopleura xanthogrammica heart stimulant, anthopleurin-B."
Reimer N.S., Yasunobu C.L., Yasunobu K.T., Norton T.R.
J. Biol. Chem. 260:8690-8693(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE.
Tissue: Nematoblast.
[2]"Cardiotonic polypeptides from Anthopleura xanthogrammica (Brandt) and A. elegantissima (Brandt)."
Norton T.R.
Fed. Proc. 40:21-25(1981) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-28.
[3]"Leucine 18, a hydrophobic residue essential for high affinity binding of anthopleurin B to the voltage-sensitive sodium channel."
Dias-Kadambi B.L., Drum C.L., Hanck D.A., Blumenthal K.M.
J. Biol. Chem. 271:9422-9428(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: SODIUM CHANNEL-BINDING SITE LEU-18.
[4]"A specific interaction between the cardiac sodium channel and site-3 toxin anthopleurin B."
Benzinger G.R., Kyle J.W., Blumenthal K.M., Hanck D.A.
J. Biol. Chem. 273:80-84(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: SODIUM CHANNEL-BINDING SITE LYS-37.
[5]"The role of exposed tryptophan residues in the activity of the cardiotonic polypeptide anthopleurin B."
Dias-Kadambi B.L., Combs K.A., Drum C.L., Hanck D.A., Blumenthal K.M.
J. Biol. Chem. 271:23828-23835(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: SODIUM CHANNEL-BINDING SITE TRP-33.
[6]"Role of Asn-16 and Ser-19 in anthopleurin B binding. Implications for the electrostatic nature of Na(V) site 3."
Seibert A.L., Liu J., Hanck D.A., Blumenthal K.M.
Biochemistry 43:7082-7089(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SODIUM CHANNEL-BINDING SITES ASN-16 AND SER-19.
[7]"Differential phospholipid binding by site 3 and site 4 toxins. Implications for structural variability between voltage-sensitive sodium channel domains."
Smith J.J., Alphy S., Seibert A.L., Blumenthal K.M.
J. Biol. Chem. 280:11127-11133(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHOLIPID-BINDING ACTIVITY.
[8]"Solution structure of the cardiostimulant polypeptide anthopleurin-B and comparison with anthopleurin-A."
Monks S.A., Pallaghy P.K., Scanlon M.J., Norton R.S.
Structure 3:791-803(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR, DISULFIDE BONDS.

Cross-references

Sequence databases

PIRNAXAB. A92547.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1APFNMR-A1-49[»]
ProteinModelPortalP01531.
SMRP01531. Positions 1-49.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.20.20.10. 1 hit.
InterProIPR000693. Anenome_toxin.
IPR023355. Myo_neuro_toxin.
[Graphical view]
PfamPF00706. Toxin_4. 1 hit.
[Graphical view]
PIRSFPIRSF001905. Anenome_toxin. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP01531.

Entry information

Entry nameTXAB_ANTXA
AccessionPrimary (citable) accession number: P01531
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 1, 1988
Last modified: October 16, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references