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Protein

Anthopleurin-B

Gene
N/A
Organism
Anthopleura xanthogrammica (Giant green sea anemone)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Binds specifically to the sodium channel (site 3) (Nav), thereby delaying its inactivation during signal transduction. Thus it strongly stimulates mammalian cardiac muscle contraction. Does not display phospholipid-binding activity. Anthopleurin-B is the most potent peptide heart stimulator isolated from the sea anemone.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei16 – 161Binds to sodium channel
Sitei18 – 181Binds to sodium channel
Sitei19 – 191Binds to sodium channel
Sitei33 – 331Binds to sodium channel
Sitei37 – 371Binds to sodium channel

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel impairing toxin, Neurotoxin, Toxin, Voltage-gated sodium channel impairing toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Anthopleurin-B
Short name:
AP-B
Short name:
ApB
OrganismiAnthopleura xanthogrammica (Giant green sea anemone)
Taxonomic identifieri6112 [NCBI]
Taxonomic lineageiEukaryotaMetazoaCnidariaAnthozoaHexacoralliaActiniariaNynantheaeActiniidaeAnthopleura

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nematocyst, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4949Anthopleurin-BPRO_0000221516Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi4 ↔ 461 Publication
Disulfide bondi6 ↔ 361 Publication
Disulfide bondi29 ↔ 471 Publication

Keywords - PTMi

Disulfide bond

Structurei

Secondary structure

1
49
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni14 – 174Combined sources
Beta strandi20 – 234Combined sources
Beta strandi42 – 476Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1APFNMR-A1-49[»]
ProteinModelPortaliP01531.
SMRiP01531. Positions 1-49.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP01531.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di2.20.20.10. 1 hit.
InterProiIPR000693. Anenome_toxin.
IPR023355. Myo_neuro_toxin.
[Graphical view]
PfamiPF00706. Toxin_4. 1 hit.
[Graphical view]
PIRSFiPIRSF001905. Anenome_toxin. 1 hit.

Sequencei

Sequence statusi: Complete.

P01531-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40 
GVPCLCDSDG PRPRGNTLSG ILWFYPSGCP SGWHNCKAHG PNIGWCCKK
Length:49
Mass (Da):5,274
Last modified:January 1, 1988 - v1
Checksum:i7BD237179065AE90
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti12 – 132RP → PN AA sequence (PubMed:6108877).Curated
Sequence conflicti25 – 251Y → A AA sequence (PubMed:6108877).Curated

Sequence databases

PIRiA92547. NAXAB.

Cross-referencesi

Sequence databases

PIRiA92547. NAXAB.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1APFNMR-A1-49[»]
ProteinModelPortaliP01531.
SMRiP01531. Positions 1-49.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP01531.

Family and domain databases

Gene3Di2.20.20.10. 1 hit.
InterProiIPR000693. Anenome_toxin.
IPR023355. Myo_neuro_toxin.
[Graphical view]
PfamiPF00706. Toxin_4. 1 hit.
[Graphical view]
PIRSFiPIRSF001905. Anenome_toxin. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Amino acid sequence of the Anthopleura xanthogrammica heart stimulant, anthopleurin-B."
    Reimer N.S., Yasunobu C.L., Yasunobu K.T., Norton T.R.
    J. Biol. Chem. 260:8690-8693(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.
    Tissue: Nematoblast.
  2. "Cardiotonic polypeptides from Anthopleura xanthogrammica (Brandt) and A. elegantissima (Brandt)."
    Norton T.R.
    Fed. Proc. 40:21-25(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-28.
  3. "Leucine 18, a hydrophobic residue essential for high affinity binding of anthopleurin B to the voltage-sensitive sodium channel."
    Dias-Kadambi B.L., Drum C.L., Hanck D.A., Blumenthal K.M.
    J. Biol. Chem. 271:9422-9428(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: SODIUM CHANNEL-BINDING SITE LEU-18.
  4. "A specific interaction between the cardiac sodium channel and site-3 toxin anthopleurin B."
    Benzinger G.R., Kyle J.W., Blumenthal K.M., Hanck D.A.
    J. Biol. Chem. 273:80-84(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: SODIUM CHANNEL-BINDING SITE LYS-37.
  5. "The role of exposed tryptophan residues in the activity of the cardiotonic polypeptide anthopleurin B."
    Dias-Kadambi B.L., Combs K.A., Drum C.L., Hanck D.A., Blumenthal K.M.
    J. Biol. Chem. 271:23828-23835(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: SODIUM CHANNEL-BINDING SITE TRP-33.
  6. "Role of Asn-16 and Ser-19 in anthopleurin B binding. Implications for the electrostatic nature of Na(V) site 3."
    Seibert A.L., Liu J., Hanck D.A., Blumenthal K.M.
    Biochemistry 43:7082-7089(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SODIUM CHANNEL-BINDING SITES ASN-16 AND SER-19.
  7. "Differential phospholipid binding by site 3 and site 4 toxins. Implications for structural variability between voltage-sensitive sodium channel domains."
    Smith J.J., Alphy S., Seibert A.L., Blumenthal K.M.
    J. Biol. Chem. 280:11127-11133(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHOLIPID-BINDING ACTIVITY.
  8. "Solution structure of the cardiostimulant polypeptide anthopleurin-B and comparison with anthopleurin-A."
    Monks S.A., Pallaghy P.K., Scanlon M.J., Norton R.S.
    Structure 3:791-803(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR, DISULFIDE BONDS.

Entry informationi

Entry nameiTXAB_ANTXA
AccessioniPrimary (citable) accession number: P01531
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 1, 1988
Last modified: January 7, 2015
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.