Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P01531

- TXAB_ANTXA

UniProt

P01531 - TXAB_ANTXA

Protein

Anthopleurin-B

Gene
N/A
Organism
Anthopleura xanthogrammica (Giant green sea anemone)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Binds specifically to the sodium channel (site 3) (Nav), thereby delaying its inactivation during signal transduction. Thus it strongly stimulates mammalian cardiac muscle contraction. Does not display phospholipid-binding activity. Anthopleurin-B is the most potent peptide heart stimulator isolated from the sea anemone.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei16 – 161Binds to sodium channel
    Sitei18 – 181Binds to sodium channel
    Sitei19 – 191Binds to sodium channel
    Sitei33 – 331Binds to sodium channel
    Sitei37 – 371Binds to sodium channel

    GO - Biological processi

    1. regulation of signal transduction Source: InterPro

    Keywords - Molecular functioni

    Ion channel impairing toxin, Neurotoxin, Toxin, Voltage-gated sodium channel impairing toxin

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Anthopleurin-B
    Short name:
    AP-B
    Short name:
    ApB
    OrganismiAnthopleura xanthogrammica (Giant green sea anemone)
    Taxonomic identifieri6112 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaCnidariaAnthozoaHexacoralliaActiniariaNynantheaeActiniidaeAnthopleura

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell
    2. nematocyst Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nematocyst, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 4949Anthopleurin-BPRO_0000221516Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi4 ↔ 461 Publication
    Disulfide bondi6 ↔ 361 Publication
    Disulfide bondi29 ↔ 471 Publication

    Keywords - PTMi

    Disulfide bond

    Structurei

    Secondary structure

    1
    49
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni14 – 174
    Beta strandi20 – 234
    Beta strandi42 – 476

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1APFNMR-A1-49[»]
    ProteinModelPortaliP01531.
    SMRiP01531. Positions 1-49.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP01531.

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di2.20.20.10. 1 hit.
    InterProiIPR000693. Anenome_toxin.
    IPR023355. Myo_neuro_toxin.
    [Graphical view]
    PfamiPF00706. Toxin_4. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001905. Anenome_toxin. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P01531-1 [UniParc]FASTAAdd to Basket

    « Hide

    GVPCLCDSDG PRPRGNTLSG ILWFYPSGCP SGWHNCKAHG PNIGWCCKK    49
    Length:49
    Mass (Da):5,274
    Last modified:January 1, 1988 - v1
    Checksum:i7BD237179065AE90
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti12 – 132RP → PN AA sequence (PubMed:6108877)Curated
    Sequence conflicti25 – 251Y → A AA sequence (PubMed:6108877)Curated

    Sequence databases

    PIRiA92547. NAXAB.

    Cross-referencesi

    Sequence databases

    PIRi A92547. NAXAB.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1APF NMR - A 1-49 [» ]
    ProteinModelPortali P01531.
    SMRi P01531. Positions 1-49.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P01531.

    Family and domain databases

    Gene3Di 2.20.20.10. 1 hit.
    InterProi IPR000693. Anenome_toxin.
    IPR023355. Myo_neuro_toxin.
    [Graphical view ]
    Pfami PF00706. Toxin_4. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001905. Anenome_toxin. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Amino acid sequence of the Anthopleura xanthogrammica heart stimulant, anthopleurin-B."
      Reimer N.S., Yasunobu C.L., Yasunobu K.T., Norton T.R.
      J. Biol. Chem. 260:8690-8693(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE.
      Tissue: Nematoblast.
    2. "Cardiotonic polypeptides from Anthopleura xanthogrammica (Brandt) and A. elegantissima (Brandt)."
      Norton T.R.
      Fed. Proc. 40:21-25(1981) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-28.
    3. "Leucine 18, a hydrophobic residue essential for high affinity binding of anthopleurin B to the voltage-sensitive sodium channel."
      Dias-Kadambi B.L., Drum C.L., Hanck D.A., Blumenthal K.M.
      J. Biol. Chem. 271:9422-9428(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: SODIUM CHANNEL-BINDING SITE LEU-18.
    4. "A specific interaction between the cardiac sodium channel and site-3 toxin anthopleurin B."
      Benzinger G.R., Kyle J.W., Blumenthal K.M., Hanck D.A.
      J. Biol. Chem. 273:80-84(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: SODIUM CHANNEL-BINDING SITE LYS-37.
    5. "The role of exposed tryptophan residues in the activity of the cardiotonic polypeptide anthopleurin B."
      Dias-Kadambi B.L., Combs K.A., Drum C.L., Hanck D.A., Blumenthal K.M.
      J. Biol. Chem. 271:23828-23835(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: SODIUM CHANNEL-BINDING SITE TRP-33.
    6. "Role of Asn-16 and Ser-19 in anthopleurin B binding. Implications for the electrostatic nature of Na(V) site 3."
      Seibert A.L., Liu J., Hanck D.A., Blumenthal K.M.
      Biochemistry 43:7082-7089(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SODIUM CHANNEL-BINDING SITES ASN-16 AND SER-19.
    7. "Differential phospholipid binding by site 3 and site 4 toxins. Implications for structural variability between voltage-sensitive sodium channel domains."
      Smith J.J., Alphy S., Seibert A.L., Blumenthal K.M.
      J. Biol. Chem. 280:11127-11133(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHOLIPID-BINDING ACTIVITY.
    8. "Solution structure of the cardiostimulant polypeptide anthopleurin-B and comparison with anthopleurin-A."
      Monks S.A., Pallaghy P.K., Scanlon M.J., Norton R.S.
      Structure 3:791-803(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR, DISULFIDE BONDS.

    Entry informationi

    Entry nameiTXAB_ANTXA
    AccessioniPrimary (citable) accession number: P01531
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: January 1, 1988
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programAnimal Toxin Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3