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Protein

Neurotoxin B-IV

Gene
N/A
Organism
Cerebratulus lacteus (Milky ribbon worm) (Micrura lactea)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This toxin increases the excitability of nerves by delaying the inactivation of the voltage-gated sodium channel (Nav). Only acts on some crustacean. Neurotoxin B-IV is more abundant, but 15-fold less toxic than neurotoxin B-II.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel impairing toxin, Toxin, Voltage-gated sodium channel impairing toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Neurotoxin B-IV
OrganismiCerebratulus lacteus (Milky ribbon worm) (Micrura lactea)
Taxonomic identifieri6221 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaNemerteaAnoplaHeteronemerteaCerebratulidaeCerebratulus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi9Y → F: 5-fold decrease in toxicity. 1 Publication1
Mutagenesisi13E → A: No change in toxicity. 1 Publication1
Mutagenesisi13E → Q: No change in toxicity. 1 Publication1
Mutagenesisi17R → A: Complete loss of toxicity. 1 Publication1
Mutagenesisi17R → K: Complete loss of toxicity. 1 Publication1
Mutagenesisi17R → Q: Complete loss of toxicity. 1 Publication1
Mutagenesisi18 – 19KK → QQ: Very low decrease in toxicity. 1 Publication2
Mutagenesisi18K → Q: No change in toxicity. 1 Publication1
Mutagenesisi21D → A: No change in toxicity. 1 Publication1
Mutagenesisi21D → N: No change in toxicity. 1 Publication1
Mutagenesisi21D → P: 10-fold decrease in toxicity. 1 Publication1
Mutagenesisi22L → D: No change in toxicity. 1 Publication1
Mutagenesisi25R → K: No change in toxicity. 1 Publication1
Mutagenesisi25R → Q: 400-fold decrease in toxicity. 1 Publication1
Mutagenesisi29K → N: No change in toxicity. 1 Publication1
Mutagenesisi30W → F: No change in toxicity. 1 Publication1
Mutagenesisi30W → S: 41-fold decrease in toxicity. 1 Publication1
Mutagenesisi30W → Y: 5-fold decrease in toxicity. 1 Publication1
Mutagenesisi33K → N: No decrease in toxicity. 1 Publication1
Mutagenesisi34R → A: 80-fold decrease in toxicity. 1 Publication1
Mutagenesisi34R → K: 8-fold decrease in toxicity. 1 Publication1
Mutagenesisi34R → Q: 20-fold decrease in toxicity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002215721 – 55Neurotoxin B-IVAdd BLAST55

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei10Hydroxyproline1 Publication1
Disulfide bondi12 ↔ 481 Publication
Disulfide bondi16 ↔ 521 Publication
Disulfide bondi23 ↔ 411 Publication
Disulfide bondi26 ↔ 371 Publication

Keywords - PTMi

Disulfide bond, Hydroxylation

Structurei

Secondary structure

155
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni7 – 10Combined sources4
Helixi11 – 23Combined sources13
Helixi28 – 30Combined sources3
Helixi34 – 48Combined sources15
Turni49 – 51Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VIBNMR-A1-55[»]
ProteinModelPortaliP01525.
SMRiP01525.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP01525.

Family & Domainsi

Sequence similaritiesi

Belongs to the worm B-toxin family.Curated

Family and domain databases

Gene3Di1.10.287.120. 1 hit.
InterProiIPR012497. Neurotoxin_B-IV.
[Graphical view]
PfamiPF07822. Toxin_13. 1 hit.
[Graphical view]
SUPFAMiSSF57011. SSF57011. 1 hit.

Sequencei

Sequence statusi: Complete.

P01525-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
ASATWGAAYP ACENNCRKKY DLCIRCQGKW AGKRGKCAAH CIIQKNNCKG

KCKKE
Length:55
Mass (Da):6,107
Last modified:July 21, 1986 - v1
Checksum:iBB76B72E48DB050D
GO

Sequence databases

PIRiA92340. NTHNB4.

Cross-referencesi

Sequence databases

PIRiA92340. NTHNB4.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VIBNMR-A1-55[»]
ProteinModelPortaliP01525.
SMRiP01525.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP01525.

Family and domain databases

Gene3Di1.10.287.120. 1 hit.
InterProiIPR012497. Neurotoxin_B-IV.
[Graphical view]
PfamiPF07822. Toxin_13. 1 hit.
[Graphical view]
SUPFAMiSSF57011. SSF57011. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNXB4_CERLA
AccessioniPrimary (citable) accession number: P01525
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 2, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.