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Protein

Omega-conotoxin GVIA

Gene
N/A
Organism
Conus geographus (Geography cone) (Nubecula geographus)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Omega-conotoxins act at presynaptic membranes, they bind and block voltage-gated calcium channels (Cav).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Calcium channel impairing toxin, Ion channel impairing toxin, Neurotoxin, Presynaptic neurotoxin, Toxin, Voltage-gated calcium channel impairing toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Omega-conotoxin GVIA
Alternative name(s):
SNX-124
Shaker peptide
Cleaved into the following 2 chains:
OrganismiConus geographus (Geography cone) (Nubecula geographus)
Taxonomic identifieri6491 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaCaenogastropodaHypsogastropodaNeogastropodaConoideaConidaeConus

Organism-specific databases

ConoServeri820. GVIA precursor.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi47 – 471K → A: Strong decrease in activity. 2 Publications
Mutagenesisi58 – 581Y → A: Strong decrease in activity. 2 Publications
Mutagenesisi58 – 581Y → F: Decrease in affinity. 2 Publications
Mutagenesisi62 – 621R → A: Decrease in potency, but not in affinity. 2 Publications
Mutagenesisi67 – 671Y → A: Decrease in potency, but not in affinity. 1 Publication
Mutagenesisi69 – 691K → A: Decrease in potency, but not in affinity. 2 Publications
Mutagenesisi70 – 701R → A: No change in activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence analysisAdd
BLAST
Propeptidei23 – 45232 PublicationsPRO_0000034906Add
BLAST
Peptidei46 – 7328Omega-conotoxin GVIBPRO_0000034907Add
BLAST
Peptidei46 – 7227Omega-conotoxin GVIAPRO_0000034908Add
BLAST
Peptidei46 – 7126Omega-conotoxin GVICPRO_0000034909Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi46 ↔ 611 Publication
Modified residuei49 – 4914-hydroxyproline1 Publication
Disulfide bondi53 ↔ 641 Publication
Modified residuei55 – 5514-hydroxyproline1 Publication
Disulfide bondi60 ↔ 711 Publication
Modified residuei66 – 6614-hydroxyproline1 Publication
Modified residuei72 – 721Tyrosine amide; in form omega-conotoxin GVIA1 Publication

Keywords - PTMi

Amidation, Disulfide bond, Hydroxylation

Expressioni

Tissue specificityi

Expressed by the venom duct.

Interactioni

Protein-protein interaction databases

IntActiP01522. 1 interaction.

Structurei

Secondary structure

1
73
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni55 – 584Combined sources
Beta strandi60 – 623Combined sources
Turni66 – 683Combined sources
Beta strandi69 – 713Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OMCNMR-A46-72[»]
1TR6NMR-A46-72[»]
1TTLNMR-A46-72[»]
2CCONMR-A46-72[»]
ProteinModelPortaliP01522.
SMRiP01522. Positions 46-72.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP01522.

Family & Domainsi

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.
The cysteine framework is VI/VII (C-C-CC-C-C).

Sequence similaritiesi

Belongs to the conotoxin O1 superfamily.Curated

Keywords - Domaini

Knottin, Signal

Family and domain databases

InterProiIPR004214. Conotoxin.
IPR012321. Conotoxin_omega-typ_CS.
[Graphical view]
PfamiPF02950. Conotoxin. 1 hit.
[Graphical view]
PROSITEiPS60004. OMEGA_CONOTOXIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01522-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLTCVVIVA VLLLTACQLI TADDSRGTQK HRALGSTTEL SLSTRCKSPG
60 70
SSCSPTSYNC CRSCNPYTKR CYG
Length:73
Mass (Da):7,851
Last modified:February 1, 1994 - v2
Checksum:i51A8C8FA630F7175
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84612 mRNA. Translation: AAA81590.1.
PIRiA44006. NTKN6G.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84612 mRNA. Translation: AAA81590.1.
PIRiA44006. NTKN6G.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OMCNMR-A46-72[»]
1TR6NMR-A46-72[»]
1TTLNMR-A46-72[»]
2CCONMR-A46-72[»]
ProteinModelPortaliP01522.
SMRiP01522. Positions 46-72.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP01522. 1 interaction.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

ConoServeri820. GVIA precursor.

Miscellaneous databases

EvolutionaryTraceiP01522.

Family and domain databases

InterProiIPR004214. Conotoxin.
IPR012321. Conotoxin_omega-typ_CS.
[Graphical view]
PfamiPF02950. Conotoxin. 1 hit.
[Graphical view]
PROSITEiPS60004. OMEGA_CONOTOXIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCO16A_CONGE
AccessioniPrimary (citable) accession number: P01522
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: February 1, 1994
Last modified: December 9, 2015
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.